Restriction map for
pF1KE0054
(Circular) (Six-base) MAPSORT of: pF1KE0054.seq Check: 7147 from: 1 to: 6152
pF1KE0054 6152 bp
With 182 enzymes: SgfI *
September 11, 2017 11:55 ..
AccI GT'mk_AC
Cuts at: 2804 3190 2804
Size: 386 5766
Acc65I G'GTAC_C
Cuts at: 3168 3168
Size: 6152
AclI AA'CG_TT
Cuts at: 5992 5992
Size: 6152
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 464 2381 2392 2712 3977 4409 5156 5424
Size: 195 1917 11 320 1265 432 747 268
Cuts at: 5424 269
Size: 997
Fragments arranged by size:
1917 1265 997 747 432 320 268 195
11
AflIII A'CryG_T
Cuts at: 3504 4608 5585 3504
Size: 1104 977 4071
Fragments arranged by size:
4071 1104 977
AgeI A'CCGG_T
Cuts at: 4540 4540
Size: 6152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1307 1523 1307
Size: 216 5936
AlwNI CAG_nnn'CTG
Cuts at: 1531 3457 5024 1531
Size: 1926 1567 2659
Fragments arranged by size:
2659 1926 1567
ApaI G_GGCC'C
Cuts at: 2874 2874
Size: 6152
ApaLI G'TGCA_C
Cuts at: 880 2581 2857 4922 880
Size: 1701 276 2065 2110
Fragments arranged by size:
2110 2065 1701 276
ApoI r'AATT_y
Cuts at: 829 1169 1326 1339 1604 1921 3156 5505 5761
Size: 340 157 13 265 317 1235 2349 256
Cuts at: 5761 829
Size: 1220
Fragments arranged by size:
2349 1235 1220 340 317 265 256 157
13
AseI AT'TA_AT
Cuts at: 20 20
Size: 6152
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6152
AvaI C'yCGr_G
Cuts at: 3074 3172 3074
Size: 98 6054
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1802 1835 2231 2264 1802
Size: 33 396 33 5690
Fragments arranged by size:
5690 396 33 33
BamHI G'GATC_C
Cuts at: 3177 3177
Size: 6152
BanI G'GyrC_C
Cuts at: 2632 3168 3582 3830 3865 2632
Size: 536 414 248 35 4919
Fragments arranged by size:
4919 536 414 248 35
BanII G_rGCy'C
Cuts at: 199 262 1507 2874 3166 4196 199
Size: 63 1245 1367 292 1030 2155
Fragments arranged by size:
2155 1367 1245 1030 292 63
BbeI G_GCGC'C
Cuts at: 3586 3834 3586
Size: 248 5904
BbsI GAAGACnn'nnnn_
Cuts at: 911 2620 2928 911
Size: 1709 308 4135
Fragments arranged by size:
4135 1709 308
BciVI GTATCCnnnnn_n'
Cuts at: 4050 4811 6152 4050
Size: 761 1341 4050
Fragments arranged by size:
4050 1341 761
BclI T'GATC_A
Cuts at: 2642 3054 3672 2642
Size: 412 618 5122
Fragments arranged by size:
5122 618 412
BfrBI ATG'CAT
Cuts at: 1047 1047
Size: 6152
BglII A'GATC_T
Cuts at: 2907 3667 5704 2907
Size: 760 2037 3355
Fragments arranged by size:
3355 2037 760
BlpI GC'TnA_GC
Cuts at: 3264 3264
Size: 6152
Bme1580I G_kGCm'C
Cuts at: 884 2585 2861 2874 3777 3870 4926 884
Size: 1701 276 13 903 93 1056 2110
Fragments arranged by size:
2110 1701 1056 903 276 93 13
BmrI ACTGGGnnnn_n'
Cuts at: 1673 2627 3364 3445 3780 5924 1673
Size: 954 737 81 335 2144 1901
Fragments arranged by size:
2144 1901 954 737 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4519 4519
Size: 6152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 1460 2228 2378 2619 1264
Size: 196 768 150 241 4797
Fragments arranged by size:
4797 768 241 196 150
Bpu10I CC'TnA_GC
Cuts at: 2521 2521
Size: 6152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3328 4207 4699 4997 3328
Size: 879 492 298 4483
Fragments arranged by size:
4483 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 592 5856 592
Size: 5264 888
BsaAI yAC'GTr
Cuts at: 3505 3505
Size: 6152
BsaBI GATnn'nnATC
Cuts at: 6 3666 5703 6
Size: 3660 2037 455
Fragments arranged by size:
3660 2037 455
BsaHI Gr'CG_yC
Cuts at: 3583 3831 4533 6029 3583
Size: 248 702 1496 3706
Fragments arranged by size:
3706 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2143 3341 3862 4540 4814 4961 5372 2143
Size: 1198 521 678 274 147 411 2923
Fragments arranged by size:
2923 1198 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 746 776 1881 1911 2097 2127 2376 2406 746
Size: 30 1105 30 186 30 249 30 4492
Fragments arranged by size:
4492 1105 249 186 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 138 228 2102 138
Size: 90 1874 4188
Fragments arranged by size:
4188 1874 90
BseYI C'CCAG_C
Cuts at: 3577 4335 4912 5408 3577
Size: 758 577 496 4321
Fragments arranged by size:
4321 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2318 2318
Size: 6152
BsiEI CG_ry'CG
Cuts at: 104 3694 3740 4948 104
Size: 3590 46 1208 1308
Fragments arranged by size:
3590 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 262 856 884 1237 1507 2585 2861 3166 3944
Size: 594 28 353 270 1078 276 305 778
Cuts at: 3944 4134 4926 262
Size: 190 792 1488
Fragments arranged by size:
1488 1078 792 778 594 353 305 276
270 190 28
BsmI GAATG_Cn'
Cuts at: 2850 2850
Size: 6152
BsmBI CGTCTCn'nnnn_
Cuts at: 2896 2896
Size: 6152
Bsp1286I G_dGCh'C
Cuts at: 199 262 856 884 1237 1507 2585 2861 2874
Size: 63 594 28 353 270 1078 276 13
Cuts at: 2874 3166 3777 3870 3944 4134 4196 4926 199
Size: 292 611 93 74 190 62 730 1425
Fragments arranged by size:
1425 1078 730 611 594 353 292 276
270 190 93 74 63 62 28 13
BspEI T'CCGG_A
Cuts at: 5372 5372
Size: 6152
BspHI T'CATG_A
Cuts at: 5328 5328
Size: 6152
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3202 3416 3718 4099 3202
Size: 214 302 381 5255
Fragments arranged by size:
5255 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4444 4498 5982 6147 50
Size: 4394 54 1484 165 55
Fragments arranged by size:
4394 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 976 4064 976
Size: 3088 3064
BsrFI r'CCGG_y
Cuts at: 4150 4331 4540 4150
Size: 181 209 5762
Fragments arranged by size:
5762 209 181
BsrGI T'GTAC_A
Cuts at: 2757 2757
Size: 6152
BssSI C'ACGA_G
Cuts at: 4423 4781 4423
Size: 358 5794
BstAPI GCAn_nnn'nTGC
Cuts at: 649 3529 649
Size: 2880 3272
BstBI TT'CG_AA
Cuts at: 4513 4513
Size: 6152
BstXI CCAn_nnnn'nTGG
Cuts at: 2402 2402
Size: 6152
BstYI r'GATC_y
Cuts at: 1 417 1077 1405 2449 2732 2907 3104 3177
Size: 416 660 328 1044 283 175 197 73
Cuts at: 3177 3644 3667 4002 4248 5260 5346 5358 5704
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5704 1
Size: 449
Fragments arranged by size:
1044 1012 660 467 449 416 346 335
328 283 246 197 175 86 73 23
12
Bsu36I CC'TnA_GG
Cuts at: 449 449
Size: 6152
BtgI C'CryG_G
Cuts at: 107 2852 107
Size: 2745 3407
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3054 4102 4283 4446 3054
Size: 1048 181 163 4760
Fragments arranged by size:
4760 1048 181 163
BtsI GCAGTG_nn'
Cuts at: 3395 3395
Size: 6152
DraI TTT'AAA
Cuts at: 834 2073 2103 3151 834
Size: 1239 30 1048 3835
Fragments arranged by size:
3835 1239 1048 30
DraIII CAC_nnn'GTG
Cuts at: 2341 2341
Size: 6152
DrdI GACnn_nn'nnGTC
Cuts at: 3858 4716 3858
Size: 858 5294
EaeI y'GGCC_r
Cuts at: 125 1445 2404 3737 3911 4302 4329 5674 125
Size: 1320 959 1333 174 391 27 1345 603
Fragments arranged by size:
1345 1333 1320 959 603 391 174 27
EagI C'GGCC_G
Cuts at: 3737 3737
Size: 6152
EarI CTCTTCn'nnn_
Cuts at: 220 483 575 1013 2711 4175 4385 220
Size: 263 92 438 1698 1464 210 1987
Fragments arranged by size:
1987 1698 1464 438 263 210 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 4360 4670 4816 5962 127
Size: 4233 310 146 1146 317
Fragments arranged by size:
4233 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 260 1505 3164 260
Size: 1245 1659 3248
Fragments arranged by size:
3248 1659 1245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 464 1264 1460 2228 2378 2381 2392 2619
Size: 195 800 196 768 150 3 11 227
Cuts at: 2619 2712 3977 4409 5156 5424 269
Size: 93 1265 432 747 268 997
Fragments arranged by size:
1265 997 800 768 747 432 268 227
196 195 150 93 11 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2234 2234
Size: 6152
EcoO109I rG'GnC_Cy
Cuts at: 2007 2870 2871 3291 2007
Size: 863 1 420 4868
Fragments arranged by size:
4868 863 420 1
EcoRI G'AATT_C
Cuts at: 1326 1339 1921 3156 1326
Size: 13 582 1235 4322
Fragments arranged by size:
4322 1235 582 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2084 4171 2084
Size: 2087 4065
FspI TGC'GCA
Cuts at: 3933 3933
Size: 6152
HaeII r_GCGC'y
Cuts at: 3586 3834 4856 5835 3586
Size: 248 1022 979 3903
Fragments arranged by size:
3903 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 265 297 880 912 1062 1094 1981 2013 3994
Size: 32 583 32 150 32 887 32 1981
Cuts at: 3994 4024 4026 4056 265
Size: 30 2 30 2361
Fragments arranged by size:
2361 1981 887 583 150 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2327 2916 3191 2327
Size: 589 275 5288
Fragments arranged by size:
5288 589 275
HindIII A'AGCT_T
Cuts at: 928 2825 928
Size: 1897 4255
KasI G'GCGC_C
Cuts at: 3582 3830 3582
Size: 248 5904
KpnI G_GTAC'C
Cuts at: 3172 3172
Size: 6152
MfeI C'AATT_G
Cuts at: 3092 3092
Size: 6152
MluI A'CGCG_T
Cuts at: 5585 5585
Size: 6152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4823 5007 4823
Size: 184 5968
MscI TGG'CCA
Cuts at: 1447 2406 3913 1447
Size: 959 1507 3686
Fragments arranged by size:
3686 1507 959
MslI CAynn'nnrTG
Cuts at: 1004 1988 2135 2680 4141 4268 1004
Size: 984 147 545 1461 127 2888
Fragments arranged by size:
2888 1461 984 545 147 127
MspA1I CmG'CkG
Cuts at: 3263 3577 3937 4950 5195 3263
Size: 314 360 1013 245 4220
Fragments arranged by size:
4220 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4333 4333
Size: 6152
NarI GG'CG_CC
Cuts at: 3583 3831 3583
Size: 248 5904
NcoI C'CATG_G
Cuts at: 107 2852 107
Size: 2745 3407
NdeI CA'TA_TG
Cuts at: 2418 2418
Size: 6152
NgoMIV G'CCGG_C
Cuts at: 4331 4331
Size: 6152
NsiI A_TGCA'T
Cuts at: 1049 1049
Size: 6152
NspI r_CATG'y
Cuts at: 1796 2551 3205 3705 4612 1796
Size: 755 654 500 907 3336
Fragments arranged by size:
3336 907 755 654 500
PasI CC'CwG_GG
Cuts at: 216 216
Size: 6152
PciI A'CATG_T
Cuts at: 4608 4608
Size: 6152
PmeI GTTT'AAAC
Cuts at: 3151 3151
Size: 6152
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5291 5323 5291
Size: 32 6120
PpuMI rG'GwC_Cy
Cuts at: 2007 2007
Size: 6152
PspOMI G'GGCC_C
Cuts at: 2870 2870
Size: 6152
PstI C_TGCA'G
Cuts at: 235 3199 3884 235
Size: 2964 685 2503
Fragments arranged by size:
2964 2503 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6152
PvuII CAG'CTG
Cuts at: 3577 3937 3577
Size: 360 5792
RsrII CG'GwC_CG
Cuts at: 4347 4347
Size: 6152
SacI G_AGCT'C
Cuts at: 262 1507 3166 262
Size: 1245 1659 3248
Fragments arranged by size:
3248 1659 1245
SalI G'TCGA_C
Cuts at: 3189 3189
Size: 6152
SapI GCTCTTCn'nnn_
Cuts at: 220 575 2711 4175 4385 220
Size: 355 2136 1464 210 1987
Fragments arranged by size:
2136 1987 1464 355 210
SbfI CC_TGCA'GG
Cuts at: 3199 3199
Size: 6152
ScaI AGT'ACT
Cuts at: 700 2369 5669 700
Size: 1669 3300 1183
Fragments arranged by size:
3300 1669 1183
SfcI C'TryA_G
Cuts at: 33 231 1136 2117 3195 3880 4873 5064 33
Size: 198 905 981 1078 685 993 191 1121
Fragments arranged by size:
1121 1078 993 981 905 685 198 191
SfoI GGC'GCC
Cuts at: 3584 3832 3584
Size: 248 5904
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6152
SmaI CCC'GGG
Cuts at: 3174 3174
Size: 6152
SmlI C'TyrA_G
Cuts at: 3307 4222 4714 4976 3307
Size: 915 492 262 4483
Fragments arranged by size:
4483 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6152
SphI G_CATG'C
Cuts at: 3205 3705 3205
Size: 500 5652
StyI C'CwwG_G
Cuts at: 107 2235 2852 3286 3640 107
Size: 2128 617 434 354 2619
Fragments arranged by size:
2619 2128 617 434 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1120 3735 3868 4327 4536 1120
Size: 2615 133 459 209 2736
Fragments arranged by size:
2736 2615 459 209 133
TatI w'GTAC_w
Cuts at: 698 2367 2757 3038 5667 698
Size: 1669 390 281 2629 1183
Fragments arranged by size:
2629 1669 1183 390 281
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2376 2408 5669 5701 2376
Size: 32 3261 32 2827
Fragments arranged by size:
3261 2827 32 32
Tth111I GACn'n_nGTC
Cuts at: 3087 3949 3087
Size: 862 5290
XbaI T'CTAG_A
Cuts at: 423 3183 423
Size: 2760 3392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1455 3647 1455
Size: 2192 3960
XmaI C'CCGG_G
Cuts at: 3172 3172
Size: 6152
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BbvCI BcgI BglI BmgBI BmtI BsiWI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI
NheI NotI NruI PacI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI