Restriction map for
pF1KE0017
(Circular) (Six-base) MAPSORT of: pF1KE0017.seq Check: 2469 from: 1 to: 4763
pF1KE0017 4763 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1801 1801
Size: 4763
Acc65I G'GTAC_C
Cuts at: 1779 1779
Size: 4763
AclI AA'CG_TT
Cuts at: 1092 4603 1092
Size: 3511 1252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 635 1202 2588 3020 3767 4035 243
Size: 392 567 1386 432 747 268 971
Fragments arranged by size:
1386 971 747 567 432 392 268
AflIII A'CryG_T
Cuts at: 1514 2115 3219 4196 1514
Size: 601 1104 977 2081
Fragments arranged by size:
2081 1104 977 601
AgeI A'CCGG_T
Cuts at: 3151 3151
Size: 4763
AhdI GACnn_n'nnGTC
Cuts at: 444 444
Size: 4763
AleI CACnn'nnGTG
Cuts at: 1342 1342
Size: 4763
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1344 1344
Size: 4763
AlwNI CAG_nnn'CTG
Cuts at: 1051 1573 2068 3635 1051
Size: 522 495 1567 2179
Fragments arranged by size:
2179 1567 522 495
ApaLI G'TGCA_C
Cuts at: 3533 3533
Size: 4763
ApoI r'AATT_y
Cuts at: 357 1767 4116 4372 357
Size: 1410 2349 256 748
Fragments arranged by size:
2349 1410 748 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4763
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
AvaI C'yCGr_G
Cuts at: 1783 1783
Size: 4763
AvrII C'CTAG_G
Cuts at: 529 529
Size: 4763
BamHI G'GATC_C
Cuts at: 1788 1788
Size: 4763
BanI G'GyrC_C
Cuts at: 1779 2193 2441 2476 1779
Size: 414 248 35 4066
Fragments arranged by size:
4066 414 248 35
BanII G_rGCy'C
Cuts at: 139 1777 2807 139
Size: 1638 1030 2095
Fragments arranged by size:
2095 1638 1030
BbeI G_GCGC'C
Cuts at: 2197 2445 2197
Size: 248 4515
BbsI GAAGACnn'nnnn_
Cuts at: 146 281 464 722 146
Size: 135 183 258 4187
Fragments arranged by size:
4187 258 183 135
BciVI GTATCCnnnnn_n'
Cuts at: 2661 3422 4763 2661
Size: 761 1341 2661
Fragments arranged by size:
2661 1341 761
BclI T'GATC_A
Cuts at: 1254 2283 1254
Size: 1029 3734
BglI GCCn_nnn'nGGC
Cuts at: 1657 1657
Size: 4763
BglII A'GATC_T
Cuts at: 2278 4315 2278
Size: 2037 2726
BlpI GC'TnA_GC
Cuts at: 1875 1875
Size: 4763
Bme1580I G_kGCm'C
Cuts at: 1722 2388 2481 3537 1722
Size: 666 93 1056 2948
Fragments arranged by size:
2948 1056 666 93
BmrI ACTGGGnnnn_n'
Cuts at: 133 1384 1975 2056 2391 4535 133
Size: 1251 591 81 335 2144 361
Fragments arranged by size:
2144 1251 591 361 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 4763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1172 1172
Size: 4763
Bpu10I CC'TnA_GC
Cuts at: 1241 1241
Size: 4763
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 917 1939 2818 3310 3608 856
Size: 61 1022 879 492 298 2011
Fragments arranged by size:
2011 1022 879 492 298 61
BsaI GGTCTCn'nnnn_
Cuts at: 1530 1693 4467 1530
Size: 163 2774 1826
Fragments arranged by size:
2774 1826 163
BsaAI yAC'GTr
Cuts at: 2116 2116
Size: 4763
BsaBI GATnn'nnATC
Cuts at: 6 1010 2277 4314 6
Size: 1004 1267 2037 455
Fragments arranged by size:
2037 1267 1004 455
BsaHI Gr'CG_yC
Cuts at: 490 2194 2442 3144 4640 490
Size: 1704 248 702 1496 613
Fragments arranged by size:
1704 1496 702 613 248
BsaWI w'CCGG_w
Cuts at: 1952 2473 3151 3425 3572 3983 1952
Size: 521 678 274 147 411 2732
Fragments arranged by size:
2732 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1441 2188 2946 3523 4019 1441
Size: 747 758 577 496 2185
Fragments arranged by size:
2185 758 747 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1539 1539
Size: 4763
BsiEI CG_ry'CG
Cuts at: 104 2305 2351 3559 104
Size: 2201 46 1208 1308
Fragments arranged by size:
2201 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1777 2555 2745 3537 1777
Size: 778 190 792 3003
Fragments arranged by size:
3003 792 778 190
BsmI GAATG_Cn'
Cuts at: 319 970 1063 319
Size: 651 93 4019
Fragments arranged by size:
4019 651 93
Bsp1286I G_dGCh'C
Cuts at: 139 1722 1777 2388 2481 2555 2745 2807 3537
Size: 1583 55 611 93 74 190 62 730
Cuts at: 3537 139
Size: 1365
Fragments arranged by size:
1583 1365 730 611 190 93 74 62
55
BspEI T'CCGG_A
Cuts at: 3983 3983
Size: 4763
BspHI T'CATG_A
Cuts at: 165 3939 165
Size: 3774 989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1813 2027 2329 2710 1813
Size: 214 302 381 3866
Fragments arranged by size:
3866 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3055 3109 4593 4758 50
Size: 3005 54 1484 165 55
Fragments arranged by size:
3005 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 924 1452 1562 2675 924
Size: 528 110 1113 3012
Fragments arranged by size:
3012 1113 528 110
BsrFI r'CCGG_y
Cuts at: 1235 2761 2942 3151 1235
Size: 1526 181 209 2847
Fragments arranged by size:
2847 1526 209 181
BssSI C'ACGA_G
Cuts at: 3034 3392 3034
Size: 358 4405
BstAPI GCAn_nnn'nTGC
Cuts at: 2140 2140
Size: 4763
BstBI TT'CG_AA
Cuts at: 3124 3124
Size: 4763
BstXI CCAn_nnnn'nTGG
Cuts at: 1150 1150
Size: 4763
BstYI r'GATC_y
Cuts at: 1 1788 2255 2278 2613 2859 3871 3957 3969
Size: 1787 467 23 335 246 1012 86 12
Cuts at: 3969 4315 1
Size: 346 449
Fragments arranged by size:
1787 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1389 107
Size: 1282 3481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2713 2894 3057 2713
Size: 181 163 4419
Fragments arranged by size:
4419 181 163
BtsI GCAGTG_nn'
Cuts at: 179 804 1283 1527 2006 179
Size: 625 479 244 479 2936
Fragments arranged by size:
2936 625 479 479 244
DraI TTT'AAA
Cuts at: 1762 1762
Size: 4763
DraIII CAC_nnn'GTG
Cuts at: 1390 1390
Size: 4763
DrdI GACnn_nn'nnGTC
Cuts at: 2469 3327 2469
Size: 858 3905
EaeI y'GGCC_r
Cuts at: 2348 2522 2913 2940 4285 2348
Size: 174 391 27 1345 2826
Fragments arranged by size:
2826 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2348 2348
Size: 4763
EarI CTCTTCn'nnn_
Cuts at: 224 1418 1520 2786 2996 224
Size: 1194 102 1266 210 1991
Fragments arranged by size:
1991 1266 1194 210 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2971 3281 3427 4573 127
Size: 2844 310 146 1146 317
Fragments arranged by size:
2844 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1775 1775
Size: 4763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 635 1172 1202 2588 3020 3767 4035 243
Size: 392 537 30 1386 432 747 268 971
Fragments arranged by size:
1386 971 747 537 432 392 268 30
EcoO109I rG'GnC_Cy
Cuts at: 1902 1902
Size: 4763
EcoRI G'AATT_C
Cuts at: 1767 1767
Size: 4763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4763
FspI TGC'GCA
Cuts at: 2544 2544
Size: 4763
HaeII r_GCGC'y
Cuts at: 2197 2445 3467 4446 2197
Size: 248 1022 979 2514
Fragments arranged by size:
2514 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 430 462 556 588 2605 2635 2637 2667 430
Size: 32 94 32 2017 30 2 30 2526
Fragments arranged by size:
2526 2017 94 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1802 1802
Size: 4763
HindIII A'AGCT_T
Cuts at: 972 1193 972
Size: 221 4542
KasI G'GCGC_C
Cuts at: 2193 2441 2193
Size: 248 4515
KpnI G_GTAC'C
Cuts at: 1783 1783
Size: 4763
MluI A'CGCG_T
Cuts at: 4196 4196
Size: 4763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3434 3618 3434
Size: 184 4579
MscI TGG'CCA
Cuts at: 2524 2524
Size: 4763
MslI CAynn'nnrTG
Cuts at: 203 1342 2752 2879 203
Size: 1139 1410 127 2087
Fragments arranged by size:
2087 1410 1139 127
MspA1I CmG'CkG
Cuts at: 1874 2188 2548 3561 3806 1874
Size: 314 360 1013 245 2831
Fragments arranged by size:
2831 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2944 2944
Size: 4763
NarI GG'CG_CC
Cuts at: 2194 2442 2194
Size: 248 4515
NcoI C'CATG_G
Cuts at: 107 107
Size: 4763
NgoMIV G'CCGG_C
Cuts at: 2942 2942
Size: 4763
NspI r_CATG'y
Cuts at: 557 985 1518 1816 2316 3223 557
Size: 428 533 298 500 907 2097
Fragments arranged by size:
2097 907 533 500 428 298
PciI A'CATG_T
Cuts at: 1514 3219 1514
Size: 1705 3058
PmeI GTTT'AAAC
Cuts at: 1762 1762
Size: 4763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 285 317 971 1003 3902 3934 285
Size: 32 654 32 2899 32 1114
Fragments arranged by size:
2899 1114 654 32 32 32
PstI C_TGCA'G
Cuts at: 828 1810 2495 828
Size: 982 685 3096
Fragments arranged by size:
3096 982 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4763
PvuII CAG'CTG
Cuts at: 2188 2548 2188
Size: 360 4403
RsrII CG'GwC_CG
Cuts at: 2958 2958
Size: 4763
SacI G_AGCT'C
Cuts at: 1777 1777
Size: 4763
SalI G'TCGA_C
Cuts at: 1800 1800
Size: 4763
SapI GCTCTTCn'nnn_
Cuts at: 2786 2996 2786
Size: 210 4553
SbfI CC_TGCA'GG
Cuts at: 1810 1810
Size: 4763
ScaI AGT'ACT
Cuts at: 4280 4280
Size: 4763
SfcI C'TryA_G
Cuts at: 33 824 1806 2491 3484 3675 33
Size: 791 982 685 993 191 1121
Fragments arranged by size:
1121 993 982 791 685 191
SfoI GGC'GCC
Cuts at: 2195 2443 2195
Size: 248 4515
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
SmaI CCC'GGG
Cuts at: 1785 1785
Size: 4763
SmlI C'TyrA_G
Cuts at: 871 932 1918 2833 3325 3587 871
Size: 61 986 915 492 262 2047
Fragments arranged by size:
2047 986 915 492 262 61
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4763
SphI G_CATG'C
Cuts at: 1816 2316 1816
Size: 500 4263
StuI AGG'CCT
Cuts at: 534 928 534
Size: 394 4369
StyI C'CwwG_G
Cuts at: 107 529 1897 2251 107
Size: 422 1368 354 2619
Fragments arranged by size:
2619 1368 422 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1189 1600 2346 2479 2938 3147 1189
Size: 411 746 133 459 209 2805
Fragments arranged by size:
2805 746 459 411 209 133
TatI w'GTAC_w
Cuts at: 4278 4278
Size: 4763
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 783 815 4280 4312 783
Size: 32 3465 32 1234
Fragments arranged by size:
3465 1234 32 32
Tth111I GACn'n_nGTC
Cuts at: 2560 2560
Size: 4763
XbaI T'CTAG_A
Cuts at: 303 1794 303
Size: 1491 3272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1653 2258 1653
Size: 605 4158
XmaI C'CCGG_G
Cuts at: 1783 1783
Size: 4763
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsaXI BseRI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI