Restriction map for
pF1KB9864
(Circular) (Six-base) MAPSORT of: pF1KB9864.seq Check: 9497 from: 1 to: 4379
pF1KB9864 4379 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1417 1417
Size: 4379
Acc65I G'GTAC_C
Cuts at: 1395 1395
Size: 4379
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2204 2636 3383 3651 2204
Size: 432 747 268 2932
Fragments arranged by size:
2932 747 432 268
AfeI AGC'GCT
Cuts at: 301 301
Size: 4379
AflIII A'CryG_T
Cuts at: 1334 1731 2835 3812 1334
Size: 397 1104 977 1901
Fragments arranged by size:
1901 1104 977 397
AgeI A'CCGG_T
Cuts at: 2767 2767
Size: 4379
AlwNI CAG_nnn'CTG
Cuts at: 512 893 1684 3251 512
Size: 381 791 1567 1640
Fragments arranged by size:
1640 1567 791 381
ApaLI G'TGCA_C
Cuts at: 991 3149 991
Size: 2158 2221
ApoI r'AATT_y
Cuts at: 1383 3732 3988 1383
Size: 2349 256 1774
Fragments arranged by size:
2349 1774 256
AscI GG'CGCG_CC
Cuts at: 561 561
Size: 4379
AseI AT'TA_AT
Cuts at: 20 20
Size: 4379
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 1121 1399 1121
Size: 278 4101
BamHI G'GATC_C
Cuts at: 1404 1404
Size: 4379
BanI G'GyrC_C
Cuts at: 967 1162 1252 1395 1809 2057 2092 967
Size: 195 90 143 414 248 35 3254
Fragments arranged by size:
3254 414 248 195 143 90 35
BanII G_rGCy'C
Cuts at: 1393 2423 1393
Size: 1030 3349
BbeI G_GCGC'C
Cuts at: 971 1256 1813 2061 971
Size: 285 557 248 3289
Fragments arranged by size:
3289 557 285 248
BbsI GAAGACnn'nnnn_
Cuts at: 650 817 1138 1147 650
Size: 167 321 9 3882
Fragments arranged by size:
3882 321 167 9
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3038 4379 2277
Size: 761 1341 2277
Fragments arranged by size:
2277 1341 761
BclI T'GATC_A
Cuts at: 218 1899 218
Size: 1681 2698
BglII A'GATC_T
Cuts at: 1894 3931 1894
Size: 2037 2342
BlpI GC'TnA_GC
Cuts at: 118 1491 118
Size: 1373 3006
Bme1580I G_kGCm'C
Cuts at: 241 995 1059 1165 2004 2097 3153 241
Size: 754 64 106 839 93 1056 1467
Fragments arranged by size:
1467 1056 839 754 106 93 64
BmgBI CAC'GTC
Cuts at: 198 456 198
Size: 258 4121
BmrI ACTGGGnnnn_n'
Cuts at: 427 522 1591 1672 2007 4151 427
Size: 95 1069 81 335 2144 655
Fragments arranged by size:
2144 1069 655 335 95 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 915 997 915
Size: 82 4297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 2434 2926 3224 1555
Size: 879 492 298 2710
Fragments arranged by size:
2710 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 160 4083 160
Size: 3923 456
BsaAI yAC'GTr
Cuts at: 540 1732 540
Size: 1192 3187
BsaBI GATnn'nnATC
Cuts at: 6 1893 3930 6
Size: 1887 2037 455
Fragments arranged by size:
2037 1887 455
BsaHI Gr'CG_yC
Cuts at: 553 968 1253 1810 2058 2760 4256 553
Size: 415 285 557 248 702 1496 676
Fragments arranged by size:
1496 702 676 557 415 285 248
BsaWI w'CCGG_w
Cuts at: 814 1568 2089 2767 3041 3188 3599 814
Size: 754 521 678 274 147 411 1594
Fragments arranged by size:
1594 754 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 234 261 624 900 919 1030 1146 1269 1804
Size: 27 363 276 19 111 116 123 535
Cuts at: 1804 2562 3139 3635 234
Size: 758 577 496 978
Fragments arranged by size:
978 758 577 535 496 363 276 123
116 111 27 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 450 450
Size: 4379
BsiEI CG_ry'CG
Cuts at: 104 1921 1967 3175 104
Size: 1817 46 1208 1308
Fragments arranged by size:
1817 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 119 404 930 995 1019 1393 2171 2361 3153
Size: 285 526 65 24 374 778 190 792
Cuts at: 3153 119
Size: 1345
Fragments arranged by size:
1345 792 778 526 374 285 190 65
24
Bsp1286I G_dGCh'C
Cuts at: 119 241 404 930 995 1019 1059 1165 1393
Size: 122 163 526 65 24 40 106 228
Cuts at: 1393 2004 2097 2171 2361 2423 3153 119
Size: 611 93 74 190 62 730 1345
Fragments arranged by size:
1345 730 611 526 228 190 163 122
106 93 74 65 62 40 24
BspEI T'CCGG_A
Cuts at: 3599 3599
Size: 4379
BspHI T'CATG_A
Cuts at: 3555 3555
Size: 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 439 1429 1643 1945 2326 439
Size: 990 214 302 381 2492
Fragments arranged by size:
2492 990 381 302 214
BsrBI CCG'CTC
Cuts at: 50 650 820 2671 2725 4209 4374 50
Size: 600 170 1851 54 1484 165 55
Fragments arranged by size:
1851 1484 600 170 165 55 54
BsrDI GCAATG_nn'
Cuts at: 273 2291 273
Size: 2018 2361
BsrFI r'CCGG_y
Cuts at: 153 169 2377 2558 2767 153
Size: 16 2208 181 209 1765
Fragments arranged by size:
2208 1765 209 181 16
BssHII G'CGCG_C
Cuts at: 561 772 561
Size: 211 4168
BssSI C'ACGA_G
Cuts at: 2650 3008 2650
Size: 358 4021
BstAPI GCAn_nnn'nTGC
Cuts at: 272 1756 272
Size: 1484 2895
BstBI TT'CG_AA
Cuts at: 2740 2740
Size: 4379
BstEII G'GTnAC_C
Cuts at: 345 426 345
Size: 81 4298
BstXI CCAn_nnnn'nTGG
Cuts at: 294 501 1248 294
Size: 207 747 3425
Fragments arranged by size:
3425 747 207
BstYI r'GATC_y
Cuts at: 1 1404 1871 1894 2229 2475 3487 3573 3585
Size: 1403 467 23 335 246 1012 86 12
Cuts at: 3585 3931 1
Size: 346 449
Fragments arranged by size:
1403 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 380 831 107
Size: 273 451 3655
Fragments arranged by size:
3655 451 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 273 429 480 2329 2510 2673 273
Size: 156 51 1849 181 163 1979
Fragments arranged by size:
1979 1849 181 163 156 51
BtsI GCAGTG_nn'
Cuts at: 1622 1622
Size: 4379
DraI TTT'AAA
Cuts at: 1378 1378
Size: 4379
DraIII CAC_nnn'GTG
Cuts at: 999 999
Size: 4379
DrdI GACnn_nn'nnGTC
Cuts at: 202 2085 2943 202
Size: 1883 858 1638
Fragments arranged by size:
1883 1638 858
EaeI y'GGCC_r
Cuts at: 317 383 1964 2138 2529 2556 3901 317
Size: 66 1581 174 391 27 1345 795
Fragments arranged by size:
1581 1345 795 391 174 66 27
EagI C'GGCC_G
Cuts at: 1964 1964
Size: 4379
EarI CTCTTCn'nnn_
Cuts at: 856 2402 2612 856
Size: 1546 210 2623
Fragments arranged by size:
2623 1546 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2587 2897 3043 4189 2587
Size: 310 146 1146 2777
Fragments arranged by size:
2777 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1391 1391
Size: 4379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 915 997 2204 2636 3383 3651 915
Size: 82 1207 432 747 268 1643
Fragments arranged by size:
1643 1207 747 432 268 82
EcoO109I rG'GnC_Cy
Cuts at: 1518 1518
Size: 4379
EcoRI G'AATT_C
Cuts at: 1383 1383
Size: 4379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4379
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4379
HaeII r_GCGC'y
Cuts at: 303 971 1256 1813 2061 3083 4062 303
Size: 668 285 557 248 1022 979 620
Fragments arranged by size:
1022 979 668 620 557 285 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 210 242 2221 2251 2253 2283 210
Size: 32 1979 30 2 30 2306
Fragments arranged by size:
2306 1979 32 30 30 2
HincII GTy'rAC
Cuts at: 1418 1418
Size: 4379
KasI G'GCGC_C
Cuts at: 967 1252 1809 2057 967
Size: 285 557 248 3289
Fragments arranged by size:
3289 557 285 248
KpnI G_GTAC'C
Cuts at: 1399 1399
Size: 4379
MfeI C'AATT_G
Cuts at: 1263 1263
Size: 4379
MluI A'CGCG_T
Cuts at: 1334 3812 1334
Size: 2478 1901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 218 1304 3050 3234 218
Size: 1086 1746 184 1363
Fragments arranged by size:
1746 1363 1086 184
MscI TGG'CCA
Cuts at: 319 2140 319
Size: 1821 2558
MslI CAynn'nnrTG
Cuts at: 112 1156 2368 2495 112
Size: 1044 1212 127 1996
Fragments arranged by size:
1996 1212 1044 127
MspA1I CmG'CkG
Cuts at: 568 729 1229 1490 1804 2164 3177 3422 568
Size: 161 500 261 314 360 1013 245 1525
Fragments arranged by size:
1525 1013 500 360 314 261 245 161
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4379
NarI GG'CG_CC
Cuts at: 968 1253 1810 2058 968
Size: 285 557 248 3289
Fragments arranged by size:
3289 557 285 248
NcoI C'CATG_G
Cuts at: 107 380 831 107
Size: 273 451 3655
Fragments arranged by size:
3655 451 273
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4379
NspI r_CATG'y
Cuts at: 668 1239 1432 1932 2839 668
Size: 571 193 500 907 2208
Fragments arranged by size:
2208 907 571 500 193
PciI A'CATG_T
Cuts at: 2835 2835
Size: 4379
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 4347
PstI C_TGCA'G
Cuts at: 311 1426 2111 311
Size: 1115 685 2579
Fragments arranged by size:
2579 1115 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 1229 1804 2164 1229
Size: 575 360 3444
Fragments arranged by size:
3444 575 360
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 1393 1393
Size: 4379
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4379
ScaI AGT'ACT
Cuts at: 3896 3896
Size: 4379
SfcI C'TryA_G
Cuts at: 33 307 1422 2107 3100 3291 33
Size: 274 1115 685 993 191 1121
Fragments arranged by size:
1121 1115 993 685 274 191
SfoI GGC'GCC
Cuts at: 969 1254 1811 2059 969
Size: 285 557 248 3289
Fragments arranged by size:
3289 557 285 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4379
SmlI C'TyrA_G
Cuts at: 1534 2449 2941 3203 1534
Size: 915 492 262 2710
Fragments arranged by size:
2710 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 668 1432 1932 668
Size: 764 500 3115
Fragments arranged by size:
3115 764 500
StyI C'CwwG_G
Cuts at: 107 293 380 831 1513 1867 107
Size: 186 87 451 682 354 2619
Fragments arranged by size:
2619 682 451 354 186 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1962 2095 2554 2763 1962
Size: 133 459 209 3578
Fragments arranged by size:
3578 459 209 133
TatI w'GTAC_w
Cuts at: 3894 3894
Size: 4379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4347
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4379
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1874 1874
Size: 4379
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4379
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI