Restriction map for
pF1KE0050
(Circular) (Six-base) MAPSORT of: pF1KE0050.seq Check: 5436 from: 1 to: 4520
pF1KE0050 4520 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 600 1481 1558 600
Size: 881 77 3562
Fragments arranged by size:
3562 881 77
Acc65I G'GTAC_C
Cuts at: 1536 1536
Size: 4520
AclI AA'CG_TT
Cuts at: 4360 4360
Size: 4520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 1443 2345 2777 3524 3792 437
Size: 1006 902 432 747 268 1165
Fragments arranged by size:
1165 1006 902 747 432 268
AfeI AGC'GCT
Cuts at: 821 821
Size: 4520
AflIII A'CryG_T
Cuts at: 1015 1872 2976 3953 1015
Size: 857 1104 977 1582
Fragments arranged by size:
1582 1104 977 857
AgeI A'CCGG_T
Cuts at: 2908 2908
Size: 4520
AhdI GACnn_n'nnGTC
Cuts at: 788 1376 1460 788
Size: 588 84 3848
Fragments arranged by size:
3848 588 84
AleI CACnn'nnGTG
Cuts at: 746 746
Size: 4520
AlwNI CAG_nnn'CTG
Cuts at: 1825 3392 1825
Size: 1567 2953
ApaLI G'TGCA_C
Cuts at: 3290 3290
Size: 4520
ApoI r'AATT_y
Cuts at: 199 485 1524 3873 4129 199
Size: 286 1039 2349 256 590
Fragments arranged by size:
2349 1039 590 286 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
AvaI C'yCGr_G
Cuts at: 1540 1540
Size: 4520
BamHI G'GATC_C
Cuts at: 1545 1545
Size: 4520
BanI G'GyrC_C
Cuts at: 1536 1950 2198 2233 1536
Size: 414 248 35 3823
Fragments arranged by size:
3823 414 248 35
BanII G_rGCy'C
Cuts at: 1534 2564 1534
Size: 1030 3490
BbeI G_GCGC'C
Cuts at: 1954 2202 1954
Size: 248 4272
BbsI GAAGACnn'nnnn_
Cuts at: 121 121
Size: 4520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1356 1390 1356
Size: 34 4486
BciVI GTATCCnnnnn_n'
Cuts at: 2418 3179 4520 2418
Size: 761 1341 2418
Fragments arranged by size:
2418 1341 761
BclI T'GATC_A
Cuts at: 327 2040 327
Size: 1713 2807
BglII A'GATC_T
Cuts at: 2035 4072 2035
Size: 2037 2483
BlpI GC'TnA_GC
Cuts at: 1632 1632
Size: 4520
Bme1580I G_kGCm'C
Cuts at: 2145 2238 3294 2145
Size: 93 1056 3371
Fragments arranged by size:
3371 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1732 1813 2148 4292 1732
Size: 81 335 2144 1960
Fragments arranged by size:
2144 1960 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 4520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 935 1019 1103 1271 1355 1439 851
Size: 84 84 84 168 84 84 3932
Fragments arranged by size:
3932 168 84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1696 2575 3067 3365 1696
Size: 879 492 298 2851
Fragments arranged by size:
2851 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4224 4224
Size: 4520
BsaAI yAC'GTr
Cuts at: 1873 1873
Size: 4520
BsaBI GATnn'nnATC
Cuts at: 6 2034 4071 6
Size: 2028 2037 455
Fragments arranged by size:
2037 2028 455
BsaHI Gr'CG_yC
Cuts at: 1951 2199 2901 4397 1951
Size: 248 702 1496 2074
Fragments arranged by size:
2074 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1709 2230 2908 3182 3329 3740 1709
Size: 521 678 274 147 411 2489
Fragments arranged by size:
2489 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1945 2703 3280 3776 1945
Size: 758 577 496 2689
Fragments arranged by size:
2689 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2062 2108 3316 104
Size: 1958 46 1208 1308
Fragments arranged by size:
1958 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1534 2312 2502 3294 1534
Size: 778 190 792 2760
Fragments arranged by size:
2760 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1534 2145 2238 2312 2502 2564 3294 1534
Size: 611 93 74 190 62 730 2760
Fragments arranged by size:
2760 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3740 3740
Size: 4520
BspHI T'CATG_A
Cuts at: 3696 3696
Size: 4520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1570 1784 2086 2467 1570
Size: 214 302 381 3623
Fragments arranged by size:
3623 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2812 2866 4350 4515 50
Size: 2762 54 1484 165 55
Fragments arranged by size:
2762 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 166 2432 166
Size: 2266 2254
BsrFI r'CCGG_y
Cuts at: 2518 2699 2908 2518
Size: 181 209 4130
Fragments arranged by size:
4130 209 181
BssSI C'ACGA_G
Cuts at: 2791 3149 2791
Size: 358 4162
BstAPI GCAn_nnn'nTGC
Cuts at: 1897 1897
Size: 4520
BstBI TT'CG_AA
Cuts at: 2881 2881
Size: 4520
BstYI r'GATC_y
Cuts at: 1 1545 2012 2035 2370 2616 3628 3714 3726
Size: 1544 467 23 335 246 1012 86 12
Cuts at: 3726 4072 1
Size: 346 449
Fragments arranged by size:
1544 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 601 1482 601
Size: 881 3639
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1373 2470 2651 2814 1373
Size: 1097 181 163 3079
Fragments arranged by size:
3079 1097 181 163
BtsI GCAGTG_nn'
Cuts at: 1763 1763
Size: 4520
DraI TTT'AAA
Cuts at: 1519 1519
Size: 4520
DrdI GACnn_nn'nnGTC
Cuts at: 123 182 2226 3084 123
Size: 59 2044 858 1559
Fragments arranged by size:
2044 1559 858 59
EaeI y'GGCC_r
Cuts at: 270 2105 2279 2670 2697 4042 270
Size: 1835 174 391 27 1345 748
Fragments arranged by size:
1835 1345 748 391 174 27
EagI C'GGCC_G
Cuts at: 2105 2105
Size: 4520
EarI CTCTTCn'nnn_
Cuts at: 659 811 1100 2543 2753 659
Size: 152 289 1443 210 2426
Fragments arranged by size:
2426 1443 289 210 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2728 3038 3184 4330 2728
Size: 310 146 1146 2918
Fragments arranged by size:
2918 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1532 1532
Size: 4520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 851 935 1019 1103 1271 1355 1439 1443
Size: 414 84 84 84 168 84 84 4
Cuts at: 1443 2345 2777 3524 3792 437
Size: 902 432 747 268 1165
Fragments arranged by size:
1165 902 747 432 414 268 168 84
84 84 84 84 4
EcoO109I rG'GnC_Cy
Cuts at: 1659 1659
Size: 4520
EcoRI G'AATT_C
Cuts at: 1524 1524
Size: 4520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 382 2539 382
Size: 2157 2363
FspI TGC'GCA
Cuts at: 2301 2301
Size: 4520
HaeII r_GCGC'y
Cuts at: 823 1954 2202 3224 4203 823
Size: 1131 248 1022 979 1140
Fragments arranged by size:
1140 1131 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 774 806 1362 1394 1446 1478 2362 2392 2394
Size: 32 556 32 52 32 884 30 2
Cuts at: 2394 2424 774
Size: 30 2870
Fragments arranged by size:
2870 884 556 52 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 128 1559 128
Size: 1431 3089
HindIII A'AGCT_T
Cuts at: 375 375
Size: 4520
KasI G'GCGC_C
Cuts at: 1950 2198 1950
Size: 248 4272
KpnI G_GTAC'C
Cuts at: 1540 1540
Size: 4520
MluI A'CGCG_T
Cuts at: 3953 3953
Size: 4520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3191 3375 3191
Size: 184 4336
MscI TGG'CCA
Cuts at: 272 2281 272
Size: 2009 2511
MslI CAynn'nnrTG
Cuts at: 561 746 930 1266 2509 2636 561
Size: 185 184 336 1243 127 2445
Fragments arranged by size:
2445 1243 336 185 184 127
MspA1I CmG'CkG
Cuts at: 1631 1945 2305 3318 3563 1631
Size: 314 360 1013 245 2588
Fragments arranged by size:
2588 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2701 2701
Size: 4520
NarI GG'CG_CC
Cuts at: 1951 2199 1951
Size: 248 4272
NgoMIV G'CCGG_C
Cuts at: 2699 2699
Size: 4520
NspI r_CATG'y
Cuts at: 340 1019 1573 2073 2980 340
Size: 679 554 500 907 1880
Fragments arranged by size:
1880 907 679 554 500
PciI A'CATG_T
Cuts at: 1015 2976 1015
Size: 1961 2559
PmeI GTTT'AAAC
Cuts at: 1519 1519
Size: 4520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 3659
Size: 32 4488
PsiI TTA'TAA
Cuts at: 1396 1396
Size: 4520
PstI C_TGCA'G
Cuts at: 1567 2252 1567
Size: 685 3835
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4520
PvuII CAG'CTG
Cuts at: 1945 2305 1945
Size: 360 4160
RsrII CG'GwC_CG
Cuts at: 2715 2715
Size: 4520
SacI G_AGCT'C
Cuts at: 1534 1534
Size: 4520
SalI G'TCGA_C
Cuts at: 1557 1557
Size: 4520
SapI GCTCTTCn'nnn_
Cuts at: 2543 2753 2543
Size: 210 4310
SbfI CC_TGCA'GG
Cuts at: 1567 1567
Size: 4520
ScaI AGT'ACT
Cuts at: 4037 4037
Size: 4520
SfcI C'TryA_G
Cuts at: 33 1563 2248 3241 3432 33
Size: 1530 685 993 191 1121
Fragments arranged by size:
1530 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1952 2200 1952
Size: 248 4272
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
SmaI CCC'GGG
Cuts at: 1542 1542
Size: 4520
SmlI C'TyrA_G
Cuts at: 1675 2590 3082 3344 1675
Size: 915 492 262 2851
Fragments arranged by size:
2851 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4520
SphI G_CATG'C
Cuts at: 340 1573 2073 340
Size: 1233 500 2787
Fragments arranged by size:
2787 1233 500
StyI C'CwwG_G
Cuts at: 1654 2008 1654
Size: 354 4166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 297 1366 2103 2236 2695 2904 297
Size: 1069 737 133 459 209 1913
Fragments arranged by size:
1913 1069 737 459 209 133
TatI w'GTAC_w
Cuts at: 313 570 4035 313
Size: 257 3465 798
Fragments arranged by size:
3465 798 257
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1149 1181 4037 4069 1149
Size: 32 2856 32 1600
Fragments arranged by size:
2856 1600 32 32
Tth111I GACn'n_nGTC
Cuts at: 120 2317 120
Size: 2197 2323
XbaI T'CTAG_A
Cuts at: 1551 1551
Size: 4520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2015 2015
Size: 4520
XmaI C'CCGG_G
Cuts at: 1540 1540
Size: 4520
XmnI GAAnn'nnTTC
Cuts at: 531 531
Size: 4520
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI