Restriction map for pF1KE0054
 (Circular) (Six-base) MAPSORT of: pF1KE0054.seq  Check: 7147  from: 1  to: 6152



pF1KE0054  6152 bp

 With 182 enzymes: SgfI * 

                        September 11, 2017 11:55  ..

AccI GT'mk_AC

Cuts at:   2804    3190    2804 
   Size:        386    5766
 
Acc65I G'GTAC_C

Cuts at:   3168    3168 
   Size:       6152
 
AclI AA'CG_TT

Cuts at:   5992    5992 
   Size:       6152
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    269     464    2381    2392    2712    3977    4409    5156    5424 
   Size:        195    1917      11     320    1265     432     747     268

Cuts at:   5424     269 
   Size:        997

  Fragments arranged by size: 

                1917    1265     997     747     432     320     268     195
                  11
 
AflIII A'CryG_T

Cuts at:   3504    4608    5585    3504 
   Size:       1104     977    4071

  Fragments arranged by size: 

                4071    1104     977
 
AgeI A'CCGG_T

Cuts at:   4540    4540 
   Size:       6152
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1307    1523    1307 
   Size:        216    5936
 
AlwNI CAG_nnn'CTG

Cuts at:   1531    3457    5024    1531 
   Size:       1926    1567    2659

  Fragments arranged by size: 

                2659    1926    1567
 
ApaI G_GGCC'C

Cuts at:   2874    2874 
   Size:       6152
 
ApaLI G'TGCA_C

Cuts at:    880    2581    2857    4922     880 
   Size:       1701     276    2065    2110

  Fragments arranged by size: 

                2110    2065    1701     276
 
ApoI r'AATT_y

Cuts at:    829    1169    1326    1339    1604    1921    3156    5505    5761 
   Size:        340     157      13     265     317    1235    2349     256

Cuts at:   5761     829 
   Size:       1220

  Fragments arranged by size: 

                2349    1235    1220     340     317     265     256     157
                  13
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6152
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6152
 
AvaI C'yCGr_G

Cuts at:   3074    3172    3074 
   Size:         98    6054
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1802    1835    2231    2264    1802 
   Size:         33     396      33    5690

  Fragments arranged by size: 

                5690     396      33      33
 
BamHI G'GATC_C

Cuts at:   3177    3177 
   Size:       6152
 
BanI G'GyrC_C

Cuts at:   2632    3168    3582    3830    3865    2632 
   Size:        536     414     248      35    4919

  Fragments arranged by size: 

                4919     536     414     248      35
 
BanII G_rGCy'C

Cuts at:    199     262    1507    2874    3166    4196     199 
   Size:         63    1245    1367     292    1030    2155

  Fragments arranged by size: 

                2155    1367    1245    1030     292      63
 
BbeI G_GCGC'C

Cuts at:   3586    3834    3586 
   Size:        248    5904
 
BbsI GAAGACnn'nnnn_

Cuts at:    911    2620    2928     911 
   Size:       1709     308    4135

  Fragments arranged by size: 

                4135    1709     308
 
BciVI GTATCCnnnnn_n'

Cuts at:   4050    4811    6152    4050 
   Size:        761    1341    4050

  Fragments arranged by size: 

                4050    1341     761
 
BclI T'GATC_A

Cuts at:   2642    3054    3672    2642 
   Size:        412     618    5122

  Fragments arranged by size: 

                5122     618     412
 
BfrBI ATG'CAT

Cuts at:   1047    1047 
   Size:       6152
 
BglII A'GATC_T

Cuts at:   2907    3667    5704    2907 
   Size:        760    2037    3355

  Fragments arranged by size: 

                3355    2037     760
 
BlpI GC'TnA_GC

Cuts at:   3264    3264 
   Size:       6152
 
Bme1580I G_kGCm'C

Cuts at:    884    2585    2861    2874    3777    3870    4926     884 
   Size:       1701     276      13     903      93    1056    2110

  Fragments arranged by size: 

                2110    1701    1056     903     276      93      13
 
BmrI ACTGGGnnnn_n'

Cuts at:   1673    2627    3364    3445    3780    5924    1673 
   Size:        954     737      81     335    2144    1901

  Fragments arranged by size: 

                2144    1901     954     737     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4519    4519 
   Size:       6152
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1264    1460    2228    2378    2619    1264 
   Size:        196     768     150     241    4797

  Fragments arranged by size: 

                4797     768     241     196     150
 
Bpu10I CC'TnA_GC

Cuts at:   2521    2521 
   Size:       6152
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3328    4207    4699    4997    3328 
   Size:        879     492     298    4483

  Fragments arranged by size: 

                4483     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    592    5856     592 
   Size:       5264     888
 
BsaAI yAC'GTr

Cuts at:   3505    3505 
   Size:       6152
 
BsaBI GATnn'nnATC

Cuts at:      6    3666    5703       6 
   Size:       3660    2037     455

  Fragments arranged by size: 

                3660    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   3583    3831    4533    6029    3583 
   Size:        248     702    1496    3706

  Fragments arranged by size: 

                3706    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2143    3341    3862    4540    4814    4961    5372    2143 
   Size:       1198     521     678     274     147     411    2923

  Fragments arranged by size: 

                2923    1198     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    746     776    1881    1911    2097    2127    2376    2406     746 
   Size:         30    1105      30     186      30     249      30    4492

  Fragments arranged by size: 

                4492    1105     249     186      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    138     228    2102     138 
   Size:         90    1874    4188

  Fragments arranged by size: 

                4188    1874      90
 
BseYI C'CCAG_C

Cuts at:   3577    4335    4912    5408    3577 
   Size:        758     577     496    4321

  Fragments arranged by size: 

                4321     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2318    2318 
   Size:       6152
 
BsiEI CG_ry'CG

Cuts at:    104    3694    3740    4948     104 
   Size:       3590      46    1208    1308

  Fragments arranged by size: 

                3590    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    262     856     884    1237    1507    2585    2861    3166    3944 
   Size:        594      28     353     270    1078     276     305     778

Cuts at:   3944    4134    4926     262 
   Size:        190     792    1488

  Fragments arranged by size: 

                1488    1078     792     778     594     353     305     276
                 270     190      28
 
BsmI GAATG_Cn'

Cuts at:   2850    2850 
   Size:       6152
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2896    2896 
   Size:       6152
 
Bsp1286I G_dGCh'C

Cuts at:    199     262     856     884    1237    1507    2585    2861    2874 
   Size:         63     594      28     353     270    1078     276      13

Cuts at:   2874    3166    3777    3870    3944    4134    4196    4926     199 
   Size:        292     611      93      74     190      62     730    1425

  Fragments arranged by size: 

                1425    1078     730     611     594     353     292     276
                 270     190      93      74      63      62      28      13
            
 
BspEI T'CCGG_A

Cuts at:   5372    5372 
   Size:       6152
 
BspHI T'CATG_A

Cuts at:   5328    5328 
   Size:       6152
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   3202    3416    3718    4099    3202 
   Size:        214     302     381    5255

  Fragments arranged by size: 

                5255     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    4444    4498    5982    6147      50 
   Size:       4394      54    1484     165      55

  Fragments arranged by size: 

                4394    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    976    4064     976 
   Size:       3088    3064
 
BsrFI r'CCGG_y

Cuts at:   4150    4331    4540    4150 
   Size:        181     209    5762

  Fragments arranged by size: 

                5762     209     181
 
BsrGI T'GTAC_A

Cuts at:   2757    2757 
   Size:       6152
 
BssSI C'ACGA_G

Cuts at:   4423    4781    4423 
   Size:        358    5794
 
BstAPI GCAn_nnn'nTGC

Cuts at:    649    3529     649 
   Size:       2880    3272
 
BstBI TT'CG_AA

Cuts at:   4513    4513 
   Size:       6152
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2402    2402 
   Size:       6152
 
BstYI r'GATC_y

Cuts at:      1     417    1077    1405    2449    2732    2907    3104    3177 
   Size:        416     660     328    1044     283     175     197      73

Cuts at:   3177    3644    3667    4002    4248    5260    5346    5358    5704 
   Size:        467      23     335     246    1012      86      12     346

Cuts at:   5704       1 
   Size:        449

  Fragments arranged by size: 

                1044    1012     660     467     449     416     346     335
                 328     283     246     197     175      86      73      23
                  12
 
Bsu36I CC'TnA_GG

Cuts at:    449     449 
   Size:       6152
 
BtgI C'CryG_G

Cuts at:    107    2852     107 
   Size:       2745    3407
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3054    4102    4283    4446    3054 
   Size:       1048     181     163    4760

  Fragments arranged by size: 

                4760    1048     181     163
 
BtsI GCAGTG_nn'

Cuts at:   3395    3395 
   Size:       6152
 
DraI TTT'AAA

Cuts at:    834    2073    2103    3151     834 
   Size:       1239      30    1048    3835

  Fragments arranged by size: 

                3835    1239    1048      30
 
DraIII CAC_nnn'GTG

Cuts at:   2341    2341 
   Size:       6152
 
DrdI GACnn_nn'nnGTC

Cuts at:   3858    4716    3858 
   Size:        858    5294
 
EaeI y'GGCC_r

Cuts at:    125    1445    2404    3737    3911    4302    4329    5674     125 
   Size:       1320     959    1333     174     391      27    1345     603

  Fragments arranged by size: 

                1345    1333    1320     959     603     391     174      27
            
 
EagI C'GGCC_G

Cuts at:   3737    3737 
   Size:       6152
 
EarI CTCTTCn'nnn_

Cuts at:    220     483     575    1013    2711    4175    4385     220 
   Size:        263      92     438    1698    1464     210    1987

  Fragments arranged by size: 

                1987    1698    1464     438     263     210      92
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    127    4360    4670    4816    5962     127 
   Size:       4233     310     146    1146     317

  Fragments arranged by size: 

                4233    1146     317     310     146
 
EcoICRI GAG'CTC

Cuts at:    260    1505    3164     260 
   Size:       1245    1659    3248

  Fragments arranged by size: 

                3248    1659    1245
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    269     464    1264    1460    2228    2378    2381    2392    2619 
   Size:        195     800     196     768     150       3      11     227

Cuts at:   2619    2712    3977    4409    5156    5424     269 
   Size:         93    1265     432     747     268     997

  Fragments arranged by size: 

                1265     997     800     768     747     432     268     227
                 196     195     150      93      11       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2234    2234 
   Size:       6152
 
EcoO109I rG'GnC_Cy

Cuts at:   2007    2870    2871    3291    2007 
   Size:        863       1     420    4868

  Fragments arranged by size: 

                4868     863     420       1
 
EcoRI G'AATT_C

Cuts at:   1326    1339    1921    3156    1326 
   Size:         13     582    1235    4322

  Fragments arranged by size: 

                4322    1235     582      13
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2084    4171    2084 
   Size:       2087    4065
 
FspI TGC'GCA

Cuts at:   3933    3933 
   Size:       6152
 
HaeII r_GCGC'y

Cuts at:   3586    3834    4856    5835    3586 
   Size:        248    1022     979    3903

  Fragments arranged by size: 

                3903    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    265     297     880     912    1062    1094    1981    2013    3994 
   Size:         32     583      32     150      32     887      32    1981

Cuts at:   3994    4024    4026    4056     265 
   Size:         30       2      30    2361

  Fragments arranged by size: 

                2361    1981     887     583     150      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   2327    2916    3191    2327 
   Size:        589     275    5288

  Fragments arranged by size: 

                5288     589     275
 
HindIII A'AGCT_T

Cuts at:    928    2825     928 
   Size:       1897    4255
 
KasI G'GCGC_C

Cuts at:   3582    3830    3582 
   Size:        248    5904
 
KpnI G_GTAC'C

Cuts at:   3172    3172 
   Size:       6152
 
MfeI C'AATT_G

Cuts at:   3092    3092 
   Size:       6152
 
MluI A'CGCG_T

Cuts at:   5585    5585 
   Size:       6152
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   4823    5007    4823 
   Size:        184    5968
 
MscI TGG'CCA

Cuts at:   1447    2406    3913    1447 
   Size:        959    1507    3686

  Fragments arranged by size: 

                3686    1507     959
 
MslI CAynn'nnrTG

Cuts at:   1004    1988    2135    2680    4141    4268    1004 
   Size:        984     147     545    1461     127    2888

  Fragments arranged by size: 

                2888    1461     984     545     147     127
 
MspA1I CmG'CkG

Cuts at:   3263    3577    3937    4950    5195    3263 
   Size:        314     360    1013     245    4220

  Fragments arranged by size: 

                4220    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   4333    4333 
   Size:       6152
 
NarI GG'CG_CC

Cuts at:   3583    3831    3583 
   Size:        248    5904
 
NcoI C'CATG_G

Cuts at:    107    2852     107 
   Size:       2745    3407
 
NdeI CA'TA_TG

Cuts at:   2418    2418 
   Size:       6152
 
NgoMIV G'CCGG_C

Cuts at:   4331    4331 
   Size:       6152
 
NsiI A_TGCA'T

Cuts at:   1049    1049 
   Size:       6152
 
NspI r_CATG'y

Cuts at:   1796    2551    3205    3705    4612    1796 
   Size:        755     654     500     907    3336

  Fragments arranged by size: 

                3336     907     755     654     500
 
PasI CC'CwG_GG

Cuts at:    216     216 
   Size:       6152
 
PciI A'CATG_T

Cuts at:   4608    4608 
   Size:       6152
 
PmeI GTTT'AAAC

Cuts at:   3151    3151 
   Size:       6152
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5291    5323    5291 
   Size:         32    6120
 
PpuMI rG'GwC_Cy

Cuts at:   2007    2007 
   Size:       6152
 
PspOMI G'GGCC_C

Cuts at:   2870    2870 
   Size:       6152
 
PstI C_TGCA'G

Cuts at:    235    3199    3884     235 
   Size:       2964     685    2503

  Fragments arranged by size: 

                2964    2503     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6152
 
PvuII CAG'CTG

Cuts at:   3577    3937    3577 
   Size:        360    5792
 
RsrII CG'GwC_CG

Cuts at:   4347    4347 
   Size:       6152
 
SacI G_AGCT'C

Cuts at:    262    1507    3166     262 
   Size:       1245    1659    3248

  Fragments arranged by size: 

                3248    1659    1245
 
SalI G'TCGA_C

Cuts at:   3189    3189 
   Size:       6152
 
SapI GCTCTTCn'nnn_

Cuts at:    220     575    2711    4175    4385     220 
   Size:        355    2136    1464     210    1987

  Fragments arranged by size: 

                2136    1987    1464     355     210
 
SbfI CC_TGCA'GG

Cuts at:   3199    3199 
   Size:       6152
 
ScaI AGT'ACT

Cuts at:    700    2369    5669     700 
   Size:       1669    3300    1183

  Fragments arranged by size: 

                3300    1669    1183
 
SfcI C'TryA_G

Cuts at:     33     231    1136    2117    3195    3880    4873    5064      33 
   Size:        198     905     981    1078     685     993     191    1121

  Fragments arranged by size: 

                1121    1078     993     981     905     685     198     191
            
 
SfoI GGC'GCC

Cuts at:   3584    3832    3584 
   Size:        248    5904
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6152
 
SmaI CCC'GGG

Cuts at:   3174    3174 
   Size:       6152
 
SmlI C'TyrA_G

Cuts at:   3307    4222    4714    4976    3307 
   Size:        915     492     262    4483

  Fragments arranged by size: 

                4483     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6152
 
SphI G_CATG'C

Cuts at:   3205    3705    3205 
   Size:        500    5652
 
StyI C'CwwG_G

Cuts at:    107    2235    2852    3286    3640     107 
   Size:       2128     617     434     354    2619

  Fragments arranged by size: 

                2619    2128     617     434     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1120    3735    3868    4327    4536    1120 
   Size:       2615     133     459     209    2736

  Fragments arranged by size: 

                2736    2615     459     209     133
 
TatI w'GTAC_w

Cuts at:    698    2367    2757    3038    5667     698 
   Size:       1669     390     281    2629    1183

  Fragments arranged by size: 

                2629    1669    1183     390     281
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2376    2408    5669    5701    2376 
   Size:         32    3261      32    2827

  Fragments arranged by size: 

                3261    2827      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3087    3949    3087 
   Size:        862    5290
 
XbaI T'CTAG_A

Cuts at:    423    3183     423 
   Size:       2760    3392
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1455    3647    1455 
   Size:       2192    3960
 
XmaI C'CCGG_G

Cuts at:   3172    3172 
   Size:       6152
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI     PasI     PciI
     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     AscI
    AvrII    BbvCI     BcgI     BglI    BmgBI     BmtI    BsiWI   BssHII
   BstEII  BstZ17I     ClaI    CspCI    EcoRV     FseI    FspAI     HpaI
     NheI     NotI     NruI     PacI    PflMI     PfoI     PmlI    PshAI
     PsiI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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