Restriction map for
pF1KB9860
(Circular) (Six-base) MAPSORT of: pF1KB9860.seq Check: 5823 from: 1 to: 4331
pF1KB9860 4331 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1369 1369
Size: 4331
Acc65I G'GTAC_C
Cuts at: 1347 1347
Size: 4331
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1241 2156 2588 3335 3603 1241
Size: 915 432 747 268 1969
Fragments arranged by size:
1969 915 747 432 268
AfeI AGC'GCT
Cuts at: 532 532
Size: 4331
AflIII A'CryG_T
Cuts at: 1683 2787 3764 1683
Size: 1104 977 2250
Fragments arranged by size:
2250 1104 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AleI CACnn'nnGTG
Cuts at: 348 348
Size: 4331
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1285 1317 1285
Size: 32 4299
AlwNI CAG_nnn'CTG
Cuts at: 1636 3203 1636
Size: 1567 2764
ApaI G_GGCC'C
Cuts at: 293 380 293
Size: 87 4244
ApaLI G'TGCA_C
Cuts at: 3101 3101
Size: 4331
ApoI r'AATT_y
Cuts at: 409 1174 1335 3684 3940 409
Size: 765 161 2349 256 800
Fragments arranged by size:
2349 800 765 256 161
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 234 324 1351 234
Size: 90 1027 3214
Fragments arranged by size:
3214 1027 90
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 230 383 1347 1761 2009 2044 230
Size: 153 964 414 248 35 2517
Fragments arranged by size:
2517 964 414 248 153 35
BanII G_rGCy'C
Cuts at: 208 293 380 592 1345 2375 208
Size: 85 87 212 753 1030 2164
Fragments arranged by size:
2164 1030 753 212 87 85
BbeI G_GCGC'C
Cuts at: 234 387 1765 2013 234
Size: 153 1378 248 2552
Fragments arranged by size:
2552 1378 248 153
BbsI GAAGACnn'nnnn_
Cuts at: 140 140
Size: 4331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 415 449 415
Size: 34 4297
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BglI GCCn_nnn'nGGC
Cuts at: 545 545
Size: 4331
BglII A'GATC_T
Cuts at: 695 1846 3883 695
Size: 1151 2037 1143
Fragments arranged by size:
2037 1151 1143
BlpI GC'TnA_GC
Cuts at: 1443 1443
Size: 4331
Bme1580I G_kGCm'C
Cuts at: 189 293 380 1956 2049 3105 189
Size: 104 87 1576 93 1056 1415
Fragments arranged by size:
1576 1415 1056 104 93 87
BmrI ACTGGGnnnn_n'
Cuts at: 369 1543 1624 1959 4103 369
Size: 1174 81 335 2144 597
Fragments arranged by size:
2144 1174 597 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 380 501 1168 227
Size: 153 121 667 3390
Fragments arranged by size:
3390 667 153 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 1507 2386 2878 3176 222
Size: 1285 879 492 298 1377
Fragments arranged by size:
1377 1285 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4035 4035
Size: 4331
BsaAI yAC'GTr
Cuts at: 1684 1684
Size: 4331
BsaBI GATnn'nnATC
Cuts at: 6 1845 3882 6
Size: 1839 2037 455
Fragments arranged by size:
2037 1839 455
BsaHI Gr'CG_yC
Cuts at: 231 384 1762 2010 2712 4208 231
Size: 153 1378 248 702 1496 354
Fragments arranged by size:
1496 1378 702 354 248 153
BsaWI w'CCGG_w
Cuts at: 799 1520 2041 2719 2993 3140 3551 799
Size: 721 521 678 274 147 411 1579
Fragments arranged by size:
1579 721 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 181 211 181
Size: 30 4301
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 958 961 982 985 1027 1051 1072 1108 1117
Size: 3 21 3 42 24 21 36 9
Cuts at: 1117 1120 1132 1135 1153 1165 958
Size: 3 12 3 18 12 4124
Fragments arranged by size:
4124 42 36 24 21 21 18 12
12 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 182 368 503 533 786 1756 2514 3091 3587
Size: 186 135 30 253 970 758 577 496
Cuts at: 3587 182
Size: 926
Fragments arranged by size:
970 926 758 577 496 253 186 135
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1233 889
Size: 344 3987
BsiEI CG_ry'CG
Cuts at: 104 622 1873 1919 3127 104
Size: 518 1251 46 1208 1308
Fragments arranged by size:
1308 1251 1208 518 46
BsiHKAI G_wGCw'C
Cuts at: 208 243 355 610 1345 2123 2313 3105 208
Size: 35 112 255 735 778 190 792 1434
Fragments arranged by size:
1434 792 778 735 255 190 112 35
BsmI GAATG_Cn'
Cuts at: 886 886
Size: 4331
Bsp1286I G_dGCh'C
Cuts at: 189 208 243 293 355 380 592 610 1345
Size: 19 35 50 62 25 212 18 735
Cuts at: 1345 1956 2049 2123 2313 2375 3105 189
Size: 611 93 74 190 62 730 1415
Fragments arranged by size:
1415 735 730 611 212 190 93 74
62 62 50 35 25 19 18
BspEI T'CCGG_A
Cuts at: 799 3551 799
Size: 2752 1579
BspHI T'CATG_A
Cuts at: 686 3507 686
Size: 2821 1510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1381 1595 1897 2278 1381
Size: 214 302 381 3434
Fragments arranged by size:
3434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 485 773 2623 2677 4161 4326 50
Size: 435 288 1850 54 1484 165 55
Fragments arranged by size:
1850 1484 435 288 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2243 2243
Size: 4331
BsrFI r'CCGG_y
Cuts at: 621 2329 2510 2719 621
Size: 1708 181 209 2233
Fragments arranged by size:
2233 1708 209 181
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 1708 1708
Size: 4331
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 404 404
Size: 4331
BstYI r'GATC_y
Cuts at: 1 695 793 1356 1823 1846 2181 2427 3439
Size: 694 98 563 467 23 335 246 1012
Cuts at: 3439 3525 3537 3883 1
Size: 86 12 346 449
Fragments arranged by size:
1012 694 563 467 449 346 335 246
98 86 23 12
BtgI C'CryG_G
Cuts at: 549 583 616 764 549
Size: 34 33 148 4116
Fragments arranged by size:
4116 148 34 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 1574 1574
Size: 4331
DraI TTT'AAA
Cuts at: 1259 1330 1259
Size: 71 4260
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 619 1916 2090 2481 2508 3853 619
Size: 1297 174 391 27 1345 1097
Fragments arranged by size:
1345 1297 1097 391 174 27
EagI C'GGCC_G
Cuts at: 619 1916 619
Size: 1297 3034
EarI CTCTTCn'nnn_
Cuts at: 935 1016 1037 1061 1070 1109 1124 1130 2354
Size: 81 21 24 9 39 15 6 1224
Cuts at: 2354 2564 935
Size: 210 2702
Fragments arranged by size:
2702 1224 210 81 39 24 21 15
9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2539 2849 2995 4141 2539
Size: 310 146 1146 2729
Fragments arranged by size:
2729 1146 310 146
EcoICRI GAG'CTC
Cuts at: 206 1343 206
Size: 1137 3194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 380 501 1168 1241 2156 2588 3335 3603
Size: 153 121 667 73 915 432 747 268
Cuts at: 3603 227
Size: 955
Fragments arranged by size:
955 915 747 667 432 268 153 121
73
EcoO109I rG'GnC_Cy
Cuts at: 290 376 377 488 1143 1470 290
Size: 86 1 111 655 327 3151
Fragments arranged by size:
3151 655 327 111 86 1
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1268 2350 1268
Size: 1082 3249
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 234 387 534 664 748 1765 2013 3035 4014
Size: 153 147 130 84 1017 248 1022 979
Cuts at: 4014 234
Size: 551
Fragments arranged by size:
1022 1017 979 551 248 153 147 130
84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2173 2203 2205 2235 2173
Size: 30 2 30 4269
Fragments arranged by size:
4269 30 30 2
HincII GTy'rAC
Cuts at: 1370 1370
Size: 4331
KasI G'GCGC_C
Cuts at: 230 383 1761 2009 230
Size: 153 1378 248 2552
Fragments arranged by size:
2552 1378 248 153
KpnI G_GTAC'C
Cuts at: 1351 1351
Size: 4331
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 363 3002 3186 363
Size: 2639 184 1508
Fragments arranged by size:
2639 1508 184
MscI TGG'CCA
Cuts at: 2092 2092
Size: 4331
MslI CAynn'nnrTG
Cuts at: 348 2320 2447 348
Size: 1972 127 2232
Fragments arranged by size:
2232 1972 127
MspA1I CmG'CkG
Cuts at: 551 585 598 618 927 1442 1756 2116 3129
Size: 34 13 20 309 515 314 360 1013
Cuts at: 3129 3374 551
Size: 245 1508
Fragments arranged by size:
1508 1013 515 360 314 309 245 34
20 13
NaeI GCC'GGC
Cuts at: 2512 2512
Size: 4331
NarI GG'CG_CC
Cuts at: 231 384 1762 2010 231
Size: 153 1378 248 2552
Fragments arranged by size:
2552 1378 248 153
NgoMIV G'CCGG_C
Cuts at: 2510 2510
Size: 4331
NspI r_CATG'y
Cuts at: 657 1384 1884 2791 657
Size: 727 500 907 2197
Fragments arranged by size:
2197 907 727 500
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PfoI T'CCnGG_A
Cuts at: 396 490 1145 396
Size: 94 655 3582
Fragments arranged by size:
3582 655 94
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 4299
PpuMI rG'GwC_Cy
Cuts at: 488 1143 488
Size: 655 3676
PsiI TTA'TAA
Cuts at: 1243 1243
Size: 4331
PspOMI G'GGCC_C
Cuts at: 289 376 289
Size: 87 4244
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 682 714 682
Size: 32 4299
PstI C_TGCA'G
Cuts at: 848 1378 2063 848
Size: 530 685 3116
Fragments arranged by size:
3116 685 530
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 1756 2116 1756
Size: 360 3971
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 208 1345 208
Size: 1137 3194
SacII CC_GC'GG
Cuts at: 552 586 619 552
Size: 34 33 4264
Fragments arranged by size:
4264 34 33
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SfcI C'TryA_G
Cuts at: 33 844 1374 2059 3052 3243 33
Size: 811 530 685 993 191 1121
Fragments arranged by size:
1121 993 811 685 530 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 545 545
Size: 4331
SfoI GGC'GCC
Cuts at: 232 385 1763 2011 232
Size: 153 1378 248 2552
Fragments arranged by size:
2552 1378 248 153
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 236 326 1353 236
Size: 90 1027 3214
Fragments arranged by size:
3214 1027 90
SmlI C'TyrA_G
Cuts at: 201 1486 2401 2893 3155 201
Size: 1285 915 492 262 1377
Fragments arranged by size:
1377 1285 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 657 1384 1884 657
Size: 727 500 3104
Fragments arranged by size:
3104 727 500
SrfI GCCC'GGGC
Cuts at: 326 326
Size: 4331
StuI AGG'CCT
Cuts at: 781 781
Size: 4331
StyI C'CwwG_G
Cuts at: 295 1465 1819 295
Size: 1170 354 2807
Fragments arranged by size:
2807 1170 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 295 495 691 1914 2047 2506 2715 295
Size: 200 196 1223 133 459 209 1911
Fragments arranged by size:
1911 1223 459 209 200 196 133
TatI w'GTAC_w
Cuts at: 3846 3846
Size: 4331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 181 213 3848 3880 181
Size: 32 3635 32 632
Fragments arranged by size:
3635 632 32 32
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1826 1826
Size: 4331
XmaI C'CCGG_G
Cuts at: 234 324 1351 234
Size: 90 1027 3214
Fragments arranged by size:
3214 1027 90
XmnI GAAnn'nnTTC
Cuts at: 1175 1175
Size: 4331
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PspXI SanDI SexAI SgrAI SnaBI SspI SwaI
XhoI ZraI