Restriction map for
pF1KB9839
(Circular) (Six-base) MAPSORT of: pF1KB9839.seq Check: 3396 from: 1 to: 4178
pF1KB9839 4178 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1216 1216
Size: 4178
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2003 2435 3182 3450 2003
Size: 432 747 268 2731
Fragments arranged by size:
2731 747 432 268
AflIII A'CryG_T
Cuts at: 467 1530 2634 3611 467
Size: 1063 1104 977 1034
Fragments arranged by size:
1104 1063 1034 977
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 1066 1483 3050 1066
Size: 417 1567 2194
Fragments arranged by size:
2194 1567 417
ApaLI G'TGCA_C
Cuts at: 2948 2948
Size: 4178
ApoI r'AATT_y
Cuts at: 139 263 405 736 1182 3531 3787 139
Size: 124 142 331 446 2349 256 530
Fragments arranged by size:
2349 530 446 331 256 142 124
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 1198 1198
Size: 4178
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1063 1096 1063
Size: 33 4145
BamHI G'GATC_C
Cuts at: 1203 1203
Size: 4178
BanI G'GyrC_C
Cuts at: 713 1194 1608 1856 1891 713
Size: 481 414 248 35 3000
Fragments arranged by size:
3000 481 414 248 35
BanII G_rGCy'C
Cuts at: 917 1192 2222 917
Size: 275 1030 2873
Fragments arranged by size:
2873 1030 275
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 319 319
Size: 4178
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2837 4178 2076
Size: 761 1341 2076
Fragments arranged by size:
2076 1341 761
BclI T'GATC_A
Cuts at: 497 707 1698 497
Size: 210 991 2977
Fragments arranged by size:
2977 991 210
BglII A'GATC_T
Cuts at: 1693 3730 1693
Size: 2037 2141
BlpI GC'TnA_GC
Cuts at: 1290 1290
Size: 4178
Bme1580I G_kGCm'C
Cuts at: 1803 1896 2952 1803
Size: 93 1056 3029
Fragments arranged by size:
3029 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 231 453 1390 1471 1806 3950 231
Size: 222 937 81 335 2144 459
Fragments arranged by size:
2144 937 459 335 222 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 813 199
Size: 614 3564
Bpu10I CC'TnA_GC
Cuts at: 1126 1126
Size: 4178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1060 1154 1354 2233 2725 3023 544
Size: 516 94 200 879 492 298 1699
Fragments arranged by size:
1699 879 516 492 298 200 94
BsaI GGTCTCn'nnnn_
Cuts at: 231 3882 231
Size: 3651 527
BsaAI yAC'GTr
Cuts at: 1531 1531
Size: 4178
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1052 1367 1888 2566 2840 2987 3398 1052
Size: 315 521 678 274 147 411 1832
Fragments arranged by size:
1832 678 521 411 315 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 211 241 211
Size: 30 4148
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 208
Size: 4178
BseYI C'CCAG_C
Cuts at: 207 1603 2361 2938 3434 207
Size: 1396 758 577 496 951
Fragments arranged by size:
1396 951 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1720 1766 2974 104
Size: 1616 46 1208 1308
Fragments arranged by size:
1616 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 917 1192 1970 2160 2952 917
Size: 275 778 190 792 2143
Fragments arranged by size:
2143 792 778 275 190
BsmI GAATG_Cn'
Cuts at: 985 985
Size: 4178
Bsp1286I G_dGCh'C
Cuts at: 917 1192 1803 1896 1970 2160 2222 2952 917
Size: 275 611 93 74 190 62 730 2143
Fragments arranged by size:
2143 730 611 275 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 3354 3354
Size: 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1228 1442 1744 2125 1228
Size: 214 302 381 3281
Fragments arranged by size:
3281 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2470 2524 4008 4173 50
Size: 2420 54 1484 165 55
Fragments arranged by size:
2420 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 347 2090 347
Size: 1743 2435
BsrFI r'CCGG_y
Cuts at: 2176 2357 2566 2176
Size: 181 209 3788
Fragments arranged by size:
3788 209 181
BsrGI T'GTAC_A
Cuts at: 383 383
Size: 4178
BssSI C'ACGA_G
Cuts at: 2449 2807 2449
Size: 358 3820
BstAPI GCAn_nnn'nTGC
Cuts at: 1555 1555
Size: 4178
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstXI CCAn_nnnn'nTGG
Cuts at: 981 981
Size: 4178
BstYI r'GATC_y
Cuts at: 1 1203 1670 1693 2028 2274 3286 3372 3384
Size: 1202 467 23 335 246 1012 86 12
Cuts at: 3384 3730 1
Size: 346 449
Fragments arranged by size:
1202 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2128 2309 2472 2128
Size: 181 163 3834
Fragments arranged by size:
3834 181 163
BtsI GCAGTG_nn'
Cuts at: 452 1421 452
Size: 969 3209
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DraIII CAC_nnn'GTG
Cuts at: 329 948 1088 329
Size: 619 140 3419
Fragments arranged by size:
3419 619 140
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 398 1763 1937 2328 2355 3700 398
Size: 1365 174 391 27 1345 876
Fragments arranged by size:
1365 1345 876 391 174 27
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 760 2201 2411 760
Size: 1441 210 2527
Fragments arranged by size:
2527 1441 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 915 1190 915
Size: 275 3903
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 813 2003 2435 3182 3450 199
Size: 614 1190 432 747 268 927
Fragments arranged by size:
1190 927 747 614 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1317 1317
Size: 4178
EcoRI G'AATT_C
Cuts at: 263 1182 263
Size: 919 3259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 4178
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 1612 1860 2882 3861 1612
Size: 248 1022 979 1929
Fragments arranged by size:
1929 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 157 189 876 908 2020 2050 2052 2082 157
Size: 32 687 32 1112 30 2 30 2253
Fragments arranged by size:
2253 1112 687 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1217 1217
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3033 2849
Size: 184 3994
MscI TGG'CCA
Cuts at: 400 1939 400
Size: 1539 2639
MslI CAynn'nnrTG
Cuts at: 2167 2294 2167
Size: 127 4051
MspA1I CmG'CkG
Cuts at: 207 1100 1289 1603 1963 2976 3221 207
Size: 893 189 314 360 1013 245 1164
Fragments arranged by size:
1164 1013 893 360 314 245 189
NaeI GCC'GGC
Cuts at: 2359 2359
Size: 4178
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 107 107
Size: 4178
NgoMIV G'CCGG_C
Cuts at: 2357 2357
Size: 4178
NspI r_CATG'y
Cuts at: 471 1231 1731 2638 471
Size: 760 500 907 2011
Fragments arranged by size:
2011 907 760 500
PciI A'CATG_T
Cuts at: 467 2634 467
Size: 2167 2011
PflMI CCAn_nnn'nTGG
Cuts at: 329 329
Size: 4178
PfoI T'CCnGG_A
Cuts at: 267 632 1157 267
Size: 365 525 3288
Fragments arranged by size:
3288 525 365
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PstI C_TGCA'G
Cuts at: 1225 1910 1225
Size: 685 3493
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 207 1100 1603 1963 207
Size: 893 503 360 2422
Fragments arranged by size:
2422 893 503 360
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 917 1192 917
Size: 275 3903
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 2201 2411 2201
Size: 210 3968
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SfcI C'TryA_G
Cuts at: 33 1221 1906 2899 3090 33
Size: 1188 685 993 191 1121
Fragments arranged by size:
1188 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 559 1075 1169 1333 2248 2740 3002 559
Size: 516 94 164 915 492 262 1735
Fragments arranged by size:
1735 915 516 492 262 164 94
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
StyI C'CwwG_G
Cuts at: 107 1312 1666 107
Size: 1205 354 2619
Fragments arranged by size:
2619 1205 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 720 1761 1894 2353 2562 720
Size: 1041 133 459 209 2336
Fragments arranged by size:
2336 1041 459 209 133
TatI w'GTAC_w
Cuts at: 383 626 1079 3693 383
Size: 243 453 2614 868
Fragments arranged by size:
2614 868 453 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 211 243 3695 3727 211
Size: 32 3452 32 662
Fragments arranged by size:
3452 662 32 32
Tth111I GACn'n_nGTC
Cuts at: 1975 1975
Size: 4178
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 418 1673 114
Size: 304 1255 2619
Fragments arranged by size:
2619 1255 304
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI