Restriction map for
pF1KB9909
(Circular) (Six-base) MAPSORT of: pF1KB9909.seq Check: 3644 from: 1 to: 3881
pF1KB9909 3881 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 919 919
Size: 3881
Acc65I G'GTAC_C
Cuts at: 897 897
Size: 3881
AclI AA'CG_TT
Cuts at: 3721 3721
Size: 3881
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1706 2138 2885 3153 1706
Size: 432 747 268 2434
Fragments arranged by size:
2434 747 432 268
AflIII A'CryG_T
Cuts at: 1233 2337 3314 1233
Size: 1104 977 1800
Fragments arranged by size:
1800 1104 977
AgeI A'CCGG_T
Cuts at: 2269 2269
Size: 3881
AlwNI CAG_nnn'CTG
Cuts at: 345 564 615 1186 2753 345
Size: 219 51 571 1567 1473
Fragments arranged by size:
1567 1473 571 219 51
ApaLI G'TGCA_C
Cuts at: 2651 2651
Size: 3881
ApoI r'AATT_y
Cuts at: 885 3234 3490 885
Size: 2349 256 1276
Fragments arranged by size:
2349 1276 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3881
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
AvaI C'yCGr_G
Cuts at: 176 258 901 176
Size: 82 643 3156
Fragments arranged by size:
3156 643 82
BamHI G'GATC_C
Cuts at: 833 906 833
Size: 73 3808
BanI G'GyrC_C
Cuts at: 280 553 589 897 1311 1559 1594 280
Size: 273 36 308 414 248 35 2567
Fragments arranged by size:
2567 414 308 273 248 36 35
BanII G_rGCy'C
Cuts at: 369 392 626 895 1925 369
Size: 23 234 269 1030 2325
Fragments arranged by size:
2325 1030 269 234 23
BbeI G_GCGC'C
Cuts at: 1315 1563 1315
Size: 248 3633
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 687 721 687
Size: 34 3847
BciVI GTATCCnnnnn_n'
Cuts at: 1779 2540 3881 1779
Size: 761 1341 1779
Fragments arranged by size:
1779 1341 761
BclI T'GATC_A
Cuts at: 1401 1401
Size: 3881
BglII A'GATC_T
Cuts at: 1396 3433 1396
Size: 2037 1844
BlpI GC'TnA_GC
Cuts at: 163 478 993 163
Size: 315 515 3051
Fragments arranged by size:
3051 515 315
Bme1580I G_kGCm'C
Cuts at: 203 548 594 1506 1599 2655 203
Size: 345 46 912 93 1056 1429
Fragments arranged by size:
1429 1056 912 345 93 46
BmrI ACTGGGnnnn_n'
Cuts at: 552 1093 1174 1509 3653 552
Size: 541 81 335 2144 780
Fragments arranged by size:
2144 780 541 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 3881
Bpu10I CC'TnA_GC
Cuts at: 416 416
Size: 3881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1936 2428 2726 1057
Size: 879 492 298 2212
Fragments arranged by size:
2212 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3585 3585
Size: 3881
BsaAI yAC'GTr
Cuts at: 825 1234 825
Size: 409 3472
BsaBI GATnn'nnATC
Cuts at: 6 1395 3432 6
Size: 1389 2037 455
Fragments arranged by size:
2037 1389 455
BsaHI Gr'CG_yC
Cuts at: 139 1312 1560 2262 3758 139
Size: 1173 248 702 1496 262
Fragments arranged by size:
1496 1173 702 262 248
BsaWI w'CCGG_w
Cuts at: 1070 1591 2269 2543 2690 3101 1070
Size: 521 678 274 147 411 1850
Fragments arranged by size:
1850 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 220 250 220
Size: 30 3851
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 457
Size: 3881
BseYI C'CCAG_C
Cuts at: 124 295 338 395 486 575 603 1306 2064
Size: 171 43 57 91 89 28 703 758
Cuts at: 2064 2641 3137 124
Size: 577 496 868
Fragments arranged by size:
868 758 703 577 496 171 91 89
57 43 28
BsiEI CG_ry'CG
Cuts at: 104 314 1423 1469 2677 104
Size: 210 1109 46 1208 1308
Fragments arranged by size:
1308 1208 1109 210 46
BsiHKAI G_wGCw'C
Cuts at: 362 626 895 1673 1863 2655 362
Size: 264 269 778 190 792 1588
Fragments arranged by size:
1588 792 778 269 264 190
Bsp1286I G_dGCh'C
Cuts at: 203 362 369 392 548 594 626 895 1506
Size: 159 7 23 156 46 32 269 611
Cuts at: 1506 1599 1673 1863 1925 2655 203
Size: 93 74 190 62 730 1429
Fragments arranged by size:
1429 730 611 269 190 159 156 93
74 62 46 32 23 7
BspEI T'CCGG_A
Cuts at: 3101 3101
Size: 3881
BspHI T'CATG_A
Cuts at: 3057 3057
Size: 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 931 1145 1447 1828 931
Size: 214 302 381 2984
Fragments arranged by size:
2984 381 302 214
BsrBI CCG'CTC
Cuts at: 50 167 2173 2227 3711 3876 50
Size: 117 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 117 55 54
BsrDI GCAATG_nn'
Cuts at: 1793 1793
Size: 3881
BsrFI r'CCGG_y
Cuts at: 116 653 694 1879 2060 2269 116
Size: 537 41 1185 181 209 1728
Fragments arranged by size:
1728 1185 537 209 181 41
BssSI C'ACGA_G
Cuts at: 202 2152 2510 202
Size: 1950 358 1573
Fragments arranged by size:
1950 1573 358
BstAPI GCAn_nnn'nTGC
Cuts at: 324 560 1258 324
Size: 236 698 2947
Fragments arranged by size:
2947 698 236
BstBI TT'CG_AA
Cuts at: 2242 2242
Size: 3881
BstEII G'GTnAC_C
Cuts at: 639 639
Size: 3881
BstYI r'GATC_y
Cuts at: 1 833 906 1373 1396 1731 1977 2989 3075
Size: 832 73 467 23 335 246 1012 86
Cuts at: 3075 3087 3433 1
Size: 12 346 449
Fragments arranged by size:
1012 832 467 449 346 335 246 86
73 23 12
BtgI C'CryG_G
Cuts at: 107 549 569 635 107
Size: 442 20 66 3353
Fragments arranged by size:
3353 442 66 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1831 2012 2175 1831
Size: 181 163 3537
Fragments arranged by size:
3537 181 163
BtsI GCAGTG_nn'
Cuts at: 318 1124 318
Size: 806 3075
DraI TTT'AAA
Cuts at: 880 880
Size: 3881
DraIII CAC_nnn'GTG
Cuts at: 375 560 375
Size: 185 3696
DrdI GACnn_nn'nnGTC
Cuts at: 1587 2445 1587
Size: 858 3023
EaeI y'GGCC_r
Cuts at: 311 566 655 1466 1640 2031 2058 3403 311
Size: 255 89 811 174 391 27 1345 789
Fragments arranged by size:
1345 811 789 391 255 174 89 27
EagI C'GGCC_G
Cuts at: 311 1466 311
Size: 1155 2726
EarI CTCTTCn'nnn_
Cuts at: 1904 2114 1904
Size: 210 3671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2089 2399 2545 3691 2089
Size: 310 146 1146 2279
Fragments arranged by size:
2279 1146 310 146
EcoICRI GAG'CTC
Cuts at: 624 893 624
Size: 269 3612
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1706 2138 2885 3153 1706
Size: 432 747 268 2434
Fragments arranged by size:
2434 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 612 1020 612
Size: 408 3473
EcoRI G'AATT_C
Cuts at: 885 885
Size: 3881
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1900 1900
Size: 3881
FspI TGC'GCA
Cuts at: 1662 1662
Size: 3881
HaeII r_GCGC'y
Cuts at: 345 1315 1563 2585 3564 345
Size: 970 248 1022 979 662
Fragments arranged by size:
1022 979 970 662 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1723 1753 1755 1785 1723
Size: 30 2 30 3819
Fragments arranged by size:
3819 30 30 2
HincII GTy'rAC
Cuts at: 920 920
Size: 3881
KasI G'GCGC_C
Cuts at: 1311 1559 1311
Size: 248 3633
KpnI G_GTAC'C
Cuts at: 901 901
Size: 3881
MluI A'CGCG_T
Cuts at: 3314 3314
Size: 3881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2552 2736 2552
Size: 184 3697
MscI TGG'CCA
Cuts at: 568 1642 568
Size: 1074 2807
MslI CAynn'nnrTG
Cuts at: 1870 1997 1870
Size: 127 3754
MspA1I CmG'CkG
Cuts at: 477 522 992 1306 1666 2679 2924 477
Size: 45 470 314 360 1013 245 1434
Fragments arranged by size:
1434 1013 470 360 314 245 45
NaeI GCC'GGC
Cuts at: 655 696 2062 655
Size: 41 1366 2474
Fragments arranged by size:
2474 1366 41
NarI GG'CG_CC
Cuts at: 1312 1560 1312
Size: 248 3633
NcoI C'CATG_G
Cuts at: 107 569 107
Size: 462 3419
NgoMIV G'CCGG_C
Cuts at: 653 694 2060 653
Size: 41 1366 2474
Fragments arranged by size:
2474 1366 41
NspI r_CATG'y
Cuts at: 934 1434 2341 934
Size: 500 907 2474
Fragments arranged by size:
2474 907 500
PciI A'CATG_T
Cuts at: 2337 2337
Size: 3881
PflMI CCAn_nnn'nTGG
Cuts at: 345 564 575 345
Size: 219 11 3651
Fragments arranged by size:
3651 219 11
PmeI GTTT'AAAC
Cuts at: 880 880
Size: 3881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 3849
PpuMI rG'GwC_Cy
Cuts at: 612 612
Size: 3881
PstI C_TGCA'G
Cuts at: 245 620 928 1613 245
Size: 375 308 685 2513
Fragments arranged by size:
2513 685 375 308
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3881
PvuII CAG'CTG
Cuts at: 477 522 1306 1666 477
Size: 45 784 360 2692
Fragments arranged by size:
2692 784 360 45
RsrII CG'GwC_CG
Cuts at: 2076 2076
Size: 3881
SacI G_AGCT'C
Cuts at: 626 895 626
Size: 269 3612
SalI G'TCGA_C
Cuts at: 918 918
Size: 3881
SapI GCTCTTCn'nnn_
Cuts at: 1904 2114 1904
Size: 210 3671
SbfI CC_TGCA'GG
Cuts at: 620 928 620
Size: 308 3573
ScaI AGT'ACT
Cuts at: 3398 3398
Size: 3881
SfcI C'TryA_G
Cuts at: 33 241 616 924 1609 2602 2793 33
Size: 208 375 308 685 993 191 1121
Fragments arranged by size:
1121 993 685 375 308 208 191
SfoI GGC'GCC
Cuts at: 1313 1561 1313
Size: 248 3633
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
SmaI CCC'GGG
Cuts at: 260 903 260
Size: 643 3238
SmlI C'TyrA_G
Cuts at: 1036 1951 2443 2705 1036
Size: 915 492 262 2212
Fragments arranged by size:
2212 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3881
SphI G_CATG'C
Cuts at: 934 1434 934
Size: 500 3381
StyI C'CwwG_G
Cuts at: 107 569 747 863 1015 1369 107
Size: 462 178 116 152 354 2619
Fragments arranged by size:
2619 462 354 178 152 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 409 571 577 1464 1597 2056 2265 409
Size: 162 6 887 133 459 209 2025
Fragments arranged by size:
2025 887 459 209 162 133 6
TatI w'GTAC_w
Cuts at: 3396 3396
Size: 3881
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 3849
Tth111I GACn'n_nGTC
Cuts at: 1678 1678
Size: 3881
XbaI T'CTAG_A
Cuts at: 912 912
Size: 3881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 1376 132
Size: 1244 2637
XmaI C'CCGG_G
Cuts at: 258 901 258
Size: 643 3238
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI BpmI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI