Restriction map for
pF1KE0001
(Circular) (Six-base) MAPSORT of: pF1KE0001.seq Check: 2537 from: 1 to: 4604
pF1KE0001 4604 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 652 652
Size: 4604
AccI GT'mk_AC
Cuts at: 689 1642 689
Size: 953 3651
Acc65I G'GTAC_C
Cuts at: 1022 1620 1022
Size: 598 4006
AclI AA'CG_TT
Cuts at: 4444 4444
Size: 4604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 2429 2861 3608 3876 902
Size: 1527 432 747 268 1630
Fragments arranged by size:
1630 1527 747 432 268
AflIII A'CryG_T
Cuts at: 1012 1956 3060 4037 1012
Size: 944 1104 977 1579
Fragments arranged by size:
1579 1104 977 944
AgeI A'CCGG_T
Cuts at: 2992 2992
Size: 4604
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1344 1344
Size: 4604
AlwNI CAG_nnn'CTG
Cuts at: 447 1909 3476 447
Size: 1462 1567 1575
Fragments arranged by size:
1575 1567 1462
ApaI G_GGCC'C
Cuts at: 416 416
Size: 4604
ApaLI G'TGCA_C
Cuts at: 582 1438 3374 582
Size: 856 1936 1812
Fragments arranged by size:
1936 1812 856
ApoI r'AATT_y
Cuts at: 1608 3957 4213 1608
Size: 2349 256 1999
Fragments arranged by size:
2349 1999 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4604
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
AvaI C'yCGr_G
Cuts at: 242 534 570 1559 1624 242
Size: 292 36 989 65 3222
Fragments arranged by size:
3222 989 292 65 36
BamHI G'GATC_C
Cuts at: 163 1124 1629 163
Size: 961 505 3138
Fragments arranged by size:
3138 961 505
BanI G'GyrC_C
Cuts at: 1022 1620 2034 2282 2317 1022
Size: 598 414 248 35 3309
Fragments arranged by size:
3309 598 414 248 35
BanII G_rGCy'C
Cuts at: 416 1383 1519 1618 2648 416
Size: 967 136 99 1030 2372
Fragments arranged by size:
2372 1030 967 136 99
BbeI G_GCGC'C
Cuts at: 2038 2286 2038
Size: 248 4356
BbsI GAAGACnn'nnnn_
Cuts at: 1510 1510
Size: 4604
BbvCI CC'TCA_GC
Cuts at: 1216 1216
Size: 4604
BciVI GTATCCnnnnn_n'
Cuts at: 2502 3263 4604 2502
Size: 761 1341 2502
Fragments arranged by size:
2502 1341 761
BclI T'GATC_A
Cuts at: 2124 2124
Size: 4604
BglI GCCn_nnn'nGGC
Cuts at: 1037 1037
Size: 4604
BglII A'GATC_T
Cuts at: 1061 2119 4156 1061
Size: 1058 2037 1509
Fragments arranged by size:
2037 1509 1058
BlpI GC'TnA_GC
Cuts at: 211 1295 1716 211
Size: 1084 421 3099
Fragments arranged by size:
3099 1084 421
Bme1580I G_kGCm'C
Cuts at: 416 586 772 1033 1306 1442 2229 2322 3378
Size: 170 186 261 273 136 787 93 1056
Cuts at: 3378 416
Size: 1642
Fragments arranged by size:
1642 1056 787 273 261 186 170 136
93
BmgBI CAC'GTC
Cuts at: 1476 1482 1476
Size: 6 4598
BmrI ACTGGGnnnn_n'
Cuts at: 468 1386 1816 1897 2232 4376 468
Size: 918 430 81 335 2144 696
Fragments arranged by size:
2144 918 696 430 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 255 585 2971 255
Size: 330 2386 1888
Fragments arranged by size:
2386 1888 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 385
Size: 4604
Bpu10I CC'TnA_GC
Cuts at: 1216 1522 1216
Size: 306 4298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1401 1780 2659 3151 3449 1401
Size: 379 879 492 298 2556
Fragments arranged by size:
2556 879 492 379 298
BsaI GGTCTCn'nnnn_
Cuts at: 912 4308 912
Size: 3396 1208
BsaAI yAC'GTr
Cuts at: 1013 1512 1957 1013
Size: 499 445 3660
Fragments arranged by size:
3660 499 445
BsaBI GATnn'nnATC
Cuts at: 6 2118 4155 6
Size: 2112 2037 455
Fragments arranged by size:
2112 2037 455
BsaHI Gr'CG_yC
Cuts at: 2035 2283 2985 4481 2035
Size: 248 702 1496 2158
Fragments arranged by size:
2158 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1383 1577 1793 2314 2992 3266 3413 3824 1383
Size: 194 216 521 678 274 147 411 2163
Fragments arranged by size:
2163 678 521 411 274 216 194 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 813 843 1133 1163 813
Size: 30 290 30 4254
Fragments arranged by size:
4254 290 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 292 1547 217
Size: 75 1255 3274
Fragments arranged by size:
3274 1255 75
BseYI C'CCAG_C
Cuts at: 775 1032 2029 2787 3364 3860 775
Size: 257 997 758 577 496 1519
Fragments arranged by size:
1519 997 758 577 496 257
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 337 511 1228 1285 277
Size: 60 174 717 57 3596
Fragments arranged by size:
3596 717 174 60 57
BsiEI CG_ry'CG
Cuts at: 104 1289 2146 2192 3400 104
Size: 1185 857 46 1208 1308
Fragments arranged by size:
1308 1208 1185 857 46
BsiHKAI G_wGCw'C
Cuts at: 410 571 586 1442 1618 2396 2586 3378 410
Size: 161 15 856 176 778 190 792 1636
Fragments arranged by size:
1636 856 792 778 190 176 161 15
Bsp1286I G_dGCh'C
Cuts at: 410 416 571 586 772 1033 1306 1383 1442
Size: 6 155 15 186 261 273 77 59
Cuts at: 1442 1519 1618 2229 2322 2396 2586 2648 3378
Size: 77 99 611 93 74 190 62 730
Cuts at: 3378 410
Size: 1636
Fragments arranged by size:
1636 730 611 273 261 190 186 155
99 93 77 77 74 62 59 15
6
BspEI T'CCGG_A
Cuts at: 1577 3824 1577
Size: 2247 2357
BspHI T'CATG_A
Cuts at: 3780 3780
Size: 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 455 652 1587 1654 1868 2170 2551 455
Size: 197 935 67 214 302 381 2508
Fragments arranged by size:
2508 935 381 302 214 197 67
BsrBI CCG'CTC
Cuts at: 50 2896 2950 4434 4599 50
Size: 2846 54 1484 165 55
Fragments arranged by size:
2846 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2516 2516
Size: 4604
BsrFI r'CCGG_y
Cuts at: 752 2602 2783 2992 752
Size: 1850 181 209 2364
Fragments arranged by size:
2364 1850 209 181
BssSI C'ACGA_G
Cuts at: 2875 3233 2875
Size: 358 4246
BstAPI GCAn_nnn'nTGC
Cuts at: 1981 1981
Size: 4604
BstBI TT'CG_AA
Cuts at: 2965 2965
Size: 4604
BstXI CCAn_nnnn'nTGG
Cuts at: 426 426
Size: 4604
BstYI r'GATC_y
Cuts at: 1 163 281 1061 1124 1580 1629 2096 2119
Size: 162 118 780 63 456 49 467 23
Cuts at: 2119 2454 2700 3712 3798 3810 4156 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 780 467 456 449 346 335 246
162 118 86 63 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 323 323
Size: 4604
BtgI C'CryG_G
Cuts at: 1490 1490
Size: 4604
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2554 2735 2898 2554
Size: 181 163 4260
Fragments arranged by size:
4260 181 163
BtsI GCAGTG_nn'
Cuts at: 1326 1353 1847 1326
Size: 27 494 4083
Fragments arranged by size:
4083 494 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1446 1481 1446
Size: 35 4569
DraI TTT'AAA
Cuts at: 1603 1603
Size: 4604
DraIII CAC_nnn'GTG
Cuts at: 1206 1206
Size: 4604
DrdI GACnn_nn'nnGTC
Cuts at: 2310 3168 2310
Size: 858 3746
EaeI y'GGCC_r
Cuts at: 750 2189 2363 2754 2781 4126 750
Size: 1439 174 391 27 1345 1228
Fragments arranged by size:
1439 1345 1228 391 174 27
EagI C'GGCC_G
Cuts at: 2189 2189
Size: 4604
EarI CTCTTCn'nnn_
Cuts at: 2627 2837 2627
Size: 210 4394
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2812 3122 3268 4414 2812
Size: 310 146 1146 3002
Fragments arranged by size:
3002 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1616 1616
Size: 4604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 902 2429 2861 3608 3876 385
Size: 517 1527 432 747 268 1113
Fragments arranged by size:
1527 1113 747 517 432 268
EcoO109I rG'GnC_Cy
Cuts at: 574 1173 1743 574
Size: 599 570 3435
Fragments arranged by size:
3435 599 570
EcoRI G'AATT_C
Cuts at: 1608 1608
Size: 4604
EcoRV GAT'ATC
Cuts at: 1155 1155
Size: 4604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1194 2623 1194
Size: 1429 3175
FseI GG_CCGG'CC
Cuts at: 756 756
Size: 4604
FspI TGC'GCA
Cuts at: 2385 2385
Size: 4604
HaeII r_GCGC'y
Cuts at: 455 2038 2286 3308 4287 455
Size: 1583 248 1022 979 772
Fragments arranged by size:
1583 1022 979 772 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1278 1310 2446 2476 2478 2508 1278
Size: 32 1136 30 2 30 3374
Fragments arranged by size:
3374 1136 32 30 30 2
HincII GTy'rAC
Cuts at: 951 1643 951
Size: 692 3912
HindIII A'AGCT_T
Cuts at: 301 301
Size: 4604
KasI G'GCGC_C
Cuts at: 2034 2282 2034
Size: 248 4356
KpnI G_GTAC'C
Cuts at: 1026 1624 1026
Size: 598 4006
MluI A'CGCG_T
Cuts at: 4037 4037
Size: 4604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 356 927 3275 3459 356
Size: 571 2348 184 1501
Fragments arranged by size:
2348 1501 571 184
MscI TGG'CCA
Cuts at: 2365 2365
Size: 4604
MslI CAynn'nnrTG
Cuts at: 985 2593 2720 985
Size: 1608 127 2869
Fragments arranged by size:
2869 1608 127
MspA1I CmG'CkG
Cuts at: 791 1299 1715 2029 2389 3402 3647 791
Size: 508 416 314 360 1013 245 1748
Fragments arranged by size:
1748 1013 508 416 360 314 245
NaeI GCC'GGC
Cuts at: 754 2785 754
Size: 2031 2573
NarI GG'CG_CC
Cuts at: 2035 2283 2035
Size: 248 4356
NdeI CA'TA_TG
Cuts at: 1099 1099
Size: 4604
NgoMIV G'CCGG_C
Cuts at: 752 2783 752
Size: 2031 2573
NspI r_CATG'y
Cuts at: 1657 2157 3064 1657
Size: 500 907 3197
Fragments arranged by size:
3197 907 500
PasI CC'CwG_GG
Cuts at: 263 263
Size: 4604
PciI A'CATG_T
Cuts at: 3060 3060
Size: 4604
PfoI T'CCnGG_A
Cuts at: 1127 1127
Size: 4604
PmeI GTTT'AAAC
Cuts at: 1603 1603
Size: 4604
PmlI CAC'GTG
Cuts at: 1013 1013
Size: 4604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 3743
Size: 32 4572
PpuMI rG'GwC_Cy
Cuts at: 1173 1173
Size: 4604
PshAI GACnn'nnGTC
Cuts at: 964 964
Size: 4604
PspOMI G'GGCC_C
Cuts at: 412 412
Size: 4604
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 673 705 673
Size: 32 4572
PstI C_TGCA'G
Cuts at: 381 796 1651 2336 381
Size: 415 855 685 2649
Fragments arranged by size:
2649 855 685 415
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4604
PvuII CAG'CTG
Cuts at: 791 1299 2029 2389 791
Size: 508 730 360 3006
Fragments arranged by size:
3006 730 508 360
RsrII CG'GwC_CG
Cuts at: 2799 2799
Size: 4604
SacI G_AGCT'C
Cuts at: 1618 1618
Size: 4604
SalI G'TCGA_C
Cuts at: 1641 1641
Size: 4604
SapI GCTCTTCn'nnn_
Cuts at: 2627 2837 2627
Size: 210 4394
SbfI CC_TGCA'GG
Cuts at: 1651 1651
Size: 4604
ScaI AGT'ACT
Cuts at: 4121 4121
Size: 4604
SfcI C'TryA_G
Cuts at: 33 377 792 1647 2332 3325 3516 33
Size: 344 415 855 685 993 191 1121
Fragments arranged by size:
1121 993 855 685 415 344 191
SfoI GGC'GCC
Cuts at: 2036 2284 2036
Size: 248 4356
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
SmaI CCC'GGG
Cuts at: 244 1626 244
Size: 1382 3222
SmlI C'TyrA_G
Cuts at: 1416 1759 2674 3166 3428 1416
Size: 343 915 492 262 2592
Fragments arranged by size:
2592 915 492 343 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4604
SphI G_CATG'C
Cuts at: 1657 2157 1657
Size: 500 4104
StuI AGG'CCT
Cuts at: 328 1145 1573 328
Size: 817 428 3359
Fragments arranged by size:
3359 817 428
StyI C'CwwG_G
Cuts at: 137 168 1738 2092 137
Size: 31 1570 354 2649
Fragments arranged by size:
2649 1570 354 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 112 789 2187 2320 2779 2988 112
Size: 677 1398 133 459 209 1728
Fragments arranged by size:
1728 1398 677 459 209 133
TatI w'GTAC_w
Cuts at: 341 4119 341
Size: 3778 826
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1133 1165 4121 4153 1133
Size: 32 2956 32 1584
Fragments arranged by size:
2956 1584 32 32
Tth111I GACn'n_nGTC
Cuts at: 2401 2401
Size: 4604
XbaI T'CTAG_A
Cuts at: 1635 1635
Size: 4604
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 423 719 1242 2099 423
Size: 296 523 857 2928
Fragments arranged by size:
2928 857 523 296
XmaI C'CCGG_G
Cuts at: 242 1624 242
Size: 1382 3222
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI EcoNI FspAI HpaI MfeI
NcoI NheI NotI NruI NsiI PacI PflMI PsiI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI