Restriction map for
pF1KE0030
(Circular) (Six-base) MAPSORT of: pF1KE0030.seq Check: 9615 from: 1 to: 4769
pF1KE0030 4769 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1046 1046
Size: 4769
AatII G_ACGT'C
Cuts at: 443 443
Size: 4769
AccI GT'mk_AC
Cuts at: 1807 1807
Size: 4769
Acc65I G'GTAC_C
Cuts at: 1785 1785
Size: 4769
AclI AA'CG_TT
Cuts at: 4609 4609
Size: 4769
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 611 961 1555 1624 1695 2594 3026 3773
Size: 478 350 594 69 71 899 432 747
Cuts at: 3773 4041 133
Size: 268 861
Fragments arranged by size:
899 861 747 594 478 432 350 268
71 69
AfeI AGC'GCT
Cuts at: 934 1195 934
Size: 261 4508
AflIII A'CryG_T
Cuts at: 1043 2121 3225 4202 1043
Size: 1078 1104 977 1610
Fragments arranged by size:
1610 1104 1078 977
AgeI A'CCGG_T
Cuts at: 3157 3157
Size: 4769
AhdI GACnn_n'nnGTC
Cuts at: 1281 1281
Size: 4769
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1048 1048
Size: 4769
AlwNI CAG_nnn'CTG
Cuts at: 1740 2074 3641 1740
Size: 334 1567 2868
Fragments arranged by size:
2868 1567 334
ApaLI G'TGCA_C
Cuts at: 1144 3539 1144
Size: 2395 2374
ApoI r'AATT_y
Cuts at: 1773 4122 4378 1773
Size: 2349 256 2164
Fragments arranged by size:
2349 2164 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4769
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4769
AvaI C'yCGr_G
Cuts at: 1058 1286 1343 1789 1058
Size: 228 57 446 4038
Fragments arranged by size:
4038 446 228 57
BamHI G'GATC_C
Cuts at: 1794 1794
Size: 4769
BanI G'GyrC_C
Cuts at: 174 391 1066 1785 2199 2447 2482 174
Size: 217 675 719 414 248 35 2461
Fragments arranged by size:
2461 719 675 414 248 217 35
BanII G_rGCy'C
Cuts at: 577 1783 2813 577
Size: 1206 1030 2533
Fragments arranged by size:
2533 1206 1030
BbeI G_GCGC'C
Cuts at: 178 395 2203 2451 178
Size: 217 1808 248 2496
Fragments arranged by size:
2496 1808 248 217
BbsI GAAGACnn'nnnn_
Cuts at: 138 950 1581 138
Size: 812 631 3326
Fragments arranged by size:
3326 812 631
BbvCI CC'TCA_GC
Cuts at: 1501 1501
Size: 4769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4735
BciVI GTATCCnnnnn_n'
Cuts at: 2667 3428 4769 2667
Size: 761 1341 2667
Fragments arranged by size:
2667 1341 761
BclI T'GATC_A
Cuts at: 2289 2289
Size: 4769
BglI GCCn_nnn'nGGC
Cuts at: 1696 1696
Size: 4769
BglII A'GATC_T
Cuts at: 2284 4321 2284
Size: 2037 2732
BlpI GC'TnA_GC
Cuts at: 313 1881 313
Size: 1568 3201
Bme1580I G_kGCm'C
Cuts at: 434 1069 1148 2394 2487 3543 434
Size: 635 79 1246 93 1056 1660
Fragments arranged by size:
1660 1246 1056 635 93 79
BmgBI CAC'GTC
Cuts at: 1173 1278 1542 1557 1173
Size: 105 264 15 4385
Fragments arranged by size:
4385 264 105 15
BmrI ACTGGGnnnn_n'
Cuts at: 1981 2062 2397 4541 1981
Size: 81 335 2144 2209
Fragments arranged by size:
2209 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3136 3136
Size: 4769
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 920 951 1117 1258 1531 841
Size: 79 31 166 141 273 4079
Fragments arranged by size:
4079 273 166 141 79 31
Bpu10I CC'TnA_GC
Cuts at: 1501 1522 1501
Size: 21 4748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1945 2824 3316 3614 1945
Size: 879 492 298 3100
Fragments arranged by size:
3100 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 481 4473 481
Size: 3992 777
BsaAI yAC'GTr
Cuts at: 1044 1695 2122 1044
Size: 651 427 3691
Fragments arranged by size:
3691 651 427
BsaBI GATnn'nnATC
Cuts at: 6 1411 2283 4320 6
Size: 1405 872 2037 455
Fragments arranged by size:
2037 1405 872 455
BsaHI Gr'CG_yC
Cuts at: 118 175 197 392 440 752 806 2200 2448
Size: 57 22 195 48 312 54 1394 248
Cuts at: 2448 3150 4646 118
Size: 702 1496 241
Fragments arranged by size:
1496 1394 702 312 248 241 195 57
54 48 22
BsaWI w'CCGG_w
Cuts at: 1958 2479 3157 3431 3578 3989 1958
Size: 521 678 274 147 411 2738
Fragments arranged by size:
2738 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 949 979 949
Size: 30 4739
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 917 1708 917
Size: 791 3978
BseYI C'CCAG_C
Cuts at: 705 780 2194 2952 3529 4025 705
Size: 75 1414 758 577 496 1449
Fragments arranged by size:
1449 1414 758 577 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1024 1018
Size: 6 4763
BsiEI CG_ry'CG
Cuts at: 104 1640 2311 2357 3565 104
Size: 1536 671 46 1208 1308
Fragments arranged by size:
1536 1308 1208 671 46
BsiHKAI G_wGCw'C
Cuts at: 332 1148 1190 1517 1783 2561 2751 3543 332
Size: 816 42 327 266 778 190 792 1558
Fragments arranged by size:
1558 816 792 778 327 266 190 42
BsmI GAATG_Cn'
Cuts at: 273 1623 273
Size: 1350 3419
BsmBI CGTCTCn'nnnn_
Cuts at: 813 1093 813
Size: 280 4489
Bsp1286I G_dGCh'C
Cuts at: 332 434 577 1069 1148 1190 1517 1783 2394
Size: 102 143 492 79 42 327 266 611
Cuts at: 2394 2487 2561 2751 2813 3543 332
Size: 93 74 190 62 730 1558
Fragments arranged by size:
1558 730 611 492 327 266 190 143
102 93 79 74 62 42
BspEI T'CCGG_A
Cuts at: 3989 3989
Size: 4769
BspHI T'CATG_A
Cuts at: 3945 3945
Size: 4769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 248 1046 1658 1819 2033 2335 2716 248
Size: 798 612 161 214 302 381 2301
Fragments arranged by size:
2301 798 612 381 302 214 161
BsrBI CCG'CTC
Cuts at: 50 803 3061 3115 4599 4764 50
Size: 753 2258 54 1484 165 55
Fragments arranged by size:
2258 1484 753 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1325 1667 2681 1325
Size: 342 1014 3413
Fragments arranged by size:
3413 1014 342
BsrFI r'CCGG_y
Cuts at: 1389 1635 2767 2948 3157 1389
Size: 246 1132 181 209 3001
Fragments arranged by size:
3001 1132 246 209 181
BssHII G'CGCG_C
Cuts at: 289 289
Size: 4769
BssSI C'ACGA_G
Cuts at: 205 1189 1317 1744 3040 3398 205
Size: 984 128 427 1296 358 1576
Fragments arranged by size:
1576 1296 984 427 358 128
BstAPI GCAn_nnn'nTGC
Cuts at: 2146 2146
Size: 4769
BstBI TT'CG_AA
Cuts at: 3130 3130
Size: 4769
BstEII G'GTnAC_C
Cuts at: 633 633
Size: 4769
BstXI CCAn_nnnn'nTGG
Cuts at: 420 420
Size: 4769
BstYI r'GATC_y
Cuts at: 1 1412 1794 2261 2284 2619 2865 3877 3963
Size: 1411 382 467 23 335 246 1012 86
Cuts at: 3963 3975 4321 1
Size: 12 346 449
Fragments arranged by size:
1411 1012 467 449 382 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1124 1124
Size: 4769
BtgI C'CryG_G
Cuts at: 395 419 482 512 1013 1139 1462 395
Size: 24 63 30 501 126 323 3702
Fragments arranged by size:
3702 501 323 126 63 30 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2719 2900 3063 2719
Size: 181 163 4425
Fragments arranged by size:
4425 181 163
BtsI GCAGTG_nn'
Cuts at: 278 611 2012 278
Size: 333 1401 3035
Fragments arranged by size:
3035 1401 333
DraI TTT'AAA
Cuts at: 1768 1768
Size: 4769
DrdI GACnn_nn'nnGTC
Cuts at: 2475 3333 2475
Size: 858 3911
EaeI y'GGCC_r
Cuts at: 422 479 608 1106 1637 2354 2528 2919 2946
Size: 57 129 498 531 717 174 391 27
Cuts at: 2946 4291 422
Size: 1345 900
Fragments arranged by size:
1345 900 717 531 498 391 174 129
57 27
EagI C'GGCC_G
Cuts at: 1637 2354 1637
Size: 717 4052
EarI CTCTTCn'nnn_
Cuts at: 841 2792 3002 841
Size: 1951 210 2608
Fragments arranged by size:
2608 1951 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 319 552 567 725 1180 1195 2977 3287 3433
Size: 233 15 158 455 15 1782 310 146
Cuts at: 3433 4579 319
Size: 1146 509
Fragments arranged by size:
1782 1146 509 455 310 233 158 146
15 15
EcoICRI GAG'CTC
Cuts at: 1781 1781
Size: 4769
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 611 841 920 951 961 1117 1258 1531
Size: 478 230 79 31 10 156 141 273
Cuts at: 1531 1555 1624 1695 2594 3026 3773 4041 133
Size: 24 69 71 899 432 747 268 861
Fragments arranged by size:
899 861 747 478 432 273 268 230
156 141 79 71 69 31 24 10
EcoNI CCTnn'n_nnAGG
Cuts at: 976 1165 976
Size: 189 4580
EcoO109I rG'GnC_Cy
Cuts at: 372 525 717 1335 1575 1908 372
Size: 153 192 618 240 333 3233
Fragments arranged by size:
3233 618 333 240 192 153
EcoRI G'AATT_C
Cuts at: 1773 1773
Size: 4769
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 4769
FspI TGC'GCA
Cuts at: 604 2550 604
Size: 1946 2823
HaeII r_GCGC'y
Cuts at: 178 395 936 1197 2203 2451 3473 4452 178
Size: 217 541 261 1006 248 1022 979 495
Fragments arranged by size:
1022 1006 979 541 495 261 248 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1267 1299 2611 2641 2643 2673 1267
Size: 32 1312 30 2 30 3363
Fragments arranged by size:
3363 1312 32 30 30 2
HincII GTy'rAC
Cuts at: 1808 1808
Size: 4769
KasI G'GCGC_C
Cuts at: 174 391 2199 2447 174
Size: 217 1808 248 2496
Fragments arranged by size:
2496 1808 248 217
KpnI G_GTAC'C
Cuts at: 1789 1789
Size: 4769
MluI A'CGCG_T
Cuts at: 4202 4202
Size: 4769
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 818 3440 3624 818
Size: 2622 184 1963
Fragments arranged by size:
2622 1963 184
MscI TGG'CCA
Cuts at: 481 2530 481
Size: 2049 2720
MslI CAynn'nnrTG
Cuts at: 1276 1390 2758 2885 1276
Size: 114 1368 127 3160
Fragments arranged by size:
3160 1368 127 114
MspA1I CmG'CkG
Cuts at: 157 183 323 641 1428 1464 1476 1602 1880
Size: 26 140 318 787 36 12 126 278
Cuts at: 1880 2194 2554 3567 3812 157
Size: 314 360 1013 245 1114
Fragments arranged by size:
1114 1013 787 360 318 314 278 245
140 126 36 26 12
NaeI GCC'GGC
Cuts at: 1637 2950 1637
Size: 1313 3456
NarI GG'CG_CC
Cuts at: 175 392 2200 2448 175
Size: 217 1808 248 2496
Fragments arranged by size:
2496 1808 248 217
NcoI C'CATG_G
Cuts at: 419 482 1139 419
Size: 63 657 4049
Fragments arranged by size:
4049 657 63
NgoMIV G'CCGG_C
Cuts at: 1635 2948 1635
Size: 1313 3456
NspI r_CATG'y
Cuts at: 228 1389 1822 2322 3229 228
Size: 1161 433 500 907 1768
Fragments arranged by size:
1768 1161 907 500 433
PasI CC'CwG_GG
Cuts at: 413 570 413
Size: 157 4612
PciI A'CATG_T
Cuts at: 3225 3225
Size: 4769
PflMI CCAn_nnn'nTGG
Cuts at: 935 935
Size: 4769
PmeI GTTT'AAAC
Cuts at: 1768 1768
Size: 4769
PmlI CAC'GTG
Cuts at: 1044 1044
Size: 4769
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1117 1149 3908 3940 1117
Size: 32 2759 32 1946
Fragments arranged by size:
2759 1946 32 32
PpuMI rG'GwC_Cy
Cuts at: 717 1575 717
Size: 858 3911
PshAI GACnn'nnGTC
Cuts at: 493 1345 493
Size: 852 3917
PspXI vC'TCGA_Gb
Cuts at: 1343 1343
Size: 4769
PstI C_TGCA'G
Cuts at: 245 1076 1816 2501 245
Size: 831 740 685 2513
Fragments arranged by size:
2513 831 740 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4769
PvuII CAG'CTG
Cuts at: 1428 1476 1602 2194 2554 1428
Size: 48 126 592 360 3643
Fragments arranged by size:
3643 592 360 126 48
RsrII CG'GwC_CG
Cuts at: 2964 2964
Size: 4769
SacI G_AGCT'C
Cuts at: 1783 1783
Size: 4769
SacII CC_GC'GG
Cuts at: 1465 1465
Size: 4769
SalI G'TCGA_C
Cuts at: 1806 1806
Size: 4769
SanDI GG'GwC_CC
Cuts at: 1575 1575
Size: 4769
SapI GCTCTTCn'nnn_
Cuts at: 2792 3002 2792
Size: 210 4559
SbfI CC_TGCA'GG
Cuts at: 245 1816 245
Size: 1571 3198
ScaI AGT'ACT
Cuts at: 596 4286 596
Size: 3690 1079
SfcI C'TryA_G
Cuts at: 33 241 1072 1812 2497 3490 3681 33
Size: 208 831 740 685 993 191 1121
Fragments arranged by size:
1121 993 831 740 685 208 191
SfoI GGC'GCC
Cuts at: 176 393 2201 2449 176
Size: 217 1808 248 2496
Fragments arranged by size:
2496 1808 248 217
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4769
SmaI CCC'GGG
Cuts at: 1288 1791 1288
Size: 503 4266
SmlI C'TyrA_G
Cuts at: 1343 1924 2839 3331 3593 1343
Size: 581 915 492 262 2519
Fragments arranged by size:
2519 915 581 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4769
SphI G_CATG'C
Cuts at: 228 1822 2322 228
Size: 1594 500 2675
Fragments arranged by size:
2675 1594 500
StyI C'CwwG_G
Cuts at: 419 482 1139 1903 2257 419
Size: 63 657 764 354 2931
Fragments arranged by size:
2931 764 657 354 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2352 2485 2944 3153 2352
Size: 133 459 209 3968
Fragments arranged by size:
3968 459 209 133
TatI w'GTAC_w
Cuts at: 503 594 4284 503
Size: 91 3690 988
Fragments arranged by size:
3690 988 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 949 981 4286 4318 949
Size: 32 3305 32 1400
Fragments arranged by size:
3305 1400 32 32
Tth111I GACn'n_nGTC
Cuts at: 442 2566 442
Size: 2124 2645
XbaI T'CTAG_A
Cuts at: 1800 1800
Size: 4769
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 735 936 2264 735
Size: 201 1328 3240
Fragments arranged by size:
3240 1328 201
XhoI C'TCGA_G
Cuts at: 1343 1343
Size: 4769
XmaI C'CCGG_G
Cuts at: 1286 1789 1286
Size: 503 4266
ZraI GAC'GTC
Cuts at: 441 441
Size: 4769
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AleI AloI ApaI AscI AvrII BaeI BfrBI
BmtI BsiWI BsrGI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PsiI PspOMI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI