Restriction map for
pF1KB9987
(Circular) (Six-base) MAPSORT of: pF1KB9987.seq Check: 560 from: 1 to: 4835
pF1KB9987 4835 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 246 385 246
Size: 139 4696
AccI GT'mk_AC
Cuts at: 1721 1873 1721
Size: 152 4683
Acc65I G'GTAC_C
Cuts at: 1851 1851
Size: 4835
AclI AA'CG_TT
Cuts at: 4675 4675
Size: 4835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1690 1798 2660 3092 3839 4107 1690
Size: 108 862 432 747 268 2418
Fragments arranged by size:
2418 862 747 432 268 108
AfeI AGC'GCT
Cuts at: 1480 1480
Size: 4835
AflIII A'CryG_T
Cuts at: 2187 3291 4268 2187
Size: 1104 977 2754
Fragments arranged by size:
2754 1104 977
AgeI A'CCGG_T
Cuts at: 3223 3223
Size: 4835
AleI CACnn'nnGTG
Cuts at: 793 793
Size: 4835
AlwNI CAG_nnn'CTG
Cuts at: 192 419 885 1029 1094 1264 2140 3707 192
Size: 227 466 144 65 170 876 1567 1320
Fragments arranged by size:
1567 1320 876 466 227 170 144 65
ApaLI G'TGCA_C
Cuts at: 875 3605 875
Size: 2730 2105
ApoI r'AATT_y
Cuts at: 1384 1644 1839 4188 4444 1384
Size: 260 195 2349 256 1775
Fragments arranged by size:
2349 1775 260 256 195
AseI AT'TA_AT
Cuts at: 20 20
Size: 4835
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
AvaI C'yCGr_G
Cuts at: 980 1855 980
Size: 875 3960
BamHI G'GATC_C
Cuts at: 1860 1860
Size: 4835
BanI G'GyrC_C
Cuts at: 261 957 1151 1851 2265 2513 2548 261
Size: 696 194 700 414 248 35 2548
Fragments arranged by size:
2548 700 696 414 248 194 35
BanII G_rGCy'C
Cuts at: 507 1849 2879 507
Size: 1342 1030 2463
Fragments arranged by size:
2463 1342 1030
BbeI G_GCGC'C
Cuts at: 2269 2517 2269
Size: 248 4587
BbsI GAAGACnn'nnnn_
Cuts at: 416 416
Size: 4835
BbvCI CC'TCA_GC
Cuts at: 116 633 116
Size: 517 4318
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4801
BciVI GTATCCnnnnn_n'
Cuts at: 372 875 2733 3494 4835 372
Size: 503 1858 761 1341 372
Fragments arranged by size:
1858 1341 761 503 372
BclI T'GATC_A
Cuts at: 2355 2355
Size: 4835
BglI GCCn_nnn'nGGC
Cuts at: 1701 1701
Size: 4835
BglII A'GATC_T
Cuts at: 2350 4387 2350
Size: 2037 2798
BlpI GC'TnA_GC
Cuts at: 1947 1947
Size: 4835
Bme1580I G_kGCm'C
Cuts at: 879 1219 1697 2460 2553 3609 879
Size: 340 478 763 93 1056 2105
Fragments arranged by size:
2105 1056 763 478 340 93
BmgBI CAC'GTC
Cuts at: 1542 1542
Size: 4835
BmrI ACTGGGnnnn_n'
Cuts at: 2047 2128 2463 4607 2047
Size: 81 335 2144 2275
Fragments arranged by size:
2275 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3202 3202
Size: 4835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1055 1274 418
Size: 637 219 3979
Fragments arranged by size:
3979 637 219
Bpu10I CC'TnA_GC
Cuts at: 116 633 116
Size: 517 4318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 374 2011 2890 3382 3680 302
Size: 72 1637 879 492 298 1457
Fragments arranged by size:
1637 1457 879 492 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 1224 4539 1224
Size: 3315 1520
BsaAI yAC'GTr
Cuts at: 2188 2188
Size: 4835
BsaBI GATnn'nnATC
Cuts at: 6 1507 2349 4386 6
Size: 1501 842 2037 455
Fragments arranged by size:
2037 1501 842 455
BsaHI Gr'CG_yC
Cuts at: 2266 2514 3216 4712 2266
Size: 248 702 1496 2389
Fragments arranged by size:
2389 1496 702 248
BsaWI w'CCGG_w
Cuts at: 304 2024 2545 3223 3497 3644 4055 304
Size: 1720 521 678 274 147 411 1084
Fragments arranged by size:
1720 1084 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 286 316 1028 1058 286
Size: 30 712 30 4063
Fragments arranged by size:
4063 712 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 104 1778 104
Size: 1674 3161
BseYI C'CCAG_C
Cuts at: 677 737 1087 1244 1257 2260 3018 3595 4091
Size: 60 350 157 13 1003 758 577 496
Cuts at: 4091 677
Size: 1421
Fragments arranged by size:
1421 1003 758 577 496 350 157 60
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 1039 1084 263
Size: 776 45 4014
Fragments arranged by size:
4014 776 45
BsiEI CG_ry'CG
Cuts at: 104 2377 2423 3631 104
Size: 2273 46 1208 1308
Fragments arranged by size:
2273 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 466 879 1197 1667 1849 2627 2817 3609 466
Size: 413 318 470 182 778 190 792 1692
Fragments arranged by size:
1692 792 778 470 413 318 190 182
BsmI GAATG_Cn'
Cuts at: 146 146
Size: 4835
Bsp1286I G_dGCh'C
Cuts at: 466 507 879 1197 1219 1667 1697 1849 2460
Size: 41 372 318 22 448 30 152 611
Cuts at: 2460 2553 2627 2817 2879 3609 466
Size: 93 74 190 62 730 1692
Fragments arranged by size:
1692 730 611 448 372 318 190 152
93 74 62 41 30 22
BspEI T'CCGG_A
Cuts at: 304 4055 304
Size: 3751 1084
BspHI T'CATG_A
Cuts at: 4011 4011
Size: 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 246 360 385 1885 2099 2401 2782 246
Size: 114 25 1500 214 302 381 2299
Fragments arranged by size:
2299 1500 381 302 214 114 25
BsrBI CCG'CTC
Cuts at: 50 3127 3181 4665 4830 50
Size: 3077 54 1484 165 55
Fragments arranged by size:
3077 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2747 2747
Size: 4835
BsrFI r'CCGG_y
Cuts at: 1008 2833 3014 3223 1008
Size: 1825 181 209 2620
Fragments arranged by size:
2620 1825 209 181
BssSI C'ACGA_G
Cuts at: 3106 3464 3106
Size: 358 4477
BstAPI GCAn_nnn'nTGC
Cuts at: 1029 1062 2212 1029
Size: 33 1150 3652
Fragments arranged by size:
3652 1150 33
BstBI TT'CG_AA
Cuts at: 3196 3196
Size: 4835
BstEII G'GTnAC_C
Cuts at: 372 372
Size: 4835
BstXI CCAn_nnnn'nTGG
Cuts at: 937 937
Size: 4835
BstYI r'GATC_y
Cuts at: 1 705 1860 2327 2350 2685 2931 3943 4029
Size: 704 1155 467 23 335 246 1012 86
Cuts at: 4029 4041 4387 1
Size: 12 346 449
Fragments arranged by size:
1155 1012 704 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1486 1486
Size: 4835
BtgI C'CryG_G
Cuts at: 913 1792 913
Size: 879 3956
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2785 2966 3129 2785
Size: 181 163 4491
Fragments arranged by size:
4491 181 163
BtsI GCAGTG_nn'
Cuts at: 2078 2078
Size: 4835
DraI TTT'AAA
Cuts at: 1834 1834
Size: 4835
DraIII CAC_nnn'GTG
Cuts at: 957 1062 957
Size: 105 4730
DrdI GACnn_nn'nnGTC
Cuts at: 2541 3399 2541
Size: 858 3977
EaeI y'GGCC_r
Cuts at: 591 966 2420 2594 2985 3012 4357 591
Size: 375 1454 174 391 27 1345 1069
Fragments arranged by size:
1454 1345 1069 391 375 174 27
EagI C'GGCC_G
Cuts at: 2420 2420
Size: 4835
EarI CTCTTCn'nnn_
Cuts at: 215 1100 1672 1769 2858 3068 215
Size: 885 572 97 1089 210 1982
Fragments arranged by size:
1982 1089 885 572 210 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3043 3353 3499 4645 3043
Size: 310 146 1146 3233
Fragments arranged by size:
3233 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1847 1847
Size: 4835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1055 1274 1690 1798 2660 3092 3839 4107
Size: 637 219 416 108 862 432 747 268
Cuts at: 4107 418
Size: 1146
Fragments arranged by size:
1146 862 747 637 432 416 268 219
108
EcoO109I rG'GnC_Cy
Cuts at: 776 810 1798 1974 776
Size: 34 988 176 3637
Fragments arranged by size:
3637 988 176 34
EcoRI G'AATT_C
Cuts at: 1839 1839
Size: 4835
EcoRV GAT'ATC
Cuts at: 1461 1461
Size: 4835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4835
FspI TGC'GCA
Cuts at: 385 2616 385
Size: 2231 2604
HaeII r_GCGC'y
Cuts at: 1482 1553 2269 2517 3539 4518 1482
Size: 71 716 248 1022 979 1799
Fragments arranged by size:
1799 1022 979 716 248 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 469 501 873 905 2677 2707 2709 2739 469
Size: 32 372 32 1772 30 2 30 2565
Fragments arranged by size:
2565 1772 372 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1874 1874
Size: 4835
HindIII A'AGCT_T
Cuts at: 446 446
Size: 4835
KasI G'GCGC_C
Cuts at: 2265 2513 2265
Size: 248 4587
KpnI G_GTAC'C
Cuts at: 1855 1855
Size: 4835
MfeI C'AATT_G
Cuts at: 1424 1424
Size: 4835
MluI A'CGCG_T
Cuts at: 4268 4268
Size: 4835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 480 3506 3690 480
Size: 3026 184 1625
Fragments arranged by size:
3026 1625 184
MscI TGG'CCA
Cuts at: 593 968 2596 593
Size: 375 1628 2832
Fragments arranged by size:
2832 1628 375
MslI CAynn'nnrTG
Cuts at: 151 793 1369 1660 2824 2951 151
Size: 642 576 291 1164 127 2035
Fragments arranged by size:
2035 1164 642 576 291 127
MspA1I CmG'CkG
Cuts at: 354 574 768 1261 1563 1727 1946 2260 2620
Size: 220 194 493 302 164 219 314 360
Cuts at: 2620 3633 3878 354
Size: 1013 245 1311
Fragments arranged by size:
1311 1013 493 360 314 302 245 220
219 194 164
NaeI GCC'GGC
Cuts at: 1010 3016 1010
Size: 2006 2829
NarI GG'CG_CC
Cuts at: 2266 2514 2266
Size: 248 4587
NcoI C'CATG_G
Cuts at: 913 913
Size: 4835
NdeI CA'TA_TG
Cuts at: 963 963
Size: 4835
NgoMIV G'CCGG_C
Cuts at: 1008 3014 1008
Size: 2006 2829
NspI r_CATG'y
Cuts at: 875 910 1693 1888 2388 3295 875
Size: 35 783 195 500 907 2415
Fragments arranged by size:
2415 907 783 500 195 35
PasI CC'CwG_GG
Cuts at: 1094 1094
Size: 4835
PciI A'CATG_T
Cuts at: 3291 3291
Size: 4835
PflMI CCAn_nnn'nTGG
Cuts at: 663 1094 663
Size: 431 4404
PfoI T'CCnGG_A
Cuts at: 936 936
Size: 4835
PmeI GTTT'AAAC
Cuts at: 1834 1834
Size: 4835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 3974
Size: 32 4803
PpuMI rG'GwC_Cy
Cuts at: 810 810
Size: 4835
PspXI vC'TCGA_Gb
Cuts at: 980 980
Size: 4835
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 910 942 910
Size: 32 4803
PstI C_TGCA'G
Cuts at: 424 471 516 1224 1882 2567 424
Size: 47 45 708 658 685 2692
Fragments arranged by size:
2692 708 685 658 47 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4835
PvuII CAG'CTG
Cuts at: 768 1261 1563 1727 2260 2620 768
Size: 493 302 164 533 360 2983
Fragments arranged by size:
2983 533 493 360 302 164
RsrII CG'GwC_CG
Cuts at: 3030 3030
Size: 4835
SacI G_AGCT'C
Cuts at: 1849 1849
Size: 4835
SalI G'TCGA_C
Cuts at: 1872 1872
Size: 4835
SapI GCTCTTCn'nnn_
Cuts at: 1672 2858 3068 1672
Size: 1186 210 3439
Fragments arranged by size:
3439 1186 210
SbfI CC_TGCA'GG
Cuts at: 1882 1882
Size: 4835
ScaI AGT'ACT
Cuts at: 4352 4352
Size: 4835
SexAI A'CCwGG_T
Cuts at: 953 953
Size: 4835
SfcI C'TryA_G
Cuts at: 33 420 467 512 1220 1722 1878 2563 3556
Size: 387 47 45 708 502 156 685 993
Cuts at: 3556 3747 33
Size: 191 1121
Fragments arranged by size:
1121 993 708 685 502 387 191 156
47 45
SfoI GGC'GCC
Cuts at: 2267 2515 2267
Size: 248 4587
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
SmaI CCC'GGG
Cuts at: 1857 1857
Size: 4835
SmlI C'TyrA_G
Cuts at: 317 389 980 1990 2905 3397 3659 317
Size: 72 591 1010 915 492 262 1493
Fragments arranged by size:
1493 1010 915 591 492 262 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4835
SphI G_CATG'C
Cuts at: 1888 2388 1888
Size: 500 4335
SspI AAT'ATT
Cuts at: 211 211
Size: 4835
StuI AGG'CCT
Cuts at: 437 701 1052 437
Size: 264 351 4220
Fragments arranged by size:
4220 351 264
StyI C'CwwG_G
Cuts at: 644 913 1147 1208 1826 1969 2323 644
Size: 269 234 61 618 143 354 3156
Fragments arranged by size:
3156 618 354 269 234 143 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 279 297 446 449 2418 2551 3010 3219 279
Size: 18 149 3 1969 133 459 209 1895
Fragments arranged by size:
1969 1895 459 209 149 133 18 3
TatI w'GTAC_w
Cuts at: 1595 4350 1595
Size: 2755 2080
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 286 318 1028 1060 4352 4384 286
Size: 32 710 32 3292 32 737
Fragments arranged by size:
3292 737 710 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2632 2632
Size: 4835
XbaI T'CTAG_A
Cuts at: 1866 1866
Size: 4835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 911 961 1815 2330 911
Size: 50 854 515 3416
Fragments arranged by size:
3416 854 515 50
XhoI C'TCGA_G
Cuts at: 980 980
Size: 4835
XmaI C'CCGG_G
Cuts at: 1855 1855
Size: 4835
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI ApaI AscI AvrII
BaeI BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI EcoNI FseI FspAI HpaI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XmnI ZraI