Restriction map for
pF1KB9906
(Circular) (Six-base) MAPSORT of: pF1KB9906.seq Check: 7139 from: 1 to: 4880
pF1KB9906 4880 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1918 1918
Size: 4880
Acc65I G'GTAC_C
Cuts at: 1896 1896
Size: 4880
AclI AA'CG_TT
Cuts at: 309 820 4720 309
Size: 511 3900 469
Fragments arranged by size:
3900 511 469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1681 2705 3137 3884 4152 1681
Size: 1024 432 747 268 2409
Fragments arranged by size:
2409 1024 747 432 268
AflIII A'CryG_T
Cuts at: 1286 2232 3336 4313 1286
Size: 946 1104 977 1853
Fragments arranged by size:
1853 1104 977 946
AgeI A'CCGG_T
Cuts at: 3268 3268
Size: 4880
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 395 427 395
Size: 32 4848
AlwNI CAG_nnn'CTG
Cuts at: 2185 3752 2185
Size: 1567 3313
ApaLI G'TGCA_C
Cuts at: 3650 3650
Size: 4880
ApoI r'AATT_y
Cuts at: 253 356 392 1191 1884 4233 4489 253
Size: 103 36 799 693 2349 256 644
Fragments arranged by size:
2349 799 693 644 256 103 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 4880
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
AvaI C'yCGr_G
Cuts at: 265 1539 1900 265
Size: 1274 361 3245
Fragments arranged by size:
3245 1274 361
BamHI G'GATC_C
Cuts at: 1905 1905
Size: 4880
BanI G'GyrC_C
Cuts at: 178 1197 1896 2310 2558 2593 178
Size: 1019 699 414 248 35 2465
Fragments arranged by size:
2465 1019 699 414 248 35
BanII G_rGCy'C
Cuts at: 145 209 1083 1864 1894 2924 145
Size: 64 874 781 30 1030 2101
Fragments arranged by size:
2101 1030 874 781 64 30
BbeI G_GCGC'C
Cuts at: 182 2314 2562 182
Size: 2132 248 2500
Fragments arranged by size:
2500 2132 248
BbsI GAAGACnn'nnnn_
Cuts at: 238 238
Size: 4880
BbvCI CC'TCA_GC
Cuts at: 1391 1721 1391
Size: 330 4550
BciVI GTATCCnnnnn_n'
Cuts at: 2778 3539 4880 2778
Size: 761 1341 2778
Fragments arranged by size:
2778 1341 761
BclI T'GATC_A
Cuts at: 2400 2400
Size: 4880
BglI GCCn_nnn'nGGC
Cuts at: 187 187
Size: 4880
BglII A'GATC_T
Cuts at: 2395 4432 2395
Size: 2037 2843
BlpI GC'TnA_GC
Cuts at: 458 1992 458
Size: 1534 3346
Bme1580I G_kGCm'C
Cuts at: 266 2505 2598 3654 266
Size: 2239 93 1056 1492
Fragments arranged by size:
2239 1492 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1867 2092 2173 2508 4652 1867
Size: 225 81 335 2144 2095
Fragments arranged by size:
2144 2095 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 291 3247 291
Size: 2956 1924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1223 1610 1730 1223
Size: 387 120 4373
Fragments arranged by size:
4373 387 120
Bpu10I CC'TnA_GC
Cuts at: 1391 1721 1391
Size: 330 4550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 2056 2935 3427 3725 399
Size: 1657 879 492 298 1554
Fragments arranged by size:
1657 1554 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4584 4584
Size: 4880
BsaAI yAC'GTr
Cuts at: 2233 2233
Size: 4880
BsaBI GATnn'nnATC
Cuts at: 6 2394 4431 6
Size: 2388 2037 455
Fragments arranged by size:
2388 2037 455
BsaHI Gr'CG_yC
Cuts at: 179 2311 2559 3261 4757 179
Size: 2132 248 702 1496 302
Fragments arranged by size:
2132 1496 702 302 248
BsaWI w'CCGG_w
Cuts at: 2069 2590 3268 3542 3689 4100 2069
Size: 521 678 274 147 411 2849
Fragments arranged by size:
2849 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1403 1433 1403
Size: 30 4850
BseYI C'CCAG_C
Cuts at: 1865 2305 3063 3640 4136 1865
Size: 440 758 577 496 2609
Fragments arranged by size:
2609 758 577 496 440
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 950 1334 950
Size: 384 4496
BsiEI CG_ry'CG
Cuts at: 104 191 2422 2468 3676 104
Size: 87 2231 46 1208 1308
Fragments arranged by size:
2231 1308 1208 87 46
BsiHKAI G_wGCw'C
Cuts at: 1894 2672 2862 3654 1894
Size: 778 190 792 3120
Fragments arranged by size:
3120 792 778 190
BsmI GAATG_Cn'
Cuts at: 1241 1241
Size: 4880
Bsp1286I G_dGCh'C
Cuts at: 145 209 266 1083 1864 1894 2505 2598 2672
Size: 64 57 817 781 30 611 93 74
Cuts at: 2672 2862 2924 3654 145
Size: 190 62 730 1371
Fragments arranged by size:
1371 817 781 730 611 190 93 74
64 62 57 30
BspEI T'CCGG_A
Cuts at: 4100 4100
Size: 4880
BspHI T'CATG_A
Cuts at: 215 396 4056 215
Size: 181 3660 1039
Fragments arranged by size:
3660 1039 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 565 1930 2144 2446 2827 565
Size: 1365 214 302 381 2618
Fragments arranged by size:
2618 1365 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3172 3226 4710 4875 50
Size: 3122 54 1484 165 55
Fragments arranged by size:
3122 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 489 851 1472 1490 1781 2792 489
Size: 362 621 18 291 1011 2577
Fragments arranged by size:
2577 1011 621 362 291 18
BsrFI r'CCGG_y
Cuts at: 2878 3059 3268 2878
Size: 181 209 4490
Fragments arranged by size:
4490 209 181
BsrGI T'GTAC_A
Cuts at: 932 932
Size: 4880
BssSI C'ACGA_G
Cuts at: 3151 3509 3151
Size: 358 4522
BstAPI GCAn_nnn'nTGC
Cuts at: 2257 2257
Size: 4880
BstBI TT'CG_AA
Cuts at: 3241 3241
Size: 4880
BstXI CCAn_nnnn'nTGG
Cuts at: 1131 1131
Size: 4880
BstYI r'GATC_y
Cuts at: 1 1059 1612 1905 2372 2395 2730 2976 3988
Size: 1058 553 293 467 23 335 246 1012
Cuts at: 3988 4074 4086 4432 1
Size: 86 12 346 449
Fragments arranged by size:
1058 1012 553 467 449 346 335 293
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 443 443
Size: 4880
BtgI C'CryG_G
Cuts at: 107 185 200 1075 107
Size: 78 15 875 3912
Fragments arranged by size:
3912 875 78 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2830 3011 3174 2830
Size: 181 163 4536
Fragments arranged by size:
4536 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 2123 1118
Size: 1005 3875
DraI TTT'AAA
Cuts at: 315 1879 315
Size: 1564 3316
DraIII CAC_nnn'GTG
Cuts at: 1630 1630
Size: 4880
DrdI GACnn_nn'nnGTC
Cuts at: 575 2586 3444 575
Size: 2011 858 2011
Fragments arranged by size:
2011 2011 858
EaeI y'GGCC_r
Cuts at: 110 188 737 2465 2639 3030 3057 4402 110
Size: 78 549 1728 174 391 27 1345 588
Fragments arranged by size:
1728 1345 588 549 391 174 78 27
EagI C'GGCC_G
Cuts at: 188 2465 188
Size: 2277 2603
EarI CTCTTCn'nnn_
Cuts at: 1602 2903 3113 1602
Size: 1301 210 3369
Fragments arranged by size:
3369 1301 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3088 3398 3544 4690 3088
Size: 310 146 1146 3278
Fragments arranged by size:
3278 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1892 1892
Size: 4880
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1223 1610 1681 1730 2705 3137 3884 4152 1223
Size: 387 71 49 975 432 747 268 1951
Fragments arranged by size:
1951 975 747 432 387 268 71 49
EcoNI CCTnn'n_nnAGG
Cuts at: 1205 1205
Size: 4880
EcoO109I rG'GnC_Cy
Cuts at: 2019 2019
Size: 4880
EcoRI G'AATT_C
Cuts at: 253 392 1884 253
Size: 139 1492 3249
Fragments arranged by size:
3249 1492 139
EcoRV GAT'ATC
Cuts at: 1833 1833
Size: 4880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 4880
FspI TGC'GCA
Cuts at: 2661 2661
Size: 4880
HaeII r_GCGC'y
Cuts at: 158 182 2314 2562 3584 4563 158
Size: 24 2132 248 1022 979 475
Fragments arranged by size:
2132 1022 979 475 248 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1403 1435 1444 1476 1817 1849 2722 2752 2754
Size: 32 9 32 341 32 873 30 2
Cuts at: 2754 2784 1403
Size: 30 3499
Fragments arranged by size:
3499 873 341 32 32 32 30 30
9 2
HincII GTy'rAC
Cuts at: 1036 1919 1036
Size: 883 3997
KasI G'GCGC_C
Cuts at: 178 2310 2558 178
Size: 2132 248 2500
Fragments arranged by size:
2500 2132 248
KpnI G_GTAC'C
Cuts at: 1900 1900
Size: 4880
MluI A'CGCG_T
Cuts at: 4313 4313
Size: 4880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 803 1458 3551 3735 803
Size: 655 2093 184 1948
Fragments arranged by size:
2093 1948 655 184
MscI TGG'CCA
Cuts at: 739 2641 739
Size: 1902 2978
MslI CAynn'nnrTG
Cuts at: 2869 2996 2869
Size: 127 4753
MspA1I CmG'CkG
Cuts at: 187 193 202 475 1115 1991 2305 2665 3678
Size: 6 9 273 640 876 314 360 1013
Cuts at: 3678 3923 187
Size: 245 1144
Fragments arranged by size:
1144 1013 876 640 360 314 273 245
9 6
NaeI GCC'GGC
Cuts at: 3061 3061
Size: 4880
NarI GG'CG_CC
Cuts at: 179 2311 2559 179
Size: 2132 248 2500
Fragments arranged by size:
2500 2132 248
NcoI C'CATG_G
Cuts at: 107 1075 107
Size: 968 3912
NdeI CA'TA_TG
Cuts at: 788 788
Size: 4880
NgoMIV G'CCGG_C
Cuts at: 3059 3059
Size: 4880
NotI GC'GGCC_GC
Cuts at: 188 188
Size: 4880
NspI r_CATG'y
Cuts at: 702 933 1290 1933 2433 3340 702
Size: 231 357 643 500 907 2242
Fragments arranged by size:
2242 907 643 500 357 231
PasI CC'CwG_GG
Cuts at: 904 1552 904
Size: 648 4232
PciI A'CATG_T
Cuts at: 1286 3336 1286
Size: 2050 2830
PflMI CCAn_nnn'nTGG
Cuts at: 675 1076 675
Size: 401 4479
PmeI GTTT'AAAC
Cuts at: 1879 1879
Size: 4880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 4848
PstI C_TGCA'G
Cuts at: 1927 2612 1927
Size: 685 4195
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4880
PvuII CAG'CTG
Cuts at: 475 2305 2665 475
Size: 1830 360 2690
Fragments arranged by size:
2690 1830 360
RsrII CG'GwC_CG
Cuts at: 3075 3075
Size: 4880
SacI G_AGCT'C
Cuts at: 1894 1894
Size: 4880
SacII CC_GC'GG
Cuts at: 188 203 188
Size: 15 4865
SalI G'TCGA_C
Cuts at: 1917 1917
Size: 4880
SapI GCTCTTCn'nnn_
Cuts at: 1602 2903 3113 1602
Size: 1301 210 3369
Fragments arranged by size:
3369 1301 210
SbfI CC_TGCA'GG
Cuts at: 1927 1927
Size: 4880
ScaI AGT'ACT
Cuts at: 1152 1179 4397 1152
Size: 27 3218 1635
Fragments arranged by size:
3218 1635 27
SfcI C'TryA_G
Cuts at: 33 619 1726 1923 2608 3601 3792 33
Size: 586 1107 197 685 993 191 1121
Fragments arranged by size:
1121 1107 993 685 586 197 191
SfoI GGC'GCC
Cuts at: 180 2312 2560 180
Size: 2132 248 2500
Fragments arranged by size:
2500 2132 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
SmaI CCC'GGG
Cuts at: 1541 1902 1541
Size: 361 4519
SmlI C'TyrA_G
Cuts at: 378 2035 2950 3442 3704 378
Size: 1657 915 492 262 1554
Fragments arranged by size:
1657 1554 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4880
SphI G_CATG'C
Cuts at: 702 1933 2433 702
Size: 1231 500 3149
Fragments arranged by size:
3149 1231 500
StuI AGG'CCT
Cuts at: 441 1492 441
Size: 1051 3829
StyI C'CwwG_G
Cuts at: 107 561 1001 1075 2014 2368 107
Size: 454 440 74 939 354 2619
Fragments arranged by size:
2619 939 454 440 354 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 890 1123 2463 2596 3055 3264 890
Size: 233 1340 133 459 209 2506
Fragments arranged by size:
2506 1340 459 233 209 133
TatI w'GTAC_w
Cuts at: 932 1150 1177 4395 932
Size: 218 27 3218 1417
Fragments arranged by size:
3218 1417 218 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4397 4429 4397
Size: 32 4848
Tth111I GACn'n_nGTC
Cuts at: 2677 2677
Size: 4880
XbaI T'CTAG_A
Cuts at: 1911 1911
Size: 4880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2375 2375
Size: 4880
XmaI C'CCGG_G
Cuts at: 1539 1900 1539
Size: 361 4519
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NspI PasI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BseRI
BsiWI BsmBI BssHII BstEII BstZ17I ClaI CspCI FseI
FspAI HindIII HpaI MfeI NheI NruI NsiI PacI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI