Restriction map for
pF1KB9995
(Circular) (Six-base) MAPSORT of: pF1KB9995.seq Check: 8581 from: 1 to: 4868
pF1KB9995 4868 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1906 1906
Size: 4868
Acc65I G'GTAC_C
Cuts at: 1884 1884
Size: 4868
AclI AA'CG_TT
Cuts at: 4708 4708
Size: 4868
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 2693 3125 3872 4140 448
Size: 2245 432 747 268 1176
Fragments arranged by size:
2245 1176 747 432 268
AflIII A'CryG_T
Cuts at: 374 716 848 2220 3324 4301 374
Size: 342 132 1372 1104 977 941
Fragments arranged by size:
1372 1104 977 941 342 132
AgeI A'CCGG_T
Cuts at: 3256 3256
Size: 4868
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1214 1337 1384 1214
Size: 123 47 4698
Fragments arranged by size:
4698 123 47
AlwNI CAG_nnn'CTG
Cuts at: 2173 3740 2173
Size: 1567 3301
ApaLI G'TGCA_C
Cuts at: 3638 3638
Size: 4868
ApoI r'AATT_y
Cuts at: 570 581 1872 4221 4477 570
Size: 11 1291 2349 256 961
Fragments arranged by size:
2349 1291 961 256 11
AseI AT'TA_AT
Cuts at: 20 20
Size: 4868
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
AvaI C'yCGr_G
Cuts at: 1888 1888
Size: 4868
BamHI G'GATC_C
Cuts at: 1893 1893
Size: 4868
BanI G'GyrC_C
Cuts at: 194 835 1884 2298 2546 2581 194
Size: 641 1049 414 248 35 2481
Fragments arranged by size:
2481 1049 641 414 248 35
BanII G_rGCy'C
Cuts at: 124 808 1882 2912 124
Size: 684 1074 1030 2080
Fragments arranged by size:
2080 1074 1030 684
BbeI G_GCGC'C
Cuts at: 839 2302 2550 839
Size: 1463 248 3157
Fragments arranged by size:
3157 1463 248
BbsI GAAGACnn'nnnn_
Cuts at: 1664 1664
Size: 4868
BbvCI CC'TCA_GC
Cuts at: 1604 1604
Size: 4868
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 176 210 176
Size: 34 4834
BciVI GTATCCnnnnn_n'
Cuts at: 2766 3527 4868 2766
Size: 761 1341 2766
Fragments arranged by size:
2766 1341 761
BclI T'GATC_A
Cuts at: 2388 2388
Size: 4868
BfrBI ATG'CAT
Cuts at: 591 1191 1371 591
Size: 600 180 4088
Fragments arranged by size:
4088 600 180
BglI GCCn_nnn'nGGC
Cuts at: 316 316
Size: 4868
BglII A'GATC_T
Cuts at: 1181 2383 4420 1181
Size: 1202 2037 1629
Fragments arranged by size:
2037 1629 1202
BlpI GC'TnA_GC
Cuts at: 1980 1980
Size: 4868
Bme1580I G_kGCm'C
Cuts at: 2493 2586 3642 2493
Size: 93 1056 3719
Fragments arranged by size:
3719 1056 93
BmgBI CAC'GTC
Cuts at: 938 1280 938
Size: 342 4526
BmrI ACTGGGnnnn_n'
Cuts at: 2080 2161 2496 4640 2080
Size: 81 335 2144 2308
Fragments arranged by size:
2308 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3235 3235
Size: 4868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 1445 1682 722
Size: 723 237 3908
Fragments arranged by size:
3908 723 237
Bpu10I CC'TnA_GC
Cuts at: 1604 1604
Size: 4868
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 2044 2923 3415 3713 946
Size: 1098 879 492 298 2101
Fragments arranged by size:
2101 1098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4572 4572
Size: 4868
BsaAI yAC'GTr
Cuts at: 2221 2221
Size: 4868
BsaBI GATnn'nnATC
Cuts at: 6 2382 4419 6
Size: 2376 2037 455
Fragments arranged by size:
2376 2037 455
BsaHI Gr'CG_yC
Cuts at: 260 836 1449 2299 2547 3249 4745 260
Size: 576 613 850 248 702 1496 383
Fragments arranged by size:
1496 850 702 613 576 383 248
BsaWI w'CCGG_w
Cuts at: 2057 2578 3256 3530 3677 4088 2057
Size: 521 678 274 147 411 2837
Fragments arranged by size:
2837 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1751 1781 1751
Size: 30 4838
BseYI C'CCAG_C
Cuts at: 1797 2293 3051 3628 4124 1797
Size: 496 758 577 496 2541
Fragments arranged by size:
2541 758 577 496 496
BsiEI CG_ry'CG
Cuts at: 104 2410 2456 3664 104
Size: 2306 46 1208 1308
Fragments arranged by size:
2306 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1321 1882 2660 2850 3642 1321
Size: 561 778 190 792 2547
Fragments arranged by size:
2547 792 778 561 190
BsmBI CGTCTCn'nnnn_
Cuts at: 251 251
Size: 4868
Bsp1286I G_dGCh'C
Cuts at: 124 808 1321 1882 2493 2586 2660 2850 2912
Size: 684 513 561 611 93 74 190 62
Cuts at: 2912 3642 124
Size: 730 1350
Fragments arranged by size:
1350 730 684 611 561 513 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4088 4088
Size: 4868
BspHI T'CATG_A
Cuts at: 4044 4044
Size: 4868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 404 1918 2132 2434 2815 404
Size: 1514 214 302 381 2457
Fragments arranged by size:
2457 1514 381 302 214
BsrBI CCG'CTC
Cuts at: 50 281 3160 3214 4698 4863 50
Size: 231 2879 54 1484 165 55
Fragments arranged by size:
2879 1484 231 165 55 54
BsrDI GCAATG_nn'
Cuts at: 591 926 2780 591
Size: 335 1854 2679
Fragments arranged by size:
2679 1854 335
BsrFI r'CCGG_y
Cuts at: 270 402 2866 3047 3256 270
Size: 132 2464 181 209 1882
Fragments arranged by size:
2464 1882 209 181 132
BssSI C'ACGA_G
Cuts at: 1099 3139 3497 1099
Size: 2040 358 2470
Fragments arranged by size:
2470 2040 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2245 2245
Size: 4868
BstBI TT'CG_AA
Cuts at: 3229 3229
Size: 4868
BstXI CCAn_nnnn'nTGG
Cuts at: 1719 1719
Size: 4868
BstYI r'GATC_y
Cuts at: 1 1181 1893 2360 2383 2718 2964 3976 4062
Size: 1180 712 467 23 335 246 1012 86
Cuts at: 4062 4074 4420 1
Size: 12 346 449
Fragments arranged by size:
1180 1012 712 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 981 981
Size: 4868
BtgI C'CryG_G
Cuts at: 107 298 107
Size: 191 4677
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 827 2818 2999 3162 827
Size: 1991 181 163 2533
Fragments arranged by size:
2533 1991 181 163
BtsI GCAGTG_nn'
Cuts at: 1321 2111 1321
Size: 790 4078
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 554 589 554
Size: 35 4833
DraI TTT'AAA
Cuts at: 1467 1867 1467
Size: 400 4468
DrdI GACnn_nn'nnGTC
Cuts at: 2574 3432 2574
Size: 858 4010
EaeI y'GGCC_r
Cuts at: 796 2453 2627 3018 3045 4390 796
Size: 1657 174 391 27 1345 1274
Fragments arranged by size:
1657 1345 1274 391 174 27
EagI C'GGCC_G
Cuts at: 2453 2453
Size: 4868
EarI CTCTTCn'nnn_
Cuts at: 920 1334 1661 2891 3101 920
Size: 414 327 1230 210 2687
Fragments arranged by size:
2687 1230 414 327 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 289 3076 3386 3532 4678 289
Size: 2787 310 146 1146 479
Fragments arranged by size:
2787 1146 479 310 146
EcoICRI GAG'CTC
Cuts at: 1880 1880
Size: 4868
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 722 1445 1682 2693 3125 3872 4140 448
Size: 274 723 237 1011 432 747 268 1176
Fragments arranged by size:
1176 1011 747 723 432 274 268 237
EcoNI CCTnn'n_nnAGG
Cuts at: 1687 1687
Size: 4868
EcoO109I rG'GnC_Cy
Cuts at: 2007 2007
Size: 4868
EcoRI G'AATT_C
Cuts at: 1872 1872
Size: 4868
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 443 2887 443
Size: 2444 2424
FspI TGC'GCA
Cuts at: 2649 2649
Size: 4868
HaeII r_GCGC'y
Cuts at: 839 2302 2550 3572 4551 839
Size: 1463 248 1022 979 1156
Fragments arranged by size:
1463 1156 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 810 842 1269 1301 1662 1694 1751 1783 2710
Size: 32 427 32 361 32 57 32 927
Cuts at: 2710 2740 2742 2772 810
Size: 30 2 30 2906
Fragments arranged by size:
2906 927 427 361 57 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 369 1907 369
Size: 1538 3330
HindIII A'AGCT_T
Cuts at: 1641 1641
Size: 4868
KasI G'GCGC_C
Cuts at: 835 2298 2546 835
Size: 1463 248 3157
Fragments arranged by size:
3157 1463 248
KpnI G_GTAC'C
Cuts at: 1888 1888
Size: 4868
MluI A'CGCG_T
Cuts at: 374 4301 374
Size: 3927 941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 125 654 3539 3723 125
Size: 529 2885 184 1270
Fragments arranged by size:
2885 1270 529 184
MscI TGG'CCA
Cuts at: 798 2629 798
Size: 1831 3037
MslI CAynn'nnrTG
Cuts at: 1196 1457 1613 2857 2984 1196
Size: 261 156 1244 127 3080
Fragments arranged by size:
3080 1244 261 156 127
MspA1I CmG'CkG
Cuts at: 300 350 1114 1801 1979 2293 2653 3666 3911
Size: 50 764 687 178 314 360 1013 245
Cuts at: 3911 300
Size: 1257
Fragments arranged by size:
1257 1013 764 687 360 314 245 178
50
NaeI GCC'GGC
Cuts at: 272 404 3049 272
Size: 132 2645 2091
Fragments arranged by size:
2645 2091 132
NarI GG'CG_CC
Cuts at: 836 2299 2547 836
Size: 1463 248 3157
Fragments arranged by size:
3157 1463 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4868
NgoMIV G'CCGG_C
Cuts at: 270 402 3047 270
Size: 132 2645 2091
Fragments arranged by size:
2645 2091 132
NsiI A_TGCA'T
Cuts at: 593 1193 1373 593
Size: 600 180 4088
Fragments arranged by size:
4088 600 180
NspI r_CATG'y
Cuts at: 720 852 1195 1375 1519 1921 2421 3328 720
Size: 132 343 180 144 402 500 907 2260
Fragments arranged by size:
2260 907 500 402 343 180 144 132
PciI A'CATG_T
Cuts at: 716 848 3324 716
Size: 132 2476 2260
Fragments arranged by size:
2476 2260 132
PflMI CCAn_nnn'nTGG
Cuts at: 1755 1755
Size: 4868
PmeI GTTT'AAAC
Cuts at: 1867 1867
Size: 4868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 4007
Size: 32 4836
PstI C_TGCA'G
Cuts at: 349 978 1915 2600 349
Size: 629 937 685 2617
Fragments arranged by size:
2617 937 685 629
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4868
PvuII CAG'CTG
Cuts at: 350 1114 2293 2653 350
Size: 764 1179 360 2565
Fragments arranged by size:
2565 1179 764 360
RsrII CG'GwC_CG
Cuts at: 3063 3063
Size: 4868
SacI G_AGCT'C
Cuts at: 1882 1882
Size: 4868
SacII CC_GC'GG
Cuts at: 301 301
Size: 4868
SalI G'TCGA_C
Cuts at: 1905 1905
Size: 4868
SapI GCTCTTCn'nnn_
Cuts at: 920 2891 3101 920
Size: 1971 210 2687
Fragments arranged by size:
2687 1971 210
SbfI CC_TGCA'GG
Cuts at: 1915 1915
Size: 4868
ScaI AGT'ACT
Cuts at: 4385 4385
Size: 4868
SfcI C'TryA_G
Cuts at: 33 345 974 1911 2596 3589 3780 33
Size: 312 629 937 685 993 191 1121
Fragments arranged by size:
1121 993 937 685 629 312 191
SfoI GGC'GCC
Cuts at: 837 2300 2548 837
Size: 1463 248 3157
Fragments arranged by size:
3157 1463 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
SmaI CCC'GGG
Cuts at: 1890 1890
Size: 4868
SmlI C'TyrA_G
Cuts at: 961 2023 2938 3430 3692 961
Size: 1062 915 492 262 2137
Fragments arranged by size:
2137 1062 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4868
SphI G_CATG'C
Cuts at: 1375 1921 2421 1375
Size: 546 500 3822
Fragments arranged by size:
3822 546 500
StuI AGG'CCT
Cuts at: 1435 1435
Size: 4868
StyI C'CwwG_G
Cuts at: 107 1754 1848 2002 2356 107
Size: 1647 94 154 354 2619
Fragments arranged by size:
2619 1647 354 154 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2451 2584 3043 3252 2451
Size: 133 459 209 4067
Fragments arranged by size:
4067 459 209 133
TatI w'GTAC_w
Cuts at: 4383 4383
Size: 4868
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1109 1141 1236 1268 4385 4417 1109
Size: 32 95 32 3117 32 1560
Fragments arranged by size:
3117 1560 95 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1765 2665 1765
Size: 900 3968
XbaI T'CTAG_A
Cuts at: 1899 1899
Size: 4868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2363 2363
Size: 4868
XmaI C'CCGG_G
Cuts at: 1888 1888
Size: 4868
XmnI GAAnn'nnTTC
Cuts at: 481 481
Size: 4868
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BmtI BseRI BsgI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI