Restriction map for
pF1KB9886
(Circular) (Six-base) MAPSORT of: pF1KB9886.seq Check: 2014 from: 1 to: 5288
pF1KB9886 5288 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1617 1617
Size: 5288
AccI GT'mk_AC
Cuts at: 296 2326 296
Size: 2030 3258
Acc65I G'GTAC_C
Cuts at: 2304 2304
Size: 5288
AclI AA'CG_TT
Cuts at: 5128 5128
Size: 5288
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1151 1841 2023 3113 3545 4292 4560 304
Size: 847 690 182 1090 432 747 268 1032
Fragments arranged by size:
1090 1032 847 747 690 432 268 182
AflIII A'CryG_T
Cuts at: 854 2640 3744 4721 854
Size: 1786 1104 977 1421
Fragments arranged by size:
1786 1421 1104 977
AgeI A'CCGG_T
Cuts at: 3676 3676
Size: 5288
AleI CACnn'nnGTG
Cuts at: 1282 1282
Size: 5288
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 646 646
Size: 5288
AlwNI CAG_nnn'CTG
Cuts at: 258 282 1420 1549 1559 1663 1701 2593 4160
Size: 24 1138 129 10 104 38 892 1567
Cuts at: 4160 258
Size: 1386
Fragments arranged by size:
1567 1386 1138 892 129 104 38 24
10
ApaI G_GGCC'C
Cuts at: 957 957
Size: 5288
ApaLI G'TGCA_C
Cuts at: 715 1377 4058 715
Size: 662 2681 1945
Fragments arranged by size:
2681 1945 662
ApoI r'AATT_y
Cuts at: 1525 2292 4641 4897 1525
Size: 767 2349 256 1916
Fragments arranged by size:
2349 1916 767 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5288
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5288
AvaI C'yCGr_G
Cuts at: 535 877 1001 1510 2308 535
Size: 342 124 509 798 3515
Fragments arranged by size:
3515 798 509 342 124
BamHI G'GATC_C
Cuts at: 1115 2106 2313 1115
Size: 991 207 4090
Fragments arranged by size:
4090 991 207
BanI G'GyrC_C
Cuts at: 541 1456 2304 2718 2966 3001 541
Size: 915 848 414 248 35 2828
Fragments arranged by size:
2828 915 848 414 248 35
BanII G_rGCy'C
Cuts at: 957 2302 3332 957
Size: 1345 1030 2913
Fragments arranged by size:
2913 1345 1030
BbeI G_GCGC'C
Cuts at: 2722 2970 2722
Size: 248 5040
BbsI GAAGACnn'nnnn_
Cuts at: 1139 1485 2083 1139
Size: 346 598 4344
Fragments arranged by size:
4344 598 346
BbvCI CC'TCA_GC
Cuts at: 764 764
Size: 5288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 126 160 126
Size: 34 5254
BciVI GTATCCnnnnn_n'
Cuts at: 3186 3947 5288 3186
Size: 761 1341 3186
Fragments arranged by size:
3186 1341 761
BclI T'GATC_A
Cuts at: 2808 2808
Size: 5288
BfrBI ATG'CAT
Cuts at: 2140 2236 2140
Size: 96 5192
BglI GCCn_nnn'nGGC
Cuts at: 518 768 518
Size: 250 5038
BglII A'GATC_T
Cuts at: 335 2223 2803 4840 335
Size: 1888 580 2037 783
Fragments arranged by size:
2037 1888 783 580
BlpI GC'TnA_GC
Cuts at: 367 2400 367
Size: 2033 3255
Bme1580I G_kGCm'C
Cuts at: 719 957 1381 2913 3006 4062 719
Size: 238 424 1532 93 1056 1945
Fragments arranged by size:
1945 1532 1056 424 238 93
BmrI ACTGGGnnnn_n'
Cuts at: 1711 2500 2581 2916 5060 1711
Size: 789 81 335 2144 1939
Fragments arranged by size:
2144 1939 789 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 520 1668 3655 520
Size: 1148 1987 2153
Fragments arranged by size:
2153 1987 1148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 214 367 421 482 655 958 178
Size: 36 153 54 61 173 303 4508
Fragments arranged by size:
4508 303 173 153 61 54 36
Bpu10I CC'TnA_GC
Cuts at: 764 1682 764
Size: 918 4370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 2464 3343 3835 4133 625
Size: 1839 879 492 298 1780
Fragments arranged by size:
1839 1780 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 491 4992 491
Size: 4501 787
BsaAI yAC'GTr
Cuts at: 189 2641 189
Size: 2452 2836
BsaBI GATnn'nnATC
Cuts at: 6 2802 4839 6
Size: 2796 2037 455
Fragments arranged by size:
2796 2037 455
BsaHI Gr'CG_yC
Cuts at: 640 1852 2719 2967 3669 5165 640
Size: 1212 867 248 702 1496 763
Fragments arranged by size:
1496 1212 867 763 702 248
BsaWI w'CCGG_w
Cuts at: 559 2477 2998 3676 3950 4097 4508 559
Size: 1918 521 678 274 147 411 1339
Fragments arranged by size:
1918 1339 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2167 2197 2167
Size: 30 5258
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 484 1000 1273 1848 280
Size: 204 516 273 575 3720
Fragments arranged by size:
3720 575 516 273 204
BseYI C'CCAG_C
Cuts at: 1368 1934 2713 3471 4048 4544 1368
Size: 566 779 758 577 496 2112
Fragments arranged by size:
2112 779 758 577 566 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2008 2008
Size: 5288
BsiEI CG_ry'CG
Cuts at: 104 2830 2876 4084 104
Size: 2726 46 1208 1308
Fragments arranged by size:
2726 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 239 719 1381 2302 3080 3270 4062 239
Size: 480 662 921 778 190 792 1465
Fragments arranged by size:
1465 921 792 778 662 480 190
BsmI GAATG_Cn'
Cuts at: 2142 2238 2142
Size: 96 5192
BsmBI CGTCTCn'nnnn_
Cuts at: 262 316 631 262
Size: 54 315 4919
Fragments arranged by size:
4919 315 54
Bsp1286I G_dGCh'C
Cuts at: 239 719 957 1381 2302 2913 3006 3080 3270
Size: 480 238 424 921 611 93 74 190
Cuts at: 3270 3332 4062 239
Size: 62 730 1465
Fragments arranged by size:
1465 921 730 611 480 424 238 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4508 4508
Size: 5288
BspHI T'CATG_A
Cuts at: 728 4464 728
Size: 3736 1552
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1190 1617 2214 2338 2552 2854 3235 1190
Size: 427 597 124 214 302 381 3243
Fragments arranged by size:
3243 597 427 381 302 214 124
BsrBI CCG'CTC
Cuts at: 50 3580 3634 5118 5283 50
Size: 3530 54 1484 165 55
Fragments arranged by size:
3530 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1148 1689 3200 1148
Size: 541 1511 3236
Fragments arranged by size:
3236 1511 541
BsrFI r'CCGG_y
Cuts at: 239 308 517 3286 3467 3676 239
Size: 69 209 2769 181 209 1851
Fragments arranged by size:
2769 1851 209 209 181 69
BsrGI T'GTAC_A
Cuts at: 659 659
Size: 5288
BssSI C'ACGA_G
Cuts at: 3559 3917 3559
Size: 358 4930
BstAPI GCAn_nnn'nTGC
Cuts at: 714 2665 714
Size: 1951 3337
BstBI TT'CG_AA
Cuts at: 1295 3649 1295
Size: 2354 2934
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5288
BstYI r'GATC_y
Cuts at: 1 335 1115 1719 2106 2223 2313 2780 2803
Size: 334 780 604 387 117 90 467 23
Cuts at: 2803 3138 3384 4396 4482 4494 4840 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 780 604 467 449 387 346 335
334 246 117 90 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1076 1076
Size: 5288
BtgI C'CryG_G
Cuts at: 107 1106 1595 1979 107
Size: 999 489 384 3416
Fragments arranged by size:
3416 999 489 384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 127 133 1795 3238 3419 3582 127
Size: 6 1662 1443 181 163 1833
Fragments arranged by size:
1833 1662 1443 181 163 6
BtsI GCAGTG_nn'
Cuts at: 2531 2531
Size: 5288
ClaI AT'CG_AT
Cuts at: 1535 1535
Size: 5288
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 585 620 2258 2293 585
Size: 35 1638 35 3580
Fragments arranged by size:
3580 1638 35 35
DraI TTT'AAA
Cuts at: 2287 2287
Size: 5288
DrdI GACnn_nn'nnGTC
Cuts at: 1141 2994 3852 1141
Size: 1853 858 2577
Fragments arranged by size:
2577 1853 858
EaeI y'GGCC_r
Cuts at: 815 2873 3047 3438 3465 4810 815
Size: 2058 174 391 27 1345 1293
Fragments arranged by size:
2058 1345 1293 391 174 27
EagI C'GGCC_G
Cuts at: 2873 2873
Size: 5288
EarI CTCTTCn'nnn_
Cuts at: 1082 1817 1874 1988 3311 3521 1082
Size: 735 57 114 1323 210 2849
Fragments arranged by size:
2849 1323 735 210 114 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 277 3496 3806 3952 5098 277
Size: 3219 310 146 1146 467
Fragments arranged by size:
3219 1146 467 310 146
EcoICRI GAG'CTC
Cuts at: 2300 2300
Size: 5288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 214 304 367 421 482 655 958 1151
Size: 36 90 63 54 61 173 303 193
Cuts at: 1151 1841 2023 3113 3545 4292 4560 178
Size: 690 182 1090 432 747 268 906
Fragments arranged by size:
1090 906 747 690 432 303 268 193
182 173 90 63 61 54 36
EcoO109I rG'GnC_Cy
Cuts at: 225 669 953 954 1766 1998 2427 225
Size: 444 284 1 812 232 429 3086
Fragments arranged by size:
3086 812 444 429 284 232 1
EcoRI G'AATT_C
Cuts at: 2292 2292
Size: 5288
EcoRV GAT'ATC
Cuts at: 1710 1710
Size: 5288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3307 3307
Size: 5288
FspI TGC'GCA
Cuts at: 1154 3069 1154
Size: 1915 3373
HaeII r_GCGC'y
Cuts at: 259 2722 2970 3992 4971 259
Size: 2463 248 1022 979 576
Fragments arranged by size:
2463 1022 979 576 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1311 1343 1706 1738 3130 3160 3162 3192 1311
Size: 32 363 32 1392 30 2 30 3407
Fragments arranged by size:
3407 1392 363 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1179 2327 1179
Size: 1148 4140
HindIII A'AGCT_T
Cuts at: 2120 2120
Size: 5288
KasI G'GCGC_C
Cuts at: 2718 2966 2718
Size: 248 5040
KpnI G_GTAC'C
Cuts at: 2308 2308
Size: 5288
MluI A'CGCG_T
Cuts at: 4721 4721
Size: 5288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 104 191 3959 4143 104
Size: 87 3768 184 1249
Fragments arranged by size:
3768 1249 184 87
MscI TGG'CCA
Cuts at: 3049 3049
Size: 5288
MslI CAynn'nnrTG
Cuts at: 112 1282 1600 1606 3277 3404 112
Size: 1170 318 6 1671 127 1996
Fragments arranged by size:
1996 1671 1170 318 127 6
MspA1I CmG'CkG
Cuts at: 156 433 711 768 994 1069 1420 1556 1663
Size: 277 278 57 226 75 351 136 107
Cuts at: 1663 2399 2713 3073 4086 4331 156
Size: 736 314 360 1013 245 1113
Fragments arranged by size:
1113 1013 736 360 351 314 278 277
245 226 136 107 75 57
NaeI GCC'GGC
Cuts at: 519 3469 519
Size: 2950 2338
NarI GG'CG_CC
Cuts at: 2719 2967 2719
Size: 248 5040
NcoI C'CATG_G
Cuts at: 107 1106 1595 1979 107
Size: 999 489 384 3416
Fragments arranged by size:
3416 999 489 384
NgoMIV G'CCGG_C
Cuts at: 517 3467 517
Size: 2950 2338
NsiI A_TGCA'T
Cuts at: 2142 2238 2142
Size: 96 5192
NspI r_CATG'y
Cuts at: 858 1605 2080 2341 2841 3748 858
Size: 747 475 261 500 907 2398
Fragments arranged by size:
2398 907 747 500 475 261
PasI CC'CwG_GG
Cuts at: 1863 1863
Size: 5288
PciI A'CATG_T
Cuts at: 854 3744 854
Size: 2890 2398
PflMI CCAn_nnn'nTGG
Cuts at: 258 1112 1206 1701 258
Size: 854 94 495 3845
Fragments arranged by size:
3845 854 495 94
PfoI T'CCnGG_A
Cuts at: 230 1118 230
Size: 888 4400
PmeI GTTT'AAAC
Cuts at: 2287 2287
Size: 5288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 4427
Size: 32 5256
PpuMI rG'GwC_Cy
Cuts at: 225 669 225
Size: 444 4844
PspOMI G'GGCC_C
Cuts at: 953 953
Size: 5288
PspXI vC'TCGA_Gb
Cuts at: 1001 1001
Size: 5288
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1933 1965 1933
Size: 32 5256
PstI C_TGCA'G
Cuts at: 625 710 2335 3020 625
Size: 85 1625 685 2893
Fragments arranged by size:
2893 1625 685 85
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5288
PvuII CAG'CTG
Cuts at: 156 1069 1663 2713 3073 156
Size: 913 594 1050 360 2371
Fragments arranged by size:
2371 1050 913 594 360
RsrII CG'GwC_CG
Cuts at: 3483 3483
Size: 5288
SacI G_AGCT'C
Cuts at: 2302 2302
Size: 5288
SalI G'TCGA_C
Cuts at: 2325 2325
Size: 5288
SapI GCTCTTCn'nnn_
Cuts at: 3311 3521 3311
Size: 210 5078
SbfI CC_TGCA'GG
Cuts at: 2335 2335
Size: 5288
ScaI AGT'ACT
Cuts at: 652 4805 652
Size: 4153 1135
SfcI C'TryA_G
Cuts at: 33 621 706 1655 2331 3016 4009 4200 33
Size: 588 85 949 676 685 993 191 1121
Fragments arranged by size:
1121 993 949 685 676 588 191 85
SfoI GGC'GCC
Cuts at: 2720 2968 2720
Size: 248 5040
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5288
SmaI CCC'GGG
Cuts at: 537 2310 537
Size: 1773 3515
SmlI C'TyrA_G
Cuts at: 604 1001 1510 2443 3358 3850 4112 604
Size: 397 509 933 915 492 262 1780
Fragments arranged by size:
1780 933 915 509 492 397 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5288
SphI G_CATG'C
Cuts at: 2341 2841 2341
Size: 500 4788
StuI AGG'CCT
Cuts at: 1387 1387
Size: 5288
StyI C'CwwG_G
Cuts at: 107 1025 1106 1595 1761 1979 2422 2776 107
Size: 918 81 489 166 218 443 354 2619
Fragments arranged by size:
2619 918 489 443 354 218 166 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 397 1693 1926 2871 3004 3463 3672 397
Size: 1296 233 945 133 459 209 2013
Fragments arranged by size:
2013 1296 945 459 233 209 133
TatI w'GTAC_w
Cuts at: 650 659 1916 2266 4803 650
Size: 9 1257 350 2537 1135
Fragments arranged by size:
2537 1257 1135 350 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4805 4837 4805
Size: 32 5256
Tth111I GACn'n_nGTC
Cuts at: 3085 3085
Size: 5288
XbaI T'CTAG_A
Cuts at: 2239 2319 2239
Size: 80 5208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1977 1986 2783 1977
Size: 9 797 4482
Fragments arranged by size:
4482 797 9
XhoI C'TCGA_G
Cuts at: 1001 1510 1001
Size: 509 4779
XmaI C'CCGG_G
Cuts at: 535 2308 535
Size: 1773 3515
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BmgBI BmtI BsiWI BssHII BstEII BstZ17I DraIII EcoNI
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PmlI PshAI PsiI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI ZraI