Restriction map for
pF1KE0036
(Circular) (Six-base) MAPSORT of: pF1KE0036.seq Check: 2155 from: 1 to: 5258
pF1KE0036 5258 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1216 1216
Size: 5258
AccI GT'mk_AC
Cuts at: 2296 2296
Size: 5258
Acc65I G'GTAC_C
Cuts at: 2274 2274
Size: 5258
AclI AA'CG_TT
Cuts at: 5098 5098
Size: 5258
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2157 3083 3515 4262 4530 2157
Size: 926 432 747 268 2885
Fragments arranged by size:
2885 926 747 432 268
AfeI AGC'GCT
Cuts at: 526 1126 526
Size: 600 4658
AflIII A'CryG_T
Cuts at: 2610 3714 4691 2610
Size: 1104 977 3177
Fragments arranged by size:
3177 1104 977
AgeI A'CCGG_T
Cuts at: 342 2143 3646 342
Size: 1801 1503 1954
Fragments arranged by size:
1954 1801 1503
AhdI GACnn_n'nnGTC
Cuts at: 324 324
Size: 5258
AleI CACnn'nnGTG
Cuts at: 454 454
Size: 5258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 993 993
Size: 5258
AlwNI CAG_nnn'CTG
Cuts at: 500 606 2057 2563 4130 500
Size: 106 1451 506 1567 1628
Fragments arranged by size:
1628 1567 1451 506 106
ApaI G_GGCC'C
Cuts at: 1149 1149
Size: 5258
ApaLI G'TGCA_C
Cuts at: 4028 4028
Size: 5258
ApoI r'AATT_y
Cuts at: 1860 1917 2111 2262 4611 4867 1860
Size: 57 194 151 2349 256 2251
Fragments arranged by size:
2349 2251 256 194 151 57
AseI AT'TA_AT
Cuts at: 20 20
Size: 5258
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
AvaI C'yCGr_G
Cuts at: 1433 1988 2039 2192 2278 1433
Size: 555 51 153 86 4413
Fragments arranged by size:
4413 555 153 86 51
BamHI G'GATC_C
Cuts at: 2283 2283
Size: 5258
BanI G'GyrC_C
Cuts at: 969 2274 2688 2936 2971 969
Size: 1305 414 248 35 3256
Fragments arranged by size:
3256 1305 414 248 35
BanII G_rGCy'C
Cuts at: 771 1149 1727 1847 1989 2040 2272 3302 771
Size: 378 578 120 142 51 232 1030 2727
Fragments arranged by size:
2727 1030 578 378 232 142 120 51
BbeI G_GCGC'C
Cuts at: 2692 2940 2692
Size: 248 5010
BbsI GAAGACnn'nnnn_
Cuts at: 1494 1494
Size: 5258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 274 308 1571 1605 90
Size: 34 150 34 1263 34 3743
Fragments arranged by size:
3743 1263 150 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2227 3156 3917 5258 2227
Size: 929 761 1341 2227
Fragments arranged by size:
2227 1341 929 761
BclI T'GATC_A
Cuts at: 2778 2778
Size: 5258
BfrBI ATG'CAT
Cuts at: 111 387 111
Size: 276 4982
BglI GCCn_nnn'nGGC
Cuts at: 841 841
Size: 5258
BglII A'GATC_T
Cuts at: 2773 4810 2773
Size: 2037 3221
BlpI GC'TnA_GC
Cuts at: 1873 1967 2370 1873
Size: 94 403 4761
Fragments arranged by size:
4761 403 94
Bme1580I G_kGCm'C
Cuts at: 175 1149 1457 2883 2976 4032 175
Size: 974 308 1426 93 1056 1401
Fragments arranged by size:
1426 1401 1056 974 308 93
BmrI ACTGGGnnnn_n'
Cuts at: 2470 2551 2886 5030 2470
Size: 81 335 2144 2698
Fragments arranged by size:
2698 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3625 3625
Size: 5258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 1522
Size: 5258
Bpu10I CC'TnA_GC
Cuts at: 2104 2104
Size: 5258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2434 3313 3805 4103 2434
Size: 879 492 298 3589
Fragments arranged by size:
3589 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 196 570 1068 4962 196
Size: 374 498 3894 492
Fragments arranged by size:
3894 498 492 374
BsaAI yAC'GTr
Cuts at: 2611 2611
Size: 5258
BsaBI GATnn'nnATC
Cuts at: 6 2772 4809 6
Size: 2766 2037 455
Fragments arranged by size:
2766 2037 455
BsaHI Gr'CG_yC
Cuts at: 1037 1317 2689 2937 3639 5135 1037
Size: 280 1372 248 702 1496 1160
Fragments arranged by size:
1496 1372 1160 702 280 248
BsaWI w'CCGG_w
Cuts at: 342 433 1088 2143 2447 2968 3646 3920 4067
Size: 91 655 1055 304 521 678 274 147
Cuts at: 4067 4478 342
Size: 411 1122
Fragments arranged by size:
1122 1055 678 655 521 411 304 274
147 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 169 199 1561 1591 169
Size: 30 1362 30 3836
Fragments arranged by size:
3836 1362 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 588 1177 1767 588
Size: 589 590 4079
Fragments arranged by size:
4079 590 589
BseYI C'CCAG_C
Cuts at: 120 158 180 425 667 881 1850 2683 3441
Size: 38 22 245 242 214 969 833 758
Cuts at: 3441 4018 4514 120
Size: 577 496 864
Fragments arranged by size:
969 864 833 758 577 496 245 242
214 38 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 1153
Size: 5258
BsiEI CG_ry'CG
Cuts at: 104 759 1143 2800 2846 4054 104
Size: 655 384 1657 46 1208 1308
Fragments arranged by size:
1657 1308 1208 655 384 46
BsiHKAI G_wGCw'C
Cuts at: 507 1292 1727 2272 3050 3240 4032 507
Size: 785 435 545 778 190 792 1733
Fragments arranged by size:
1733 792 785 778 545 435 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1308 2036 1308
Size: 728 4530
Bsp1286I G_dGCh'C
Cuts at: 175 507 771 1149 1292 1457 1727 1847 1989
Size: 332 264 378 143 165 270 120 142
Cuts at: 1989 2040 2272 2883 2976 3050 3240 3302 4032
Size: 51 232 611 93 74 190 62 730
Cuts at: 4032 175
Size: 1401
Fragments arranged by size:
1401 730 611 378 332 270 264 232
190 165 143 142 120 93 74 62
51
BspEI T'CCGG_A
Cuts at: 433 4478 433
Size: 4045 1213
BspHI T'CATG_A
Cuts at: 4434 4434
Size: 5258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 883 1175 1216 2308 2522 2824 3205 883
Size: 292 41 1092 214 302 381 2936
Fragments arranged by size:
2936 1092 381 302 292 214 41
BsrBI CCG'CTC
Cuts at: 50 965 1199 3550 3604 5088 5253 50
Size: 915 234 2351 54 1484 165 55
Fragments arranged by size:
2351 1484 915 234 165 55 54
BsrDI GCAATG_nn'
Cuts at: 687 3170 687
Size: 2483 2775
BsrFI r'CCGG_y
Cuts at: 342 1295 1733 2143 3256 3437 3646 342
Size: 953 438 410 1113 181 209 1954
Fragments arranged by size:
1954 1113 953 438 410 209 181
BsrGI T'GTAC_A
Cuts at: 1349 1349
Size: 5258
BssHII G'CGCG_C
Cuts at: 1204 1204
Size: 5258
BssSI C'ACGA_G
Cuts at: 3529 3887 3529
Size: 358 4900
BstAPI GCAn_nnn'nTGC
Cuts at: 1214 2635 1214
Size: 1421 3837
BstBI TT'CG_AA
Cuts at: 3619 3619
Size: 5258
BstXI CCAn_nnnn'nTGG
Cuts at: 243 315 496 1101 1716 243
Size: 72 181 605 615 3785
Fragments arranged by size:
3785 615 605 181 72
BstYI r'GATC_y
Cuts at: 1 908 2283 2750 2773 3108 3354 4366 4452
Size: 907 1375 467 23 335 246 1012 86
Cuts at: 4452 4464 4810 1
Size: 12 346 449
Fragments arranged by size:
1375 1012 907 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 314 759 1379 1486 1803 2153 314
Size: 445 620 107 317 350 3419
Fragments arranged by size:
3419 620 445 350 317 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1947 3208 3389 3552 1947
Size: 1261 181 163 3653
Fragments arranged by size:
3653 1261 181 163
BtsI GCAGTG_nn'
Cuts at: 203 790 848 1062 2501 203
Size: 587 58 214 1439 2960
Fragments arranged by size:
2960 1439 587 214 58
DraI TTT'AAA
Cuts at: 2257 2257
Size: 5258
DrdI GACnn_nn'nnGTC
Cuts at: 2964 3822 2964
Size: 858 4400
EaeI y'GGCC_r
Cuts at: 140 762 833 1112 1140 1301 1493 1524 1676
Size: 622 71 279 28 161 192 31 152
Cuts at: 1676 2843 3017 3408 3435 4780 140
Size: 1167 174 391 27 1345 618
Fragments arranged by size:
1345 1167 622 618 391 279 192 174
161 152 71 31 28 27
EagI C'GGCC_G
Cuts at: 1140 2843 1140
Size: 1703 3555
EarI CTCTTCn'nnn_
Cuts at: 268 404 2006 2028 3281 3491 268
Size: 136 1602 22 1253 210 2035
Fragments arranged by size:
2035 1602 1253 210 136 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1644 3466 3776 3922 5068 1644
Size: 1822 310 146 1146 1834
Fragments arranged by size:
1834 1822 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1725 2270 1725
Size: 545 4713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 2157 3083 3515 4262 4530 1522
Size: 635 926 432 747 268 2250
Fragments arranged by size:
2250 926 747 635 432 268
EcoO109I rG'GnC_Cy
Cuts at: 135 590 872 1146 1668 1996 2100 2397 135
Size: 455 282 274 522 328 104 297 2996
Fragments arranged by size:
2996 522 455 328 297 282 274 104
EcoRI G'AATT_C
Cuts at: 2262 2262
Size: 5258
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3277 3277
Size: 5258
FspI TGC'GCA
Cuts at: 3039 3039
Size: 5258
HaeII r_GCGC'y
Cuts at: 528 1128 1836 2692 2940 3962 4941 528
Size: 600 708 856 248 1022 979 845
Fragments arranged by size:
1022 979 856 845 708 600 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 310 342 628 660 1222 1254 2168
Size: 32 109 32 286 32 562 32 914
Cuts at: 2168 2200 3100 3130 3132 3162 169
Size: 32 900 30 2 30 2265
Fragments arranged by size:
2265 914 900 562 286 109 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 861 2297 861
Size: 1436 3822
KasI G'GCGC_C
Cuts at: 2688 2936 2688
Size: 248 5010
KpnI G_GTAC'C
Cuts at: 2278 2278
Size: 5258
MfeI C'AATT_G
Cuts at: 261 261
Size: 5258
MluI A'CGCG_T
Cuts at: 4691 4691
Size: 5258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 620 740 779 3929 4113 620
Size: 120 39 3150 184 1765
Fragments arranged by size:
3150 1765 184 120 39
MscI TGG'CCA
Cuts at: 142 1114 1303 1495 1526 1678 3019 142
Size: 972 189 192 31 152 1341 2381
Fragments arranged by size:
2381 1341 972 192 189 152 31
MslI CAynn'nnrTG
Cuts at: 454 778 975 1714 3247 3374 454
Size: 324 197 739 1533 127 2338
Fragments arranged by size:
2338 1533 739 324 197 127
MspA1I CmG'CkG
Cuts at: 288 299 429 474 885 1625 2369 2683 3043
Size: 11 130 45 411 740 744 314 360
Cuts at: 3043 4056 4301 288
Size: 1013 245 1245
Fragments arranged by size:
1245 1013 744 740 411 360 314 245
130 45 11
NaeI GCC'GGC
Cuts at: 3439 3439
Size: 5258
NarI GG'CG_CC
Cuts at: 2689 2937 2689
Size: 248 5010
NcoI C'CATG_G
Cuts at: 314 1379 314
Size: 1065 4193
NgoMIV G'CCGG_C
Cuts at: 3437 3437
Size: 5258
NsiI A_TGCA'T
Cuts at: 113 389 113
Size: 276 4982
NspI r_CATG'y
Cuts at: 391 1597 1788 2311 2811 3718 391
Size: 1206 191 523 500 907 1931
Fragments arranged by size:
1931 1206 907 523 500 191
PasI CC'CwG_GG
Cuts at: 190 1449 190
Size: 1259 3999
PciI A'CATG_T
Cuts at: 3714 3714
Size: 5258
PflMI CCAn_nnn'nTGG
Cuts at: 606 810 1899 606
Size: 204 1089 3965
Fragments arranged by size:
3965 1089 204
PfoI T'CCnGG_A
Cuts at: 509 1727 509
Size: 1218 4040
PmeI GTTT'AAAC
Cuts at: 2257 2257
Size: 5258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 4397 4429 2011
Size: 32 2354 32 2840
Fragments arranged by size:
2840 2354 32 32
PpuMI rG'GwC_Cy
Cuts at: 135 872 135
Size: 737 4521
PshAI GACnn'nnGTC
Cuts at: 208 1181 208
Size: 973 4285
PspOMI G'GGCC_C
Cuts at: 1145 1145
Size: 5258
PspXI vC'TCGA_Gb
Cuts at: 1988 1988
Size: 5258
PstI C_TGCA'G
Cuts at: 532 855 2212 2305 2990 532
Size: 323 1357 93 685 2800
Fragments arranged by size:
2800 1357 685 323 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5258
PvuII CAG'CTG
Cuts at: 474 885 1625 2683 3043 474
Size: 411 740 1058 360 2689
Fragments arranged by size:
2689 1058 740 411 360
RsrII CG'GwC_CG
Cuts at: 431 3453 431
Size: 3022 2236
SacI G_AGCT'C
Cuts at: 1727 2272 1727
Size: 545 4713
SalI G'TCGA_C
Cuts at: 2295 2295
Size: 5258
SapI GCTCTTCn'nnn_
Cuts at: 268 2028 3281 3491 268
Size: 1760 1253 210 2035
Fragments arranged by size:
2035 1760 1253 210
SbfI CC_TGCA'GG
Cuts at: 2305 2305
Size: 5258
ScaI AGT'ACT
Cuts at: 4775 4775
Size: 5258
SfcI C'TryA_G
Cuts at: 33 528 851 1458 2208 2301 2986 3979 4170
Size: 495 323 607 750 93 685 993 191
Cuts at: 4170 33
Size: 1121
Fragments arranged by size:
1121 993 750 685 607 495 323 191
93
SfiI GGCCn_nnn'nGGCC
Cuts at: 841 841
Size: 5258
SfoI GGC'GCC
Cuts at: 2690 2938 2690
Size: 248 5010
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
SmaI CCC'GGG
Cuts at: 1435 2280 1435
Size: 845 4413
SmlI C'TyrA_G
Cuts at: 1988 2413 3328 3820 4082 1988
Size: 425 915 492 262 3164
Fragments arranged by size:
3164 915 492 425 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5258
SphI G_CATG'C
Cuts at: 2311 2811 2311
Size: 500 4758
SrfI GCCC'GGGC
Cuts at: 1435 1435
Size: 5258
StyI C'CwwG_G
Cuts at: 314 1379 2392 2746 314
Size: 1065 1013 354 2826
Fragments arranged by size:
2826 1065 1013 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 198 930 1599 2841 2974 3433 3642 198
Size: 732 669 1242 133 459 209 1814
Fragments arranged by size:
1814 1242 732 669 459 209 133
TatI w'GTAC_w
Cuts at: 1349 4773 1349
Size: 3424 1834
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4775 4807 4775
Size: 32 5226
Tth111I GACn'n_nGTC
Cuts at: 3055 3055
Size: 5258
XbaI T'CTAG_A
Cuts at: 2289 2289
Size: 5258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 243 603 1446 2753 243
Size: 360 843 1307 2748
Fragments arranged by size:
2748 1307 843 360
XhoI C'TCGA_G
Cuts at: 1988 1988
Size: 5258
XmaI C'CCGG_G
Cuts at: 1433 2278 1433
Size: 845 4413
XmnI GAAnn'nnTTC
Cuts at: 2026 2026
Size: 5258
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BbvCI BmgBI
BmtI BsiWI BsmI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI PacI PmlI PsiI PsrI SacII
SanDI SexAI SgrAI SnaBI SspI StuI SwaI ZraI