Restriction map for
pF1KB9873
(Circular) (Six-base) MAPSORT of: pF1KB9873.seq Check: 1919 from: 1 to: 4610
pF1KB9873 4610 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1288 1288
Size: 4610
AccI GT'mk_AC
Cuts at: 1648 1648
Size: 4610
Acc65I G'GTAC_C
Cuts at: 740 1626 740
Size: 886 3724
AclI AA'CG_TT
Cuts at: 4450 4450
Size: 4610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 2435 2867 3614 3882 458
Size: 1977 432 747 268 1186
Fragments arranged by size:
1977 1186 747 432 268
AflIII A'CryG_T
Cuts at: 1962 3066 4043 1962
Size: 1104 977 2529
Fragments arranged by size:
2529 1104 977
AgeI A'CCGG_T
Cuts at: 1320 2998 1320
Size: 1678 2932
AhdI GACnn_n'nnGTC
Cuts at: 1409 1409
Size: 4610
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1232 1264 1232
Size: 32 4578
AlwNI CAG_nnn'CTG
Cuts at: 589 647 1915 3482 589
Size: 58 1268 1567 1717
Fragments arranged by size:
1717 1567 1268 58
ApaLI G'TGCA_C
Cuts at: 3380 3380
Size: 4610
ApoI r'AATT_y
Cuts at: 443 814 1614 3963 4219 443
Size: 371 800 2349 256 834
Fragments arranged by size:
2349 834 800 371 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4610
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
AvaI C'yCGr_G
Cuts at: 1630 1630
Size: 4610
BamHI G'GATC_C
Cuts at: 1635 1635
Size: 4610
BanI G'GyrC_C
Cuts at: 158 740 1626 2040 2288 2323 158
Size: 582 886 414 248 35 2445
Fragments arranged by size:
2445 886 582 414 248 35
BanII G_rGCy'C
Cuts at: 583 1624 2654 583
Size: 1041 1030 2539
Fragments arranged by size:
2539 1041 1030
BbeI G_GCGC'C
Cuts at: 2044 2292 2044
Size: 248 4362
BbsI GAAGACnn'nnnn_
Cuts at: 692 692
Size: 4610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 127 161 127
Size: 34 4576
BciVI GTATCCnnnnn_n'
Cuts at: 2508 3269 4610 2508
Size: 761 1341 2508
Fragments arranged by size:
2508 1341 761
BclI T'GATC_A
Cuts at: 215 2130 215
Size: 1915 2695
BfrBI ATG'CAT
Cuts at: 693 693
Size: 4610
BglII A'GATC_T
Cuts at: 2125 4162 2125
Size: 2037 2573
BlpI GC'TnA_GC
Cuts at: 950 1722 950
Size: 772 3838
Bme1580I G_kGCm'C
Cuts at: 161 422 2235 2328 3384 161
Size: 261 1813 93 1056 1387
Fragments arranged by size:
1813 1387 1056 261 93
BmrI ACTGGGnnnn_n'
Cuts at: 258 1170 1822 1903 2238 4382 258
Size: 912 652 81 335 2144 486
Fragments arranged by size:
2144 912 652 486 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 4610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1033
Size: 4610
Bpu10I CC'TnA_GC
Cuts at: 258 582 258
Size: 324 4286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1786 2665 3157 3455 1786
Size: 879 492 298 2941
Fragments arranged by size:
2941 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 119 235 268 1051 4314 119
Size: 116 33 783 3263 415
Fragments arranged by size:
3263 783 415 116 33
BsaAI yAC'GTr
Cuts at: 1963 1963
Size: 4610
BsaBI GATnn'nnATC
Cuts at: 6 220 628 2124 4161 6
Size: 214 408 1496 2037 455
Fragments arranged by size:
2037 1496 455 408 214
BsaHI Gr'CG_yC
Cuts at: 2041 2289 2991 4487 2041
Size: 248 702 1496 2164
Fragments arranged by size:
2164 1496 702 248
BsaWI w'CCGG_w
Cuts at: 121 995 1320 1799 2320 2998 3272 3419 3830
Size: 874 325 479 521 678 274 147 411
Cuts at: 3830 121
Size: 901
Fragments arranged by size:
901 874 678 521 479 411 325 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1232 1262 1232
Size: 30 4580
BseYI C'CCAG_C
Cuts at: 129 245 459 841 1183 2035 2793 3370 3866
Size: 116 214 382 342 852 758 577 496
Cuts at: 3866 129
Size: 873
Fragments arranged by size:
873 852 758 577 496 382 342 214
116
BsiEI CG_ry'CG
Cuts at: 104 345 2152 2198 3406 104
Size: 241 1807 46 1208 1308
Fragments arranged by size:
1807 1308 1208 241 46
BsiHKAI G_wGCw'C
Cuts at: 583 1624 2402 2592 3384 583
Size: 1041 778 190 792 1809
Fragments arranged by size:
1809 1041 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1221 1221
Size: 4610
Bsp1286I G_dGCh'C
Cuts at: 161 422 583 1624 2235 2328 2402 2592 2654
Size: 261 161 1041 611 93 74 190 62
Cuts at: 2654 3384 161
Size: 730 1387
Fragments arranged by size:
1387 1041 730 611 261 190 161 93
74 62
BspEI T'CCGG_A
Cuts at: 3830 3830
Size: 4610
BspHI T'CATG_A
Cuts at: 869 3786 869
Size: 2917 1693
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1288 1660 1874 2176 2557 1288
Size: 372 214 302 381 3341
Fragments arranged by size:
3341 381 372 302 214
BsrBI CCG'CTC
Cuts at: 50 2902 2956 4440 4605 50
Size: 2852 54 1484 165 55
Fragments arranged by size:
2852 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 658 2522 658
Size: 1864 2746
BsrFI r'CCGG_y
Cuts at: 850 1320 2608 2789 2998 850
Size: 470 1288 181 209 2462
Fragments arranged by size:
2462 1288 470 209 181
BssSI C'ACGA_G
Cuts at: 193 2881 3239 193
Size: 2688 358 1564
Fragments arranged by size:
2688 1564 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1987 1987
Size: 4610
BstBI TT'CG_AA
Cuts at: 2971 2971
Size: 4610
BstEII G'GTnAC_C
Cuts at: 705 705
Size: 4610
BstXI CCAn_nnnn'nTGG
Cuts at: 114 975 114
Size: 861 3749
BstYI r'GATC_y
Cuts at: 1 1418 1487 1635 2102 2125 2460 2706 3718
Size: 1417 69 148 467 23 335 246 1012
Cuts at: 3718 3804 3816 4162 1
Size: 86 12 346 449
Fragments arranged by size:
1417 1012 467 449 346 335 246 148
86 69 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 2560 2741 2904 487
Size: 2073 181 163 2193
Fragments arranged by size:
2193 2073 181 163
BtsI GCAGTG_nn'
Cuts at: 1853 1853
Size: 4610
DraI TTT'AAA
Cuts at: 1609 1609
Size: 4610
DrdI GACnn_nn'nnGTC
Cuts at: 2316 3174 2316
Size: 858 3752
EaeI y'GGCC_r
Cuts at: 116 890 977 2195 2369 2760 2787 4132 116
Size: 774 87 1218 174 391 27 1345 594
Fragments arranged by size:
1345 1218 774 594 391 174 87 27
EagI C'GGCC_G
Cuts at: 2195 2195
Size: 4610
EarI CTCTTCn'nnn_
Cuts at: 509 1117 2633 2843 509
Size: 608 1516 210 2276
Fragments arranged by size:
2276 1516 608 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2818 3128 3274 4420 2818
Size: 310 146 1146 3008
Fragments arranged by size:
3008 1146 310 146
EcoICRI GAG'CTC
Cuts at: 581 1622 581
Size: 1041 3569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 1033 2435 2867 3614 3882 458
Size: 575 1402 432 747 268 1186
Fragments arranged by size:
1402 1186 747 575 432 268
EcoO109I rG'GnC_Cy
Cuts at: 288 586 1749 288
Size: 298 1163 3149
Fragments arranged by size:
3149 1163 298
EcoRI G'AATT_C
Cuts at: 814 1614 814
Size: 800 3810
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 574 2629 574
Size: 2055 2555
FspI TGC'GCA
Cuts at: 2391 2391
Size: 4610
HaeII r_GCGC'y
Cuts at: 2044 2292 3314 4293 2044
Size: 248 1022 979 2361
Fragments arranged by size:
2361 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 1044 1076 1156 1188 1395 1427 2452
Size: 32 594 32 80 32 207 32 1025
Cuts at: 2452 2482 2484 2514 418
Size: 30 2 30 2514
Fragments arranged by size:
2514 1025 594 207 80 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1307 1595 1649 1307
Size: 288 54 4268
Fragments arranged by size:
4268 288 54
HpaI GTT'AAC
Cuts at: 1307 1595 1307
Size: 288 4322
KasI G'GCGC_C
Cuts at: 2040 2288 2040
Size: 248 4362
KpnI G_GTAC'C
Cuts at: 744 1630 744
Size: 886 3724
MfeI C'AATT_G
Cuts at: 635 635
Size: 4610
MluI A'CGCG_T
Cuts at: 4043 4043
Size: 4610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 749 1276 3281 3465 749
Size: 527 2005 184 1894
Fragments arranged by size:
2005 1894 527 184
MscI TGG'CCA
Cuts at: 118 892 979 2371 118
Size: 774 87 1392 2357
Fragments arranged by size:
2357 1392 774 87
MslI CAynn'nnrTG
Cuts at: 112 698 2599 2726 112
Size: 586 1901 127 1996
Fragments arranged by size:
1996 1901 586 127
MspA1I CmG'CkG
Cuts at: 1721 2035 2395 3408 3653 1721
Size: 314 360 1013 245 2678
Fragments arranged by size:
2678 1013 360 314 245
NaeI GCC'GGC
Cuts at: 852 2791 852
Size: 1939 2671
NarI GG'CG_CC
Cuts at: 2041 2289 2041
Size: 248 4362
NgoMIV G'CCGG_C
Cuts at: 850 2789 850
Size: 1939 2671
NsiI A_TGCA'T
Cuts at: 695 695
Size: 4610
NspI r_CATG'y
Cuts at: 693 1663 2163 3070 693
Size: 970 500 907 2233
Fragments arranged by size:
2233 970 907 500
PasI CC'CwG_GG
Cuts at: 154 915 154
Size: 761 3849
PciI A'CATG_T
Cuts at: 3066 3066
Size: 4610
PfoI T'CCnGG_A
Cuts at: 671 1490 671
Size: 819 3791
PmeI GTTT'AAAC
Cuts at: 1609 1609
Size: 4610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1162 1194 1232 1264 3749 3781 1162
Size: 32 38 32 2485 32 1991
Fragments arranged by size:
2485 1991 38 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 288 288
Size: 4610
PstI C_TGCA'G
Cuts at: 1657 2342 1657
Size: 685 3925
PvuI CG_AT'CG
Cuts at: 104 345 104
Size: 241 4369
PvuII CAG'CTG
Cuts at: 2035 2395 2035
Size: 360 4250
RsrII CG'GwC_CG
Cuts at: 2805 2805
Size: 4610
SacI G_AGCT'C
Cuts at: 583 1624 583
Size: 1041 3569
SalI G'TCGA_C
Cuts at: 1647 1647
Size: 4610
SanDI GG'GwC_CC
Cuts at: 288 288
Size: 4610
SapI GCTCTTCn'nnn_
Cuts at: 509 2633 2843 509
Size: 2124 210 2276
Fragments arranged by size:
2276 2124 210
SbfI CC_TGCA'GG
Cuts at: 1657 1657
Size: 4610
ScaI AGT'ACT
Cuts at: 334 4127 334
Size: 3793 817
SfcI C'TryA_G
Cuts at: 33 1229 1653 2338 3331 3522 33
Size: 1196 424 685 993 191 1121
Fragments arranged by size:
1196 1121 993 685 424 191
SfoI GGC'GCC
Cuts at: 2042 2290 2042
Size: 248 4362
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
SmaI CCC'GGG
Cuts at: 1632 1632
Size: 4610
SmlI C'TyrA_G
Cuts at: 1765 2680 3172 3434 1765
Size: 915 492 262 2941
Fragments arranged by size:
2941 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4610
SphI G_CATG'C
Cuts at: 1663 2163 1663
Size: 500 4110
SspI AAT'ATT
Cuts at: 1581 1581
Size: 4610
StyI C'CwwG_G
Cuts at: 1556 1744 2098 1556
Size: 188 354 4068
Fragments arranged by size:
4068 354 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 176 2193 2326 2785 2994 176
Size: 2017 133 459 209 1792
Fragments arranged by size:
2017 1792 459 209 133
TatI w'GTAC_w
Cuts at: 332 374 4125 332
Size: 42 3751 817
Fragments arranged by size:
3751 817 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 706 738 965 997 1259 1291 4127 4159 706
Size: 32 227 32 262 32 2836 32 1157
Fragments arranged by size:
2836 1157 262 227 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2407 2407
Size: 4610
XbaI T'CTAG_A
Cuts at: 1641 1641
Size: 4610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1199 1554 2105 1199
Size: 355 551 3704
Fragments arranged by size:
3704 551 355
XmaI C'CCGG_G
Cuts at: 1630 1630
Size: 4610
XmnI GAAnn'nnTTC
Cuts at: 443 443
Size: 4610
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI ApaI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI BseRI BsgI BsiWI
BsmI BsrGI BssHII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII NcoI NdeI
NheI NotI NruI PacI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI