Restriction map for
pF1KB9854
(Circular) (Six-base) MAPSORT of: pF1KB9854.seq Check: 2141 from: 1 to: 4280
pF1KB9854 4280 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 429 1318 429
Size: 889 3391
Acc65I G'GTAC_C
Cuts at: 1187 1296 1187
Size: 109 4171
AclI AA'CG_TT
Cuts at: 885 4120 885
Size: 3235 1045
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 838 2105 2537 3284 3552 812
Size: 26 1267 432 747 268 1540
Fragments arranged by size:
1540 1267 747 432 268 26
AflIII A'CryG_T
Cuts at: 1632 2736 3713 1632
Size: 1104 977 2199
Fragments arranged by size:
2199 1104 977
AgeI A'CCGG_T
Cuts at: 2668 2668
Size: 4280
AleI CACnn'nnGTG
Cuts at: 1253 1253
Size: 4280
AlwNI CAG_nnn'CTG
Cuts at: 1585 3152 1585
Size: 1567 2713
ApaLI G'TGCA_C
Cuts at: 3050 3050
Size: 4280
ApoI r'AATT_y
Cuts at: 201 394 573 586 616 1284 3633 3889 201
Size: 193 179 13 30 668 2349 256 592
Fragments arranged by size:
2349 668 592 256 193 179 30 13
AseI AT'TA_AT
Cuts at: 20 20
Size: 4280
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
AvaI C'yCGr_G
Cuts at: 1300 1300
Size: 4280
BamHI G'GATC_C
Cuts at: 648 1305 648
Size: 657 3623
BanI G'GyrC_C
Cuts at: 1187 1296 1710 1958 1993 1187
Size: 109 414 248 35 3474
Fragments arranged by size:
3474 414 248 109 35
BanII G_rGCy'C
Cuts at: 1294 2324 1294
Size: 1030 3250
BbeI G_GCGC'C
Cuts at: 1714 1962 1714
Size: 248 4032
BbsI GAAGACnn'nnnn_
Cuts at: 1068 1068
Size: 4280
BbvCI CC'TCA_GC
Cuts at: 1178 1178
Size: 4280
BciVI GTATCCnnnnn_n'
Cuts at: 2178 2939 4280 2178
Size: 761 1341 2178
Fragments arranged by size:
2178 1341 761
BclI T'GATC_A
Cuts at: 1079 1800 1079
Size: 721 3559
BglII A'GATC_T
Cuts at: 1795 3832 1795
Size: 2037 2243
BlpI GC'TnA_GC
Cuts at: 1392 1392
Size: 4280
Bme1580I G_kGCm'C
Cuts at: 1905 1998 3054 1905
Size: 93 1056 3131
Fragments arranged by size:
3131 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 575 1492 1573 1908 4052 575
Size: 917 81 335 2144 803
Fragments arranged by size:
2144 917 803 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4280
Bpu10I CC'TnA_GC
Cuts at: 1178 1208 1178
Size: 30 4250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 2335 2827 3125 1456
Size: 879 492 298 2611
Fragments arranged by size:
2611 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3984 3984
Size: 4280
BsaAI yAC'GTr
Cuts at: 498 1633 498
Size: 1135 3145
BsaBI GATnn'nnATC
Cuts at: 6 1794 3831 6
Size: 1788 2037 455
Fragments arranged by size:
2037 1788 455
BsaHI Gr'CG_yC
Cuts at: 1711 1959 2661 4157 1711
Size: 248 702 1496 1834
Fragments arranged by size:
1834 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1469 1990 2668 2942 3089 3500 1469
Size: 521 678 274 147 411 2249
Fragments arranged by size:
2249 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1055 1085 1055
Size: 30 4250
BseYI C'CCAG_C
Cuts at: 1705 2463 3040 3536 1705
Size: 758 577 496 2449
Fragments arranged by size:
2449 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 1231
Size: 4280
BsiEI CG_ry'CG
Cuts at: 104 1822 1868 3076 104
Size: 1718 46 1208 1308
Fragments arranged by size:
1718 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 898 1294 2072 2262 3054 898
Size: 396 778 190 792 2124
Fragments arranged by size:
2124 792 778 396 190
Bsp1286I G_dGCh'C
Cuts at: 898 1294 1905 1998 2072 2262 2324 3054 898
Size: 396 611 93 74 190 62 730 2124
Fragments arranged by size:
2124 730 611 396 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3500 3500
Size: 4280
BspHI T'CATG_A
Cuts at: 482 1046 3456 482
Size: 564 2410 1306
Fragments arranged by size:
2410 1306 564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1330 1544 1846 2227 1330
Size: 214 302 381 3383
Fragments arranged by size:
3383 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2572 2626 4110 4275 50
Size: 2522 54 1484 165 55
Fragments arranged by size:
2522 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 528 755 2192 528
Size: 227 1437 2616
Fragments arranged by size:
2616 1437 227
BsrFI r'CCGG_y
Cuts at: 2278 2459 2668 2278
Size: 181 209 3890
Fragments arranged by size:
3890 209 181
BssSI C'ACGA_G
Cuts at: 2551 2909 2551
Size: 358 3922
BstAPI GCAn_nnn'nTGC
Cuts at: 1040 1657 1040
Size: 617 3663
BstBI TT'CG_AA
Cuts at: 2641 2641
Size: 4280
BstYI r'GATC_y
Cuts at: 1 351 648 1305 1772 1795 2130 2376 3388
Size: 350 297 657 467 23 335 246 1012
Cuts at: 3388 3474 3486 3832 1
Size: 86 12 346 449
Fragments arranged by size:
1012 657 467 449 350 346 335 297
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2230 2411 2574 2230
Size: 181 163 3936
Fragments arranged by size:
3936 181 163
BtsI GCAGTG_nn'
Cuts at: 1243 1523 1243
Size: 280 4000
DraI TTT'AAA
Cuts at: 1279 1279
Size: 4280
DrdI GACnn_nn'nnGTC
Cuts at: 1986 2844 1986
Size: 858 3422
EaeI y'GGCC_r
Cuts at: 1865 2039 2430 2457 3802 1865
Size: 174 391 27 1345 2343
Fragments arranged by size:
2343 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1865 1865
Size: 4280
EarI CTCTTCn'nnn_
Cuts at: 788 2303 2513 788
Size: 1515 210 2555
Fragments arranged by size:
2555 1515 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2488 2798 2944 4090 2488
Size: 310 146 1146 2678
Fragments arranged by size:
2678 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1292 1292
Size: 4280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 838 2105 2537 3284 3552 812
Size: 26 1267 432 747 268 1540
Fragments arranged by size:
1540 1267 747 432 268 26
EcoO109I rG'GnC_Cy
Cuts at: 1419 1419
Size: 4280
EcoRI G'AATT_C
Cuts at: 1284 1284
Size: 4280
EcoRV GAT'ATC
Cuts at: 480 480
Size: 4280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 4280
FspI TGC'GCA
Cuts at: 2061 2061
Size: 4280
HaeII r_GCGC'y
Cuts at: 1714 1962 2984 3963 1714
Size: 248 1022 979 2031
Fragments arranged by size:
2031 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1055 1087 2122 2152 2154 2184 1055
Size: 32 1035 30 2 30 3151
Fragments arranged by size:
3151 1035 32 30 30 2
HincII GTy'rAC
Cuts at: 1319 1319
Size: 4280
KasI G'GCGC_C
Cuts at: 1710 1958 1710
Size: 248 4032
KpnI G_GTAC'C
Cuts at: 1191 1300 1191
Size: 109 4171
MluI A'CGCG_T
Cuts at: 3713 3713
Size: 4280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 517 2951 3135 517
Size: 2434 184 1662
Fragments arranged by size:
2434 1662 184
MscI TGG'CCA
Cuts at: 2041 2041
Size: 4280
MslI CAynn'nnrTG
Cuts at: 1087 1253 2269 2396 1087
Size: 166 1016 127 2971
Fragments arranged by size:
2971 1016 166 127
MspA1I CmG'CkG
Cuts at: 1177 1391 1705 2065 3078 3323 1177
Size: 214 314 360 1013 245 2134
Fragments arranged by size:
2134 1013 360 314 245 214
NaeI GCC'GGC
Cuts at: 2461 2461
Size: 4280
NarI GG'CG_CC
Cuts at: 1711 1959 1711
Size: 248 4032
NdeI CA'TA_TG
Cuts at: 775 775
Size: 4280
NgoMIV G'CCGG_C
Cuts at: 2459 2459
Size: 4280
NspI r_CATG'y
Cuts at: 729 1333 1833 2740 729
Size: 604 500 907 2269
Fragments arranged by size:
2269 907 604 500
PciI A'CATG_T
Cuts at: 2736 2736
Size: 4280
PflMI CCAn_nnn'nTGG
Cuts at: 190 190
Size: 4280
PfoI T'CCnGG_A
Cuts at: 740 740
Size: 4280
PmeI GTTT'AAAC
Cuts at: 1279 1279
Size: 4280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 4248
PsiI TTA'TAA
Cuts at: 151 151
Size: 4280
PstI C_TGCA'G
Cuts at: 829 1327 2012 829
Size: 498 685 3097
Fragments arranged by size:
3097 685 498
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4280
PvuII CAG'CTG
Cuts at: 1177 1705 2065 1177
Size: 528 360 3392
Fragments arranged by size:
3392 528 360
RsrII CG'GwC_CG
Cuts at: 2475 2475
Size: 4280
SacI G_AGCT'C
Cuts at: 1294 1294
Size: 4280
SalI G'TCGA_C
Cuts at: 1317 1317
Size: 4280
SapI GCTCTTCn'nnn_
Cuts at: 2303 2513 2303
Size: 210 4070
SbfI CC_TGCA'GG
Cuts at: 1327 1327
Size: 4280
ScaI AGT'ACT
Cuts at: 823 1103 3797 823
Size: 280 2694 1306
Fragments arranged by size:
2694 1306 280
SfcI C'TryA_G
Cuts at: 33 666 825 1323 2008 3001 3192 33
Size: 633 159 498 685 993 191 1121
Fragments arranged by size:
1121 993 685 633 498 191 159
SfoI GGC'GCC
Cuts at: 1712 1960 1712
Size: 248 4032
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
SmaI CCC'GGG
Cuts at: 1302 1302
Size: 4280
SmlI C'TyrA_G
Cuts at: 1435 2350 2842 3104 1435
Size: 915 492 262 2611
Fragments arranged by size:
2611 915 492 262
SnaBI TAC'GTA
Cuts at: 498 498
Size: 4280
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4280
SphI G_CATG'C
Cuts at: 1333 1833 1333
Size: 500 3780
SspI AAT'ATT
Cuts at: 1119 1119
Size: 4280
StyI C'CwwG_G
Cuts at: 1414 1768 1414
Size: 354 3926
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1256 1863 1996 2455 2664 1256
Size: 607 133 459 209 2872
Fragments arranged by size:
2872 607 459 209 133
TatI w'GTAC_w
Cuts at: 821 1101 3795 821
Size: 280 2694 1306
Fragments arranged by size:
2694 1306 280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3797 3829 3797
Size: 32 4248
Tth111I GACn'n_nGTC
Cuts at: 2077 2077
Size: 4280
XbaI T'CTAG_A
Cuts at: 420 898 1311 420
Size: 478 413 3389
Fragments arranged by size:
3389 478 413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1775 1775
Size: 4280
XmaI C'CCGG_G
Cuts at: 1300 1300
Size: 4280
XmnI GAAnn'nnTTC
Cuts at: 179 395 179
Size: 216 4064
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BpmI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
StuI SwaI XhoI ZraI