Restriction map for
pF1KB9844
(Circular) (Six-base) MAPSORT of: pF1KB9844.seq Check: 8681 from: 1 to: 4229
pF1KB9844 4229 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1267 1267
Size: 4229
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2486 3233 3501 2054
Size: 432 747 268 2782
Fragments arranged by size:
2782 747 432 268
AflIII A'CryG_T
Cuts at: 1581 2685 3662 1581
Size: 1104 977 2148
Fragments arranged by size:
2148 1104 977
AgeI A'CCGG_T
Cuts at: 2617 2617
Size: 4229
AleI CACnn'nnGTG
Cuts at: 959 959
Size: 4229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1117 1117
Size: 4229
AlwNI CAG_nnn'CTG
Cuts at: 1534 3101 1534
Size: 1567 2662
ApaI G_GGCC'C
Cuts at: 947 947
Size: 4229
ApaLI G'TGCA_C
Cuts at: 1044 2999 1044
Size: 1955 2274
ApoI r'AATT_y
Cuts at: 817 1233 3582 3838 817
Size: 416 2349 256 1208
Fragments arranged by size:
2349 1208 416 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 1249 1249
Size: 4229
BamHI G'GATC_C
Cuts at: 1254 1254
Size: 4229
BanI G'GyrC_C
Cuts at: 1245 1659 1907 1942 1245
Size: 414 248 35 3532
Fragments arranged by size:
3532 414 248 35
BanII G_rGCy'C
Cuts at: 173 194 947 994 1243 2273 173
Size: 21 753 47 249 1030 2129
Fragments arranged by size:
2129 1030 753 249 47 21
BbeI G_GCGC'C
Cuts at: 1663 1911 1663
Size: 248 3981
BbsI GAAGACnn'nnnn_
Cuts at: 449 1213 449
Size: 764 3465
BciVI GTATCCnnnnn_n'
Cuts at: 2127 2888 4229 2127
Size: 761 1341 2127
Fragments arranged by size:
2127 1341 761
BclI T'GATC_A
Cuts at: 1749 1749
Size: 4229
BglI GCCn_nnn'nGGC
Cuts at: 487 577 487
Size: 90 4139
BglII A'GATC_T
Cuts at: 1744 3781 1744
Size: 2037 2192
BlpI GC'TnA_GC
Cuts at: 1341 1341
Size: 4229
Bme1580I G_kGCm'C
Cuts at: 604 947 1048 1138 1854 1947 3003 604
Size: 343 101 90 716 93 1056 1830
Fragments arranged by size:
1830 1056 716 343 101 93 90
BmrI ACTGGGnnnn_n'
Cuts at: 1441 1522 1857 4001 1441
Size: 81 335 2144 1669
Fragments arranged by size:
2144 1669 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 576 664 166
Size: 410 88 3731
Fragments arranged by size:
3731 410 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1405 2284 2776 3074 1006
Size: 399 879 492 298 2161
Fragments arranged by size:
2161 879 492 399 298
BsaI GGTCTCn'nnnn_
Cuts at: 3933 3933
Size: 4229
BsaAI yAC'GTr
Cuts at: 957 1582 957
Size: 625 3604
BsaBI GATnn'nnATC
Cuts at: 6 1743 3780 6
Size: 1737 2037 455
Fragments arranged by size:
2037 1737 455
BsaHI Gr'CG_yC
Cuts at: 1660 1908 2610 4106 1660
Size: 248 702 1496 1783
Fragments arranged by size:
1783 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1418 1939 2617 2891 3038 3449 1418
Size: 521 678 274 147 411 2198
Fragments arranged by size:
2198 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 403 433 637 667 403
Size: 30 204 30 3965
Fragments arranged by size:
3965 204 30 30
BseYI C'CCAG_C
Cuts at: 529 541 1654 2412 2989 3485 529
Size: 12 1113 758 577 496 1273
Fragments arranged by size:
1273 1113 758 577 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 983 983
Size: 4229
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3025 104
Size: 1667 46 1208 1308
Fragments arranged by size:
1667 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 173 452 994 1048 1243 2021 2211 3003 173
Size: 279 542 54 195 778 190 792 1399
Fragments arranged by size:
1399 792 778 542 279 195 190 54
BsmBI CGTCTCn'nnnn_
Cuts at: 996 996
Size: 4229
Bsp1286I G_dGCh'C
Cuts at: 173 194 452 604 947 994 1048 1138 1243
Size: 21 258 152 343 47 54 90 105
Cuts at: 1243 1854 1947 2021 2211 2273 3003 173
Size: 611 93 74 190 62 730 1399
Fragments arranged by size:
1399 730 611 343 258 190 152 105
93 90 74 62 54 47 21
BspEI T'CCGG_A
Cuts at: 3449 3449
Size: 4229
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 621 1279 1493 1795 2176 621
Size: 658 214 302 381 2674
Fragments arranged by size:
2674 658 381 302 214
BsrBI CCG'CTC
Cuts at: 50 300 2521 2575 4059 4224 50
Size: 250 2221 54 1484 165 55
Fragments arranged by size:
2221 1484 250 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2141 2141
Size: 4229
BsrFI r'CCGG_y
Cuts at: 1024 1114 2227 2408 2617 1024
Size: 90 1113 181 209 2636
Fragments arranged by size:
2636 1113 209 181 90
BssSI C'ACGA_G
Cuts at: 603 2500 2858 603
Size: 1897 358 1974
Fragments arranged by size:
1974 1897 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1606 1606
Size: 4229
BstBI TT'CG_AA
Cuts at: 1052 2590 1052
Size: 1538 2691
BstEII G'GTnAC_C
Cuts at: 776 776
Size: 4229
BstYI r'GATC_y
Cuts at: 1 149 254 798 1254 1721 1744 2079 2325
Size: 148 105 544 456 467 23 335 246
Cuts at: 2325 3337 3423 3435 3781 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 544 467 456 449 346 335 246
148 105 86 23 12
BtgI C'CryG_G
Cuts at: 107 939 107
Size: 832 3397
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2179 2360 2523 2179
Size: 181 163 3885
Fragments arranged by size:
3885 181 163
BtsI GCAGTG_nn'
Cuts at: 707 1472 707
Size: 765 3464
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4229
DrdI GACnn_nn'nnGTC
Cuts at: 669 1935 2793 669
Size: 1266 858 2105
Fragments arranged by size:
2105 1266 858
EaeI y'GGCC_r
Cuts at: 264 1814 1988 2379 2406 3751 264
Size: 1550 174 391 27 1345 742
Fragments arranged by size:
1550 1345 742 391 174 27
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4229
EarI CTCTTCn'nnn_
Cuts at: 307 2252 2462 307
Size: 1945 210 2074
Fragments arranged by size:
2074 1945 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 266 2437 2747 2893 4039 266
Size: 2171 310 146 1146 456
Fragments arranged by size:
2171 1146 456 310 146
EcoICRI GAG'CTC
Cuts at: 171 992 1241 171
Size: 821 249 3159
Fragments arranged by size:
3159 821 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 576 664 2054 2486 3233 3501 166
Size: 410 88 1390 432 747 268 894
Fragments arranged by size:
1390 894 747 432 410 268 88
EcoO109I rG'GnC_Cy
Cuts at: 1368 1368
Size: 4229
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1000 2248 1000
Size: 1248 2981
FspI TGC'GCA
Cuts at: 1093 1105 2010 1093
Size: 12 905 3312
Fragments arranged by size:
3312 905 12
HaeII r_GCGC'y
Cuts at: 1663 1911 2933 3912 1663
Size: 248 1022 979 1980
Fragments arranged by size:
1980 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 912 944 2071 2101 2103 2133 912
Size: 32 1127 30 2 30 3008
Fragments arranged by size:
3008 1127 32 30 30 2
HincII GTy'rAC
Cuts at: 1172 1268 1172
Size: 96 4133
HindIII A'AGCT_T
Cuts at: 977 977
Size: 4229
KasI G'GCGC_C
Cuts at: 1659 1907 1659
Size: 248 3981
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MfeI C'AATT_G
Cuts at: 249 249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 238 2900 3084 140
Size: 98 2662 184 1285
Fragments arranged by size:
2662 1285 184 98
MscI TGG'CCA
Cuts at: 266 1990 266
Size: 1724 2505
MslI CAynn'nnrTG
Cuts at: 184 959 2218 2345 184
Size: 775 1259 127 2068
Fragments arranged by size:
2068 1259 775 127
MspA1I CmG'CkG
Cuts at: 545 810 1014 1340 1654 2014 3027 3272 545
Size: 265 204 326 314 360 1013 245 1502
Fragments arranged by size:
1502 1013 360 326 314 265 245 204
NaeI GCC'GGC
Cuts at: 2410 2410
Size: 4229
NarI GG'CG_CC
Cuts at: 1660 1908 1660
Size: 248 3981
NcoI C'CATG_G
Cuts at: 107 107
Size: 4229
NgoMIV G'CCGG_C
Cuts at: 2408 2408
Size: 4229
NspI r_CATG'y
Cuts at: 1204 1282 1782 2689 1204
Size: 78 500 907 2744
Fragments arranged by size:
2744 907 500 78
PciI A'CATG_T
Cuts at: 2685 2685
Size: 4229
PfoI T'CCnGG_A
Cuts at: 428 902 428
Size: 474 3755
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 4197
PspOMI G'GGCC_C
Cuts at: 943 943
Size: 4229
PstI C_TGCA'G
Cuts at: 1276 1961 1276
Size: 685 3544
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 810 1014 1654 2014 810
Size: 204 640 360 3025
Fragments arranged by size:
3025 640 360 204
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4229
SacI G_AGCT'C
Cuts at: 173 994 1243 173
Size: 821 249 3159
Fragments arranged by size:
3159 821 249
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 307 2252 2462 307
Size: 1945 210 2074
Fragments arranged by size:
2074 1945 210
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 745 3746 745
Size: 3001 1228
SfcI C'TryA_G
Cuts at: 33 1272 1957 2950 3141 33
Size: 1239 685 993 191 1121
Fragments arranged by size:
1239 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1661 1909 1661
Size: 248 3981
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SgrAI Cr'CCGG_yG
Cuts at: 1024 1114 1024
Size: 90 4139
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4229
SmlI C'TyrA_G
Cuts at: 985 1384 2299 2791 3053 985
Size: 399 915 492 262 2161
Fragments arranged by size:
2161 915 492 399 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 1282 1782 1282
Size: 500 3729
StyI C'CwwG_G
Cuts at: 107 693 1363 1717 107
Size: 586 670 354 2619
Fragments arranged by size:
2619 670 586 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 694 1812 1945 2404 2613 694
Size: 1118 133 459 209 2310
Fragments arranged by size:
2310 1118 459 209 133
TatI w'GTAC_w
Cuts at: 743 3744 743
Size: 3001 1228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 246 278 3746 3778 246
Size: 32 3468 32 697
Fragments arranged by size:
3468 697 32 32
Tth111I GACn'n_nGTC
Cuts at: 2026 2026
Size: 4229
XbaI T'CTAG_A
Cuts at: 1260 1260
Size: 4229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1724 1724
Size: 4229
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4229
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BseRI
BsiWI BsmI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI