Restriction map for
pF1KB9984
(Circular) (Six-base) MAPSORT of: pF1KB9984.seq Check: 8100 from: 1 to: 5246
pF1KB9984 5246 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 410 2284 410
Size: 1874 3372
Acc65I G'GTAC_C
Cuts at: 2262 2262
Size: 5246
AclI AA'CG_TT
Cuts at: 5086 5086
Size: 5246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 720 3071 3503 4250 4518 523
Size: 197 2351 432 747 268 1251
Fragments arranged by size:
2351 1251 747 432 268 197
AflIII A'CryG_T
Cuts at: 2598 3702 4679 2598
Size: 1104 977 3165
Fragments arranged by size:
3165 1104 977
AgeI A'CCGG_T
Cuts at: 3634 3634
Size: 5246
AhdI GACnn_n'nnGTC
Cuts at: 563 563
Size: 5246
AleI CACnn'nnGTG
Cuts at: 1552 1552
Size: 5246
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1399 1431 1399
Size: 32 5214
AlwNI CAG_nnn'CTG
Cuts at: 1230 2004 2551 4118 1230
Size: 774 547 1567 2358
Fragments arranged by size:
2358 1567 774 547
ApaI G_GGCC'C
Cuts at: 147 147
Size: 5246
ApaLI G'TGCA_C
Cuts at: 192 4016 192
Size: 3824 1422
ApoI r'AATT_y
Cuts at: 1021 1618 2023 2227 2250 4599 4855 1021
Size: 597 405 204 23 2349 256 1412
Fragments arranged by size:
2349 1412 597 405 256 204 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 5246
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5246
AvaI C'yCGr_G
Cuts at: 139 208 911 1101 1662 2266 139
Size: 69 703 190 561 604 3119
Fragments arranged by size:
3119 703 604 561 190 69
BamHI G'GATC_C
Cuts at: 2271 2271
Size: 5246
BanI G'GyrC_C
Cuts at: 748 1095 1389 1766 2262 2676 2924 2959 748
Size: 347 294 377 496 414 248 35 3035
Fragments arranged by size:
3035 496 414 377 347 294 248 35
BanII G_rGCy'C
Cuts at: 147 599 1612 2260 3290 147
Size: 452 1013 648 1030 2103
Fragments arranged by size:
2103 1030 1013 648 452
BbeI G_GCGC'C
Cuts at: 2680 2928 2680
Size: 248 4998
BbsI GAAGACnn'nnnn_
Cuts at: 111 111
Size: 5246
BciVI GTATCCnnnnn_n'
Cuts at: 210 313 3144 3905 5246 210
Size: 103 2831 761 1341 210
Fragments arranged by size:
2831 1341 761 210 103
BclI T'GATC_A
Cuts at: 2766 2766
Size: 5246
BglI GCCn_nnn'nGGC
Cuts at: 518 934 1898 518
Size: 416 964 3866
Fragments arranged by size:
3866 964 416
BglII A'GATC_T
Cuts at: 1056 1666 2761 4798 1056
Size: 610 1095 2037 1504
Fragments arranged by size:
2037 1504 1095 610
BlpI GC'TnA_GC
Cuts at: 2358 2358
Size: 5246
Bme1580I G_kGCm'C
Cuts at: 147 196 1100 1650 1724 2871 2964 4020 147
Size: 49 904 550 74 1147 93 1056 1373
Fragments arranged by size:
1373 1147 1056 904 550 93 74 49
BmgBI CAC'GTC
Cuts at: 783 783
Size: 5246
BmrI ACTGGGnnnn_n'
Cuts at: 1763 2458 2539 2874 5018 1763
Size: 695 81 335 2144 1991
Fragments arranged by size:
2144 1991 695 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3613 3613
Size: 5246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 1805 797
Size: 1008 4238
Bpu10I CC'TnA_GC
Cuts at: 593 1856 593
Size: 1263 3983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1179 1278 2422 3301 3793 4091 1179
Size: 99 1144 879 492 298 2334
Fragments arranged by size:
2334 1144 879 492 298 99
BsaI GGTCTCn'nnnn_
Cuts at: 4950 4950
Size: 5246
BsaAI yAC'GTr
Cuts at: 2599 2599
Size: 5246
BsaBI GATnn'nnATC
Cuts at: 6 2760 4797 6
Size: 2754 2037 455
Fragments arranged by size:
2754 2037 455
BsaHI Gr'CG_yC
Cuts at: 2677 2925 3627 5123 2677
Size: 248 702 1496 2800
Fragments arranged by size:
2800 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1763 2435 2956 3634 3908 4055 4466 1763
Size: 672 521 678 274 147 411 2543
Fragments arranged by size:
2543 678 672 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 165 195 1114 1144 1346 1376 165
Size: 30 919 30 202 30 4035
Fragments arranged by size:
4035 919 202 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 708 1186 1272 708
Size: 478 86 4682
Fragments arranged by size:
4682 478 86
BseYI C'CCAG_C
Cuts at: 2671 3429 4006 4502 2671
Size: 758 577 496 3415
Fragments arranged by size:
3415 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 668
Size: 5246
BsiEI CG_ry'CG
Cuts at: 104 2788 2834 4042 104
Size: 2684 46 1208 1308
Fragments arranged by size:
2684 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 196 805 1683 2200 2260 3038 3228 4020 196
Size: 609 878 517 60 778 190 792 1422
Fragments arranged by size:
1422 878 792 778 609 517 190 60
BsmI GAATG_Cn'
Cuts at: 1785 1785
Size: 5246
BsmBI CGTCTCn'nnnn_
Cuts at: 761 773 1781 761
Size: 12 1008 4226
Fragments arranged by size:
4226 1008 12
Bsp1286I G_dGCh'C
Cuts at: 147 196 599 805 1100 1612 1650 1683 1724
Size: 49 403 206 295 512 38 33 41
Cuts at: 1724 2200 2260 2871 2964 3038 3228 3290 4020
Size: 476 60 611 93 74 190 62 730
Cuts at: 4020 147
Size: 1373
Fragments arranged by size:
1373 730 611 512 476 403 295 206
190 93 74 62 60 49 41 38
33
BspEI T'CCGG_A
Cuts at: 4466 4466
Size: 5246
BspHI T'CATG_A
Cuts at: 4422 4422
Size: 5246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1285 2296 2510 2812 3193 1285
Size: 1011 214 302 381 3338
Fragments arranged by size:
3338 1011 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3538 3592 5076 5241 50
Size: 3488 54 1484 165 55
Fragments arranged by size:
3488 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 311 1307 1737 3158 311
Size: 996 430 1421 2399
Fragments arranged by size:
2399 1421 996 430
BsrFI r'CCGG_y
Cuts at: 3244 3425 3634 3244
Size: 181 209 4856
Fragments arranged by size:
4856 209 181
BsrGI T'GTAC_A
Cuts at: 1364 1364
Size: 5246
BssSI C'ACGA_G
Cuts at: 3517 3875 3517
Size: 358 4888
BstAPI GCAn_nnn'nTGC
Cuts at: 1230 1879 2623 1230
Size: 649 744 3853
Fragments arranged by size:
3853 744 649
BstBI TT'CG_AA
Cuts at: 3607 3607
Size: 5246
BstXI CCAn_nnnn'nTGG
Cuts at: 1033 1252 1347 1033
Size: 219 95 4932
Fragments arranged by size:
4932 219 95
BstYI r'GATC_y
Cuts at: 1 1056 1666 2038 2271 2738 2761 3096 3342
Size: 1055 610 372 233 467 23 335 246
Cuts at: 3342 4354 4440 4452 4798 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1055 1012 610 467 449 372 346 335
246 233 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2233 2233
Size: 5246
BtgI C'CryG_G
Cuts at: 449 1553 1583 1943 1961 2127 449
Size: 1104 30 360 18 166 3568
Fragments arranged by size:
3568 1104 360 166 30 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3196 3377 3540 3196
Size: 181 163 4902
Fragments arranged by size:
4902 181 163
BtsI GCAGTG_nn'
Cuts at: 1142 1275 1367 1434 2489 1142
Size: 133 92 67 1055 3899
Fragments arranged by size:
3899 1055 133 92 67
DraI TTT'AAA
Cuts at: 2245 2245
Size: 5246
DraIII CAC_nnn'GTG
Cuts at: 943 2111 943
Size: 1168 4078
DrdI GACnn_nn'nnGTC
Cuts at: 2952 3810 2952
Size: 858 4388
EaeI y'GGCC_r
Cuts at: 165 614 2117 2831 3005 3396 3423 4768 165
Size: 449 1503 714 174 391 27 1345 643
Fragments arranged by size:
1503 1345 714 643 449 391 174 27
EagI C'GGCC_G
Cuts at: 2831 2831
Size: 5246
EarI CTCTTCn'nnn_
Cuts at: 243 1600 3269 3479 243
Size: 1357 1669 210 2010
Fragments arranged by size:
2010 1669 1357 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 418 970 3454 3764 3910 5056 418
Size: 552 2484 310 146 1146 608
Fragments arranged by size:
2484 1146 608 552 310 146
EcoICRI GAG'CTC
Cuts at: 2258 2258
Size: 5246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 720 797 1805 3071 3503 4250 4518 523
Size: 197 77 1008 1266 432 747 268 1251
Fragments arranged by size:
1266 1251 1008 747 432 268 197 77
EcoNI CCTnn'n_nnAGG
Cuts at: 1463 1996 2236 1463
Size: 533 240 4473
Fragments arranged by size:
4473 533 240
EcoO109I rG'GnC_Cy
Cuts at: 143 144 816 915 2385 143
Size: 1 672 99 1470 3004
Fragments arranged by size:
3004 1470 672 99 1
EcoRI G'AATT_C
Cuts at: 2227 2250 2227
Size: 23 5223
EcoRV GAT'ATC
Cuts at: 1471 1471
Size: 5246
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1489 2079 3265 1489
Size: 590 1186 3470
Fragments arranged by size:
3470 1186 590
FspI TGC'GCA
Cuts at: 1234 3027 1234
Size: 1793 3453
HaeII r_GCGC'y
Cuts at: 2680 2928 3950 4929 2680
Size: 248 1022 979 2997
Fragments arranged by size:
2997 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 530 549 562 581 805 837 1651 1656 1658
Size: 19 13 19 224 32 814 5 2
Cuts at: 1658 1683 1688 1690 3088 3118 3120 3150 530
Size: 25 5 2 1398 30 2 30 2626
Fragments arranged by size:
2626 1398 814 224 32 30 30 25
19 19 13 5 5 2 2 2
HincII GTy'rAC
Cuts at: 2285 2285
Size: 5246
KasI G'GCGC_C
Cuts at: 2676 2924 2676
Size: 248 4998
KpnI G_GTAC'C
Cuts at: 2266 2266
Size: 5246
MluI A'CGCG_T
Cuts at: 4679 4679
Size: 5246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3917 4101 3917
Size: 184 5062
MscI TGG'CCA
Cuts at: 616 2119 3007 616
Size: 1503 888 2855
Fragments arranged by size:
2855 1503 888
MslI CAynn'nnrTG
Cuts at: 443 1552 3235 3362 443
Size: 1109 1683 127 2327
Fragments arranged by size:
2327 1683 1109 127
MspA1I CmG'CkG
Cuts at: 272 483 535 610 635 2357 2671 3031 4044
Size: 211 52 75 25 1722 314 360 1013
Cuts at: 4044 4289 272
Size: 245 1229
Fragments arranged by size:
1722 1229 1013 360 314 245 211 75
52 25
NaeI GCC'GGC
Cuts at: 3427 3427
Size: 5246
NarI GG'CG_CC
Cuts at: 2677 2925 2677
Size: 248 4998
NcoI C'CATG_G
Cuts at: 1583 1943 2127 1583
Size: 360 184 4702
Fragments arranged by size:
4702 360 184
NdeI CA'TA_TG
Cuts at: 2142 2142
Size: 5246
NgoMIV G'CCGG_C
Cuts at: 3425 3425
Size: 5246
NspI r_CATG'y
Cuts at: 2299 2799 3706 2299
Size: 500 907 3839
Fragments arranged by size:
3839 907 500
PasI CC'CwG_GG
Cuts at: 1651 1651
Size: 5246
PciI A'CATG_T
Cuts at: 3702 3702
Size: 5246
PfoI T'CCnGG_A
Cuts at: 2213 2213
Size: 5246
PmeI GTTT'AAAC
Cuts at: 2245 2245
Size: 5246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4385 4417 4385
Size: 32 5214
PpuMI rG'GwC_Cy
Cuts at: 816 915 816
Size: 99 5147
PspOMI G'GGCC_C
Cuts at: 143 143
Size: 5246
PstI C_TGCA'G
Cuts at: 1374 1569 2293 2978 1374
Size: 195 724 685 3642
Fragments arranged by size:
3642 724 685 195
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5246
PvuII CAG'CTG
Cuts at: 272 483 610 635 2671 3031 272
Size: 211 127 25 2036 360 2487
Fragments arranged by size:
2487 2036 360 211 127 25
RsrII CG'GwC_CG
Cuts at: 3441 3441
Size: 5246
SacI G_AGCT'C
Cuts at: 2260 2260
Size: 5246
SalI G'TCGA_C
Cuts at: 2283 2283
Size: 5246
SanDI GG'GwC_CC
Cuts at: 915 915
Size: 5246
SapI GCTCTTCn'nnn_
Cuts at: 1600 3269 3479 1600
Size: 1669 210 3367
Fragments arranged by size:
3367 1669 210
SbfI CC_TGCA'GG
Cuts at: 2293 2293
Size: 5246
ScaI AGT'ACT
Cuts at: 4763 4763
Size: 5246
SfcI C'TryA_G
Cuts at: 33 395 551 1370 1565 2289 2974 3967 4158
Size: 362 156 819 195 724 685 993 191
Cuts at: 4158 33
Size: 1121
Fragments arranged by size:
1121 993 819 724 685 362 195 191
156
SfoI GGC'GCC
Cuts at: 2678 2926 2678
Size: 248 4998
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5246
SmaI CCC'GGG
Cuts at: 913 2268 913
Size: 1355 3891
SmlI C'TyrA_G
Cuts at: 1158 1257 2401 3316 3808 4070 1158
Size: 99 1144 915 492 262 2334
Fragments arranged by size:
2334 1144 915 492 262 99
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5246
SphI G_CATG'C
Cuts at: 2299 2799 2299
Size: 500 4746
SspI AAT'ATT
Cuts at: 1630 2152 1630
Size: 522 4724
StuI AGG'CCT
Cuts at: 371 521 371
Size: 150 5096
StyI C'CwwG_G
Cuts at: 1125 1583 1943 2127 2380 2734 1125
Size: 458 360 184 253 354 3637
Fragments arranged by size:
3637 458 360 354 253 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 433 2829 2962 3421 3630 433
Size: 2396 133 459 209 2049
Fragments arranged by size:
2396 2049 459 209 133
TatI w'GTAC_w
Cuts at: 1364 4761 1364
Size: 3397 1849
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 165 197 1007 1039 1346 1378 4763 4795 165
Size: 32 810 32 307 32 3385 32 616
Fragments arranged by size:
3385 810 616 307 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 155 3043 155
Size: 2888 2358
XbaI T'CTAG_A
Cuts at: 2277 2277
Size: 5246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 575 2741 575
Size: 2166 3080
XmaI C'CCGG_G
Cuts at: 911 2266 911
Size: 1355 3891
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsiWI BssHII BstEII BstZ17I
ClaI CspCI FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI