Restriction map for
pF1KB9933
(Circular) (Six-base) MAPSORT of: pF1KB9933.seq Check: 9696 from: 1 to: 4580
pF1KB9933 4580 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 953 1618 953
Size: 665 3915
Acc65I G'GTAC_C
Cuts at: 1596 1596
Size: 4580
AclI AA'CG_TT
Cuts at: 4420 4420
Size: 4580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 807 1339 2405 2837 3584 3852 807
Size: 532 1066 432 747 268 1535
Fragments arranged by size:
1535 1066 747 532 432 268
AflIII A'CryG_T
Cuts at: 1031 1337 1932 3036 4013 1031
Size: 306 595 1104 977 1598
Fragments arranged by size:
1598 1104 977 595 306
AgeI A'CCGG_T
Cuts at: 2968 2968
Size: 4580
AleI CACnn'nnGTG
Cuts at: 736 946 736
Size: 210 4370
AlwNI CAG_nnn'CTG
Cuts at: 897 1485 1885 3452 897
Size: 588 400 1567 2025
Fragments arranged by size:
2025 1567 588 400
ApaI G_GGCC'C
Cuts at: 771 918 1217 1392 1494 771
Size: 147 299 175 102 3857
Fragments arranged by size:
3857 299 175 147 102
ApaLI G'TGCA_C
Cuts at: 3350 3350
Size: 4580
ApoI r'AATT_y
Cuts at: 139 1584 3933 4189 139
Size: 1445 2349 256 530
Fragments arranged by size:
2349 1445 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4580
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
AvaI C'yCGr_G
Cuts at: 871 903 1216 1368 1600 871
Size: 32 313 152 232 3851
Fragments arranged by size:
3851 313 232 152 32
AvrII C'CTAG_G
Cuts at: 1383 1383
Size: 4580
BamHI G'GATC_C
Cuts at: 1605 1605
Size: 4580
BanI G'GyrC_C
Cuts at: 280 527 730 1596 2010 2258 2293 280
Size: 247 203 866 414 248 35 2567
Fragments arranged by size:
2567 866 414 248 247 203 35
BanII G_rGCy'C
Cuts at: 771 918 1148 1217 1375 1392 1494 1594 2624
Size: 147 230 69 158 17 102 100 1030
Cuts at: 2624 771
Size: 2727
Fragments arranged by size:
2727 1030 230 158 147 102 100 69
17
BbeI G_GCGC'C
Cuts at: 2014 2262 2014
Size: 248 4332
BbvCI CC'TCA_GC
Cuts at: 1235 1235
Size: 4580
BciVI GTATCCnnnnn_n'
Cuts at: 2478 3239 4580 2478
Size: 761 1341 2478
Fragments arranged by size:
2478 1341 761
BclI T'GATC_A
Cuts at: 2100 2100
Size: 4580
BglI GCCn_nnn'nGGC
Cuts at: 1231 1453 1498 1231
Size: 222 45 4313
Fragments arranged by size:
4313 222 45
BglII A'GATC_T
Cuts at: 2095 4132 2095
Size: 2037 2543
BlpI GC'TnA_GC
Cuts at: 684 1692 684
Size: 1008 3572
Bme1580I G_kGCm'C
Cuts at: 350 500 598 733 771 918 1217 1301 1392
Size: 150 98 135 38 147 299 84 91
Cuts at: 1392 1494 2205 2298 3354 350
Size: 102 711 93 1056 1576
Fragments arranged by size:
1576 1056 711 299 150 147 135 102
98 93 91 84 38
BmrI ACTGGGnnnn_n'
Cuts at: 1792 1873 2208 4352 1792
Size: 81 335 2144 2020
Fragments arranged by size:
2144 2020 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 4580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 960 1108 1249 1486 1558 511
Size: 449 148 141 237 72 3533
Fragments arranged by size:
3533 449 237 148 141 72
Bpu10I CC'TnA_GC
Cuts at: 301 1235 301
Size: 934 3646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1756 2635 3127 3425 1756
Size: 879 492 298 2911
Fragments arranged by size:
2911 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4284 4284
Size: 4580
BsaAI yAC'GTr
Cuts at: 1296 1933 1296
Size: 637 3943
BsaBI GATnn'nnATC
Cuts at: 6 1273 2094 4131 6
Size: 1267 821 2037 455
Fragments arranged by size:
2037 1267 821 455
BsaHI Gr'CG_yC
Cuts at: 2011 2259 2961 4457 2011
Size: 248 702 1496 2134
Fragments arranged by size:
2134 1496 702 248
BsaWI w'CCGG_w
Cuts at: 983 1769 2290 2968 3242 3389 3800 983
Size: 786 521 678 274 147 411 1763
Fragments arranged by size:
1763 786 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 595 625 595
Size: 30 4550
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 619 1018 1252 1365 1483 1555 619
Size: 399 234 113 118 72 3644
Fragments arranged by size:
3644 399 234 118 113 72
BseYI C'CCAG_C
Cuts at: 1300 1426 1456 1548 2005 2763 3340 3836 1300
Size: 126 30 92 457 758 577 496 2044
Fragments arranged by size:
2044 758 577 496 457 126 92 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 578 613 405
Size: 173 35 4372
Fragments arranged by size:
4372 173 35
BsiEI CG_ry'CG
Cuts at: 104 1524 2122 2168 3376 104
Size: 1420 598 46 1208 1308
Fragments arranged by size:
1420 1308 1208 598 46
BsiHKAI G_wGCw'C
Cuts at: 296 1594 2372 2562 3354 296
Size: 1298 778 190 792 1522
Fragments arranged by size:
1522 1298 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 296 350 500 598 733 771 918 1148 1217
Size: 54 150 98 135 38 147 230 69
Cuts at: 1217 1301 1375 1392 1494 1594 2205 2298 2372
Size: 84 74 17 102 100 611 93 74
Cuts at: 2372 2562 2624 3354 296
Size: 190 62 730 1522
Fragments arranged by size:
1522 730 611 230 190 150 147 135
102 100 98 93 84 74 74 69
62 54 38 17
BspEI T'CCGG_A
Cuts at: 3800 3800
Size: 4580
BspHI T'CATG_A
Cuts at: 759 1154 3756 759
Size: 395 2602 1583
Fragments arranged by size:
2602 1583 395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1630 1844 2146 2527 1630
Size: 214 302 381 3683
Fragments arranged by size:
3683 381 302 214
BsrBI CCG'CTC
Cuts at: 50 747 840 1250 2872 2926 4410 4575 50
Size: 697 93 410 1622 54 1484 165 55
Fragments arranged by size:
1622 1484 697 410 165 93 55 54
BsrDI GCAATG_nn'
Cuts at: 2492 2492
Size: 4580
BsrFI r'CCGG_y
Cuts at: 162 477 1328 1352 2578 2759 2968 162
Size: 315 851 24 1226 181 209 1774
Fragments arranged by size:
1774 1226 851 315 209 181 24
BssSI C'ACGA_G
Cuts at: 2851 3209 2851
Size: 358 4222
BstAPI GCAn_nnn'nTGC
Cuts at: 227 1485 1957 227
Size: 1258 472 2850
Fragments arranged by size:
2850 1258 472
BstBI TT'CG_AA
Cuts at: 2941 2941
Size: 4580
BstXI CCAn_nnnn'nTGG
Cuts at: 249 510 792 249
Size: 261 282 4037
Fragments arranged by size:
4037 282 261
BstYI r'GATC_y
Cuts at: 1 440 1605 2072 2095 2430 2676 3688 3774
Size: 439 1165 467 23 335 246 1012 86
Cuts at: 3774 3786 4132 1
Size: 12 346 449
Fragments arranged by size:
1165 1012 467 449 439 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1495 1495
Size: 4580
BtgI C'CryG_G
Cuts at: 533 881 1361 533
Size: 348 480 3752
Fragments arranged by size:
3752 480 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 504 2530 2711 2874 504
Size: 2026 181 163 2210
Fragments arranged by size:
2210 2026 181 163
BtsI GCAGTG_nn'
Cuts at: 1279 1406 1823 1279
Size: 127 417 4036
Fragments arranged by size:
4036 417 127
DraI TTT'AAA
Cuts at: 1579 1579
Size: 4580
DrdI GACnn_nn'nnGTC
Cuts at: 424 2286 3144 424
Size: 1862 858 1860
Fragments arranged by size:
1862 1860 858
EaeI y'GGCC_r
Cuts at: 1439 2165 2339 2730 2757 4102 1439
Size: 726 174 391 27 1345 1917
Fragments arranged by size:
1917 1345 726 391 174 27
EagI C'GGCC_G
Cuts at: 2165 2165
Size: 4580
EarI CTCTTCn'nnn_
Cuts at: 1186 2603 2813 1186
Size: 1417 210 2953
Fragments arranged by size:
2953 1417 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2788 3098 3244 4390 2788
Size: 310 146 1146 2978
Fragments arranged by size:
2978 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1592 1592
Size: 4580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 807 960 1108 1249 1339 1486 1558 2405
Size: 296 153 148 141 90 147 72 847
Cuts at: 2405 2837 3584 3852 511
Size: 432 747 268 1239
Fragments arranged by size:
1239 847 747 432 296 268 153 148
147 141 90 72
EcoNI CCTnn'n_nnAGG
Cuts at: 1183 1183
Size: 4580
EcoO109I rG'GnC_Cy
Cuts at: 355 767 858 914 915 1083 1314 1388 1389
Size: 412 91 56 1 168 231 74 1
Cuts at: 1389 1491 1719 355
Size: 102 228 3216
Fragments arranged by size:
3216 412 231 228 168 102 91 74
56 1 1
EcoRI G'AATT_C
Cuts at: 1584 1584
Size: 4580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 4580
FspI TGC'GCA
Cuts at: 709 2361 709
Size: 1652 2928
FspAI rTGC'GCAy
Cuts at: 709 709
Size: 4580
HaeII r_GCGC'y
Cuts at: 853 1504 2014 2262 3284 4263 853
Size: 651 510 248 1022 979 1170
Fragments arranged by size:
1170 1022 979 651 510 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 500 532 2422 2452 2454 2484 500
Size: 32 1890 30 2 30 2596
Fragments arranged by size:
2596 1890 32 30 30 2
HincII GTy'rAC
Cuts at: 237 1619 237
Size: 1382 3198
KasI G'GCGC_C
Cuts at: 2010 2258 2010
Size: 248 4332
KpnI G_GTAC'C
Cuts at: 1600 1600
Size: 4580
MluI A'CGCG_T
Cuts at: 4013 4013
Size: 4580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1154 3251 3435 1154
Size: 2097 184 2299
Fragments arranged by size:
2299 2097 184
MscI TGG'CCA
Cuts at: 1441 2341 1441
Size: 900 3680
MslI CAynn'nnrTG
Cuts at: 196 427 736 946 1153 1159 2569 2696 196
Size: 231 309 210 207 6 1410 127 2080
Fragments arranged by size:
2080 1410 309 231 210 207 127 6
MspA1I CmG'CkG
Cuts at: 407 468 601 656 846 1056 1311 1430 1548
Size: 61 133 55 190 210 255 119 118
Cuts at: 1548 1691 2005 2365 3378 3623 407
Size: 143 314 360 1013 245 1364
Fragments arranged by size:
1364 1013 360 314 255 245 210 190
143 133 119 118 61 55
NaeI GCC'GGC
Cuts at: 1330 1354 2761 1330
Size: 24 1407 3149
Fragments arranged by size:
3149 1407 24
NarI GG'CG_CC
Cuts at: 2011 2259 2011
Size: 248 4332
NcoI C'CATG_G
Cuts at: 881 1361 881
Size: 480 4100
NgoMIV G'CCGG_C
Cuts at: 1328 1352 2759 1328
Size: 24 1407 3149
Fragments arranged by size:
3149 1407 24
NspI r_CATG'y
Cuts at: 151 692 1074 1633 2133 3040 151
Size: 541 382 559 500 907 1691
Fragments arranged by size:
1691 907 559 541 500 382
PciI A'CATG_T
Cuts at: 3036 3036
Size: 4580
PfoI T'CCnGG_A
Cuts at: 362 1184 362
Size: 822 3758
PmeI GTTT'AAAC
Cuts at: 1579 1579
Size: 4580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 3719
Size: 32 4548
PpuMI rG'GwC_Cy
Cuts at: 355 858 1314 355
Size: 503 456 3621
Fragments arranged by size:
3621 503 456
PshAI GACnn'nnGTC
Cuts at: 377 722 377
Size: 345 4235
PspOMI G'GGCC_C
Cuts at: 767 914 1213 1388 1490 767
Size: 147 299 175 102 3857
Fragments arranged by size:
3857 299 175 147 102
PspXI vC'TCGA_Gb
Cuts at: 871 903 871
Size: 32 4548
PstI C_TGCA'G
Cuts at: 893 1627 2312 893
Size: 734 685 3161
Fragments arranged by size:
3161 734 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4580
PvuII CAG'CTG
Cuts at: 656 846 1548 2005 2365 656
Size: 190 702 457 360 2871
Fragments arranged by size:
2871 702 457 360 190
RsrII CG'GwC_CG
Cuts at: 2775 2775
Size: 4580
SacI G_AGCT'C
Cuts at: 1594 1594
Size: 4580
SalI G'TCGA_C
Cuts at: 1617 1617
Size: 4580
SanDI GG'GwC_CC
Cuts at: 858 858
Size: 4580
SapI GCTCTTCn'nnn_
Cuts at: 2603 2813 2603
Size: 210 4370
SbfI CC_TGCA'GG
Cuts at: 893 1627 893
Size: 734 3846
ScaI AGT'ACT
Cuts at: 4097 4097
Size: 4580
SfcI C'TryA_G
Cuts at: 33 889 1623 2308 3301 3492 33
Size: 856 734 685 993 191 1121
Fragments arranged by size:
1121 993 856 734 685 191
SfoI GGC'GCC
Cuts at: 2012 2260 2012
Size: 248 4332
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
SmaI CCC'GGG
Cuts at: 1370 1602 1370
Size: 232 4348
SmlI C'TyrA_G
Cuts at: 871 903 1735 2650 3142 3404 871
Size: 32 832 915 492 262 2047
Fragments arranged by size:
2047 915 832 492 262 32
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4580
SphI G_CATG'C
Cuts at: 692 1074 1633 2133 692
Size: 382 559 500 3139
Fragments arranged by size:
3139 559 500 382
StuI AGG'CCT
Cuts at: 328 1291 328
Size: 963 3617
StyI C'CwwG_G
Cuts at: 881 1361 1383 1714 2068 881
Size: 480 22 331 354 3393
Fragments arranged by size:
3393 480 354 331 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1233 2163 2296 2755 2964 1233
Size: 930 133 459 209 2849
Fragments arranged by size:
2849 930 459 209 133
TatI w'GTAC_w
Cuts at: 4095 4095
Size: 4580
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4097 4129 4097
Size: 32 4548
Tth111I GACn'n_nGTC
Cuts at: 394 2377 394
Size: 1983 2597
XbaI T'CTAG_A
Cuts at: 1611 1611
Size: 4580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1083 1434 2075 1083
Size: 351 641 3588
Fragments arranged by size:
3588 641 351
XhoI C'TCGA_G
Cuts at: 871 903 871
Size: 32 4548
XmaI C'CCGG_G
Cuts at: 1368 1600 1368
Size: 232 4348
XmnI GAAnn'nnTTC
Cuts at: 1033 1033
Size: 4580
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PsiI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI