Restriction map for
pF1KE0011
(Circular) (Six-base) MAPSORT of: pF1KE0011.seq Check: 1893 from: 1 to: 5552
pF1KE0011 5552 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 1865 1865
Size: 5552
AccI GT'mk_AC
Cuts at: 493 2590 493
Size: 2097 3455
Acc65I G'GTAC_C
Cuts at: 2568 2568
Size: 5552
AclI AA'CG_TT
Cuts at: 5392 5392
Size: 5552
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 1147 1786 2023 2080 2440 3377 3809 4556
Size: 392 639 237 57 360 937 432 747
Cuts at: 4556 4824 755
Size: 268 1483
Fragments arranged by size:
1483 937 747 639 432 392 360 268
237 57
AfeI AGC'GCT
Cuts at: 1391 1391
Size: 5552
AflIII A'CryG_T
Cuts at: 2904 4008 4985 2904
Size: 1104 977 3471
Fragments arranged by size:
3471 1104 977
AgeI A'CCGG_T
Cuts at: 3940 3940
Size: 5552
AlwNI CAG_nnn'CTG
Cuts at: 1764 1890 2857 4424 1764
Size: 126 967 1567 2892
Fragments arranged by size:
2892 1567 967 126
ApaLI G'TGCA_C
Cuts at: 2044 4322 2044
Size: 2278 3274
ApoI r'AATT_y
Cuts at: 2556 4905 5161 2556
Size: 2349 256 2947
Fragments arranged by size:
2947 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5552
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5552
AvaI C'yCGr_G
Cuts at: 1871 2572 1871
Size: 701 4851
BamHI G'GATC_C
Cuts at: 2577 2577
Size: 5552
BanI G'GyrC_C
Cuts at: 361 2568 2982 3230 3265 361
Size: 2207 414 248 35 2648
Fragments arranged by size:
2648 2207 414 248 35
BanII G_rGCy'C
Cuts at: 314 335 1105 1566 1925 2566 3596 314
Size: 21 770 461 359 641 1030 2270
Fragments arranged by size:
2270 1030 770 641 461 359 21
BbeI G_GCGC'C
Cuts at: 2986 3234 2986
Size: 248 5304
BbsI GAAGACnn'nnnn_
Cuts at: 1585 1757 1585
Size: 172 5380
BbvCI CC'TCA_GC
Cuts at: 772 1393 2104 772
Size: 621 711 4220
Fragments arranged by size:
4220 711 621
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1363 1397 1824 1858 1363
Size: 34 427 34 5057
Fragments arranged by size:
5057 427 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 731 1137 3450 4211 5552 731
Size: 406 2313 761 1341 731
Fragments arranged by size:
2313 1341 761 731 406
BclI T'GATC_A
Cuts at: 3072 3072
Size: 5552
BglII A'GATC_T
Cuts at: 3067 5104 3067
Size: 2037 3515
BlpI GC'TnA_GC
Cuts at: 994 2008 2664 994
Size: 1014 656 3882
Fragments arranged by size:
3882 1014 656
Bme1580I G_kGCm'C
Cuts at: 2048 3177 3270 4326 2048
Size: 1129 93 1056 3274
Fragments arranged by size:
3274 1129 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 995 2764 2845 3180 5324 995
Size: 1769 81 335 2144 1223
Fragments arranged by size:
2144 1769 1223 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2532 3919 2532
Size: 1387 4165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 586 921 1538 1843 1996 2017 2502 160
Size: 426 335 617 305 153 21 485 3210
Fragments arranged by size:
3210 617 485 426 335 305 153 21
Bpu10I CC'TnA_GC
Cuts at: 772 1393 2104 772
Size: 621 711 4220
Fragments arranged by size:
4220 711 621
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 1771 2467 2728 3607 4099 4397 1540
Size: 231 696 261 879 492 298 2695
Fragments arranged by size:
2695 879 696 492 298 261 231
BsaI GGTCTCn'nnnn_
Cuts at: 749 1514 2458 5256 749
Size: 765 944 2798 1045
Fragments arranged by size:
2798 1045 944 765
BsaAI yAC'GTr
Cuts at: 2905 2905
Size: 5552
BsaBI GATnn'nnATC
Cuts at: 6 3066 5103 6
Size: 3060 2037 455
Fragments arranged by size:
3060 2037 455
BsaHI Gr'CG_yC
Cuts at: 1862 2983 3231 3933 5429 1862
Size: 1121 248 702 1496 1985
Fragments arranged by size:
1985 1496 1121 702 248
BsaWI w'CCGG_w
Cuts at: 830 878 2741 3262 3940 4214 4361 4772 830
Size: 48 1863 521 678 274 147 411 1610
Fragments arranged by size:
1863 1610 678 521 411 274 147 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 140 170 140
Size: 30 5522
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 703 897 1432 1954 2089 2521 2524 703
Size: 194 535 522 135 432 3 3731
Fragments arranged by size:
3731 535 522 432 194 135 3
BseYI C'CCAG_C
Cuts at: 766 979 1961 2166 2977 3735 4312 4808 766
Size: 213 982 205 811 758 577 496 1510
Fragments arranged by size:
1510 982 811 758 577 496 213 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 828 940 1684 2220 828
Size: 112 744 536 4160
Fragments arranged by size:
4160 744 536 112
BsiEI CG_ry'CG
Cuts at: 104 3094 3140 4348 104
Size: 2990 46 1208 1308
Fragments arranged by size:
2990 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 314 335 662 1000 1286 1566 1925 2014 2048
Size: 21 327 338 286 280 359 89 34
Cuts at: 2048 2057 2566 3344 3534 4326 314
Size: 9 509 778 190 792 1540
Fragments arranged by size:
1540 792 778 509 359 338 327 286
280 190 89 34 21 9
BsmI GAATG_Cn'
Cuts at: 855 1596 855
Size: 741 4811
BsmBI CGTCTCn'nnnn_
Cuts at: 178 178
Size: 5552
Bsp1286I G_dGCh'C
Cuts at: 314 335 662 1000 1105 1286 1566 1925 2014
Size: 21 327 338 105 181 280 359 89
Cuts at: 2014 2048 2057 2566 3177 3270 3344 3534 3596
Size: 34 9 509 611 93 74 190 62
Cuts at: 3596 4326 314
Size: 730 1540
Fragments arranged by size:
1540 730 611 509 359 338 327 280
190 181 105 93 89 74 62 34
21 9
BspEI T'CCGG_A
Cuts at: 878 4772 878
Size: 3894 1658
BspHI T'CATG_A
Cuts at: 248 1566 1610 4728 248
Size: 1318 44 3118 1072
Fragments arranged by size:
3118 1318 1072 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2602 2816 3118 3499 2602
Size: 214 302 381 4655
Fragments arranged by size:
4655 381 302 214
BsrBI CCG'CTC
Cuts at: 50 867 1495 3844 3898 5382 5547 50
Size: 817 628 2349 54 1484 165 55
Fragments arranged by size:
2349 1484 817 628 165 55 54
BsrDI GCAATG_nn'
Cuts at: 323 1020 1344 3464 323
Size: 697 324 2120 2411
Fragments arranged by size:
2411 2120 697 324
BsrFI r'CCGG_y
Cuts at: 872 1651 3550 3731 3940 872
Size: 779 1899 181 209 2484
Fragments arranged by size:
2484 1899 779 209 181
BssSI C'ACGA_G
Cuts at: 424 3823 4181 424
Size: 3399 358 1795
Fragments arranged by size:
3399 1795 358
BstAPI GCAn_nnn'nTGC
Cuts at: 552 2094 2929 552
Size: 1542 835 3175
Fragments arranged by size:
3175 1542 835
BstBI TT'CG_AA
Cuts at: 3913 3913
Size: 5552
BstXI CCAn_nnnn'nTGG
Cuts at: 1137 1320 1137
Size: 183 5369
BstYI r'GATC_y
Cuts at: 1 1213 1893 2577 3044 3067 3402 3648 4660
Size: 1212 680 684 467 23 335 246 1012
Cuts at: 4660 4746 4758 5104 1
Size: 86 12 346 449
Fragments arranged by size:
1212 1012 684 680 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 497 611 1646 2447 107
Size: 390 114 1035 801 3212
Fragments arranged by size:
3212 1035 801 390 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1011 3502 3683 3846 1011
Size: 2491 181 163 2717
Fragments arranged by size:
2717 2491 181 163
BtsI GCAGTG_nn'
Cuts at: 531 1140 1426 1913 2316 2795 531
Size: 609 286 487 403 479 3288
Fragments arranged by size:
3288 609 487 479 403 286
ClaI AT'CG_AT
Cuts at: 1844 1844
Size: 5552
DraI TTT'AAA
Cuts at: 2551 2551
Size: 5552
DrdI GACnn_nn'nnGTC
Cuts at: 3258 4116 3258
Size: 858 4694
EaeI y'GGCC_r
Cuts at: 218 614 708 945 1090 1649 1928 1946 2444
Size: 396 94 237 145 559 279 18 498
Cuts at: 2444 3137 3311 3702 3729 5074 218
Size: 693 174 391 27 1345 696
Fragments arranged by size:
1345 696 693 559 498 396 391 279
237 174 145 94 27 18
EagI C'GGCC_G
Cuts at: 3137 3137
Size: 5552
EarI CTCTTCn'nnn_
Cuts at: 586 731 773 857 3575 3785 586
Size: 145 42 84 2718 210 2353
Fragments arranged by size:
2718 2353 210 145 84 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3760 4070 4216 5362 3760
Size: 310 146 1146 3950
Fragments arranged by size:
3950 1146 310 146
EcoICRI GAG'CTC
Cuts at: 312 333 1564 1923 2564 312
Size: 21 1231 359 641 3300
Fragments arranged by size:
3300 1231 641 359 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 586 755 921 1147 1538 1786 1843 1996
Size: 426 169 166 226 391 248 57 153
Cuts at: 1996 2017 2023 2080 2440 2502 3377 3809 4556
Size: 21 6 57 360 62 875 432 747
Cuts at: 4556 4824 160
Size: 268 888
Fragments arranged by size:
888 875 747 432 426 391 360 268
248 226 169 166 153 62 57 57
21 6
EcoO109I rG'GnC_Cy
Cuts at: 198 714 931 1508 1992 2691 198
Size: 516 217 577 484 699 3059
Fragments arranged by size:
3059 699 577 516 484 217
EcoRI G'AATT_C
Cuts at: 2556 2556
Size: 5552
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3571 3571
Size: 5552
FspI TGC'GCA
Cuts at: 3333 3333
Size: 5552
HaeII r_GCGC'y
Cuts at: 1393 1453 2986 3234 4256 5235 1393
Size: 60 1533 248 1022 979 1710
Fragments arranged by size:
1710 1533 1022 979 248 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 336 368 373 392 405 424 451 483 520
Size: 32 5 19 13 19 27 32 37
Cuts at: 520 552 1205 1237 1878 1910 2146 2178 3394
Size: 32 653 32 641 32 236 32 1216
Cuts at: 3394 3424 3426 3456 336
Size: 30 2 30 2432
Fragments arranged by size:
2432 1216 653 641 236 37 32 32
32 32 32 32 30 30 27 19
19 13 5 2
HincII GTy'rAC
Cuts at: 432 2591 432
Size: 2159 3393
KasI G'GCGC_C
Cuts at: 2982 3230 2982
Size: 248 5304
KpnI G_GTAC'C
Cuts at: 2572 2572
Size: 5552
MluI A'CGCG_T
Cuts at: 4985 4985
Size: 5552
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1712 2343 4223 4407 1712
Size: 631 1880 184 2857
Fragments arranged by size:
2857 1880 631 184
MscI TGG'CCA
Cuts at: 220 1930 2446 3313 220
Size: 1710 516 867 2459
Fragments arranged by size:
2459 1710 867 516
MslI CAynn'nnrTG
Cuts at: 263 1360 1549 1930 2531 3541 3668 263
Size: 1097 189 381 601 1010 127 2147
Fragments arranged by size:
2147 1097 1010 601 381 189 127
MspA1I CmG'CkG
Cuts at: 599 1585 1628 2091 2120 2663 2977 3337 4350
Size: 986 43 463 29 543 314 360 1013
Cuts at: 4350 4595 599
Size: 245 1556
Fragments arranged by size:
1556 1013 986 543 463 360 314 245
43 29
NaeI GCC'GGC
Cuts at: 1653 3733 1653
Size: 2080 3472
NarI GG'CG_CC
Cuts at: 2983 3231 2983
Size: 248 5304
NcoI C'CATG_G
Cuts at: 107 497 2447 107
Size: 390 1950 3212
Fragments arranged by size:
3212 1950 390
NgoMIV G'CCGG_C
Cuts at: 1651 3731 1651
Size: 2080 3472
NspI r_CATG'y
Cuts at: 1359 2605 3105 4012 1359
Size: 1246 500 907 2899
Fragments arranged by size:
2899 1246 907 500
PasI CC'CwG_GG
Cuts at: 1670 1670
Size: 5552
PciI A'CATG_T
Cuts at: 4008 4008
Size: 5552
PflMI CCAn_nnn'nTGG
Cuts at: 1890 1890
Size: 5552
PfoI T'CCnGG_A
Cuts at: 269 857 269
Size: 588 4964
PmeI GTTT'AAAC
Cuts at: 2551 2551
Size: 5552
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 4691
Size: 32 5520
PpuMI rG'GwC_Cy
Cuts at: 198 714 931 1508 198
Size: 516 217 577 4242
Fragments arranged by size:
4242 577 516 217
PshAI GACnn'nnGTC
Cuts at: 469 469
Size: 5552
PstI C_TGCA'G
Cuts at: 526 548 604 1660 2337 2599 3284 526
Size: 22 56 1056 677 262 685 2794
Fragments arranged by size:
2794 1056 685 677 262 56 22
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5552
PvuII CAG'CTG
Cuts at: 599 1585 1628 2977 3337 599
Size: 986 43 1349 360 2814
Fragments arranged by size:
2814 1349 986 360 43
RsrII CG'GwC_CG
Cuts at: 3747 3747
Size: 5552
SacI G_AGCT'C
Cuts at: 314 335 1566 1925 2566 314
Size: 21 1231 359 641 3300
Fragments arranged by size:
3300 1231 641 359 21
SalI G'TCGA_C
Cuts at: 2589 2589
Size: 5552
SapI GCTCTTCn'nnn_
Cuts at: 586 857 3575 3785 586
Size: 271 2718 210 2353
Fragments arranged by size:
2718 2353 271 210
SbfI CC_TGCA'GG
Cuts at: 2599 2599
Size: 5552
ScaI AGT'ACT
Cuts at: 388 5069 388
Size: 4681 871
SexAI A'CCwGG_T
Cuts at: 2039 2372 2039
Size: 333 5219
SfcI C'TryA_G
Cuts at: 33 522 544 600 910 1656 2333 2595 3280
Size: 489 22 56 310 746 677 262 685
Cuts at: 3280 4273 4464 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 746 685 677 489 310 262
191 56 22
SfoI GGC'GCC
Cuts at: 2984 3232 2984
Size: 248 5304
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5552
SmaI CCC'GGG
Cuts at: 2574 2574
Size: 5552
SmlI C'TyrA_G
Cuts at: 1555 1786 2482 2707 3622 4114 4376 1555
Size: 231 696 225 915 492 262 2731
Fragments arranged by size:
2731 915 696 492 262 231 225
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5552
SphI G_CATG'C
Cuts at: 1359 2605 3105 1359
Size: 1246 500 3806
Fragments arranged by size:
3806 1246 500
StuI AGG'CCT
Cuts at: 1603 2035 1603
Size: 432 5120
StyI C'CwwG_G
Cuts at: 107 221 497 1319 1598 2288 2447 2686 3040
Size: 114 276 822 279 690 159 239 354
Cuts at: 3040 107
Size: 2619
Fragments arranged by size:
2619 822 690 354 279 276 239 159
114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3135 3268 3727 3936 3135
Size: 133 459 209 4751
Fragments arranged by size:
4751 459 209 133
TatI w'GTAC_w
Cuts at: 386 1409 5067 386
Size: 1023 3658 871
Fragments arranged by size:
3658 1023 871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 140 172 1276 1308 2047 2079 5069 5101 140
Size: 32 1104 32 739 32 2990 32 591
Fragments arranged by size:
2990 1104 739 591 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1065 3349 1065
Size: 2284 3268
XbaI T'CTAG_A
Cuts at: 2583 2583
Size: 5552
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1824 2370 3047 1824
Size: 546 677 4329
Fragments arranged by size:
4329 677 546
XmaI C'CCGG_G
Cuts at: 2572 2572
Size: 5552
ZraI GAC'GTC
Cuts at: 1863 1863
Size: 5552
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI BsrGI
BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI