Restriction map for
pF1KB9981
(Circular) (Six-base) MAPSORT of: pF1KB9981.seq Check: 9667 from: 1 to: 4796
pF1KB9981 4796 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 705 705
Size: 4796
AccI GT'mk_AC
Cuts at: 124 548 1834 124
Size: 424 1286 3086
Fragments arranged by size:
3086 1286 424
Acc65I G'GTAC_C
Cuts at: 1812 1812
Size: 4796
AclI AA'CG_TT
Cuts at: 4636 4636
Size: 4796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 2621 3053 3800 4068 1285
Size: 1336 432 747 268 2013
Fragments arranged by size:
2013 1336 747 432 268
AflIII A'CryG_T
Cuts at: 2148 3252 4229 2148
Size: 1104 977 2715
Fragments arranged by size:
2715 1104 977
AgeI A'CCGG_T
Cuts at: 3184 3184
Size: 4796
AlwNI CAG_nnn'CTG
Cuts at: 177 957 1413 1597 1755 2101 3668 177
Size: 780 456 184 158 346 1567 1305
Fragments arranged by size:
1567 1305 780 456 346 184 158
ApaI G_GGCC'C
Cuts at: 749 749
Size: 4796
ApaLI G'TGCA_C
Cuts at: 3566 3566
Size: 4796
ApoI r'AATT_y
Cuts at: 1800 4149 4405 1800
Size: 2349 256 2191
Fragments arranged by size:
2349 2191 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4796
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
AvaI C'yCGr_G
Cuts at: 137 629 814 1333 1816 137
Size: 492 185 519 483 3117
Fragments arranged by size:
3117 519 492 483 185
BamHI G'GATC_C
Cuts at: 1170 1320 1821 1170
Size: 150 501 4145
Fragments arranged by size:
4145 501 150
BanI G'GyrC_C
Cuts at: 245 414 576 1350 1812 2226 2474 2509 245
Size: 169 162 774 462 414 248 35 2532
Fragments arranged by size:
2532 774 462 414 248 169 162 35
BanII G_rGCy'C
Cuts at: 749 911 1810 2840 749
Size: 162 899 1030 2705
Fragments arranged by size:
2705 1030 899 162
BbeI G_GCGC'C
Cuts at: 1354 2230 2478 1354
Size: 876 248 3672
Fragments arranged by size:
3672 876 248
BbvCI CC'TCA_GC
Cuts at: 999 999
Size: 4796
BciVI GTATCCnnnnn_n'
Cuts at: 2694 3455 4796 2694
Size: 761 1341 2694
Fragments arranged by size:
2694 1341 761
BclI T'GATC_A
Cuts at: 2316 2316
Size: 4796
BglI GCCn_nnn'nGGC
Cuts at: 272 1094 1459 272
Size: 822 365 3609
Fragments arranged by size:
3609 822 365
BglII A'GATC_T
Cuts at: 714 1401 2311 4348 714
Size: 687 910 2037 1162
Fragments arranged by size:
2037 1162 910 687
BlpI GC'TnA_GC
Cuts at: 1908 1908
Size: 4796
Bme1580I G_kGCm'C
Cuts at: 144 284 560 581 749 850 1073 1523 2421
Size: 140 276 21 168 101 223 450 898
Cuts at: 2421 2514 3570 144
Size: 93 1056 1370
Fragments arranged by size:
1370 1056 898 450 276 223 168 140
101 93 21
BmgBI CAC'GTC
Cuts at: 122 122
Size: 4796
BmrI ACTGGGnnnn_n'
Cuts at: 1060 1648 2008 2089 2424 4568 1060
Size: 588 360 81 335 2144 1288
Fragments arranged by size:
2144 1288 588 360 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 4796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 567 671 739 887 935 1772 437
Size: 130 104 68 148 48 837 3461
Fragments arranged by size:
3461 837 148 130 104 68 48
Bpu10I CC'TnA_GC
Cuts at: 999 1283 999
Size: 284 4512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1972 2851 3343 3641 1972
Size: 879 492 298 3127
Fragments arranged by size:
3127 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4500 4500
Size: 4796
BsaAI yAC'GTr
Cuts at: 2149 2149
Size: 4796
BsaBI GATnn'nnATC
Cuts at: 6 2310 4347 6
Size: 2304 2037 455
Fragments arranged by size:
2304 2037 455
BsaHI Gr'CG_yC
Cuts at: 1351 2227 2475 3177 4673 1351
Size: 876 248 702 1496 1474
Fragments arranged by size:
1496 1474 876 702 248
BsaWI w'CCGG_w
Cuts at: 1396 1985 2506 3184 3458 3605 4016 1396
Size: 589 521 678 274 147 411 2176
Fragments arranged by size:
2176 678 589 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1351 1381 1351
Size: 30 4766
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1258 1258
Size: 4796
BseYI C'CCAG_C
Cuts at: 128 315 328 348 353 423 515 879 950
Size: 187 13 20 5 70 92 364 71
Cuts at: 950 1054 1200 1426 2221 2979 3556 4052 128
Size: 104 146 226 795 758 577 496 872
Fragments arranged by size:
872 795 758 577 496 364 226 187
146 104 92 71 70 20 13 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1296 1744 1296
Size: 448 4348
BsiEI CG_ry'CG
Cuts at: 104 2338 2384 3592 104
Size: 2234 46 1208 1308
Fragments arranged by size:
2234 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1614 1810 2588 2778 3570 1614
Size: 196 778 190 792 2840
Fragments arranged by size:
2840 792 778 196 190
BsmI GAATG_Cn'
Cuts at: 1229 1229
Size: 4796
Bsp1286I G_dGCh'C
Cuts at: 144 284 560 581 749 850 911 1073 1523
Size: 140 276 21 168 101 61 162 450
Cuts at: 1523 1614 1810 2421 2514 2588 2778 2840 3570
Size: 91 196 611 93 74 190 62 730
Cuts at: 3570 144
Size: 1370
Fragments arranged by size:
1370 730 611 450 276 196 190 168
162 140 101 93 91 74 62 61
21
BspEI T'CCGG_A
Cuts at: 4016 4016
Size: 4796
BspHI T'CATG_A
Cuts at: 3972 3972
Size: 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 705 1618 1846 2060 2362 2743 705
Size: 913 228 214 302 381 2758
Fragments arranged by size:
2758 913 381 302 228 214
BsrBI CCG'CTC
Cuts at: 50 3088 3142 4626 4791 50
Size: 3038 54 1484 165 55
Fragments arranged by size:
3038 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 113 396 1438 1648 2708 113
Size: 283 1042 210 1060 2201
Fragments arranged by size:
2201 1060 1042 283 210
BsrFI r'CCGG_y
Cuts at: 1109 1347 1583 2794 2975 3184 1109
Size: 238 236 1211 181 209 2721
Fragments arranged by size:
2721 1211 238 236 209 181
BssSI C'ACGA_G
Cuts at: 3067 3425 3067
Size: 358 4438
BstAPI GCAn_nnn'nTGC
Cuts at: 1597 1755 2173 1597
Size: 158 418 4220
Fragments arranged by size:
4220 418 158
BstBI TT'CG_AA
Cuts at: 3157 3157
Size: 4796
BstXI CCAn_nnnn'nTGG
Cuts at: 1665 1665
Size: 4796
BstYI r'GATC_y
Cuts at: 1 714 860 1170 1288 1320 1401 1821 2288
Size: 713 146 310 118 32 81 420 467
Cuts at: 2288 2311 2646 2892 3904 3990 4002 4348 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 713 467 449 420 346 335 310
246 146 118 86 81 32 23 12
Bsu36I CC'TnA_GG
Cuts at: 444 1046 444
Size: 602 4194
BtgI C'CryG_G
Cuts at: 107 1466 107
Size: 1359 3437
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1537 2746 2927 3090 1537
Size: 1209 181 163 3243
Fragments arranged by size:
3243 1209 181 163
BtsI GCAGTG_nn'
Cuts at: 1732 2039 1732
Size: 307 4489
DraI TTT'AAA
Cuts at: 1795 1795
Size: 4796
DrdI GACnn_nn'nnGTC
Cuts at: 2502 3360 2502
Size: 858 3938
EaeI y'GGCC_r
Cuts at: 110 264 959 1238 2381 2555 2946 2973 4318
Size: 154 695 279 1143 174 391 27 1345
Cuts at: 4318 110
Size: 588
Fragments arranged by size:
1345 1143 695 588 391 279 174 154
27
EagI C'GGCC_G
Cuts at: 2381 2381
Size: 4796
EarI CTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 175 3004 3314 3460 4606 175
Size: 2829 310 146 1146 365
Fragments arranged by size:
2829 1146 365 310 146
EcoICRI GAG'CTC
Cuts at: 1808 1808
Size: 4796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 567 671 739 887 935 1285 1772 2621
Size: 130 104 68 148 48 350 487 849
Cuts at: 2621 3053 3800 4068 437
Size: 432 747 268 1165
Fragments arranged by size:
1165 849 747 487 432 350 268 148
130 104 68 48
EcoNI CCTnn'n_nnAGG
Cuts at: 1082 1082
Size: 4796
EcoO109I rG'GnC_Cy
Cuts at: 207 448 512 745 746 986 1050 1153 1295
Size: 241 64 233 1 240 64 103 142
Cuts at: 1295 1362 1752 1935 207
Size: 67 390 183 3068
Fragments arranged by size:
3068 390 241 240 233 183 142 103
67 64 64 1
EcoRI G'AATT_C
Cuts at: 1800 1800
Size: 4796
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4796
FspI TGC'GCA
Cuts at: 2577 2577
Size: 4796
HaeII r_GCGC'y
Cuts at: 227 1354 2230 2478 3500 4479 227
Size: 1127 876 248 1022 979 544
Fragments arranged by size:
1127 1022 979 876 544 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 182 214 997 1029 1351 1383 1601 1633 2638
Size: 32 783 32 322 32 218 32 1005
Cuts at: 2638 2668 2670 2700 182
Size: 30 2 30 2278
Fragments arranged by size:
2278 1005 783 322 218 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 125 1835 125
Size: 1710 3086
KasI G'GCGC_C
Cuts at: 1350 2226 2474 1350
Size: 876 248 3672
Fragments arranged by size:
3672 876 248
KpnI G_GTAC'C
Cuts at: 1816 1816
Size: 4796
MluI A'CGCG_T
Cuts at: 4229 4229
Size: 4796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 463 794 3467 3651 463
Size: 331 2673 184 1608
Fragments arranged by size:
2673 1608 331 184
MscI TGG'CCA
Cuts at: 112 961 2557 112
Size: 849 1596 2351
Fragments arranged by size:
2351 1596 849
MslI CAynn'nnrTG
Cuts at: 389 1214 1234 2785 2912 389
Size: 825 20 1551 127 2273
Fragments arranged by size:
2273 1551 825 127 20
MspA1I CmG'CkG
Cuts at: 132 160 457 519 1003 1907 2221 2581 3594
Size: 28 297 62 484 904 314 360 1013
Cuts at: 3594 3839 132
Size: 245 1089
Fragments arranged by size:
1089 1013 904 484 360 314 297 245
62 28
NaeI GCC'GGC
Cuts at: 1349 1585 2977 1349
Size: 236 1392 3168
Fragments arranged by size:
3168 1392 236
NarI GG'CG_CC
Cuts at: 1351 2227 2475 1351
Size: 876 248 3672
Fragments arranged by size:
3672 876 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4796
NgoMIV G'CCGG_C
Cuts at: 1347 1583 2975 1347
Size: 236 1392 3168
Fragments arranged by size:
3168 1392 236
NspI r_CATG'y
Cuts at: 1849 2349 3256 1849
Size: 500 907 3389
Fragments arranged by size:
3389 907 500
PasI CC'CwG_GG
Cuts at: 1372 1578 1686 1372
Size: 206 108 4482
Fragments arranged by size:
4482 206 108
PciI A'CATG_T
Cuts at: 3252 3252
Size: 4796
PflMI CCAn_nnn'nTGG
Cuts at: 957 1719 957
Size: 762 4034
PfoI T'CCnGG_A
Cuts at: 1103 1103
Size: 4796
PmeI GTTT'AAAC
Cuts at: 1795 1795
Size: 4796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 4764
PpuMI rG'GwC_Cy
Cuts at: 207 448 512 207
Size: 241 64 4491
Fragments arranged by size:
4491 241 64
PspOMI G'GGCC_C
Cuts at: 745 745
Size: 4796
PstI C_TGCA'G
Cuts at: 215 702 1409 1460 1629 1843 2528 215
Size: 487 707 51 169 214 685 2483
Fragments arranged by size:
2483 707 685 487 214 169 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4796
PvuII CAG'CTG
Cuts at: 132 457 519 1003 2221 2581 132
Size: 325 62 484 1218 360 2347
Fragments arranged by size:
2347 1218 484 360 325 62
RsrII CG'GwC_CG
Cuts at: 2991 2991
Size: 4796
SacI G_AGCT'C
Cuts at: 1810 1810
Size: 4796
SalI G'TCGA_C
Cuts at: 123 1833 123
Size: 1710 3086
SanDI GG'GwC_CC
Cuts at: 512 512
Size: 4796
SapI GCTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
SbfI CC_TGCA'GG
Cuts at: 1460 1843 1460
Size: 383 4413
ScaI AGT'ACT
Cuts at: 4313 4313
Size: 4796
SfcI C'TryA_G
Cuts at: 33 211 698 1405 1456 1625 1839 2524 3517
Size: 178 487 707 51 169 214 685 993
Cuts at: 3517 3708 33
Size: 191 1121
Fragments arranged by size:
1121 993 707 685 487 214 191 178
169 51
SfoI GGC'GCC
Cuts at: 1352 2228 2476 1352
Size: 876 248 3672
Fragments arranged by size:
3672 876 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
SmaI CCC'GGG
Cuts at: 139 631 1818 139
Size: 492 1187 3117
Fragments arranged by size:
3117 1187 492
SmlI C'TyrA_G
Cuts at: 1951 2866 3358 3620 1951
Size: 915 492 262 3127
Fragments arranged by size:
3127 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4796
SphI G_CATG'C
Cuts at: 1849 2349 1849
Size: 500 4296
SrfI GCCC'GGGC
Cuts at: 139 139
Size: 4796
StuI AGG'CCT
Cuts at: 1309 1309
Size: 4796
StyI C'CwwG_G
Cuts at: 107 539 920 1157 1930 2284 107
Size: 432 381 237 773 354 2619
Fragments arranged by size:
2619 773 432 381 354 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 336 2379 2512 2971 3180 336
Size: 2043 133 459 209 1952
Fragments arranged by size:
2043 1952 459 209 133
TatI w'GTAC_w
Cuts at: 1415 4311 1415
Size: 2896 1900
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 229 261 1305 1337 4313 4345 229
Size: 32 1044 32 2976 32 680
Fragments arranged by size:
2976 1044 680 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1255 2593 1255
Size: 1338 3458
XbaI T'CTAG_A
Cuts at: 1827 1827
Size: 4796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 537 719 1716 2291 537
Size: 182 997 575 3042
Fragments arranged by size:
3042 997 575 182
XmaI C'CCGG_G
Cuts at: 137 629 1816 137
Size: 492 1187 3117
Fragments arranged by size:
3117 1187 492
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SspI SwaI XhoI
XmnI ZraI