Restriction map for
pF1KB9898
(Circular) (Six-base) MAPSORT of: pF1KB9898.seq Check: 2720 from: 1 to: 9119
pF1KB9898 9119 bp
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3153 3153
Size: 9119
AccI GT'mk_AC
Cuts at: 5121 6157 5121
Size: 1036 8083
Acc65I G'GTAC_C
Cuts at: 6135 6135
Size: 9119
AclI AA'CG_TT
Cuts at: 8959 8959
Size: 9119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 628 1349 1750 1861 2213 2771 3223 3353
Size: 216 721 401 111 352 558 452 130
Cuts at: 3353 4213 4847 5969 6944 7376 8123 8391 412
Size: 860 634 1122 975 432 747 268 1140
Fragments arranged by size:
1140 1122 975 860 747 721 634 558
452 432 401 352 268 216 130 111
AflIII A'CryG_T
Cuts at: 1509 6471 7575 8552 1509
Size: 4962 1104 977 2076
Fragments arranged by size:
4962 2076 1104 977
AgeI A'CCGG_T
Cuts at: 7507 7507
Size: 9119
AhdI GACnn_n'nnGTC
Cuts at: 5106 5106
Size: 9119
AleI CACnn'nnGTG
Cuts at: 3767 4176 3767
Size: 409 8710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1201 4194 1201
Size: 2993 6126
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4885 4917 4885
Size: 32 9087
AlwNI CAG_nnn'CTG
Cuts at: 651 748 2133 2236 3201 3933 4446 4774 6424
Size: 97 1385 103 965 732 513 328 1650
Cuts at: 6424 7991 651
Size: 1567 1779
Fragments arranged by size:
1779 1650 1567 1385 965 732 513 328
103 97
ApaLI G'TGCA_C
Cuts at: 2086 7889 2086
Size: 5803 3316
ApoI r'AATT_y
Cuts at: 1159 1929 2553 3459 4321 4935 6123 8472 8728
Size: 770 624 906 862 614 1188 2349 256
Cuts at: 8728 1159
Size: 1550
Fragments arranged by size:
2349 1550 1188 906 862 770 624 614
256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9119
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9119
AvaI C'yCGr_G
Cuts at: 6083 6139 6083
Size: 56 9063
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1990 2023 3461 3494 1990
Size: 33 1438 33 7615
Fragments arranged by size:
7615 1438 33 33
BamHI G'GATC_C
Cuts at: 3936 4798 5673 6144 3936
Size: 862 875 471 6911
Fragments arranged by size:
6911 875 862 471
BanI G'GyrC_C
Cuts at: 1476 3952 4107 4432 6135 6549 6797 6832 1476
Size: 2476 155 325 1703 414 248 35 3763
Fragments arranged by size:
3763 2476 1703 414 325 248 155 35
BanII G_rGCy'C
Cuts at: 240 425 1952 5993 6133 7163 240
Size: 185 1527 4041 140 1030 2196
Fragments arranged by size:
4041 2196 1527 1030 185 140
BbeI G_GCGC'C
Cuts at: 6553 6801 6553
Size: 248 8871
BbsI GAAGACnn'nnnn_
Cuts at: 4316 4316
Size: 9119
BbvCI CC'TCA_GC
Cuts at: 1355 3862 1355
Size: 2507 6612
BciVI GTATCCnnnnn_n'
Cuts at: 3462 5099 7017 7778 9119 3462
Size: 1637 1918 761 1341 3462
Fragments arranged by size:
3462 1918 1637 1341 761
BclI T'GATC_A
Cuts at: 2409 6639 2409
Size: 4230 4889
BfrBI ATG'CAT
Cuts at: 4138 4138
Size: 9119
BglI GCCn_nnn'nGGC
Cuts at: 1947 5869 1947
Size: 3922 5197
BglII A'GATC_T
Cuts at: 1758 2736 6634 8671 1758
Size: 978 3898 2037 2206
Fragments arranged by size:
3898 2206 2037 978
BlpI GC'TnA_GC
Cuts at: 2852 5969 6231 2852
Size: 3117 262 5740
Fragments arranged by size:
5740 3117 262
Bme1580I G_kGCm'C
Cuts at: 1769 2090 6744 6837 7893 1769
Size: 321 4654 93 1056 2995
Fragments arranged by size:
4654 2995 1056 321 93
BmgBI CAC'GTC
Cuts at: 4383 4383
Size: 9119
BmrI ACTGGGnnnn_n'
Cuts at: 2191 5055 6331 6412 6747 8891 2191
Size: 2864 1276 81 335 2144 2419
Fragments arranged by size:
2864 2419 2144 1276 335 81
BmtI G_CTAG'C
Cuts at: 1147 1147
Size: 9119
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4578 7486 4578
Size: 2908 6211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 2008 2044 2362 2545 4093 4573 193
Size: 1815 36 318 183 1548 480 4739
Fragments arranged by size:
4739 1815 1548 480 318 183 36
Bpu10I CC'TnA_GC
Cuts at: 1355 3862 4409 1355
Size: 2507 547 6065
Fragments arranged by size:
6065 2507 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1627 4898 6295 7174 7666 7964 1627
Size: 3271 1397 879 492 298 2782
Fragments arranged by size:
3271 2782 1397 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3178 4728 8823 3178
Size: 1550 4095 3474
Fragments arranged by size:
4095 3474 1550
BsaAI yAC'GTr
Cuts at: 4600 6472 4600
Size: 1872 7247
BsaBI GATnn'nnATC
Cuts at: 6 3793 6633 8670 6
Size: 3787 2840 2037 455
Fragments arranged by size:
3787 2840 2037 455
BsaHI Gr'CG_yC
Cuts at: 6550 6798 7500 8996 6550
Size: 248 702 1496 6673
Fragments arranged by size:
6673 1496 702 248
BsaWI w'CCGG_w
Cuts at: 6308 6829 7507 7781 7928 8339 6308
Size: 521 678 274 147 411 7088
Fragments arranged by size:
7088 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1981 2011 2758 2788 5017 5047 5681 5711 6051
Size: 30 747 30 2229 30 634 30 340
Cuts at: 6051 6081 1981
Size: 30 5019
Fragments arranged by size:
5019 2229 747 634 340 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1325 1328 4429 1325
Size: 3 3101 6015
Fragments arranged by size:
6015 3101 3
BseYI C'CCAG_C
Cuts at: 3342 4682 4691 4721 4739 6544 7302 7879 8375
Size: 1340 9 30 18 1805 758 577 496
Cuts at: 8375 3342
Size: 4086
Fragments arranged by size:
4086 1805 1340 758 577 496 30 18
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 2146 3184 5429 5645 853
Size: 1293 1038 2245 216 4327
Fragments arranged by size:
4327 2245 1293 1038 216
BsiEI CG_ry'CG
Cuts at: 104 6661 6707 7915 104
Size: 6557 46 1208 1308
Fragments arranged by size:
6557 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 240 1994 2090 4173 4245 6133 6911 7101 7893
Size: 1754 96 2083 72 1888 778 190 792
Cuts at: 7893 240
Size: 1466
Fragments arranged by size:
2083 1888 1754 1466 792 778 190 96
72
BsmBI CGTCTCn'nnnn_
Cuts at: 3850 4812 3850
Size: 962 8157
Bsp1286I G_dGCh'C
Cuts at: 240 425 1769 1952 1994 2090 4173 4245 5993
Size: 185 1344 183 42 96 2083 72 1748
Cuts at: 5993 6133 6744 6837 6911 7101 7163 7893 240
Size: 140 611 93 74 190 62 730 1466
Fragments arranged by size:
2083 1748 1466 1344 730 611 190 185
183 140 96 93 74 72 62 42
BspEI T'CCGG_A
Cuts at: 8339 8339
Size: 9119
BspHI T'CATG_A
Cuts at: 5286 8295 5286
Size: 3009 6110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3153 3556 4912 6008 6169 6383 6685 7066 3153
Size: 403 1356 1096 161 214 302 381 5206
Fragments arranged by size:
5206 1356 1096 403 381 302 214 161
BsrBI CCG'CTC
Cuts at: 50 7411 7465 8949 9114 50
Size: 7361 54 1484 165 55
Fragments arranged by size:
7361 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 824 1040 5013 7031 824
Size: 216 3973 2018 2912
Fragments arranged by size:
3973 2912 2018 216
BsrFI r'CCGG_y
Cuts at: 3890 7117 7298 7507 3890
Size: 3227 181 209 5502
Fragments arranged by size:
5502 3227 209 181
BsrGI T'GTAC_A
Cuts at: 5250 5250
Size: 9119
BssSI C'ACGA_G
Cuts at: 1754 7390 7748 1754
Size: 5636 358 3125
Fragments arranged by size:
5636 3125 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2700 4135 4269 6496 2700
Size: 1435 134 2227 5323
Fragments arranged by size:
5323 2227 1435 134
BstBI TT'CG_AA
Cuts at: 7480 7480
Size: 9119
BstXI CCAn_nnnn'nTGG
Cuts at: 4104 4104
Size: 9119
BstYI r'GATC_y
Cuts at: 1 1758 2736 3936 4019 4798 5076 5673 5795
Size: 1757 978 1200 83 779 278 597 122
Cuts at: 5795 6144 6611 6634 6969 7215 8227 8313 8325
Size: 349 467 23 335 246 1012 86 12
Cuts at: 8325 8671 1
Size: 346 449
Fragments arranged by size:
1757 1200 1012 978 779 597 467 449
349 346 335 278 246 122 86 83
23 12
Bsu36I CC'TnA_GG
Cuts at: 918 1472 4337 918
Size: 554 2865 5700
Fragments arranged by size:
5700 2865 554
BtgI C'CryG_G
Cuts at: 107 758 3188 5696 5940 107
Size: 651 2430 2508 244 3286
Fragments arranged by size:
3286 2508 2430 651 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 893 7069 7250 7413 893
Size: 6176 181 163 2599
Fragments arranged by size:
6176 2599 181 163
BtsI GCAGTG_nn'
Cuts at: 223 627 725 983 1677 1861 3314 3726 3785
Size: 404 98 258 694 184 1453 412 59
Cuts at: 3785 4220 6362 223
Size: 435 2142 2980
Fragments arranged by size:
2980 2142 1453 694 435 412 404 258
184 98 59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3035 3070 3035
Size: 35 9084
DraI TTT'AAA
Cuts at: 1220 5765 6118 1220
Size: 4545 353 4221
Fragments arranged by size:
4545 4221 353
DraIII CAC_nnn'GTG
Cuts at: 266 775 2085 2137 266
Size: 509 1310 52 7248
Fragments arranged by size:
7248 1310 509 52
DrdI GACnn_nn'nnGTC
Cuts at: 6825 7683 6825
Size: 858 8261
EaeI y'GGCC_r
Cuts at: 2436 3759 5298 5693 6704 6878 7269 7296 8641
Size: 1323 1539 395 1011 174 391 27 1345
Cuts at: 8641 2436
Size: 2914
Fragments arranged by size:
2914 1539 1345 1323 1011 395 391 174
27
EagI C'GGCC_G
Cuts at: 6704 6704
Size: 9119
EarI CTCTTCn'nnn_
Cuts at: 759 1346 3719 3902 4058 4857 5441 5891 7142
Size: 587 2373 183 156 799 584 450 1251
Cuts at: 7142 7352 759
Size: 210 2526
Fragments arranged by size:
2526 2373 1251 799 587 584 450 210
183 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7327 7637 7783 8929 7327
Size: 310 146 1146 7517
Fragments arranged by size:
7517 1146 310 146
EcoICRI GAG'CTC
Cuts at: 238 6131 238
Size: 5893 3226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 412 628 1349 1750 1861 2008 2044 2213
Size: 219 216 721 401 111 147 36 169
Cuts at: 2213 2362 2545 2771 3223 3353 4093 4213 4573
Size: 149 183 226 452 130 740 120 360
Cuts at: 4573 4847 5969 6944 7376 8123 8391 193
Size: 274 1122 975 432 747 268 921
Fragments arranged by size:
1122 975 921 747 740 721 452 432
401 360 274 268 226 219 216 183
169 149 147 130 120 111 36
EcoNI CCTnn'n_nnAGG
Cuts at: 1367 1367
Size: 9119
EcoO109I rG'GnC_Cy
Cuts at: 1812 3541 3568 6094 6258 1812
Size: 1729 27 2526 164 4673
Fragments arranged by size:
4673 2526 1729 164 27
EcoRI G'AATT_C
Cuts at: 1159 4935 6123 1159
Size: 3776 1188 4155
Fragments arranged by size:
4155 3776 1188
EcoRV GAT'ATC
Cuts at: 4869 4869
Size: 9119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1296 1979 2758 7138 1296
Size: 683 779 4380 3277
Fragments arranged by size:
4380 3277 779 683
FspI TGC'GCA
Cuts at: 2843 6900 2843
Size: 4057 5062
HaeII r_GCGC'y
Cuts at: 6553 6801 7823 8802 6553
Size: 248 1022 979 6870
Fragments arranged by size:
6870 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 177 209 225 257 1482 1514 1981 2013 2301
Size: 32 16 32 1225 32 467 32 288
Cuts at: 2301 2333 2373 2405 2640 2672 2758 2790 3484
Size: 32 40 32 235 32 86 32 694
Cuts at: 3484 3516 5017 5049 5092 5124 5632 5664 6961
Size: 32 1501 32 43 32 508 32 1297
Cuts at: 6961 6991 6993 7023 177
Size: 30 2 30 2273
Fragments arranged by size:
2273 1501 1297 1225 694 508 467 288
235 86 43 40 32 32 32 32
32 32 32 32 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 520 2833 4779 6158 520
Size: 2313 1946 1379 3481
Fragments arranged by size:
3481 2313 1946 1379
HindIII A'AGCT_T
Cuts at: 1961 2783 4540 1961
Size: 822 1757 6540
Fragments arranged by size:
6540 1757 822
KasI G'GCGC_C
Cuts at: 6549 6797 6549
Size: 248 8871
KpnI G_GTAC'C
Cuts at: 6139 6139
Size: 9119
MfeI C'AATT_G
Cuts at: 1315 1315
Size: 9119
MluI A'CGCG_T
Cuts at: 8552 8552
Size: 9119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1958 5728 7790 7974 1958
Size: 3770 2062 184 3103
Fragments arranged by size:
3770 3103 2062 184
MscI TGG'CCA
Cuts at: 2438 3761 5300 6880 2438
Size: 1323 1539 1580 4677
Fragments arranged by size:
4677 1580 1539 1323
MslI CAynn'nnrTG
Cuts at: 770 829 854 1512 2876 2882 3767 3799 4102
Size: 59 25 658 1364 6 885 32 303
Cuts at: 4102 4176 4290 5001 6037 7108 7235 770
Size: 74 114 711 1036 1071 127 2654
Fragments arranged by size:
2654 1364 1071 1036 885 711 658 303
127 114 74 59 32 25 6
MspA1I CmG'CkG
Cuts at: 973 1986 3397 3819 4534 6230 6544 6904 7917
Size: 1013 1411 422 715 1696 314 360 1013
Cuts at: 7917 8162 973
Size: 245 1930
Fragments arranged by size:
1930 1696 1411 1013 1013 715 422 360
314 245
NaeI GCC'GGC
Cuts at: 7300 7300
Size: 9119
NarI GG'CG_CC
Cuts at: 6550 6798 6550
Size: 248 8871
NcoI C'CATG_G
Cuts at: 107 3188 5940 107
Size: 3081 2752 3286
Fragments arranged by size:
3286 3081 2752
NdeI CA'TA_TG
Cuts at: 6072 6072
Size: 9119
NgoMIV G'CCGG_C
Cuts at: 7298 7298
Size: 9119
NheI G'CTAG_C
Cuts at: 1143 1143
Size: 9119
NsiI A_TGCA'T
Cuts at: 4140 4140
Size: 9119
NspI r_CATG'y
Cuts at: 1513 1941 2591 2703 2956 3079 3987 4229 4272
Size: 428 650 112 253 123 908 242 43
Cuts at: 4272 5424 5475 5752 6172 6672 7579 1513
Size: 1152 51 277 420 500 907 3053
Fragments arranged by size:
3053 1152 908 907 650 500 428 420
277 253 242 123 112 51 43
PasI CC'CwG_GG
Cuts at: 4963 4963
Size: 9119
PciI A'CATG_T
Cuts at: 1509 7575 1509
Size: 6066 3053
PflMI CCAn_nnn'nTGG
Cuts at: 1663 5155 5307 5613 5710 5842 1663
Size: 3492 152 306 97 132 4940
Fragments arranged by size:
4940 3492 306 152 132 97
PfoI T'CCnGG_A
Cuts at: 782 782
Size: 9119
PmeI GTTT'AAAC
Cuts at: 6118 6118
Size: 9119
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 398 430 8258 8290 398
Size: 32 7828 32 1227
Fragments arranged by size:
7828 1227 32 32
PpuMI rG'GwC_Cy
Cuts at: 3541 3568 3541
Size: 27 9092
PshAI GACnn'nnGTC
Cuts at: 1629 1629
Size: 9119
PstI C_TGCA'G
Cuts at: 622 978 1116 1587 3324 3780 4533 4923 6166
Size: 356 138 471 1737 456 753 390 1243
Cuts at: 6166 6851 622
Size: 685 2890
Fragments arranged by size:
2890 1737 1243 753 685 471 456 390
356 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9119
PvuII CAG'CTG
Cuts at: 973 1986 3397 4534 6544 6904 973
Size: 1013 1411 1137 2010 360 3188
Fragments arranged by size:
3188 2010 1411 1137 1013 360
RsrII CG'GwC_CG
Cuts at: 7314 7314
Size: 9119
SacI G_AGCT'C
Cuts at: 240 6133 240
Size: 5893 3226
SalI G'TCGA_C
Cuts at: 6156 6156
Size: 9119
SapI GCTCTTCn'nnn_
Cuts at: 7142 7352 7142
Size: 210 8909
SbfI CC_TGCA'GG
Cuts at: 6166 6166
Size: 9119
ScaI AGT'ACT
Cuts at: 8636 8636
Size: 9119
SexAI A'CCwGG_T
Cuts at: 2081 2081
Size: 9119
SfcI C'TryA_G
Cuts at: 33 566 618 740 974 1112 1583 1681 2794
Size: 533 52 122 234 138 471 98 1113
Cuts at: 2794 3320 3776 4529 4699 4919 5403 6162 6847
Size: 526 456 753 170 220 484 759 685
Cuts at: 6847 7840 8031 33
Size: 993 191 1121
Fragments arranged by size:
1121 1113 993 759 753 685 533 526
484 471 456 234 220 191 170 138
122 98 52
SfoI GGC'GCC
Cuts at: 6551 6799 6551
Size: 248 8871
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9119
SgrAI Cr'CCGG_yG
Cuts at: 3890 3890
Size: 9119
SmaI CCC'GGG
Cuts at: 6141 6141
Size: 9119
SmlI C'TyrA_G
Cuts at: 1642 4913 6274 7189 7681 7943 1642
Size: 3271 1361 915 492 262 2818
Fragments arranged by size:
3271 2818 1361 915 492 262
SpeI A'CTAG_T
Cuts at: 67 5601 67
Size: 5534 3585
SphI G_CATG'C
Cuts at: 2591 2956 5752 6172 6672 2591
Size: 365 2796 420 500 5038
Fragments arranged by size:
5038 2796 500 420 365
SspI AAT'ATT
Cuts at: 805 2707 805
Size: 1902 7217
StuI AGG'CCT
Cuts at: 991 1545 991
Size: 554 8565
StyI C'CwwG_G
Cuts at: 107 1954 2037 3188 4024 5149 5544 5940 6253
Size: 1847 83 1151 836 1125 395 396 313
Cuts at: 6253 6607 107
Size: 354 2619
Fragments arranged by size:
2619 1847 1151 1125 836 396 395 354
313 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 920 1984 3690 5142 5346 6702 6835 7294 7503
Size: 1064 1706 1452 204 1356 133 459 209
Cuts at: 7503 920
Size: 2536
Fragments arranged by size:
2536 1706 1452 1356 1064 459 209 204
133
TatI w'GTAC_w
Cuts at: 2532 3986 5250 8634 2532
Size: 1454 1264 3384 3017
Fragments arranged by size:
3384 3017 1454 1264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5681 5713 8636 8668 5681
Size: 32 2923 32 6132
Fragments arranged by size:
6132 2923 32 32
Tth111I GACn'n_nGTC
Cuts at: 149 4770 6033 6916 149
Size: 4621 1263 883 2352
Fragments arranged by size:
4621 2352 1263 883
XbaI T'CTAG_A
Cuts at: 5320 6150 5320
Size: 830 8289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 460 2824 5152 5289 5488 6614 460
Size: 2364 2328 137 199 1126 2965
Fragments arranged by size:
2965 2364 2328 1126 199 137
XmaI C'CCGG_G
Cuts at: 6139 6139
Size: 9119
XmnI GAAnn'nnTTC
Cuts at: 2459 2459
Size: 9119
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII ApaI AscI AvrII BcgI BsiWI
BsmI BssHII BstEII BstZ17I ClaI FseI FspAI HpaI
NotI NruI PacI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SnaBI SrfI SwaI XhoI ZraI