Restriction map for
pF1KE0027
(Circular) (Six-base) MAPSORT of: pF1KE0027.seq Check: 2410 from: 1 to: 4823
pF1KE0027 4823 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AatII G_ACGT'C
Cuts at: 254 254
Size: 4823
AccI GT'mk_AC
Cuts at: 1861 1861
Size: 4823
Acc65I G'GTAC_C
Cuts at: 1839 1839
Size: 4823
AclI AA'CG_TT
Cuts at: 4663 4663
Size: 4823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 2648 3080 3827 4095 343
Size: 2305 432 747 268 1071
Fragments arranged by size:
2305 1071 747 432 268
AflIII A'CryG_T
Cuts at: 1333 2175 3279 4256 1333
Size: 842 1104 977 1900
Fragments arranged by size:
1900 1104 977 842
AgeI A'CCGG_T
Cuts at: 3211 3211
Size: 4823
AleI CACnn'nnGTG
Cuts at: 1094 1094
Size: 4823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 212 995 1088 212
Size: 783 93 3947
Fragments arranged by size:
3947 783 93
AlwNI CAG_nnn'CTG
Cuts at: 596 798 1129 1665 2128 3695 596
Size: 202 331 536 463 1567 1724
Fragments arranged by size:
1724 1567 536 463 331 202
ApaI G_GGCC'C
Cuts at: 123 123
Size: 4823
ApaLI G'TGCA_C
Cuts at: 970 3593 970
Size: 2623 2200
ApoI r'AATT_y
Cuts at: 1827 4176 4432 1827
Size: 2349 256 2218
Fragments arranged by size:
2349 2218 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4823
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
AvaI C'yCGr_G
Cuts at: 122 231 1036 1843 122
Size: 109 805 807 3102
Fragments arranged by size:
3102 807 805 109
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 709 742 709
Size: 33 4790
BamHI G'GATC_C
Cuts at: 1848 1848
Size: 4823
BanI G'GyrC_C
Cuts at: 1839 2253 2501 2536 1839
Size: 414 248 35 4126
Fragments arranged by size:
4126 414 248 35
BanII G_rGCy'C
Cuts at: 123 230 340 362 1837 2867 123
Size: 107 110 22 1475 1030 2079
Fragments arranged by size:
2079 1475 1030 110 107 22
BbeI G_GCGC'C
Cuts at: 2257 2505 2257
Size: 248 4575
BbvCI CC'TCA_GC
Cuts at: 1360 1360
Size: 4823
BciVI GTATCCnnnnn_n'
Cuts at: 2721 3482 4823 2721
Size: 761 1341 2721
Fragments arranged by size:
2721 1341 761
BclI T'GATC_A
Cuts at: 2343 2343
Size: 4823
BfrBI ATG'CAT
Cuts at: 1432 1432
Size: 4823
BglI GCCn_nnn'nGGC
Cuts at: 1105 1105
Size: 4823
BglII A'GATC_T
Cuts at: 2338 4375 2338
Size: 2037 2786
BlpI GC'TnA_GC
Cuts at: 1186 1935 1186
Size: 749 4074
Bme1580I G_kGCm'C
Cuts at: 123 247 974 1635 2448 2541 3597 123
Size: 124 727 661 813 93 1056 1349
Fragments arranged by size:
1349 1056 813 727 661 124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1341 1451 1615 1779 2035 2116 2451 4595 1341
Size: 110 164 164 256 81 335 2144 1569
Fragments arranged by size:
2144 1569 335 256 164 164 110 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 637 3190 637
Size: 2553 2270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 703 760 1139 1229 1505 1751 519
Size: 184 57 379 90 276 246 3591
Fragments arranged by size:
3591 379 276 246 184 90 57
Bpu10I CC'TnA_GC
Cuts at: 1360 1360
Size: 4823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1539 1999 2878 3370 3668 1539
Size: 460 879 492 298 2694
Fragments arranged by size:
2694 879 492 460 298
BsaI GGTCTCn'nnnn_
Cuts at: 283 970 1676 4527 283
Size: 687 706 2851 579
Fragments arranged by size:
2851 706 687 579
BsaAI yAC'GTr
Cuts at: 762 2176 762
Size: 1414 3409
BsaBI GATnn'nnATC
Cuts at: 6 2337 4374 6
Size: 2331 2037 455
Fragments arranged by size:
2331 2037 455
BsaHI Gr'CG_yC
Cuts at: 251 2254 2502 3204 4700 251
Size: 2003 248 702 1496 374
Fragments arranged by size:
2003 1496 702 374 248
BsaWI w'CCGG_w
Cuts at: 2012 2533 3211 3485 3632 4043 2012
Size: 521 678 274 147 411 2792
Fragments arranged by size:
2792 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 624 654 1592 1622 624
Size: 30 938 30 3825
Fragments arranged by size:
3825 938 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1298 1523 1298
Size: 225 4598
BseYI C'CCAG_C
Cuts at: 201 1709 2248 3006 3583 4079 201
Size: 1508 539 758 577 496 945
Fragments arranged by size:
1508 945 758 577 539 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 488 793 1639 262
Size: 226 305 846 3446
Fragments arranged by size:
3446 846 305 226
BsiEI CG_ry'CG
Cuts at: 104 215 2365 2411 3619 104
Size: 111 2150 46 1208 1308
Fragments arranged by size:
2150 1308 1208 111 46
BsiHKAI G_wGCw'C
Cuts at: 134 340 974 1742 1837 2615 2805 3597 134
Size: 206 634 768 95 778 190 792 1360
Fragments arranged by size:
1360 792 778 768 634 206 190 95
BsiWI C'GTAC_G
Cuts at: 262 262
Size: 4823
Bsp1286I G_dGCh'C
Cuts at: 123 134 230 247 340 362 974 1635 1742
Size: 11 96 17 93 22 612 661 107
Cuts at: 1742 1837 2448 2541 2615 2805 2867 3597 123
Size: 95 611 93 74 190 62 730 1349
Fragments arranged by size:
1349 730 661 612 611 190 107 96
95 93 93 74 62 22 17 11
BspEI T'CCGG_A
Cuts at: 4043 4043
Size: 4823
BspHI T'CATG_A
Cuts at: 1490 3999 1490
Size: 2509 2314
BspMI ACCTGCnnnn'nnnn_
Cuts at: 664 815 1070 1873 2087 2389 2770 664
Size: 151 255 803 214 302 381 2717
Fragments arranged by size:
2717 803 381 302 255 214 151
BsrBI CCG'CTC
Cuts at: 50 3115 3169 4653 4818 50
Size: 3065 54 1484 165 55
Fragments arranged by size:
3065 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2735 2735
Size: 4823
BsrFI r'CCGG_y
Cuts at: 157 2821 3002 3211 157
Size: 2664 181 209 1769
Fragments arranged by size:
2664 1769 209 181
BssHII G'CGCG_C
Cuts at: 170 170
Size: 4823
BssSI C'ACGA_G
Cuts at: 967 1810 3094 3452 967
Size: 843 1284 358 2338
Fragments arranged by size:
2338 1284 843 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2200 2200
Size: 4823
BstBI TT'CG_AA
Cuts at: 3184 3184
Size: 4823
BstXI CCAn_nnnn'nTGG
Cuts at: 1722 1722
Size: 4823
BstYI r'GATC_y
Cuts at: 1 318 794 1848 2315 2338 2673 2919 3931
Size: 317 476 1054 467 23 335 246 1012
Cuts at: 3931 4017 4029 4375 1
Size: 86 12 346 449
Fragments arranged by size:
1054 1012 476 467 449 346 335 317
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1163 1294 107
Size: 1056 131 3636
Fragments arranged by size:
3636 1056 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2773 2954 3117 2773
Size: 181 163 4479
Fragments arranged by size:
4479 181 163
BtsI GCAGTG_nn'
Cuts at: 136 1089 1101 1613 2066 136
Size: 953 12 512 453 2893
Fragments arranged by size:
2893 953 512 453 12
DraI TTT'AAA
Cuts at: 1822 1822
Size: 4823
DrdI GACnn_nn'nnGTC
Cuts at: 1305 2529 3387 1305
Size: 1224 858 2741
Fragments arranged by size:
2741 1224 858
EaeI y'GGCC_r
Cuts at: 282 693 1676 2408 2582 2973 3000 4345 282
Size: 411 983 732 174 391 27 1345 760
Fragments arranged by size:
1345 983 760 732 411 391 174 27
EagI C'GGCC_G
Cuts at: 2408 2408
Size: 4823
EarI CTCTTCn'nnn_
Cuts at: 728 1555 1650 2846 3056 728
Size: 827 95 1196 210 2495
Fragments arranged by size:
2495 1196 827 210 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3031 3341 3487 4633 3031
Size: 310 146 1146 3221
Fragments arranged by size:
3221 1146 310 146
EcoICRI GAG'CTC
Cuts at: 338 1835 338
Size: 1497 3326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 519 703 760 1139 1229 1505 1751 2648
Size: 176 184 57 379 90 276 246 897
Cuts at: 2648 3080 3827 4095 343
Size: 432 747 268 1071
Fragments arranged by size:
1071 897 747 432 379 276 268 246
184 176 90 57
EcoNI CCTnn'n_nnAGG
Cuts at: 346 346
Size: 4823
EcoO109I rG'GnC_Cy
Cuts at: 119 891 1962 119
Size: 772 1071 2980
Fragments arranged by size:
2980 1071 772
EcoRI G'AATT_C
Cuts at: 1827 1827
Size: 4823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4823
FspI TGC'GCA
Cuts at: 2604 2604
Size: 4823
HaeII r_GCGC'y
Cuts at: 1710 2257 2505 3527 4506 1710
Size: 547 248 1022 979 2027
Fragments arranged by size:
2027 1022 979 547 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 296 328 805 837 1211 1243 2665 2695 2697
Size: 32 477 32 374 32 1422 30 2
Cuts at: 2697 2727 296
Size: 30 2392
Fragments arranged by size:
2392 1422 477 374 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1862 1862
Size: 4823
KasI G'GCGC_C
Cuts at: 2253 2501 2253
Size: 248 4575
KpnI G_GTAC'C
Cuts at: 1843 1843
Size: 4823
MluI A'CGCG_T
Cuts at: 4256 4256
Size: 4823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 359 3494 3678 359
Size: 3135 184 1504
Fragments arranged by size:
3135 1504 184
MscI TGG'CCA
Cuts at: 695 1678 2584 695
Size: 983 906 2934
Fragments arranged by size:
2934 983 906
MslI CAynn'nnrTG
Cuts at: 313 640 1070 1094 1621 2812 2939 313
Size: 327 430 24 527 1191 127 2197
Fragments arranged by size:
2197 1191 527 430 327 127 24
MspA1I CmG'CkG
Cuts at: 1934 2248 2608 3621 3866 1934
Size: 314 360 1013 245 2891
Fragments arranged by size:
2891 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3004 3004
Size: 4823
NarI GG'CG_CC
Cuts at: 2254 2502 2254
Size: 248 4575
NcoI C'CATG_G
Cuts at: 107 1163 1294 107
Size: 1056 131 3636
Fragments arranged by size:
3636 1056 131
NgoMIV G'CCGG_C
Cuts at: 3002 3002
Size: 4823
NsiI A_TGCA'T
Cuts at: 1434 1434
Size: 4823
NspI r_CATG'y
Cuts at: 1185 1316 1382 1436 1876 2376 3283 1185
Size: 131 66 54 440 500 907 2725
Fragments arranged by size:
2725 907 500 440 131 66 54
PasI CC'CwG_GG
Cuts at: 1402 1402
Size: 4823
PciI A'CATG_T
Cuts at: 3279 3279
Size: 4823
PflMI CCAn_nnn'nTGG
Cuts at: 1295 1353 1627 1665 1295
Size: 58 274 38 4453
Fragments arranged by size:
4453 274 58 38
PmeI GTTT'AAAC
Cuts at: 1822 1822
Size: 4823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 3962
Size: 32 4791
PspOMI G'GGCC_C
Cuts at: 119 119
Size: 4823
PstI C_TGCA'G
Cuts at: 143 1096 1525 1564 1766 1870 2555 143
Size: 953 429 39 202 104 685 2411
Fragments arranged by size:
2411 953 685 429 202 104 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4823
PvuII CAG'CTG
Cuts at: 2248 2608 2248
Size: 360 4463
RsrII CG'GwC_CG
Cuts at: 3018 3018
Size: 4823
SacI G_AGCT'C
Cuts at: 340 1837 340
Size: 1497 3326
SalI G'TCGA_C
Cuts at: 1860 1860
Size: 4823
SapI GCTCTTCn'nnn_
Cuts at: 728 2846 3056 728
Size: 2118 210 2495
Fragments arranged by size:
2495 2118 210
SbfI CC_TGCA'GG
Cuts at: 1525 1870 1525
Size: 345 4478
ScaI AGT'ACT
Cuts at: 4340 4340
Size: 4823
SexAI A'CCwGG_T
Cuts at: 900 900
Size: 4823
SfcI C'TryA_G
Cuts at: 33 139 363 575 1092 1109 1521 1560 1762
Size: 106 224 212 517 17 412 39 202
Cuts at: 1762 1866 2551 3544 3735 33
Size: 104 685 993 191 1121
Fragments arranged by size:
1121 993 685 517 412 224 212 202
191 106 104 39 17
SfoI GGC'GCC
Cuts at: 2255 2503 2255
Size: 248 4575
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
SmaI CCC'GGG
Cuts at: 124 233 1845 124
Size: 109 1612 3102
Fragments arranged by size:
3102 1612 109
SmlI C'TyrA_G
Cuts at: 1554 1978 2893 3385 3647 1554
Size: 424 915 492 262 2730
Fragments arranged by size:
2730 915 492 424 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4823
SphI G_CATG'C
Cuts at: 1382 1876 2376 1382
Size: 494 500 3829
Fragments arranged by size:
3829 500 494
SspI AAT'ATT
Cuts at: 865 865
Size: 4823
StyI C'CwwG_G
Cuts at: 107 221 696 1010 1163 1238 1294 1595 1673
Size: 114 475 314 153 75 56 301 78
Cuts at: 1673 1957 2311 107
Size: 284 354 2619
Fragments arranged by size:
2619 475 354 314 301 284 153 114
78 75 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 229 1003 2406 2539 2998 3207 229
Size: 774 1403 133 459 209 1845
Fragments arranged by size:
1845 1403 774 459 209 133
TatI w'GTAC_w
Cuts at: 653 4338 653
Size: 3685 1138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1592 1624 4340 4372 1592
Size: 32 2716 32 2043
Fragments arranged by size:
2716 2043 32 32
Tth111I GACn'n_nGTC
Cuts at: 1123 2620 1123
Size: 1497 3326
XbaI T'CTAG_A
Cuts at: 1854 1854
Size: 4823
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1170 2318 1170
Size: 1148 3675
XmaI C'CCGG_G
Cuts at: 122 231 1843 122
Size: 109 1612 3102
Fragments arranged by size:
3102 1612 109
ZraI GAC'GTC
Cuts at: 252 252
Size: 4823
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI AscI AvrII BbsI
BcgI BmgBI BmtI BsmI BsmBI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI