Restriction map for
pF1KB9877
(Circular) (Six-base) MAPSORT of: pF1KB9877.seq Check: 22 from: 1 to: 4751
pF1KB9877 4751 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 141 141
Size: 4751
AccI GT'mk_AC
Cuts at: 1789 1789
Size: 4751
Acc65I G'GTAC_C
Cuts at: 1767 1767
Size: 4751
AclI AA'CG_TT
Cuts at: 4591 4591
Size: 4751
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 2576 3008 3755 4023 400
Size: 2176 432 747 268 1128
Fragments arranged by size:
2176 1128 747 432 268
AfeI AGC'GCT
Cuts at: 387 400 387
Size: 13 4738
AflIII A'CryG_T
Cuts at: 2103 3207 4184 2103
Size: 1104 977 2670
Fragments arranged by size:
2670 1104 977
AgeI A'CCGG_T
Cuts at: 3139 3139
Size: 4751
AlwNI CAG_nnn'CTG
Cuts at: 1386 2056 3623 1386
Size: 670 1567 2514
Fragments arranged by size:
2514 1567 670
ApaI G_GGCC'C
Cuts at: 658 658
Size: 4751
ApaLI G'TGCA_C
Cuts at: 3521 3521
Size: 4751
ApoI r'AATT_y
Cuts at: 1755 4104 4360 1755
Size: 2349 256 2146
Fragments arranged by size:
2349 2146 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4751
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4751
AvaI C'yCGr_G
Cuts at: 537 649 673 1077 1265 1373 1594 1771 537
Size: 112 24 404 188 108 221 177 3517
Fragments arranged by size:
3517 404 221 188 177 112 108 24
AvrII C'CTAG_G
Cuts at: 1141 1141
Size: 4751
BamHI G'GATC_C
Cuts at: 1776 1776
Size: 4751
BanI G'GyrC_C
Cuts at: 891 1107 1137 1650 1767 2181 2429 2464 891
Size: 216 30 513 117 414 248 35 3178
Fragments arranged by size:
3178 513 414 248 216 117 35 30
BanII G_rGCy'C
Cuts at: 472 560 658 665 917 1084 1765 2795 472
Size: 88 98 7 252 167 681 1030 2428
Fragments arranged by size:
2428 1030 681 252 167 98 88 7
BbeI G_GCGC'C
Cuts at: 1654 2185 2433 1654
Size: 531 248 3972
Fragments arranged by size:
3972 531 248
BbsI GAAGACnn'nnnn_
Cuts at: 897 897
Size: 4751
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 873 907 873
Size: 34 4717
BciVI GTATCCnnnnn_n'
Cuts at: 2649 3410 4751 2649
Size: 761 1341 2649
Fragments arranged by size:
2649 1341 761
BclI T'GATC_A
Cuts at: 2271 2271
Size: 4751
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4751
BglII A'GATC_T
Cuts at: 2266 4303 2266
Size: 2037 2714
BlpI GC'TnA_GC
Cuts at: 1863 1863
Size: 4751
Bme1580I G_kGCm'C
Cuts at: 658 674 788 896 2376 2469 3525 658
Size: 16 114 108 1480 93 1056 1884
Fragments arranged by size:
1884 1480 1056 114 108 93 16
BmgBI CAC'GTC
Cuts at: 1170 1170
Size: 4751
BmrI ACTGGGnnnn_n'
Cuts at: 542 1963 2044 2379 4523 542
Size: 1421 81 335 2144 770
Fragments arranged by size:
2144 1421 770 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 491 3118 491
Size: 2627 2124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1068 1087 1285 1006
Size: 62 19 198 4472
Fragments arranged by size:
4472 198 62 19
Bpu10I CC'TnA_GC
Cuts at: 485 659 710 485
Size: 174 51 4526
Fragments arranged by size:
4526 174 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1927 2806 3298 3596 1927
Size: 879 492 298 3082
Fragments arranged by size:
3082 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 522 548 4455 522
Size: 26 3907 818
Fragments arranged by size:
3907 818 26
BsaAI yAC'GTr
Cuts at: 291 1420 2104 291
Size: 1129 684 2938
Fragments arranged by size:
2938 1129 684
BsaBI GATnn'nnATC
Cuts at: 6 2265 4302 6
Size: 2259 2037 455
Fragments arranged by size:
2259 2037 455
BsaHI Gr'CG_yC
Cuts at: 1157 1219 1370 1480 1651 2182 2430 3132 4628
Size: 62 151 110 171 531 248 702 1496
Cuts at: 4628 1157
Size: 1280
Fragments arranged by size:
1496 1280 702 531 248 171 151 110
62
BsaWI w'CCGG_w
Cuts at: 1152 1940 2461 3139 3413 3560 3971 1152
Size: 788 521 678 274 147 411 1932
Fragments arranged by size:
1932 788 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1066 1096 1321 1351 1066
Size: 30 225 30 4466
Fragments arranged by size:
4466 225 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 394 394
Size: 4751
BseYI C'CCAG_C
Cuts at: 591 726 895 1432 2176 2934 3511 4007 591
Size: 135 169 537 744 758 577 496 1335
Fragments arranged by size:
1335 758 744 577 537 496 169 135
BsiEI CG_ry'CG
Cuts at: 104 863 1440 2293 2339 3547 104
Size: 759 577 853 46 1208 1308
Fragments arranged by size:
1308 1208 853 759 577 46
BsiHKAI G_wGCw'C
Cuts at: 341 491 917 1334 1765 2543 2733 3525 341
Size: 150 426 417 431 778 190 792 1567
Fragments arranged by size:
1567 792 778 431 426 417 190 150
Bsp1286I G_dGCh'C
Cuts at: 341 472 491 560 658 665 674 788 896
Size: 131 19 69 98 7 9 114 108
Cuts at: 896 917 1084 1334 1765 2376 2469 2543 2733
Size: 21 167 250 431 611 93 74 190
Cuts at: 2733 2795 3525 341
Size: 62 730 1567
Fragments arranged by size:
1567 730 611 431 250 190 167 131
114 108 98 93 74 69 62 21
19 9 7
BspEI T'CCGG_A
Cuts at: 1152 3971 1152
Size: 2819 1932
BspHI T'CATG_A
Cuts at: 3927 3927
Size: 4751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 141 1364 1801 2015 2317 2698 141
Size: 1223 437 214 302 381 2194
Fragments arranged by size:
2194 1223 437 381 302 214
BsrBI CCG'CTC
Cuts at: 50 423 634 709 1622 3043 3097 4581 4746
Size: 373 211 75 913 1421 54 1484 165
Cuts at: 4746 50
Size: 55
Fragments arranged by size:
1484 1421 913 373 211 165 75 55
54
BsrDI GCAATG_nn'
Cuts at: 1293 2663 1293
Size: 1370 3381
BsrFI r'CCGG_y
Cuts at: 862 1068 1640 2749 2930 3139 862
Size: 206 572 1109 181 209 2474
Fragments arranged by size:
2474 1109 572 209 206 181
BssHII G'CGCG_C
Cuts at: 132 132
Size: 4751
BssSI C'ACGA_G
Cuts at: 340 1333 3022 3380 340
Size: 993 1689 358 1711
Fragments arranged by size:
1711 1689 993 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1386 2128 1386
Size: 742 4009
BstBI TT'CG_AA
Cuts at: 3112 3112
Size: 4751
BstXI CCAn_nnnn'nTGG
Cuts at: 1689 1689
Size: 4751
BstYI r'GATC_y
Cuts at: 1 1776 2243 2266 2601 2847 3859 3945 3957
Size: 1775 467 23 335 246 1012 86 12
Cuts at: 3957 4303 1
Size: 346 449
Fragments arranged by size:
1775 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 377 377
Size: 4751
BtgI C'CryG_G
Cuts at: 107 798 1655 107
Size: 691 857 3203
Fragments arranged by size:
3203 857 691
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2701 2882 3045 2701
Size: 181 163 4407
Fragments arranged by size:
4407 181 163
BtsI GCAGTG_nn'
Cuts at: 143 1132 1994 143
Size: 989 862 2900
Fragments arranged by size:
2900 989 862
DraI TTT'AAA
Cuts at: 1750 1750
Size: 4751
DrdI GACnn_nn'nnGTC
Cuts at: 2457 3315 2457
Size: 858 3893
EaeI y'GGCC_r
Cuts at: 161 860 1437 1472 1703 2336 2510 2901 2928
Size: 699 577 35 231 633 174 391 27
Cuts at: 2928 4273 161
Size: 1345 639
Fragments arranged by size:
1345 699 639 633 577 391 231 174
35 27
EagI C'GGCC_G
Cuts at: 860 1437 2336 860
Size: 577 899 3275
Fragments arranged by size:
3275 899 577
EarI CTCTTCn'nnn_
Cuts at: 419 684 2774 2984 419
Size: 265 2090 210 2186
Fragments arranged by size:
2186 2090 265 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 201 256 864 2959 3269 3415 4561 201
Size: 55 608 2095 310 146 1146 391
Fragments arranged by size:
2095 1146 608 391 310 146 55
EcoICRI GAG'CTC
Cuts at: 915 1763 915
Size: 848 3903
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 1006 1068 1087 1285 2576 3008 3755 4023
Size: 606 62 19 198 1291 432 747 268
Cuts at: 4023 400
Size: 1128
Fragments arranged by size:
1291 1128 747 606 432 268 198 62
19
EcoO109I rG'GnC_Cy
Cuts at: 372 615 654 655 921 1179 1890 372
Size: 243 39 1 266 258 711 3233
Fragments arranged by size:
3233 711 266 258 243 39 1
EcoRI G'AATT_C
Cuts at: 1755 1755
Size: 4751
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 4751
FspI TGC'GCA
Cuts at: 2532 2532
Size: 4751
HaeII r_GCGC'y
Cuts at: 273 389 402 579 869 1654 2185 2433 3455
Size: 116 13 177 290 785 531 248 1022
Cuts at: 3455 4434 273
Size: 979 590
Fragments arranged by size:
1022 979 785 590 531 290 248 177
116 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 946 978 1209 1241 1321 1353 2593 2623 2625
Size: 32 231 32 80 32 1240 30 2
Cuts at: 2625 2655 946
Size: 30 3042
Fragments arranged by size:
3042 1240 231 80 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1790 1790
Size: 4751
KasI G'GCGC_C
Cuts at: 1650 2181 2429 1650
Size: 531 248 3972
Fragments arranged by size:
3972 531 248
KpnI G_GTAC'C
Cuts at: 1771 1771
Size: 4751
MluI A'CGCG_T
Cuts at: 4184 4184
Size: 4751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 646 3422 3606 646
Size: 2776 184 1791
Fragments arranged by size:
2776 1791 184
MscI TGG'CCA
Cuts at: 163 1474 1705 2512 163
Size: 1311 231 807 2402
Fragments arranged by size:
2402 1311 807 231
MslI CAynn'nnrTG
Cuts at: 2740 2867 2740
Size: 127 4624
MspA1I CmG'CkG
Cuts at: 595 667 800 1383 1436 1525 1657 1698 1862
Size: 72 133 583 53 89 132 41 164
Cuts at: 1862 2176 2536 3549 3794 595
Size: 314 360 1013 245 1552
Fragments arranged by size:
1552 1013 583 360 314 245 164 133
132 89 72 53 41
NaeI GCC'GGC
Cuts at: 864 2932 864
Size: 2068 2683
NarI GG'CG_CC
Cuts at: 1651 2182 2430 1651
Size: 531 248 3972
Fragments arranged by size:
3972 531 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4751
NgoMIV G'CCGG_C
Cuts at: 862 2930 862
Size: 2068 2683
NruI TCG'CGA
Cuts at: 554 554
Size: 4751
NspI r_CATG'y
Cuts at: 1804 2304 3211 1804
Size: 500 907 3344
Fragments arranged by size:
3344 907 500
PasI CC'CwG_GG
Cuts at: 753 753
Size: 4751
PciI A'CATG_T
Cuts at: 3207 3207
Size: 4751
PflMI CCAn_nnn'nTGG
Cuts at: 291 291
Size: 4751
PfoI T'CCnGG_A
Cuts at: 830 1022 1301 1495 830
Size: 192 279 194 4086
Fragments arranged by size:
4086 279 194 192
PmeI GTTT'AAAC
Cuts at: 1750 1750
Size: 4751
PmlI CAC'GTG
Cuts at: 291 1420 291
Size: 1129 3622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 3890
Size: 32 4719
PpuMI rG'GwC_Cy
Cuts at: 372 615 921 1179 372
Size: 243 306 258 3944
Fragments arranged by size:
3944 306 258 243
PspOMI G'GGCC_C
Cuts at: 654 654
Size: 4751
PstI C_TGCA'G
Cuts at: 152 857 1518 1798 2483 152
Size: 705 661 280 685 2420
Fragments arranged by size:
2420 705 685 661 280
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4751
PvuII CAG'CTG
Cuts at: 595 2176 2536 595
Size: 1581 360 2810
Fragments arranged by size:
2810 1581 360
RsrII CG'GwC_CG
Cuts at: 2946 2946
Size: 4751
SacI G_AGCT'C
Cuts at: 917 1765 917
Size: 848 3903
SacII CC_GC'GG
Cuts at: 801 1658 801
Size: 857 3894
SalI G'TCGA_C
Cuts at: 1788 1788
Size: 4751
SanDI GG'GwC_CC
Cuts at: 615 921 1179 615
Size: 306 258 4187
Fragments arranged by size:
4187 306 258
SapI GCTCTTCn'nnn_
Cuts at: 419 2774 2984 419
Size: 2355 210 2186
Fragments arranged by size:
2355 2186 210
SbfI CC_TGCA'GG
Cuts at: 1798 1798
Size: 4751
ScaI AGT'ACT
Cuts at: 4268 4268
Size: 4751
SfcI C'TryA_G
Cuts at: 33 148 853 1514 1794 2479 3472 3663 33
Size: 115 705 661 280 685 993 191 1121
Fragments arranged by size:
1121 993 705 685 661 280 191 115
SfoI GGC'GCC
Cuts at: 1652 2183 2431 1652
Size: 531 248 3972
Fragments arranged by size:
3972 531 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4751
SmaI CCC'GGG
Cuts at: 675 1773 675
Size: 1098 3653
SmlI C'TyrA_G
Cuts at: 1265 1906 2821 3313 3575 1265
Size: 641 915 492 262 2441
Fragments arranged by size:
2441 915 641 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4751
SphI G_CATG'C
Cuts at: 1804 2304 1804
Size: 500 4251
SspI AAT'ATT
Cuts at: 196 196
Size: 4751
StuI AGG'CCT
Cuts at: 433 934 1089 433
Size: 501 155 4095
Fragments arranged by size:
4095 501 155
StyI C'CwwG_G
Cuts at: 107 1141 1706 1885 2239 107
Size: 1034 565 179 354 2619
Fragments arranged by size:
2619 1034 565 354 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 362 2334 2467 2926 3135 362
Size: 1972 133 459 209 1978
Fragments arranged by size:
1978 1972 459 209 133
TatI w'GTAC_w
Cuts at: 1073 4266 1073
Size: 3193 1558
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 778 810 1037 1069 4268 4300 778
Size: 32 227 32 3199 32 1229
Fragments arranged by size:
3199 1229 227 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2548 2548
Size: 4751
XbaI T'CTAG_A
Cuts at: 1782 1782
Size: 4751
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2246 2246
Size: 4751
XhoI C'TCGA_G
Cuts at: 1265 1265
Size: 4751
XmaI C'CCGG_G
Cuts at: 673 1771 673
Size: 1098 3653
XmnI GAAnn'nnTTC
Cuts at: 1202 1202
Size: 4751
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI BaeI
BbvCI BfrBI BmtI BsgI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SwaI ZraI