Restriction map for
pF1KB9837
(Circular) (Six-base) MAPSORT of: pF1KB9837.seq Check: 8932 from: 1 to: 4169
pF1KB9837 4169 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 646 936 646
Size: 290 3879
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 485 1185 485
Size: 700 3469
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1994 2426 3173 3441 310
Size: 1684 432 747 268 1038
Fragments arranged by size:
1684 1038 747 432 268
AflIII A'CryG_T
Cuts at: 1521 2625 3602 1521
Size: 1104 977 2088
Fragments arranged by size:
2088 1104 977
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AleI CACnn'nnGTG
Cuts at: 565 565
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 1474 3041 1474
Size: 1567 2602
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 681 892 1173 3522 3778 681
Size: 211 281 2349 256 1072
Fragments arranged by size:
2349 1072 281 256 211
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 750 1102 1189 750
Size: 352 87 3730
Fragments arranged by size:
3730 352 87
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 485 1185 1599 1847 1882 485
Size: 700 414 248 35 2772
Fragments arranged by size:
2772 700 414 248 35
BanII G_rGCy'C
Cuts at: 164 369 428 855 1183 2213 164
Size: 205 59 427 328 1030 2120
Fragments arranged by size:
2120 1030 427 328 205 59
BbeI G_GCGC'C
Cuts at: 1603 1851 1603
Size: 248 3921
BbsI GAAGACnn'nnnn_
Cuts at: 619 1029 619
Size: 410 3759
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 557 1689 557
Size: 1132 3037
BglII A'GATC_T
Cuts at: 610 1684 3721 610
Size: 1074 2037 1058
Fragments arranged by size:
2037 1074 1058
BlpI GC'TnA_GC
Cuts at: 1281 1281
Size: 4169
Bme1580I G_kGCm'C
Cuts at: 1794 1887 2943 1794
Size: 93 1056 3020
Fragments arranged by size:
3020 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 378 1145 1381 1462 1797 3941 378
Size: 767 236 81 335 2144 606
Fragments arranged by size:
2144 767 606 335 236 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 4169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 2224 2716 3014 1345
Size: 879 492 298 2500
Fragments arranged by size:
2500 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3873 3873
Size: 4169
BsaAI yAC'GTr
Cuts at: 1522 1522
Size: 4169
BsaBI GATnn'nnATC
Cuts at: 6 703 1683 3720 6
Size: 697 980 2037 455
Fragments arranged by size:
2037 980 697 455
BsaHI Gr'CG_yC
Cuts at: 1600 1848 2550 4046 1600
Size: 248 702 1496 1723
Fragments arranged by size:
1723 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1358 1879 2557 2831 2978 3389 1358
Size: 521 678 274 147 411 2138
Fragments arranged by size:
2138 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1594 2352 2929 3425 1594
Size: 758 577 496 2338
Fragments arranged by size:
2338 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1183 1961 2151 2943 1183
Size: 778 190 792 2409
Fragments arranged by size:
2409 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 590 590
Size: 4169
Bsp1286I G_dGCh'C
Cuts at: 164 369 428 855 1183 1794 1887 1961 2151
Size: 205 59 427 328 611 93 74 190
Cuts at: 2151 2213 2943 164
Size: 62 730 1390
Fragments arranged by size:
1390 730 611 427 328 205 190 93
74 62 59
BspEI T'CCGG_A
Cuts at: 3389 3389
Size: 4169
BspHI T'CATG_A
Cuts at: 827 3345 827
Size: 2518 1651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 320 646 936 1219 1433 1735 2116 320
Size: 326 290 283 214 302 381 2373
Fragments arranged by size:
2373 381 326 302 290 283 214
BsrBI CCG'CTC
Cuts at: 50 213 2461 2515 3999 4164 50
Size: 163 2248 54 1484 165 55
Fragments arranged by size:
2248 1484 165 163 55 54
BsrDI GCAATG_nn'
Cuts at: 1052 2081 1052
Size: 1029 3140
BsrFI r'CCGG_y
Cuts at: 2167 2348 2557 2167
Size: 181 209 3779
Fragments arranged by size:
3779 209 181
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 1546 1546
Size: 4169
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstEII G'GTnAC_C
Cuts at: 531 531
Size: 4169
BstYI r'GATC_y
Cuts at: 1 610 1194 1661 1684 2019 2265 3277 3363
Size: 609 584 467 23 335 246 1012 86
Cuts at: 3363 3375 3721 1
Size: 12 346 449
Fragments arranged by size:
1012 609 584 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2119 2300 2463 2119
Size: 181 163 3825
Fragments arranged by size:
3825 181 163
BtsI GCAGTG_nn'
Cuts at: 972 1412 972
Size: 440 3729
ClaI AT'CG_AT
Cuts at: 479 479
Size: 4169
DraI TTT'AAA
Cuts at: 518 1168 518
Size: 650 3519
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 323 1754 1928 2319 2346 3691 323
Size: 1431 174 391 27 1345 801
Fragments arranged by size:
1431 1345 801 391 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 286 787 850 2192 2402 286
Size: 501 63 1342 210 2053
Fragments arranged by size:
2053 1342 501 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1181 1181
Size: 4169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1994 2426 3173 3441 310
Size: 1684 432 747 268 1038
Fragments arranged by size:
1684 1038 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1308 1308
Size: 4169
EcoRI G'AATT_C
Cuts at: 892 1173 892
Size: 281 3888
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 643 2188 643
Size: 1545 2624
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 1603 1851 2873 3852 1603
Size: 248 1022 979 1920
Fragments arranged by size:
1920 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 942 974 2011 2041 2043 2073 942
Size: 32 1037 30 2 30 3038
Fragments arranged by size:
3038 1037 32 30 30 2
HincII GTy'rAC
Cuts at: 412 1208 412
Size: 796 3373
KasI G'GCGC_C
Cuts at: 1599 1847 1599
Size: 248 3921
KpnI G_GTAC'C
Cuts at: 489 1189 489
Size: 700 3469
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 911 2840 3024 911
Size: 1929 184 2056
Fragments arranged by size:
2056 1929 184
MscI TGG'CCA
Cuts at: 325 1930 325
Size: 1605 2564
MslI CAynn'nnrTG
Cuts at: 565 826 2158 2285 565
Size: 261 1332 127 2449
Fragments arranged by size:
2449 1332 261 127
MspA1I CmG'CkG
Cuts at: 726 1280 1594 1954 2967 3212 726
Size: 554 314 360 1013 245 1683
Fragments arranged by size:
1683 1013 554 360 314 245
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 4169
NarI GG'CG_CC
Cuts at: 1600 1848 1600
Size: 248 3921
NcoI C'CATG_G
Cuts at: 107 107
Size: 4169
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 4169
NspI r_CATG'y
Cuts at: 255 1222 1722 2629 255
Size: 967 500 907 1795
Fragments arranged by size:
1795 967 907 500
PciI A'CATG_T
Cuts at: 2625 2625
Size: 4169
PfoI T'CCnGG_A
Cuts at: 887 887
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PstI C_TGCA'G
Cuts at: 643 1216 1901 643
Size: 573 685 2911
Fragments arranged by size:
2911 685 573
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 1594 1954 1594
Size: 360 3809
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 1183 1183
Size: 4169
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 286 2192 2402 286
Size: 1906 210 2053
Fragments arranged by size:
2053 1906 210
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 3686 3686
Size: 4169
SfcI C'TryA_G
Cuts at: 33 639 1212 1897 2890 3081 33
Size: 606 573 685 993 191 1121
Fragments arranged by size:
1121 993 685 606 573 191
SfoI GGC'GCC
Cuts at: 1601 1849 1601
Size: 248 3921
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 1191 1191
Size: 4169
SmlI C'TyrA_G
Cuts at: 1324 2239 2731 2993 1324
Size: 915 492 262 2500
Fragments arranged by size:
2500 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
StyI C'CwwG_G
Cuts at: 107 1303 1657 107
Size: 1196 354 2619
Fragments arranged by size:
2619 1196 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 555 1752 1885 2344 2553 555
Size: 1197 133 459 209 2171
Fragments arranged by size:
2171 1197 459 209 133
TatI w'GTAC_w
Cuts at: 3684 3684
Size: 4169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4137
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 391 895 1664 391
Size: 504 769 2896
Fragments arranged by size:
2896 769 504
XmaI C'CCGG_G
Cuts at: 1189 1189
Size: 4169
XmnI GAAnn'nnTTC
Cuts at: 626 682 883 626
Size: 56 201 3912
Fragments arranged by size:
3912 201 56
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsrGI
BssHII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI