Restriction map for
pF1KB9883
(Circular) (Six-base) MAPSORT of: pF1KB9883.seq Check: 2843 from: 1 to: 4982
pF1KB9883 4982 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 2020 2020
Size: 4982
Acc65I G'GTAC_C
Cuts at: 1998 1998
Size: 4982
AclI AA'CG_TT
Cuts at: 390 4822 390
Size: 4432 550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 856 988 2807 3239 3986 4254 102
Size: 754 132 1819 432 747 268 830
Fragments arranged by size:
1819 830 754 747 432 268 132
AfeI AGC'GCT
Cuts at: 973 973
Size: 4982
AflIII A'CryG_T
Cuts at: 2334 3438 4415 2334
Size: 1104 977 2901
Fragments arranged by size:
2901 1104 977
AgeI A'CCGG_T
Cuts at: 3370 3370
Size: 4982
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 826 1355 826
Size: 529 4453
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1457 1489 1457
Size: 32 4950
AlwNI CAG_nnn'CTG
Cuts at: 198 834 1503 2287 3854 198
Size: 636 669 784 1567 1326
Fragments arranged by size:
1567 1326 784 669 636
ApaLI G'TGCA_C
Cuts at: 1669 3752 1669
Size: 2083 2899
ApoI r'AATT_y
Cuts at: 1619 1986 4335 4591 1619
Size: 367 2349 256 2010
Fragments arranged by size:
2349 2010 367 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4982
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
AvaI C'yCGr_G
Cuts at: 239 1128 2002 239
Size: 889 874 3219
Fragments arranged by size:
3219 889 874
BamHI G'GATC_C
Cuts at: 2007 2007
Size: 4982
BanI G'GyrC_C
Cuts at: 419 1238 1331 1998 2412 2660 2695 419
Size: 819 93 667 414 248 35 2706
Fragments arranged by size:
2706 819 667 414 248 93 35
BanII G_rGCy'C
Cuts at: 820 1081 1174 1384 1445 1712 1996 3026 820
Size: 261 93 210 61 267 284 1030 2776
Fragments arranged by size:
2776 1030 284 267 261 210 93 61
BbeI G_GCGC'C
Cuts at: 423 1242 2416 2664 423
Size: 819 1174 248 2741
Fragments arranged by size:
2741 1174 819 248
BbsI GAAGACnn'nnnn_
Cuts at: 677 677
Size: 4982
BbvCI CC'TCA_GC
Cuts at: 1436 1528 1436
Size: 92 4890
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1208 1242 1208
Size: 34 4948
BciVI GTATCCnnnnn_n'
Cuts at: 917 2880 3641 4982 917
Size: 1963 761 1341 917
Fragments arranged by size:
1963 1341 917 761
BclI T'GATC_A
Cuts at: 1923 2502 1923
Size: 579 4403
BglI GCCn_nnn'nGGC
Cuts at: 321 321
Size: 4982
BglII A'GATC_T
Cuts at: 2497 4534 2497
Size: 2037 2945
BlpI GC'TnA_GC
Cuts at: 2094 2094
Size: 4982
Bme1580I G_kGCm'C
Cuts at: 695 1127 1673 2607 2700 3756 695
Size: 432 546 934 93 1056 1921
Fragments arranged by size:
1921 1056 934 546 432 93
BmrI ACTGGGnnnn_n'
Cuts at: 2194 2275 2610 4754 2194
Size: 81 335 2144 2422
Fragments arranged by size:
2422 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1922 3349 1922
Size: 1427 3555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 783 1021 1057 1210 1869 783
Size: 238 36 153 659 3896
Fragments arranged by size:
3896 659 238 153 36
Bpu10I CC'TnA_GC
Cuts at: 361 950 1082 1378 1436 1528 361
Size: 589 132 296 58 92 3815
Fragments arranged by size:
3815 589 296 132 92 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 2158 3037 3529 3827 892
Size: 1266 879 492 298 2047
Fragments arranged by size:
2047 1266 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 608 4686 608
Size: 4078 904
BsaAI yAC'GTr
Cuts at: 1668 2335 1668
Size: 667 4315
BsaBI GATnn'nnATC
Cuts at: 6 2496 4533 6
Size: 2490 2037 455
Fragments arranged by size:
2490 2037 455
BsaHI Gr'CG_yC
Cuts at: 420 1239 2413 2661 3363 4859 420
Size: 819 1174 248 702 1496 543
Fragments arranged by size:
1496 1174 819 702 543 248
BsaWI w'CCGG_w
Cuts at: 214 2171 2692 3370 3644 3791 4202 214
Size: 1957 521 678 274 147 411 994
Fragments arranged by size:
1957 994 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1256 1286 1366 1396 1756 1786 1256
Size: 30 80 30 360 30 4452
Fragments arranged by size:
4452 360 80 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 575 976 1317 1453 1909 575
Size: 401 341 136 456 3648
Fragments arranged by size:
3648 456 401 341 136
BseYI C'CCAG_C
Cuts at: 131 198 271 482 494 785 1943 2407 3165
Size: 67 73 211 12 291 1158 464 758
Cuts at: 3165 3742 4238 131
Size: 577 496 875
Fragments arranged by size:
1158 875 758 577 496 464 291 211
73 67 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 1714 1843 271
Size: 1443 129 3410
Fragments arranged by size:
3410 1443 129
BsiEI CG_ry'CG
Cuts at: 104 502 2524 2570 3778 104
Size: 398 2022 46 1208 1308
Fragments arranged by size:
2022 1308 1208 398 46
BsiHKAI G_wGCw'C
Cuts at: 238 334 1217 1384 1445 1673 1712 1996 2774
Size: 96 883 167 61 228 39 284 778
Cuts at: 2774 2964 3756 238
Size: 190 792 1464
Fragments arranged by size:
1464 883 792 778 284 228 190 167
96 61 39
BsmBI CGTCTCn'nnnn_
Cuts at: 910 910
Size: 4982
Bsp1286I G_dGCh'C
Cuts at: 238 334 695 820 1081 1127 1174 1217 1384
Size: 96 361 125 261 46 47 43 167
Cuts at: 1384 1445 1673 1712 1996 2607 2700 2774 2964
Size: 61 228 39 284 611 93 74 190
Cuts at: 2964 3026 3756 238
Size: 62 730 1464
Fragments arranged by size:
1464 730 611 361 284 261 228 190
167 125 96 93 74 62 61 47
46 43 39
BspEI T'CCGG_A
Cuts at: 214 4202 214
Size: 3988 994
BspHI T'CATG_A
Cuts at: 4158 4158
Size: 4982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2032 2246 2548 2929 2032
Size: 214 302 381 4085
Fragments arranged by size:
4085 381 302 214
BsrBI CCG'CTC
Cuts at: 50 681 1005 1515 3274 3328 4812 4977 50
Size: 631 324 510 1759 54 1484 165 55
Fragments arranged by size:
1759 1484 631 510 324 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2894 2894
Size: 4982
BsrFI r'CCGG_y
Cuts at: 404 1013 1139 2980 3161 3370 404
Size: 609 126 1841 181 209 2016
Fragments arranged by size:
2016 1841 609 209 181 126
BssHII G'CGCG_C
Cuts at: 1198 1200 1578 1198
Size: 2 378 4602
Fragments arranged by size:
4602 378 2
BssSI C'ACGA_G
Cuts at: 3253 3611 3253
Size: 358 4624
BstAPI GCAn_nnn'nTGC
Cuts at: 1290 1821 2359 1290
Size: 531 538 3913
Fragments arranged by size:
3913 538 531
BstBI TT'CG_AA
Cuts at: 3343 3343
Size: 4982
BstYI r'GATC_y
Cuts at: 1 147 1646 2007 2474 2497 2832 3078 4090
Size: 146 1499 361 467 23 335 246 1012
Cuts at: 4090 4176 4188 4534 1
Size: 86 12 346 449
Fragments arranged by size:
1499 1012 467 449 361 346 335 246
146 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1447 1504 1720 1877 1447
Size: 57 216 157 4552
Fragments arranged by size:
4552 216 157 57
BtgI C'CryG_G
Cuts at: 107 163 310 1343 107
Size: 56 147 1033 3746
Fragments arranged by size:
3746 1033 147 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 380 2932 3113 3276 380
Size: 2552 181 163 2086
Fragments arranged by size:
2552 2086 181 163
BtsI GCAGTG_nn'
Cuts at: 340 632 1048 2225 340
Size: 292 416 1177 3097
Fragments arranged by size:
3097 1177 416 292
DraI TTT'AAA
Cuts at: 1981 1981
Size: 4982
DraIII CAC_nnn'GTG
Cuts at: 233 610 233
Size: 377 4605
DrdI GACnn_nn'nnGTC
Cuts at: 647 2688 3546 647
Size: 2041 858 2083
Fragments arranged by size:
2083 2041 858
EaeI y'GGCC_r
Cuts at: 499 2567 2741 3132 3159 4504 499
Size: 2068 174 391 27 1345 977
Fragments arranged by size:
2068 1345 977 391 174 27
EagI C'GGCC_G
Cuts at: 499 2567 499
Size: 2068 2914
EarI CTCTTCn'nnn_
Cuts at: 733 832 845 1547 3005 3215 733
Size: 99 13 702 1458 210 2500
Fragments arranged by size:
2500 1458 702 210 99 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 188 1651 3190 3500 3646 4792 188
Size: 1463 1539 310 146 1146 378
Fragments arranged by size:
1539 1463 1146 378 310 146
EcoICRI GAG'CTC
Cuts at: 1382 1443 1710 1994 1382
Size: 61 267 284 4370
Fragments arranged by size:
4370 284 267 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 783 856 988 1021 1057 1210 1869 2807
Size: 681 73 132 33 36 153 659 938
Cuts at: 2807 3239 3986 4254 102
Size: 432 747 268 830
Fragments arranged by size:
938 830 747 681 659 432 268 153
132 73 36 33
EcoNI CCTnn'n_nnAGG
Cuts at: 1507 1507
Size: 4982
EcoO109I rG'GnC_Cy
Cuts at: 380 831 1410 1500 2121 380
Size: 451 579 90 621 3241
Fragments arranged by size:
3241 621 579 451 90
EcoRI G'AATT_C
Cuts at: 1619 1986 1619
Size: 367 4615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3001 3001
Size: 4982
FspI TGC'GCA
Cuts at: 1612 2763 1612
Size: 1151 3831
HaeII r_GCGC'y
Cuts at: 423 975 1014 1242 1906 2416 2664 3686 4665
Size: 552 39 228 664 510 248 1022 979
Cuts at: 4665 423
Size: 740
Fragments arranged by size:
1022 979 740 664 552 510 248 228
39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2824 2854 2856 2886 2824
Size: 30 2 30 4920
Fragments arranged by size:
4920 30 30 2
HincII GTy'rAC
Cuts at: 2021 2021
Size: 4982
KasI G'GCGC_C
Cuts at: 419 1238 2412 2660 419
Size: 819 1174 248 2741
Fragments arranged by size:
2741 1174 819 248
KpnI G_GTAC'C
Cuts at: 2002 2002
Size: 4982
MluI A'CGCG_T
Cuts at: 4415 4415
Size: 4982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1674 3653 3837 1674
Size: 1979 184 2819
Fragments arranged by size:
2819 1979 184
MscI TGG'CCA
Cuts at: 2743 2743
Size: 4982
MslI CAynn'nnrTG
Cuts at: 1337 2971 3098 1337
Size: 1634 127 3221
Fragments arranged by size:
3221 1634 127
MspA1I CmG'CkG
Cuts at: 165 195 312 486 498 1296 1455 1479 2093
Size: 30 117 174 12 798 159 24 614
Cuts at: 2093 2407 2767 3780 4025 165
Size: 314 360 1013 245 1122
Fragments arranged by size:
1122 1013 798 614 360 314 245 174
159 117 30 24 12
NaeI GCC'GGC
Cuts at: 1015 3163 1015
Size: 2148 2834
NarI GG'CG_CC
Cuts at: 420 1239 2413 2661 420
Size: 819 1174 248 2741
Fragments arranged by size:
2741 1174 819 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4982
NgoMIV G'CCGG_C
Cuts at: 1013 3161 1013
Size: 2148 2834
NotI GC'GGCC_GC
Cuts at: 499 499
Size: 4982
NspI r_CATG'y
Cuts at: 1342 2035 2535 3442 1342
Size: 693 500 907 2882
Fragments arranged by size:
2882 907 693 500
PasI CC'CwG_GG
Cuts at: 531 531
Size: 4982
PciI A'CATG_T
Cuts at: 3438 3438
Size: 4982
PflMI CCAn_nnn'nTGG
Cuts at: 792 792
Size: 4982
PfoI T'CCnGG_A
Cuts at: 394 1274 1364 1703 1856 1885 394
Size: 880 90 339 153 29 3491
Fragments arranged by size:
3491 880 339 153 90 29
PmeI GTTT'AAAC
Cuts at: 1981 1981
Size: 4982
PmlI CAC'GTG
Cuts at: 1668 1668
Size: 4982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 4121
Size: 32 4950
PpuMI rG'GwC_Cy
Cuts at: 831 1410 831
Size: 579 4403
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1957 1989 1957
Size: 32 4950
PstI C_TGCA'G
Cuts at: 938 1295 1301 2029 2714 938
Size: 357 6 728 685 3206
Fragments arranged by size:
3206 728 685 357 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4982
PvuII CAG'CTG
Cuts at: 486 1296 1455 2407 2767 486
Size: 810 159 952 360 2701
Fragments arranged by size:
2701 952 810 360 159
RsrII CG'GwC_CG
Cuts at: 3177 3177
Size: 4982
SacI G_AGCT'C
Cuts at: 1384 1445 1712 1996 1384
Size: 61 267 284 4370
Fragments arranged by size:
4370 284 267 61
SacII CC_GC'GG
Cuts at: 166 313 166
Size: 147 4835
SalI G'TCGA_C
Cuts at: 2019 2019
Size: 4982
SapI GCTCTTCn'nnn_
Cuts at: 832 1547 3005 3215 832
Size: 715 1458 210 2599
Fragments arranged by size:
2599 1458 715 210
SbfI CC_TGCA'GG
Cuts at: 2029 2029
Size: 4982
ScaI AGT'ACT
Cuts at: 4499 4499
Size: 4982
SexAI A'CCwGG_T
Cuts at: 755 755
Size: 4982
SfcI C'TryA_G
Cuts at: 33 934 1291 1297 2025 2710 3703 3894 33
Size: 901 357 6 728 685 993 191 1121
Fragments arranged by size:
1121 993 901 728 685 357 191 6
SfoI GGC'GCC
Cuts at: 421 1240 2414 2662 421
Size: 819 1174 248 2741
Fragments arranged by size:
2741 1174 819 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
SmaI CCC'GGG
Cuts at: 241 1130 2004 241
Size: 889 874 3219
Fragments arranged by size:
3219 889 874
SmlI C'TyrA_G
Cuts at: 907 2137 3052 3544 3806 907
Size: 1230 915 492 262 2083
Fragments arranged by size:
2083 1230 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4982
SphI G_CATG'C
Cuts at: 2035 2535 2035
Size: 500 4482
StuI AGG'CCT
Cuts at: 955 1882 955
Size: 927 4055
StyI C'CwwG_G
Cuts at: 107 791 1430 1836 2116 2470 107
Size: 684 639 406 280 354 2619
Fragments arranged by size:
2619 684 639 406 354 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 841 2565 2698 3157 3366 841
Size: 1724 133 459 209 2457
Fragments arranged by size:
2457 1724 459 209 133
TatI w'GTAC_w
Cuts at: 4497 4497
Size: 4982
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 240 272 1126 1158 1756 1788 4499 4531 240
Size: 32 854 32 598 32 2711 32 691
Fragments arranged by size:
2711 854 691 598 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2779 2779
Size: 4982
XbaI T'CTAG_A
Cuts at: 2013 2013
Size: 4982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 195 2477 195
Size: 2282 2700
XmaI C'CCGG_G
Cuts at: 239 1128 2002 239
Size: 889 874 3219
Fragments arranged by size:
3219 889 874
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BstEII
BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PshAI
PsiI PspOMI PspXI SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI