Restriction map for
pF1KB9871
(Circular) (Six-base) MAPSORT of: pF1KB9871.seq Check: 5045 from: 1 to: 4583
pF1KB9871 4583 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 926 1108 1244 1334 926
Size: 182 136 90 4175
Fragments arranged by size:
4175 182 136 90
AccI GT'mk_AC
Cuts at: 1621 1621
Size: 4583
Acc65I G'GTAC_C
Cuts at: 1599 1599
Size: 4583
AclI AA'CG_TT
Cuts at: 4423 4423
Size: 4583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 2408 2840 3587 3855 879
Size: 1529 432 747 268 1607
Fragments arranged by size:
1607 1529 747 432 268
AfeI AGC'GCT
Cuts at: 1032 1294 1351 1378 1032
Size: 262 57 27 4237
Fragments arranged by size:
4237 262 57 27
AflIII A'CryG_T
Cuts at: 1935 3039 4016 1935
Size: 1104 977 2502
Fragments arranged by size:
2502 1104 977
AgeI A'CCGG_T
Cuts at: 2971 2971
Size: 4583
AleI CACnn'nnGTG
Cuts at: 610 610
Size: 4583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4583
AlwNI CAG_nnn'CTG
Cuts at: 1888 3455 1888
Size: 1567 3016
ApaI G_GGCC'C
Cuts at: 1141 1141
Size: 4583
ApaLI G'TGCA_C
Cuts at: 601 3353 601
Size: 2752 1831
ApoI r'AATT_y
Cuts at: 1587 3936 4192 1587
Size: 2349 256 1978
Fragments arranged by size:
2349 1978 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4583
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
AvaI C'yCGr_G
Cuts at: 687 1188 1455 1549 1555 1603 687
Size: 501 267 94 6 48 3667
Fragments arranged by size:
3667 501 267 94 48 6
BamHI G'GATC_C
Cuts at: 1608 1608
Size: 4583
BanI G'GyrC_C
Cuts at: 724 904 1044 1322 1559 1599 2013 2261 2296
Size: 180 140 278 237 40 414 248 35
Cuts at: 2296 724
Size: 3011
Fragments arranged by size:
3011 414 278 248 237 180 140 40
35
BanII G_rGCy'C
Cuts at: 718 827 1021 1060 1141 1456 1556 1597 2627
Size: 109 194 39 81 315 100 41 1030
Cuts at: 2627 718
Size: 2674
Fragments arranged by size:
2674 1030 315 194 109 100 81 41
39
BbeI G_GCGC'C
Cuts at: 728 908 1048 2017 2265 728
Size: 180 140 969 248 3046
Fragments arranged by size:
3046 969 248 180 140
BbsI GAAGACnn'nnnn_
Cuts at: 1420 1420
Size: 4583
BbvCI CC'TCA_GC
Cuts at: 1071 1399 1071
Size: 328 4255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4549
BciVI GTATCCnnnnn_n'
Cuts at: 2481 3242 4583 2481
Size: 761 1341 2481
Fragments arranged by size:
2481 1341 761
BclI T'GATC_A
Cuts at: 2103 2103
Size: 4583
BglII A'GATC_T
Cuts at: 2098 4135 2098
Size: 2037 2546
BlpI GC'TnA_GC
Cuts at: 922 1695 922
Size: 773 3810
Bme1580I G_kGCm'C
Cuts at: 605 1141 1562 2208 2301 3357 605
Size: 536 421 646 93 1056 1831
Fragments arranged by size:
1831 1056 646 536 421 93
BmgBI CAC'GTC
Cuts at: 744 765 744
Size: 21 4562
BmrI ACTGGGnnnn_n'
Cuts at: 1795 1876 2211 4355 1795
Size: 81 335 2144 2023
Fragments arranged by size:
2144 2023 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 732 2950 732
Size: 2218 2365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 375 396 441 714 270
Size: 105 21 45 273 4139
Fragments arranged by size:
4139 273 105 45 21
Bpu10I CC'TnA_GC
Cuts at: 1071 1399 1071
Size: 328 4255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 1210 1759 2638 3130 3428 246
Size: 964 549 879 492 298 1401
Fragments arranged by size:
1401 964 879 549 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4287 4287
Size: 4583
BsaAI yAC'GTr
Cuts at: 1410 1936 1410
Size: 526 4057
BsaBI GATnn'nnATC
Cuts at: 6 2097 4134 6
Size: 2091 2037 455
Fragments arranged by size:
2091 2037 455
BsaHI Gr'CG_yC
Cuts at: 725 905 1045 2014 2262 2964 4460 725
Size: 180 140 969 248 702 1496 848
Fragments arranged by size:
1496 969 848 702 248 180 140
BsaWI w'CCGG_w
Cuts at: 1772 2293 2971 3245 3392 3803 1772
Size: 521 678 274 147 411 2552
Fragments arranged by size:
2552 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 147 204 540 900 1059 147
Size: 57 336 360 159 3671
Fragments arranged by size:
3671 360 336 159 57
BseYI C'CCAG_C
Cuts at: 142 1033 1050 1134 2008 2766 3343 3839 142
Size: 891 17 84 874 758 577 496 886
Fragments arranged by size:
891 886 874 758 577 496 84 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 790 823 1156 787
Size: 3 33 333 4214
Fragments arranged by size:
4214 333 33 3
BsiEI CG_ry'CG
Cuts at: 104 323 722 1092 2125 2171 3379 104
Size: 219 399 370 1033 46 1208 1308
Fragments arranged by size:
1308 1208 1033 399 370 219 46
BsiHKAI G_wGCw'C
Cuts at: 605 1499 1597 2375 2565 3357 605
Size: 894 98 778 190 792 1831
Fragments arranged by size:
1831 894 792 778 190 98
BsiWI C'GTAC_G
Cuts at: 659 659
Size: 4583
BsmBI CGTCTCn'nnnn_
Cuts at: 155 155
Size: 4583
Bsp1286I G_dGCh'C
Cuts at: 605 718 827 1021 1060 1141 1456 1499 1556
Size: 113 109 194 39 81 315 43 57
Cuts at: 1556 1562 1597 2208 2301 2375 2565 2627 3357
Size: 6 35 611 93 74 190 62 730
Cuts at: 3357 605
Size: 1831
Fragments arranged by size:
1831 730 611 315 194 190 113 109
93 81 74 62 57 43 39 35
6
BspEI T'CCGG_A
Cuts at: 3803 3803
Size: 4583
BspHI T'CATG_A
Cuts at: 3759 3759
Size: 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 926 1108 1244 1334 1633 1847 2149 2530 926
Size: 182 136 90 299 214 302 381 2979
Fragments arranged by size:
2979 381 302 299 214 182 136 90
BsrBI CCG'CTC
Cuts at: 50 849 1131 1257 2875 2929 4413 4578 50
Size: 799 282 126 1618 54 1484 165 55
Fragments arranged by size:
1618 1484 799 282 165 126 55 54
BsrDI GCAATG_nn'
Cuts at: 2495 2495
Size: 4583
BsrFI r'CCGG_y
Cuts at: 582 721 751 1006 1339 2581 2762 2971 582
Size: 139 30 255 333 1242 181 209 2194
Fragments arranged by size:
2194 1242 333 255 209 181 139 30
BssSI C'ACGA_G
Cuts at: 2854 3212 2854
Size: 358 4225
BstAPI GCAn_nnn'nTGC
Cuts at: 1960 1960
Size: 4583
BstBI TT'CG_AA
Cuts at: 2944 2944
Size: 4583
BstYI r'GATC_y
Cuts at: 1 1608 2075 2098 2433 2679 3691 3777 3789
Size: 1607 467 23 335 246 1012 86 12
Cuts at: 3789 4135 1
Size: 346 449
Fragments arranged by size:
1607 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 323 335 344 637 1505 323
Size: 12 9 293 868 3401
Fragments arranged by size:
3401 868 293 12 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2533 2714 2877 2533
Size: 181 163 4239
Fragments arranged by size:
4239 181 163
BtsI GCAGTG_nn'
Cuts at: 1826 1826
Size: 4583
DraI TTT'AAA
Cuts at: 1582 1582
Size: 4583
DrdI GACnn_nn'nnGTC
Cuts at: 2289 3147 2289
Size: 858 3725
EaeI y'GGCC_r
Cuts at: 320 719 1004 1089 2168 2342 2733 2760 4105
Size: 399 285 85 1079 174 391 27 1345
Cuts at: 4105 320
Size: 798
Fragments arranged by size:
1345 1079 798 399 391 285 174 85
27
EagI C'GGCC_G
Cuts at: 320 719 1089 2168 320
Size: 399 370 1079 2735
Fragments arranged by size:
2735 1079 399 370
EarI CTCTTCn'nnn_
Cuts at: 180 228 2606 2816 180
Size: 48 2378 210 1947
Fragments arranged by size:
2378 1947 210 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 219 2791 3101 3247 4393 219
Size: 2572 310 146 1146 409
Fragments arranged by size:
2572 1146 409 310 146
EcoICRI GAG'CTC
Cuts at: 1595 1595
Size: 4583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 375 396 441 714 879 2408 2840 3587
Size: 105 21 45 273 165 1529 432 747
Cuts at: 3587 3855 270
Size: 268 998
Fragments arranged by size:
1529 998 747 432 273 268 165 105
45 21
EcoO109I rG'GnC_Cy
Cuts at: 281 495 1138 1167 1722 281
Size: 214 643 29 555 3142
Fragments arranged by size:
3142 643 555 214 29
EcoRI G'AATT_C
Cuts at: 1587 1587
Size: 4583
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4583
FspI TGC'GCA
Cuts at: 1387 2364 1387
Size: 977 3606
HaeII r_GCGC'y
Cuts at: 398 422 728 908 1034 1048 1296 1353 1380
Size: 24 306 180 126 14 248 57 27
Cuts at: 1380 1505 2017 2265 3287 4266 398
Size: 125 512 248 1022 979 715
Fragments arranged by size:
1022 979 715 512 306 248 248 180
126 125 57 27 24 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2425 2455 2457 2487 2425
Size: 30 2 30 4521
Fragments arranged by size:
4521 30 30 2
HincII GTy'rAC
Cuts at: 1622 1622
Size: 4583
HindIII A'AGCT_T
Cuts at: 264 264
Size: 4583
KasI G'GCGC_C
Cuts at: 724 904 1044 2013 2261 724
Size: 180 140 969 248 3046
Fragments arranged by size:
3046 969 248 180 140
KpnI G_GTAC'C
Cuts at: 1603 1603
Size: 4583
MluI A'CGCG_T
Cuts at: 4016 4016
Size: 4583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 383 1331 3254 3438 383
Size: 948 1923 184 1528
Fragments arranged by size:
1923 1528 948 184
MscI TGG'CCA
Cuts at: 2344 2344
Size: 4583
MslI CAynn'nnrTG
Cuts at: 610 2572 2699 610
Size: 1962 127 2494
Fragments arranged by size:
2494 1962 127
MspA1I CmG'CkG
Cuts at: 236 325 337 346 371 518 639 734 902
Size: 89 12 9 25 147 121 95 168
Cuts at: 902 1002 1050 1178 1320 1463 1507 1694 2008
Size: 100 48 128 142 143 44 187 314
Cuts at: 2008 2368 3381 3626 236
Size: 360 1013 245 1193
Fragments arranged by size:
1193 1013 360 314 245 187 168 147
143 142 128 121 100 95 89 48
44 25 12 9
NaeI GCC'GGC
Cuts at: 723 753 1008 2764 723
Size: 30 255 1756 2542
Fragments arranged by size:
2542 1756 255 30
NarI GG'CG_CC
Cuts at: 725 905 1045 2014 2262 725
Size: 180 140 969 248 3046
Fragments arranged by size:
3046 969 248 180 140
NgoMIV G'CCGG_C
Cuts at: 721 751 1006 2762 721
Size: 30 255 1756 2542
Fragments arranged by size:
2542 1756 255 30
NotI GC'GGCC_GC
Cuts at: 320 320
Size: 4583
NspI r_CATG'y
Cuts at: 1636 2136 3043 1636
Size: 500 907 3176
Fragments arranged by size:
3176 907 500
PciI A'CATG_T
Cuts at: 3039 3039
Size: 4583
PflMI CCAn_nnn'nTGG
Cuts at: 737 737
Size: 4583
PfoI T'CCnGG_A
Cuts at: 286 286
Size: 4583
PmeI GTTT'AAAC
Cuts at: 1582 1582
Size: 4583
PmlI CAC'GTG
Cuts at: 1410 1410
Size: 4583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 283 315 3722 3754 283
Size: 32 3407 32 1112
Fragments arranged by size:
3407 1112 32 32
PspOMI G'GGCC_C
Cuts at: 1137 1137
Size: 4583
PstI C_TGCA'G
Cuts at: 626 1319 1544 1630 2315 626
Size: 693 225 86 685 2894
Fragments arranged by size:
2894 693 685 225 86
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4583
PvuII CAG'CTG
Cuts at: 236 371 734 1178 2008 2368 236
Size: 135 363 444 830 360 2451
Fragments arranged by size:
2451 830 444 363 360 135
RsrII CG'GwC_CG
Cuts at: 2778 2778
Size: 4583
SacI G_AGCT'C
Cuts at: 1597 1597
Size: 4583
SacII CC_GC'GG
Cuts at: 326 338 347 640 1508 326
Size: 12 9 293 868 3401
Fragments arranged by size:
3401 868 293 12 9
SalI G'TCGA_C
Cuts at: 1620 1620
Size: 4583
SapI GCTCTTCn'nnn_
Cuts at: 180 2606 2816 180
Size: 2426 210 1947
Fragments arranged by size:
2426 1947 210
SbfI CC_TGCA'GG
Cuts at: 1630 1630
Size: 4583
ScaI AGT'ACT
Cuts at: 4100 4100
Size: 4583
SfcI C'TryA_G
Cuts at: 33 622 1315 1540 1626 2311 3304 3495 33
Size: 589 693 225 86 685 993 191 1121
Fragments arranged by size:
1121 993 693 685 589 225 191 86
SfoI GGC'GCC
Cuts at: 726 906 1046 2015 2263 726
Size: 180 140 969 248 3046
Fragments arranged by size:
3046 969 248 180 140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
SgrAI Cr'CCGG_yG
Cuts at: 1339 1339
Size: 4583
SmaI CCC'GGG
Cuts at: 689 1190 1557 1605 689
Size: 501 367 48 3667
Fragments arranged by size:
3667 501 367 48
SmlI C'TyrA_G
Cuts at: 261 1225 1738 2653 3145 3407 261
Size: 964 513 915 492 262 1437
Fragments arranged by size:
1437 964 915 513 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4583
SphI G_CATG'C
Cuts at: 1636 2136 1636
Size: 500 4083
SrfI GCCC'GGGC
Cuts at: 1557 1557
Size: 4583
StuI AGG'CCT
Cuts at: 878 878
Size: 4583
StyI C'CwwG_G
Cuts at: 596 1717 2071 596
Size: 1121 354 3108
Fragments arranged by size:
3108 1121 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 685 2166 2299 2758 2967 685
Size: 1481 133 459 209 2301
Fragments arranged by size:
2301 1481 459 209 133
TatI w'GTAC_w
Cuts at: 4098 4098
Size: 4583
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 4551
Tth111I GACn'n_nGTC
Cuts at: 2380 2380
Size: 4583
XbaI T'CTAG_A
Cuts at: 1614 1614
Size: 4583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2078 2078
Size: 4583
XmaI C'CCGG_G
Cuts at: 687 1188 1555 1603 687
Size: 501 367 48 3667
Fragments arranged by size:
3667 501 367 48
XmnI GAAnn'nnTTC
Cuts at: 1351 1351
Size: 4583
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PciI PflMI PfoI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BglI BmtI BsaXI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NruI NsiI
PacI PasI PpuMI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SnaBI SspI SwaI XhoI ZraI