Restriction map for
pF1KE0040
(Circular) (Six-base) MAPSORT of: pF1KE0040.seq Check: 3875 from: 1 to: 5021
pF1KE0040 5021 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1139 1139
Size: 5021
AccI GT'mk_AC
Cuts at: 2059 2059
Size: 5021
Acc65I G'GTAC_C
Cuts at: 2037 2037
Size: 5021
AclI AA'CG_TT
Cuts at: 4861 4861
Size: 5021
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1616 2846 3278 4025 4293 592
Size: 1024 1230 432 747 268 1320
Fragments arranged by size:
1320 1230 1024 747 432 268
AflIII A'CryG_T
Cuts at: 1925 2373 3477 4454 1925
Size: 448 1104 977 2492
Fragments arranged by size:
2492 1104 977 448
AgeI A'CCGG_T
Cuts at: 1653 3409 1653
Size: 1756 3265
AhdI GACnn_n'nnGTC
Cuts at: 1988 1988
Size: 5021
AleI CACnn'nnGTG
Cuts at: 652 1870 652
Size: 1218 3803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1075 1075
Size: 5021
AlwNI CAG_nnn'CTG
Cuts at: 255 540 1158 1425 2326 3893 255
Size: 285 618 267 901 1567 1383
Fragments arranged by size:
1567 1383 901 618 285 267
ApaI G_GGCC'C
Cuts at: 202 1864 202
Size: 1662 3359
ApaLI G'TGCA_C
Cuts at: 3791 3791
Size: 5021
ApoI r'AATT_y
Cuts at: 2025 4374 4630 2025
Size: 2349 256 2416
Fragments arranged by size:
2416 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5021
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5021
AvaI C'yCGr_G
Cuts at: 373 1856 2041 373
Size: 1483 185 3353
Fragments arranged by size:
3353 1483 185
BamHI G'GATC_C
Cuts at: 2046 2046
Size: 5021
BanI G'GyrC_C
Cuts at: 654 948 2037 2451 2699 2734 654
Size: 294 1089 414 248 35 2941
Fragments arranged by size:
2941 1089 414 294 248 35
BanII G_rGCy'C
Cuts at: 202 1857 1864 2035 3065 202
Size: 1655 7 171 1030 2158
Fragments arranged by size:
2158 1655 1030 171 7
BbeI G_GCGC'C
Cuts at: 2455 2703 2455
Size: 248 4773
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1932 1966 1932
Size: 34 4987
BciVI GTATCCnnnnn_n'
Cuts at: 1125 2919 3680 5021 1125
Size: 1794 761 1341 1125
Fragments arranged by size:
1794 1341 1125 761
BclI T'GATC_A
Cuts at: 2541 2541
Size: 5021
BglI GCCn_nnn'nGGC
Cuts at: 1720 1720
Size: 5021
BglII A'GATC_T
Cuts at: 2536 4573 2536
Size: 2037 2984
BlpI GC'TnA_GC
Cuts at: 2133 2133
Size: 5021
Bme1580I G_kGCm'C
Cuts at: 202 1864 2646 2739 3795 202
Size: 1662 782 93 1056 1428
Fragments arranged by size:
1662 1428 1056 782 93
BmgBI CAC'GTC
Cuts at: 510 510
Size: 5021
BmrI ACTGGGnnnn_n'
Cuts at: 2233 2314 2649 4793 2233
Size: 81 335 2144 2461
Fragments arranged by size:
2461 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3388 3388
Size: 5021
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 961 1341 1745 1825 517
Size: 444 380 404 80 3713
Fragments arranged by size:
3713 444 404 380 80
Bpu10I CC'TnA_GC
Cuts at: 1492 1783 1492
Size: 291 4730
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1672 2197 3076 3568 3866 1069
Size: 603 525 879 492 298 2224
Fragments arranged by size:
2224 879 603 525 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 625 4725 625
Size: 4100 921
BsaAI yAC'GTr
Cuts at: 1776 2374 1776
Size: 598 4423
BsaBI GATnn'nnATC
Cuts at: 6 1252 2535 4572 6
Size: 1246 1283 2037 455
Fragments arranged by size:
2037 1283 1246 455
BsaHI Gr'CG_yC
Cuts at: 2452 2700 3402 4898 2452
Size: 248 702 1496 2575
Fragments arranged by size:
2575 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1653 2210 2731 3409 3683 3830 4241 1653
Size: 557 521 678 274 147 411 2433
Fragments arranged by size:
2433 678 557 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 436 970 1397 1975 391
Size: 45 534 427 578 3437
Fragments arranged by size:
3437 578 534 427 45
BseYI C'CCAG_C
Cuts at: 182 527 602 732 1138 1415 1425 1532 1715
Size: 345 75 130 406 277 10 107 183
Cuts at: 1715 2446 3204 3781 4277 182
Size: 731 758 577 496 926
Fragments arranged by size:
926 758 731 577 496 406 345 277
183 130 107 75 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1484 1819 1484
Size: 335 4686
BsiEI CG_ry'CG
Cuts at: 104 306 505 2563 2609 3817 104
Size: 202 199 2058 46 1208 1308
Fragments arranged by size:
2058 1308 1208 202 199 46
BsiHKAI G_wGCw'C
Cuts at: 2035 2813 3003 3795 2035
Size: 778 190 792 3261
Fragments arranged by size:
3261 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 202 1857 1864 2035 2646 2739 2813 3003 3065
Size: 1655 7 171 611 93 74 190 62
Cuts at: 3065 3795 202
Size: 730 1428
Fragments arranged by size:
1655 1428 730 611 190 171 93 74
62 7
BspEI T'CCGG_A
Cuts at: 4241 4241
Size: 5021
BspHI T'CATG_A
Cuts at: 4197 4197
Size: 5021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 329 416 1057 1139 2071 2285 2587 2968 329
Size: 87 641 82 932 214 302 381 2382
Fragments arranged by size:
2382 932 641 381 302 214 87 82
BsrBI CCG'CTC
Cuts at: 50 1320 1617 3313 3367 4851 5016 50
Size: 1270 297 1696 54 1484 165 55
Fragments arranged by size:
1696 1484 1270 297 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2933 2933
Size: 5021
BsrFI r'CCGG_y
Cuts at: 305 1238 1653 3019 3200 3409 305
Size: 933 415 1366 181 209 1917
Fragments arranged by size:
1917 1366 933 415 209 181
BssSI C'ACGA_G
Cuts at: 3292 3650 3292
Size: 358 4663
BstAPI GCAn_nnn'nTGC
Cuts at: 255 1158 2398 255
Size: 903 1240 2878
Fragments arranged by size:
2878 1240 903
BstBI TT'CG_AA
Cuts at: 3382 3382
Size: 5021
BstEII G'GTnAC_C
Cuts at: 1743 1743
Size: 5021
BstXI CCAn_nnnn'nTGG
Cuts at: 732 1365 1505 1723 1872 732
Size: 633 140 218 149 3881
Fragments arranged by size:
3881 633 218 149 140
BstYI r'GATC_y
Cuts at: 1 326 425 470 1835 2046 2513 2536 2871
Size: 325 99 45 1365 211 467 23 335
Cuts at: 2871 3117 4129 4215 4227 4573 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1365 1012 467 449 346 335 325 246
211 99 86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 1524 1524
Size: 5021
BtgI C'CryG_G
Cuts at: 1637 1637
Size: 5021
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2971 3152 3315 2971
Size: 181 163 4677
Fragments arranged by size:
4677 181 163
BtsI GCAGTG_nn'
Cuts at: 934 1907 2264 934
Size: 973 357 3691
Fragments arranged by size:
3691 973 357
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1968 2003 1968
Size: 35 4986
DraI TTT'AAA
Cuts at: 984 2020 984
Size: 1036 3985
DraIII CAC_nnn'GTG
Cuts at: 1776 1776
Size: 5021
DrdI GACnn_nn'nnGTC
Cuts at: 2727 3585 2727
Size: 858 4163
EaeI y'GGCC_r
Cuts at: 303 502 931 1367 1904 2606 2780 3171 3198
Size: 199 429 436 537 702 174 391 27
Cuts at: 3198 4543 303
Size: 1345 781
Fragments arranged by size:
1345 781 702 537 436 429 391 199
174 27
EagI C'GGCC_G
Cuts at: 303 502 2606 303
Size: 199 2104 2718
Fragments arranged by size:
2718 2104 199
EarI CTCTTCn'nnn_
Cuts at: 109 183 3044 3254 109
Size: 74 2861 210 1876
Fragments arranged by size:
2861 1876 210 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 258 270 3229 3539 3685 4831 258
Size: 12 2959 310 146 1146 448
Fragments arranged by size:
2959 1146 448 310 146 12
EcoICRI GAG'CTC
Cuts at: 2033 2033
Size: 5021
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 592 961 1341 1616 1745 1825 2846 3278
Size: 75 369 380 275 129 80 1021 432
Cuts at: 3278 4025 4293 517
Size: 747 268 1245
Fragments arranged by size:
1245 1021 747 432 380 369 275 268
129 80 75
EcoNI CCTnn'n_nnAGG
Cuts at: 781 781
Size: 5021
EcoO109I rG'GnC_Cy
Cuts at: 198 384 819 1046 1142 1314 1392 1528 1860
Size: 186 435 227 96 172 78 136 332
Cuts at: 1860 1861 1884 2160 198
Size: 1 23 276 3059
Fragments arranged by size:
3059 435 332 276 227 186 172 136
96 78 23 1
EcoRI G'AATT_C
Cuts at: 2025 2025
Size: 5021
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 894 3040 894
Size: 2146 2875
FspI TGC'GCA
Cuts at: 2802 2802
Size: 5021
HaeII r_GCGC'y
Cuts at: 2455 2703 3725 4704 2455
Size: 248 1022 979 2772
Fragments arranged by size:
2772 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 946 978 1376 1392 1408 1424 1465 1497 1974
Size: 32 398 16 16 16 41 32 477
Cuts at: 1974 2006 2863 2893 2895 2925 946
Size: 32 857 30 2 30 3042
Fragments arranged by size:
3042 857 477 398 41 32 32 32
30 30 16 16 16 2
HincII GTy'rAC
Cuts at: 2060 2060
Size: 5021
HindIII A'AGCT_T
Cuts at: 1666 1666
Size: 5021
KasI G'GCGC_C
Cuts at: 2451 2699 2451
Size: 248 4773
KpnI G_GTAC'C
Cuts at: 2041 2041
Size: 5021
MluI A'CGCG_T
Cuts at: 4454 4454
Size: 5021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 100 1937 3692 3876 100
Size: 1837 1755 184 1245
Fragments arranged by size:
1837 1755 1245 184
MscI TGG'CCA
Cuts at: 933 1369 2782 933
Size: 436 1413 3172
Fragments arranged by size:
3172 1413 436
MslI CAynn'nnrTG
Cuts at: 652 1024 1040 1795 1870 3010 3137 652
Size: 372 16 755 75 1140 127 2536
Fragments arranged by size:
2536 1140 755 372 127 75 16
MspA1I CmG'CkG
Cuts at: 390 537 1072 1422 1719 1755 1803 1995 2132
Size: 147 535 350 297 36 48 192 137
Cuts at: 2132 2446 2806 3819 4064 390
Size: 314 360 1013 245 1347
Fragments arranged by size:
1347 1013 535 360 350 314 297 245
192 147 137 48 36
NaeI GCC'GGC
Cuts at: 307 3202 307
Size: 2895 2126
NarI GG'CG_CC
Cuts at: 2452 2700 2452
Size: 248 4773
NgoMIV G'CCGG_C
Cuts at: 305 3200 305
Size: 2895 2126
NspI r_CATG'y
Cuts at: 268 372 1207 1215 1929 2074 2574 3481 268
Size: 104 835 8 714 145 500 907 1808
Fragments arranged by size:
1808 907 835 714 500 145 104 8
PciI A'CATG_T
Cuts at: 1925 3477 1925
Size: 1552 3469
PflMI CCAn_nnn'nTGG
Cuts at: 540 1376 1776 540
Size: 836 400 3785
Fragments arranged by size:
3785 836 400
PfoI T'CCnGG_A
Cuts at: 794 794
Size: 5021
PmeI GTTT'AAAC
Cuts at: 2020 2020
Size: 5021
PmlI CAC'GTG
Cuts at: 1776 1776
Size: 5021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 4160
Size: 32 4989
PpuMI rG'GwC_Cy
Cuts at: 384 1046 1314 1392 1528 384
Size: 662 268 78 136 3877
Fragments arranged by size:
3877 662 268 136 78
PspOMI G'GGCC_C
Cuts at: 198 1860 198
Size: 1662 3359
PstI C_TGCA'G
Cuts at: 1902 2068 2753 1902
Size: 166 685 4170
Fragments arranged by size:
4170 685 166
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5021
PvuII CAG'CTG
Cuts at: 1072 1719 1755 1803 2446 2806 1072
Size: 647 36 48 643 360 3287
Fragments arranged by size:
3287 647 643 360 48 36
RsrII CG'GwC_CG
Cuts at: 3216 3216
Size: 5021
SacI G_AGCT'C
Cuts at: 2035 2035
Size: 5021
SalI G'TCGA_C
Cuts at: 2058 2058
Size: 5021
SanDI GG'GwC_CC
Cuts at: 384 1314 1392 384
Size: 930 78 4013
Fragments arranged by size:
4013 930 78
SapI GCTCTTCn'nnn_
Cuts at: 3044 3254 3044
Size: 210 4811
SbfI CC_TGCA'GG
Cuts at: 2068 2068
Size: 5021
ScaI AGT'ACT
Cuts at: 364 4538 364
Size: 4174 847
SfcI C'TryA_G
Cuts at: 33 1898 2064 2749 3742 3933 33
Size: 1865 166 685 993 191 1121
Fragments arranged by size:
1865 1121 993 685 191 166
SfoI GGC'GCC
Cuts at: 2453 2701 2453
Size: 248 4773
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5021
SmaI CCC'GGG
Cuts at: 2043 2043
Size: 5021
SmlI C'TyrA_G
Cuts at: 1084 1687 2176 3091 3583 3845 1084
Size: 603 489 915 492 262 2260
Fragments arranged by size:
2260 915 603 492 489 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5021
SphI G_CATG'C
Cuts at: 268 372 1207 2074 2574 268
Size: 104 835 867 500 2715
Fragments arranged by size:
2715 867 835 500 104
StuI AGG'CCT
Cuts at: 776 979 776
Size: 203 4818
StyI C'CwwG_G
Cuts at: 974 999 1739 2155 2509 974
Size: 25 740 416 354 3486
Fragments arranged by size:
3486 740 416 354 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2604 2737 3196 3405 2604
Size: 133 459 209 4220
Fragments arranged by size:
4220 459 209 133
TatI w'GTAC_w
Cuts at: 362 4536 362
Size: 4174 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1441 1473 4538 4570 1441
Size: 32 3065 32 1892
Fragments arranged by size:
3065 1892 32 32
Tth111I GACn'n_nGTC
Cuts at: 916 2818 916
Size: 1902 3119
XbaI T'CTAG_A
Cuts at: 2052 2052
Size: 5021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2516 2516
Size: 5021
XmaI C'CCGG_G
Cuts at: 2041 2041
Size: 5021
XmnI GAAnn'nnTTC
Cuts at: 691 691
Size: 5021
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BbsI
BbvCI BfrBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI