Restriction map for
pF1KB9878
(Circular) (Six-base) MAPSORT of: pF1KB9878.seq Check: 467 from: 1 to: 4838
pF1KB9878 4838 bp
With 182 enzymes: SgfI *
January 12, 2011 16:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 296 1780 296
Size: 1484 3354
AatII G_ACGT'C
Cuts at: 916 916
Size: 4838
AccI GT'mk_AC
Cuts at: 1355 1876 1355
Size: 521 4317
Acc65I G'GTAC_C
Cuts at: 1138 1854 1138
Size: 716 4122
AclI AA'CG_TT
Cuts at: 4678 4678
Size: 4838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 1300 1367 1622 2663 3095 3842 4110 484
Size: 816 67 255 1041 432 747 268 1212
Fragments arranged by size:
1212 1041 816 747 432 268 255 67
AfeI AGC'GCT
Cuts at: 1728 1728
Size: 4838
AflIII A'CryG_T
Cuts at: 2190 3294 4271 2190
Size: 1104 977 2757
Fragments arranged by size:
2757 1104 977
AgeI A'CCGG_T
Cuts at: 3226 3226
Size: 4838
AleI CACnn'nnGTG
Cuts at: 211 211
Size: 4838
AlwNI CAG_nnn'CTG
Cuts at: 2143 3710 2143
Size: 1567 3271
ApaI G_GGCC'C
Cuts at: 324 324
Size: 4838
ApaLI G'TGCA_C
Cuts at: 3608 3608
Size: 4838
ApoI r'AATT_y
Cuts at: 1842 4191 4447 1842
Size: 2349 256 2233
Fragments arranged by size:
2349 2233 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4838
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
AvaI C'yCGr_G
Cuts at: 277 484 787 806 1267 1858 277
Size: 207 303 19 461 591 3257
Fragments arranged by size:
3257 591 461 303 207 19
BamHI G'GATC_C
Cuts at: 1863 1863
Size: 4838
BanI G'GyrC_C
Cuts at: 199 273 1138 1854 2268 2516 2551 199
Size: 74 865 716 414 248 35 2486
Fragments arranged by size:
2486 865 716 414 248 74 35
BanII G_rGCy'C
Cuts at: 236 324 796 1652 1852 2882 236
Size: 88 472 856 200 1030 2192
Fragments arranged by size:
2192 1030 856 472 200 88
BbeI G_GCGC'C
Cuts at: 2272 2520 2272
Size: 248 4590
BbsI GAAGACnn'nnnn_
Cuts at: 472 1212 472
Size: 740 4098
BbvCI CC'TCA_GC
Cuts at: 121 150 176 121
Size: 29 26 4783
Fragments arranged by size:
4783 29 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 801 835 801
Size: 34 4804
BciVI GTATCCnnnnn_n'
Cuts at: 2736 3497 4838 2736
Size: 761 1341 2736
Fragments arranged by size:
2736 1341 761
BclI T'GATC_A
Cuts at: 2358 2358
Size: 4838
BglII A'GATC_T
Cuts at: 2353 4390 2353
Size: 2037 2801
BlpI GC'TnA_GC
Cuts at: 1950 1950
Size: 4838
Bme1580I G_kGCm'C
Cuts at: 204 278 324 635 2463 2556 3612 204
Size: 74 46 311 1828 93 1056 1430
Fragments arranged by size:
1828 1430 1056 311 93 74 46
BmgBI CAC'GTC
Cuts at: 950 950
Size: 4838
BmrI ACTGGGnnnn_n'
Cuts at: 1456 1593 2050 2131 2466 4610 1456
Size: 137 457 81 335 2144 1684
Fragments arranged by size:
2144 1684 457 335 137 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3205
Size: 4838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 886 957 1329 313
Size: 573 71 372 3822
Fragments arranged by size:
3822 573 372 71
Bpu10I CC'TnA_GC
Cuts at: 121 150 176 121
Size: 29 26 4783
Fragments arranged by size:
4783 29 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 2014 2893 3385 3683 314
Size: 1700 879 492 298 1469
Fragments arranged by size:
1700 1469 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4542 4542
Size: 4838
BsaAI yAC'GTr
Cuts at: 1611 2191 1611
Size: 580 4258
BsaBI GATnn'nnATC
Cuts at: 6 2352 4389 6
Size: 2346 2037 455
Fragments arranged by size:
2346 2037 455
BsaHI Gr'CG_yC
Cuts at: 578 913 1285 2269 2517 3219 4715 578
Size: 335 372 984 248 702 1496 701
Fragments arranged by size:
1496 984 702 701 372 335 248
BsaWI w'CCGG_w
Cuts at: 2027 2548 3226 3500 3647 4058 2027
Size: 521 678 274 147 411 2807
Fragments arranged by size:
2807 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 631 661 994 1024 631
Size: 30 333 30 4445
Fragments arranged by size:
4445 333 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 164 667 694 739 742 1623 1660 164
Size: 503 27 45 3 881 37 3342
Fragments arranged by size:
3342 881 503 45 37 27 3
BseYI C'CCAG_C
Cuts at: 183 495 568 672 1299 2263 3021 3598 4094
Size: 312 73 104 627 964 758 577 496
Cuts at: 4094 183
Size: 927
Fragments arranged by size:
964 927 758 627 577 496 312 104
73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1758 1758
Size: 4838
BsiEI CG_ry'CG
Cuts at: 104 360 392 558 622 2380 2426 3634 104
Size: 256 32 166 64 1758 46 1208 1308
Fragments arranged by size:
1758 1308 1208 256 166 64 46 32
BsiHKAI G_wGCw'C
Cuts at: 796 1652 1852 2630 2820 3612 796
Size: 856 200 778 190 792 2022
Fragments arranged by size:
2022 856 792 778 200 190
BsmBI CGTCTCn'nnnn_
Cuts at: 448 481 862 997 448
Size: 33 381 135 4289
Fragments arranged by size:
4289 381 135 33
Bsp1286I G_dGCh'C
Cuts at: 204 236 278 324 635 796 1652 1852 2463
Size: 32 42 46 311 161 856 200 611
Cuts at: 2463 2556 2630 2820 2882 3612 204
Size: 93 74 190 62 730 1430
Fragments arranged by size:
1430 856 730 611 311 200 190 161
93 74 62 46 42 32
BspEI T'CCGG_A
Cuts at: 4058 4058
Size: 4838
BspHI T'CATG_A
Cuts at: 1088 4014 1088
Size: 2926 1912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 296 1780 1888 2102 2404 2785 296
Size: 1484 108 214 302 381 2349
Fragments arranged by size:
2349 1484 381 302 214 108
BsrBI CCG'CTC
Cuts at: 50 534 732 3130 3184 4668 4833 50
Size: 484 198 2398 54 1484 165 55
Fragments arranged by size:
2398 1484 484 198 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1170 1525 2750 1170
Size: 355 1225 3258
Fragments arranged by size:
3258 1225 355
BsrFI r'CCGG_y
Cuts at: 387 563 617 621 801 937 1507 2836 3017
Size: 176 54 4 180 136 570 1329 181
Cuts at: 3017 3226 387
Size: 209 1999
Fragments arranged by size:
1999 1329 570 209 181 180 176 136
54 4
BssSI C'ACGA_G
Cuts at: 3109 3467 3109
Size: 358 4480
BstAPI GCAn_nnn'nTGC
Cuts at: 2215 2215
Size: 4838
BstBI TT'CG_AA
Cuts at: 929 1031 3199 929
Size: 102 2168 2568
Fragments arranged by size:
2568 2168 102
BstEII G'GTnAC_C
Cuts at: 1425 1425
Size: 4838
BstXI CCAn_nnnn'nTGG
Cuts at: 1185 1185
Size: 4838
BstYI r'GATC_y
Cuts at: 1 656 1262 1489 1863 2330 2353 2688 2934
Size: 655 606 227 374 467 23 335 246
Cuts at: 2934 3946 4032 4044 4390 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 655 606 467 449 374 346 335
246 227 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1195 1195
Size: 4838
BtgI C'CryG_G
Cuts at: 107 219 269 392 686 107
Size: 112 50 123 294 4259
Fragments arranged by size:
4259 294 123 112 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 798 1488 1506 2788 2969 3132 798
Size: 690 18 1282 181 163 2504
Fragments arranged by size:
2504 1282 690 181 163 18
BtsI GCAGTG_nn'
Cuts at: 382 1427 1763 2081 382
Size: 1045 336 318 3139
Fragments arranged by size:
3139 1045 336 318
DraI TTT'AAA
Cuts at: 1837 1837
Size: 4838
DraIII CAC_nnn'GTG
Cuts at: 1464 1464
Size: 4838
DrdI GACnn_nn'nnGTC
Cuts at: 2544 3402 2544
Size: 858 3980
EaeI y'GGCC_r
Cuts at: 305 389 619 1109 1148 1187 2423 2597 2988
Size: 84 230 490 39 39 1236 174 391
Cuts at: 2988 3015 4360 305
Size: 27 1345 783
Fragments arranged by size:
1345 1236 783 490 391 230 174 84
39 39 27
EagI C'GGCC_G
Cuts at: 389 619 2423 389
Size: 230 1804 2804
Fragments arranged by size:
2804 1804 230
EarI CTCTTCn'nnn_
Cuts at: 781 1583 1641 1700 2861 3071 781
Size: 802 58 59 1161 210 2548
Fragments arranged by size:
2548 1161 802 210 59 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 604 1195 3046 3356 3502 4648 604
Size: 591 1851 310 146 1146 794
Fragments arranged by size:
1851 1146 794 591 310 146
EcoICRI GAG'CTC
Cuts at: 794 1650 1850 794
Size: 856 200 3782
Fragments arranged by size:
3782 856 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 484 886 957 1300 1329 1367 1622 2663
Size: 171 402 71 343 29 38 255 1041
Cuts at: 2663 3095 3842 4110 313
Size: 432 747 268 1041
Fragments arranged by size:
1041 1041 747 432 402 343 268 255
171 71 38 29
EcoNI CCTnn'n_nnAGG
Cuts at: 721 721
Size: 4838
EcoO109I rG'GnC_Cy
Cuts at: 320 321 956 1977 320
Size: 1 635 1021 3181
Fragments arranged by size:
3181 1021 635 1
EcoRI G'AATT_C
Cuts at: 1842 1842
Size: 4838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 4838
FseI GG_CCGG'CC
Cuts at: 805 805
Size: 4838
FspI TGC'GCA
Cuts at: 2619 2619
Size: 4838
HaeII r_GCGC'y
Cuts at: 120 183 762 1730 2272 2520 3542 4521 120
Size: 63 579 968 542 248 1022 979 437
Fragments arranged by size:
1022 979 968 579 542 437 248 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 631 663 1295 1327 2680 2710 2712 2742 631
Size: 32 632 32 1353 30 2 30 2727
Fragments arranged by size:
2727 1353 632 32 32 30 30 2
HincII GTy'rAC
Cuts at: 627 1806 1877 627
Size: 1179 71 3588
Fragments arranged by size:
3588 1179 71
KasI G'GCGC_C
Cuts at: 2268 2516 2268
Size: 248 4590
KpnI G_GTAC'C
Cuts at: 1142 1858 1142
Size: 716 4122
MluI A'CGCG_T
Cuts at: 4271 4271
Size: 4838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1574 1667 3509 3693 1574
Size: 93 1842 184 2719
Fragments arranged by size:
2719 1842 184 93
MscI TGG'CCA
Cuts at: 1111 1189 2599 1111
Size: 78 1410 3350
Fragments arranged by size:
3350 1410 78
MslI CAynn'nnrTG
Cuts at: 211 268 1609 2827 2954 211
Size: 57 1341 1218 127 2095
Fragments arranged by size:
2095 1341 1218 127 57
MspA1I CmG'CkG
Cuts at: 194 372 394 462 672 1949 2263 2623 3636
Size: 178 22 68 210 1277 314 360 1013
Cuts at: 3636 3881 194
Size: 245 1151
Fragments arranged by size:
1277 1151 1013 360 314 245 210 178
68 22
NaeI GCC'GGC
Cuts at: 389 619 803 939 3019 389
Size: 230 184 136 2080 2208
Fragments arranged by size:
2208 2080 230 184 136
NarI GG'CG_CC
Cuts at: 2269 2517 2269
Size: 248 4590
NcoI C'CATG_G
Cuts at: 107 686 107
Size: 579 4259
NgoMIV G'CCGG_C
Cuts at: 387 617 801 937 3017 387
Size: 230 184 136 2080 2208
Fragments arranged by size:
2208 2080 230 184 136
NruI TCG'CGA
Cuts at: 1080 1080
Size: 4838
NspI r_CATG'y
Cuts at: 1608 1891 2391 3298 1608
Size: 283 500 907 3148
Fragments arranged by size:
3148 907 500 283
PciI A'CATG_T
Cuts at: 3294 3294
Size: 4838
PflMI CCAn_nnn'nTGG
Cuts at: 210 291 210
Size: 81 4757
PfoI T'CCnGG_A
Cuts at: 1400 1400
Size: 4838
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4838
PmlI CAC'GTG
Cuts at: 1611 1611
Size: 4838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 4806
PpuMI rG'GwC_Cy
Cuts at: 956 956
Size: 4838
PspOMI G'GGCC_C
Cuts at: 320 320
Size: 4838
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 208 240 208
Size: 32 4806
PstI C_TGCA'G
Cuts at: 1262 1885 2570 1262
Size: 623 685 3530
Fragments arranged by size:
3530 685 623
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4838
PvuII CAG'CTG
Cuts at: 462 672 2263 2623 462
Size: 210 1591 360 2677
Fragments arranged by size:
2677 1591 360 210
RsrII CG'GwC_CG
Cuts at: 3033 3033
Size: 4838
SacI G_AGCT'C
Cuts at: 796 1652 1852 796
Size: 856 200 3782
Fragments arranged by size:
3782 856 200
SacII CC_GC'GG
Cuts at: 395 395
Size: 4838
SalI G'TCGA_C
Cuts at: 1875 1875
Size: 4838
SanDI GG'GwC_CC
Cuts at: 956 956
Size: 4838
SapI GCTCTTCn'nnn_
Cuts at: 781 1583 2861 3071 781
Size: 802 1278 210 2548
Fragments arranged by size:
2548 1278 802 210
SbfI CC_TGCA'GG
Cuts at: 1885 1885
Size: 4838
ScaI AGT'ACT
Cuts at: 547 4355 547
Size: 3808 1030
SfcI C'TryA_G
Cuts at: 33 1258 1881 2566 3559 3750 33
Size: 1225 623 685 993 191 1121
Fragments arranged by size:
1225 1121 993 685 623 191
SfoI GGC'GCC
Cuts at: 2270 2518 2270
Size: 248 4590
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
SgrAI Cr'CCGG_yG
Cuts at: 1507 1507
Size: 4838
SmaI CCC'GGG
Cuts at: 279 808 1860 279
Size: 529 1052 3257
Fragments arranged by size:
3257 1052 529
SmlI C'TyrA_G
Cuts at: 329 1993 2908 3400 3662 329
Size: 1664 915 492 262 1505
Fragments arranged by size:
1664 1505 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4838
SphI G_CATG'C
Cuts at: 1891 2391 1891
Size: 500 4338
StyI C'CwwG_G
Cuts at: 107 686 1250 1972 2326 107
Size: 579 564 722 354 2619
Fragments arranged by size:
2619 722 579 564 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1475 2421 2554 3013 3222 1475
Size: 946 133 459 209 3091
Fragments arranged by size:
3091 946 459 209 133
TatI w'GTAC_w
Cuts at: 545 1601 4353 545
Size: 1056 2752 1030
Fragments arranged by size:
2752 1056 1030
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 994 1026 4355 4387 994
Size: 32 3329 32 1445
Fragments arranged by size:
3329 1445 32 32
Tth111I GACn'n_nGTC
Cuts at: 2635 2635
Size: 4838
XbaI T'CTAG_A
Cuts at: 1869 1869
Size: 4838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1296 1437 2333 1296
Size: 141 896 3801
Fragments arranged by size:
3801 896 141
XmaI C'CCGG_G
Cuts at: 277 806 1858 277
Size: 529 1052 3257
Fragments arranged by size:
3257 1052 529
ZraI GAC'GTC
Cuts at: 914 914
Size: 4838
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI AscI AvrII BaeI BfrBI
BglI BmtI BsiWI BsmI BsrGI BssHII BstZ17I ClaI
CspCI EcoRV FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PasI PshAI PsiI PspXI SexAI
SfiI SnaBI SrfI SspI StuI SwaI XhoI XmnI