Restriction map for
pF1KB9943
(Circular) (Six-base) MAPSORT of: pF1KB9943.seq Check: 3710 from: 1 to: 4604
pF1KB9943 4604 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 770 1642 770
Size: 872 3732
Acc65I G'GTAC_C
Cuts at: 1620 1620
Size: 4604
AclI AA'CG_TT
Cuts at: 4444 4444
Size: 4604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 256 387 505 811 2429 2861 3608 3876
Size: 26 131 118 306 1618 432 747 268
Cuts at: 3876 230
Size: 958
Fragments arranged by size:
1618 958 747 432 306 268 131 118
26
AflIII A'CryG_T
Cuts at: 1956 3060 4037 1956
Size: 1104 977 2523
Fragments arranged by size:
2523 1104 977
AgeI A'CCGG_T
Cuts at: 2992 2992
Size: 4604
AleI CACnn'nnGTG
Cuts at: 1105 1105
Size: 4604
AlwNI CAG_nnn'CTG
Cuts at: 1531 1909 3476 1531
Size: 378 1567 2659
Fragments arranged by size:
2659 1567 378
ApaLI G'TGCA_C
Cuts at: 3374 3374
Size: 4604
ApoI r'AATT_y
Cuts at: 254 313 334 820 1070 1345 1418 1608 3957
Size: 59 21 486 250 275 73 190 2349
Cuts at: 3957 4213 254
Size: 256 645
Fragments arranged by size:
2349 645 486 275 256 250 190 73
59 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 4604
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
AvaI C'yCGr_G
Cuts at: 1624 1624
Size: 4604
BamHI G'GATC_C
Cuts at: 1629 1629
Size: 4604
BanI G'GyrC_C
Cuts at: 850 1620 2034 2282 2317 850
Size: 770 414 248 35 3137
Fragments arranged by size:
3137 770 414 248 35
BanII G_rGCy'C
Cuts at: 1618 2648 1618
Size: 1030 3574
BbeI G_GCGC'C
Cuts at: 2038 2286 2038
Size: 248 4356
BbsI GAAGACnn'nnnn_
Cuts at: 667 667
Size: 4604
BciVI GTATCCnnnnn_n'
Cuts at: 2502 3263 4604 2502
Size: 761 1341 2502
Fragments arranged by size:
2502 1341 761
BclI T'GATC_A
Cuts at: 867 2124 867
Size: 1257 3347
BglII A'GATC_T
Cuts at: 2119 4156 2119
Size: 2037 2567
BlpI GC'TnA_GC
Cuts at: 1716 1716
Size: 4604
Bme1580I G_kGCm'C
Cuts at: 2229 2322 3378 2229
Size: 93 1056 3455
Fragments arranged by size:
3455 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1227 1816 1897 2232 4376 1227
Size: 589 81 335 2144 1455
Fragments arranged by size:
2144 1455 589 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1593 1593
Size: 4604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 738 1780 2659 3151 3449 738
Size: 1042 879 492 298 1893
Fragments arranged by size:
1893 1042 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4308 4308
Size: 4604
BsaAI yAC'GTr
Cuts at: 1957 1957
Size: 4604
BsaBI GATnn'nnATC
Cuts at: 6 866 2118 4155 6
Size: 860 1252 2037 455
Fragments arranged by size:
2037 1252 860 455
BsaHI Gr'CG_yC
Cuts at: 208 2035 2283 2985 4481 208
Size: 1827 248 702 1496 331
Fragments arranged by size:
1827 1496 702 331 248
BsaWI w'CCGG_w
Cuts at: 1793 2314 2992 3266 3413 3824 1793
Size: 521 678 274 147 411 2573
Fragments arranged by size:
2573 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1322 2029 2787 3364 3860 1322
Size: 707 758 577 496 2066
Fragments arranged by size:
2066 758 707 577 496
BsiEI CG_ry'CG
Cuts at: 104 2146 2192 3400 104
Size: 2042 46 1208 1308
Fragments arranged by size:
2042 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1618 2396 2586 3378 1618
Size: 778 190 792 2844
Fragments arranged by size:
2844 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1618 2229 2322 2396 2586 2648 3378 1618
Size: 611 93 74 190 62 730 2844
Fragments arranged by size:
2844 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3824 3824
Size: 4604
BspHI T'CATG_A
Cuts at: 3780 3780
Size: 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1654 1868 2170 2551 1654
Size: 214 302 381 3707
Fragments arranged by size:
3707 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2896 2950 4434 4599 50
Size: 2846 54 1484 165 55
Fragments arranged by size:
2846 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2516 2516
Size: 4604
BsrFI r'CCGG_y
Cuts at: 193 2602 2783 2992 193
Size: 2409 181 209 1805
Fragments arranged by size:
2409 1805 209 181
BssSI C'ACGA_G
Cuts at: 2875 3233 2875
Size: 358 4246
BstAPI GCAn_nnn'nTGC
Cuts at: 420 1981 420
Size: 1561 3043
BstBI TT'CG_AA
Cuts at: 2965 2965
Size: 4604
BstYI r'GATC_y
Cuts at: 1 1461 1629 2096 2119 2454 2700 3712 3798
Size: 1460 168 467 23 335 246 1012 86
Cuts at: 3798 3810 4156 1
Size: 12 346 449
Fragments arranged by size:
1460 1012 467 449 346 335 246 168
86 23 12
BtgI C'CryG_G
Cuts at: 107 149 107
Size: 42 4562
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2554 2735 2898 2554
Size: 181 163 4260
Fragments arranged by size:
4260 181 163
BtsI GCAGTG_nn'
Cuts at: 1847 1847
Size: 4604
DraI TTT'AAA
Cuts at: 339 615 1603 339
Size: 276 988 3340
Fragments arranged by size:
3340 988 276
DrdI GACnn_nn'nnGTC
Cuts at: 2310 3168 2310
Size: 858 3746
EaeI y'GGCC_r
Cuts at: 110 143 182 491 2189 2363 2754 2781 4126
Size: 33 39 309 1698 174 391 27 1345
Cuts at: 4126 110
Size: 588
Fragments arranged by size:
1698 1345 588 391 309 174 39 33
27
EagI C'GGCC_G
Cuts at: 2189 2189
Size: 4604
EarI CTCTTCn'nnn_
Cuts at: 614 635 650 794 797 1244 1587 2627 2837
Size: 21 15 144 3 447 343 1040 210
Cuts at: 2837 614
Size: 2381
Fragments arranged by size:
2381 1040 447 343 210 144 21 15
3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2812 3122 3268 4414 2812
Size: 310 146 1146 3002
Fragments arranged by size:
3002 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1616 1616
Size: 4604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 256 387 505 811 1593 2429 2861 3608
Size: 26 131 118 306 782 836 432 747
Cuts at: 3608 3876 230
Size: 268 958
Fragments arranged by size:
958 836 782 747 432 306 268 131
118 26
EcoO109I rG'GnC_Cy
Cuts at: 1743 1743
Size: 4604
EcoRI G'AATT_C
Cuts at: 254 1608 254
Size: 1354 3250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4604
FspI TGC'GCA
Cuts at: 2385 2385
Size: 4604
HaeII r_GCGC'y
Cuts at: 217 1287 2038 2286 3308 4287 217
Size: 1070 751 248 1022 979 534
Fragments arranged by size:
1070 1022 979 751 534 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 461 493 2446 2476 2478 2508 461
Size: 32 1953 30 2 30 2557
Fragments arranged by size:
2557 1953 32 30 30 2
HincII GTy'rAC
Cuts at: 1643 1643
Size: 4604
HindIII A'AGCT_T
Cuts at: 737 1184 737
Size: 447 4157
KasI G'GCGC_C
Cuts at: 2034 2282 2034
Size: 248 4356
KpnI G_GTAC'C
Cuts at: 1624 1624
Size: 4604
MfeI C'AATT_G
Cuts at: 487 487
Size: 4604
MluI A'CGCG_T
Cuts at: 4037 4037
Size: 4604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1591 3275 3459 1591
Size: 1684 184 2736
Fragments arranged by size:
2736 1684 184
MscI TGG'CCA
Cuts at: 493 2365 493
Size: 1872 2732
MslI CAynn'nnrTG
Cuts at: 506 1105 2593 2720 506
Size: 599 1488 127 2390
Fragments arranged by size:
2390 1488 599 127
MspA1I CmG'CkG
Cuts at: 151 1715 2029 2389 3402 3647 151
Size: 1564 314 360 1013 245 1108
Fragments arranged by size:
1564 1108 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2785 2785
Size: 4604
NarI GG'CG_CC
Cuts at: 2035 2283 2035
Size: 248 4356
NcoI C'CATG_G
Cuts at: 107 107
Size: 4604
NgoMIV G'CCGG_C
Cuts at: 2783 2783
Size: 4604
NspI r_CATG'y
Cuts at: 1657 2157 3064 1657
Size: 500 907 3197
Fragments arranged by size:
3197 907 500
PciI A'CATG_T
Cuts at: 3060 3060
Size: 4604
PflMI CCAn_nnn'nTGG
Cuts at: 1493 1493
Size: 4604
PfoI T'CCnGG_A
Cuts at: 945 945
Size: 4604
PmeI GTTT'AAAC
Cuts at: 1603 1603
Size: 4604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 3743
Size: 32 4572
PstI C_TGCA'G
Cuts at: 1651 2336 1651
Size: 685 3919
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4604
PvuII CAG'CTG
Cuts at: 2029 2389 2029
Size: 360 4244
RsrII CG'GwC_CG
Cuts at: 2799 2799
Size: 4604
SacI G_AGCT'C
Cuts at: 1618 1618
Size: 4604
SacII CC_GC'GG
Cuts at: 152 152
Size: 4604
SalI G'TCGA_C
Cuts at: 1641 1641
Size: 4604
SapI GCTCTTCn'nnn_
Cuts at: 614 635 2627 2837 614
Size: 21 1992 210 2381
Fragments arranged by size:
2381 1992 210 21
SbfI CC_TGCA'GG
Cuts at: 1651 1651
Size: 4604
ScaI AGT'ACT
Cuts at: 243 4121 243
Size: 3878 726
SfcI C'TryA_G
Cuts at: 33 767 1647 2332 3325 3516 33
Size: 734 880 685 993 191 1121
Fragments arranged by size:
1121 993 880 734 685 191
SfoI GGC'GCC
Cuts at: 2036 2284 2036
Size: 248 4356
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
SmaI CCC'GGG
Cuts at: 1626 1626
Size: 4604
SmlI C'TyrA_G
Cuts at: 753 1759 2674 3166 3428 753
Size: 1006 915 492 262 1929
Fragments arranged by size:
1929 1006 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4604
SphI G_CATG'C
Cuts at: 1657 2157 1657
Size: 500 4104
StyI C'CwwG_G
Cuts at: 107 1738 2092 107
Size: 1631 354 2619
Fragments arranged by size:
2619 1631 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2187 2320 2779 2988 2187
Size: 133 459 209 3803
Fragments arranged by size:
3803 459 209 133
TatI w'GTAC_w
Cuts at: 241 4119 241
Size: 3878 726
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4121 4153 4121
Size: 32 4572
Tth111I GACn'n_nGTC
Cuts at: 2401 2401
Size: 4604
XbaI T'CTAG_A
Cuts at: 933 1005 1635 933
Size: 72 630 3902
Fragments arranged by size:
3902 630 72
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2099 2099
Size: 4604
XmaI C'CCGG_G
Cuts at: 1624 1624
Size: 4604
XmnI GAAnn'nnTTC
Cuts at: 739 929 941 739
Size: 190 12 4402
Fragments arranged by size:
4402 190 12
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI