(Circular) (Six-base) MAPSORT of: pF1KE0054.seq Check: 7147 from: 1 to: 6152 pF1KE0054 6152 bp With 182 enzymes: SgfI * September 11, 2017 11:55 .. AccI GT'mk_AC Cuts at: 2804 3190 2804 Size: 386 5766 Acc65I G'GTAC_C Cuts at: 3168 3168 Size: 6152 AclI AA'CG_TT Cuts at: 5992 5992 Size: 6152 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 269 464 2381 2392 2712 3977 4409 5156 5424 Size: 195 1917 11 320 1265 432 747 268 Cuts at: 5424 269 Size: 997 Fragments arranged by size: 1917 1265 997 747 432 320 268 195 11 AflIII A'CryG_T Cuts at: 3504 4608 5585 3504 Size: 1104 977 4071 Fragments arranged by size: 4071 1104 977 AgeI A'CCGG_T Cuts at: 4540 4540 Size: 6152 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1307 1523 1307 Size: 216 5936 AlwNI CAG_nnn'CTG Cuts at: 1531 3457 5024 1531 Size: 1926 1567 2659 Fragments arranged by size: 2659 1926 1567 ApaI G_GGCC'C Cuts at: 2874 2874 Size: 6152 ApaLI G'TGCA_C Cuts at: 880 2581 2857 4922 880 Size: 1701 276 2065 2110 Fragments arranged by size: 2110 2065 1701 276 ApoI r'AATT_y Cuts at: 829 1169 1326 1339 1604 1921 3156 5505 5761 Size: 340 157 13 265 317 1235 2349 256 Cuts at: 5761 829 Size: 1220 Fragments arranged by size: 2349 1235 1220 340 317 265 256 157 13 AseI AT'TA_AT Cuts at: 20 20 Size: 6152 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6152 AvaI C'yCGr_G Cuts at: 3074 3172 3074 Size: 98 6054 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1802 1835 2231 2264 1802 Size: 33 396 33 5690 Fragments arranged by size: 5690 396 33 33 BamHI G'GATC_C Cuts at: 3177 3177 Size: 6152 BanI G'GyrC_C Cuts at: 2632 3168 3582 3830 3865 2632 Size: 536 414 248 35 4919 Fragments arranged by size: 4919 536 414 248 35 BanII G_rGCy'C Cuts at: 199 262 1507 2874 3166 4196 199 Size: 63 1245 1367 292 1030 2155 Fragments arranged by size: 2155 1367 1245 1030 292 63 BbeI G_GCGC'C Cuts at: 3586 3834 3586 Size: 248 5904 BbsI GAAGACnn'nnnn_ Cuts at: 911 2620 2928 911 Size: 1709 308 4135 Fragments arranged by size: 4135 1709 308 BciVI GTATCCnnnnn_n' Cuts at: 4050 4811 6152 4050 Size: 761 1341 4050 Fragments arranged by size: 4050 1341 761 BclI T'GATC_A Cuts at: 2642 3054 3672 2642 Size: 412 618 5122 Fragments arranged by size: 5122 618 412 BfrBI ATG'CAT Cuts at: 1047 1047 Size: 6152 BglII A'GATC_T Cuts at: 2907 3667 5704 2907 Size: 760 2037 3355 Fragments arranged by size: 3355 2037 760 BlpI GC'TnA_GC Cuts at: 3264 3264 Size: 6152 Bme1580I G_kGCm'C Cuts at: 884 2585 2861 2874 3777 3870 4926 884 Size: 1701 276 13 903 93 1056 2110 Fragments arranged by size: 2110 1701 1056 903 276 93 13 BmrI ACTGGGnnnn_n' Cuts at: 1673 2627 3364 3445 3780 5924 1673 Size: 954 737 81 335 2144 1901 Fragments arranged by size: 2144 1901 954 737 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4519 4519 Size: 6152 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1264 1460 2228 2378 2619 1264 Size: 196 768 150 241 4797 Fragments arranged by size: 4797 768 241 196 150 Bpu10I CC'TnA_GC Cuts at: 2521 2521 Size: 6152 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3328 4207 4699 4997 3328 Size: 879 492 298 4483 Fragments arranged by size: 4483 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 592 5856 592 Size: 5264 888 BsaAI yAC'GTr Cuts at: 3505 3505 Size: 6152 BsaBI GATnn'nnATC Cuts at: 6 3666 5703 6 Size: 3660 2037 455 Fragments arranged by size: 3660 2037 455 BsaHI Gr'CG_yC Cuts at: 3583 3831 4533 6029 3583 Size: 248 702 1496 3706 Fragments arranged by size: 3706 1496 702 248 BsaWI w'CCGG_w Cuts at: 2143 3341 3862 4540 4814 4961 5372 2143 Size: 1198 521 678 274 147 411 2923 Fragments arranged by size: 2923 1198 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 746 776 1881 1911 2097 2127 2376 2406 746 Size: 30 1105 30 186 30 249 30 4492 Fragments arranged by size: 4492 1105 249 186 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 138 228 2102 138 Size: 90 1874 4188 Fragments arranged by size: 4188 1874 90 BseYI C'CCAG_C Cuts at: 3577 4335 4912 5408 3577 Size: 758 577 496 4321 Fragments arranged by size: 4321 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2318 2318 Size: 6152 BsiEI CG_ry'CG Cuts at: 104 3694 3740 4948 104 Size: 3590 46 1208 1308 Fragments arranged by size: 3590 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 262 856 884 1237 1507 2585 2861 3166 3944 Size: 594 28 353 270 1078 276 305 778 Cuts at: 3944 4134 4926 262 Size: 190 792 1488 Fragments arranged by size: 1488 1078 792 778 594 353 305 276 270 190 28 BsmI GAATG_Cn' Cuts at: 2850 2850 Size: 6152 BsmBI CGTCTCn'nnnn_ Cuts at: 2896 2896 Size: 6152 Bsp1286I G_dGCh'C Cuts at: 199 262 856 884 1237 1507 2585 2861 2874 Size: 63 594 28 353 270 1078 276 13 Cuts at: 2874 3166 3777 3870 3944 4134 4196 4926 199 Size: 292 611 93 74 190 62 730 1425 Fragments arranged by size: 1425 1078 730 611 594 353 292 276 270 190 93 74 63 62 28 13 BspEI T'CCGG_A Cuts at: 5372 5372 Size: 6152 BspHI T'CATG_A Cuts at: 5328 5328 Size: 6152 BspMI ACCTGCnnnn'nnnn_ Cuts at: 3202 3416 3718 4099 3202 Size: 214 302 381 5255 Fragments arranged by size: 5255 381 302 214 BsrBI CCG'CTC Cuts at: 50 4444 4498 5982 6147 50 Size: 4394 54 1484 165 55 Fragments arranged by size: 4394 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 976 4064 976 Size: 3088 3064 BsrFI r'CCGG_y Cuts at: 4150 4331 4540 4150 Size: 181 209 5762 Fragments arranged by size: 5762 209 181 BsrGI T'GTAC_A Cuts at: 2757 2757 Size: 6152 BssSI C'ACGA_G Cuts at: 4423 4781 4423 Size: 358 5794 BstAPI GCAn_nnn'nTGC Cuts at: 649 3529 649 Size: 2880 3272 BstBI TT'CG_AA Cuts at: 4513 4513 Size: 6152 BstXI CCAn_nnnn'nTGG Cuts at: 2402 2402 Size: 6152 BstYI r'GATC_y Cuts at: 1 417 1077 1405 2449 2732 2907 3104 3177 Size: 416 660 328 1044 283 175 197 73 Cuts at: 3177 3644 3667 4002 4248 5260 5346 5358 5704 Size: 467 23 335 246 1012 86 12 346 Cuts at: 5704 1 Size: 449 Fragments arranged by size: 1044 1012 660 467 449 416 346 335 328 283 246 197 175 86 73 23 12 Bsu36I CC'TnA_GG Cuts at: 449 449 Size: 6152 BtgI C'CryG_G Cuts at: 107 2852 107 Size: 2745 3407 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3054 4102 4283 4446 3054 Size: 1048 181 163 4760 Fragments arranged by size: 4760 1048 181 163 BtsI GCAGTG_nn' Cuts at: 3395 3395 Size: 6152 DraI TTT'AAA Cuts at: 834 2073 2103 3151 834 Size: 1239 30 1048 3835 Fragments arranged by size: 3835 1239 1048 30 DraIII CAC_nnn'GTG Cuts at: 2341 2341 Size: 6152 DrdI GACnn_nn'nnGTC Cuts at: 3858 4716 3858 Size: 858 5294 EaeI y'GGCC_r Cuts at: 125 1445 2404 3737 3911 4302 4329 5674 125 Size: 1320 959 1333 174 391 27 1345 603 Fragments arranged by size: 1345 1333 1320 959 603 391 174 27 EagI C'GGCC_G Cuts at: 3737 3737 Size: 6152 EarI CTCTTCn'nnn_ Cuts at: 220 483 575 1013 2711 4175 4385 220 Size: 263 92 438 1698 1464 210 1987 Fragments arranged by size: 1987 1698 1464 438 263 210 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 127 4360 4670 4816 5962 127 Size: 4233 310 146 1146 317 Fragments arranged by size: 4233 1146 317 310 146 EcoICRI GAG'CTC Cuts at: 260 1505 3164 260 Size: 1245 1659 3248 Fragments arranged by size: 3248 1659 1245 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 269 464 1264 1460 2228 2378 2381 2392 2619 Size: 195 800 196 768 150 3 11 227 Cuts at: 2619 2712 3977 4409 5156 5424 269 Size: 93 1265 432 747 268 997 Fragments arranged by size: 1265 997 800 768 747 432 268 227 196 195 150 93 11 3 EcoNI CCTnn'n_nnAGG Cuts at: 2234 2234 Size: 6152 EcoO109I rG'GnC_Cy Cuts at: 2007 2870 2871 3291 2007 Size: 863 1 420 4868 Fragments arranged by size: 4868 863 420 1 EcoRI G'AATT_C Cuts at: 1326 1339 1921 3156 1326 Size: 13 582 1235 4322 Fragments arranged by size: 4322 1235 582 13 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2084 4171 2084 Size: 2087 4065 FspI TGC'GCA Cuts at: 3933 3933 Size: 6152 HaeII r_GCGC'y Cuts at: 3586 3834 4856 5835 3586 Size: 248 1022 979 3903 Fragments arranged by size: 3903 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 265 297 880 912 1062 1094 1981 2013 3994 Size: 32 583 32 150 32 887 32 1981 Cuts at: 3994 4024 4026 4056 265 Size: 30 2 30 2361 Fragments arranged by size: 2361 1981 887 583 150 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2327 2916 3191 2327 Size: 589 275 5288 Fragments arranged by size: 5288 589 275 HindIII A'AGCT_T Cuts at: 928 2825 928 Size: 1897 4255 KasI G'GCGC_C Cuts at: 3582 3830 3582 Size: 248 5904 KpnI G_GTAC'C Cuts at: 3172 3172 Size: 6152 MfeI C'AATT_G Cuts at: 3092 3092 Size: 6152 MluI A'CGCG_T Cuts at: 5585 5585 Size: 6152 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 4823 5007 4823 Size: 184 5968 MscI TGG'CCA Cuts at: 1447 2406 3913 1447 Size: 959 1507 3686 Fragments arranged by size: 3686 1507 959 MslI CAynn'nnrTG Cuts at: 1004 1988 2135 2680 4141 4268 1004 Size: 984 147 545 1461 127 2888 Fragments arranged by size: 2888 1461 984 545 147 127 MspA1I CmG'CkG Cuts at: 3263 3577 3937 4950 5195 3263 Size: 314 360 1013 245 4220 Fragments arranged by size: 4220 1013 360 314 245 NaeI GCC'GGC Cuts at: 4333 4333 Size: 6152 NarI GG'CG_CC Cuts at: 3583 3831 3583 Size: 248 5904 NcoI C'CATG_G Cuts at: 107 2852 107 Size: 2745 3407 NdeI CA'TA_TG Cuts at: 2418 2418 Size: 6152 NgoMIV G'CCGG_C Cuts at: 4331 4331 Size: 6152 NsiI A_TGCA'T Cuts at: 1049 1049 Size: 6152 NspI r_CATG'y Cuts at: 1796 2551 3205 3705 4612 1796 Size: 755 654 500 907 3336 Fragments arranged by size: 3336 907 755 654 500 PasI CC'CwG_GG Cuts at: 216 216 Size: 6152 PciI A'CATG_T Cuts at: 4608 4608 Size: 6152 PmeI GTTT'AAAC Cuts at: 3151 3151 Size: 6152 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5291 5323 5291 Size: 32 6120 PpuMI rG'GwC_Cy Cuts at: 2007 2007 Size: 6152 PspOMI G'GGCC_C Cuts at: 2870 2870 Size: 6152 PstI C_TGCA'G Cuts at: 235 3199 3884 235 Size: 2964 685 2503 Fragments arranged by size: 2964 2503 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 6152 PvuII CAG'CTG Cuts at: 3577 3937 3577 Size: 360 5792 RsrII CG'GwC_CG Cuts at: 4347 4347 Size: 6152 SacI G_AGCT'C Cuts at: 262 1507 3166 262 Size: 1245 1659 3248 Fragments arranged by size: 3248 1659 1245 SalI G'TCGA_C Cuts at: 3189 3189 Size: 6152 SapI GCTCTTCn'nnn_ Cuts at: 220 575 2711 4175 4385 220 Size: 355 2136 1464 210 1987 Fragments arranged by size: 2136 1987 1464 355 210 SbfI CC_TGCA'GG Cuts at: 3199 3199 Size: 6152 ScaI AGT'ACT Cuts at: 700 2369 5669 700 Size: 1669 3300 1183 Fragments arranged by size: 3300 1669 1183 SfcI C'TryA_G Cuts at: 33 231 1136 2117 3195 3880 4873 5064 33 Size: 198 905 981 1078 685 993 191 1121 Fragments arranged by size: 1121 1078 993 981 905 685 198 191 SfoI GGC'GCC Cuts at: 3584 3832 3584 Size: 248 5904 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6152 SmaI CCC'GGG Cuts at: 3174 3174 Size: 6152 SmlI C'TyrA_G Cuts at: 3307 4222 4714 4976 3307 Size: 915 492 262 4483 Fragments arranged by size: 4483 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6152 SphI G_CATG'C Cuts at: 3205 3705 3205 Size: 500 5652 StyI C'CwwG_G Cuts at: 107 2235 2852 3286 3640 107 Size: 2128 617 434 354 2619 Fragments arranged by size: 2619 2128 617 434 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1120 3735 3868 4327 4536 1120 Size: 2615 133 459 209 2736 Fragments arranged by size: 2736 2615 459 209 133 TatI w'GTAC_w Cuts at: 698 2367 2757 3038 5667 698 Size: 1669 390 281 2629 1183 Fragments arranged by size: 2629 1669 1183 390 281 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2376 2408 5669 5701 2376 Size: 32 3261 32 2827 Fragments arranged by size: 3261 2827 32 32 Tth111I GACn'n_nGTC Cuts at: 3087 3949 3087 Size: 862 5290 XbaI T'CTAG_A Cuts at: 423 3183 423 Size: 2760 3392 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1455 3647 1455 Size: 2192 3960 XmaI C'CCGG_G Cuts at: 3172 3172 Size: 6152 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BbvCI BcgI BglI BmgBI BmtI BsiWI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI NheI NotI NruI PacI PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI