Restriction map for
pF1KB9836
(Circular) (Six-base) MAPSORT of: pF1KB9836.seq Check: 400 from: 1 to: 4166
pF1KB9836 4166 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 684 684
Size: 4166
AccI GT'mk_AC
Cuts at: 1204 1204
Size: 4166
Acc65I G'GTAC_C
Cuts at: 1182 1182
Size: 4166
AclI AA'CG_TT
Cuts at: 4006 4006
Size: 4166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 1991 2423 3170 3438 550
Size: 1441 432 747 268 1278
Fragments arranged by size:
1441 1278 747 432 268
AfeI AGC'GCT
Cuts at: 167 167
Size: 4166
AflIII A'CryG_T
Cuts at: 1518 2622 3599 1518
Size: 1104 977 2085
Fragments arranged by size:
2085 1104 977
AgeI A'CCGG_T
Cuts at: 2554 2554
Size: 4166
AlwNI CAG_nnn'CTG
Cuts at: 942 1066 1471 3038 942
Size: 124 405 1567 2070
Fragments arranged by size:
2070 1567 405 124
ApaI G_GGCC'C
Cuts at: 537 537
Size: 4166
ApaLI G'TGCA_C
Cuts at: 2936 2936
Size: 4166
ApoI r'AATT_y
Cuts at: 430 646 1170 3519 3775 430
Size: 216 524 2349 256 821
Fragments arranged by size:
2349 821 524 256 216
AseI AT'TA_AT
Cuts at: 20 20
Size: 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
AvaI C'yCGr_G
Cuts at: 612 1186 612
Size: 574 3592
AvrII C'CTAG_G
Cuts at: 847 847
Size: 4166
BamHI G'GATC_C
Cuts at: 1191 1191
Size: 4166
BanI G'GyrC_C
Cuts at: 696 1182 1596 1844 1879 696
Size: 486 414 248 35 2983
Fragments arranged by size:
2983 486 414 248 35
BanII G_rGCy'C
Cuts at: 477 537 1180 2210 477
Size: 60 643 1030 2433
Fragments arranged by size:
2433 1030 643 60
BbeI G_GCGC'C
Cuts at: 1600 1848 1600
Size: 248 3918
BbsI GAAGACnn'nnnn_
Cuts at: 134 134
Size: 4166
BbvCI CC'TCA_GC
Cuts at: 1112 1112
Size: 4166
BciVI GTATCCnnnnn_n'
Cuts at: 2064 2825 4166 2064
Size: 761 1341 2064
Fragments arranged by size:
2064 1341 761
BclI T'GATC_A
Cuts at: 1686 1686
Size: 4166
BglI GCCn_nnn'nGGC
Cuts at: 740 740
Size: 4166
BglII A'GATC_T
Cuts at: 1681 3718 1681
Size: 2037 2129
BlpI GC'TnA_GC
Cuts at: 1278 1278
Size: 4166
Bme1580I G_kGCm'C
Cuts at: 537 1791 1884 2940 537
Size: 1254 93 1056 1763
Fragments arranged by size:
1763 1254 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1378 1459 1794 3938 1378
Size: 81 335 2144 1606
Fragments arranged by size:
2144 1606 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 491 2533 491
Size: 2042 2124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 611 943 1117 393
Size: 218 332 174 3442
Fragments arranged by size:
3442 332 218 174
Bpu10I CC'TnA_GC
Cuts at: 1112 1112
Size: 4166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 838 1342 2221 2713 3011 754
Size: 84 504 879 492 298 1909
Fragments arranged by size:
1909 879 504 492 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 3870 3870
Size: 4166
BsaAI yAC'GTr
Cuts at: 1519 1519
Size: 4166
BsaBI GATnn'nnATC
Cuts at: 6 1680 3717 6
Size: 1674 2037 455
Fragments arranged by size:
2037 1674 455
BsaHI Gr'CG_yC
Cuts at: 1597 1845 2547 4043 1597
Size: 248 702 1496 1720
Fragments arranged by size:
1720 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1355 1876 2554 2828 2975 3386 1355
Size: 521 678 274 147 411 2135
Fragments arranged by size:
2135 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 916 946 916
Size: 30 4136
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 835 968 1129 1132 1135 229
Size: 606 133 161 3 3 3260
Fragments arranged by size:
3260 606 161 133 3 3
BseYI C'CCAG_C
Cuts at: 744 1006 1059 1591 2349 2926 3422 744
Size: 262 53 532 758 577 496 1488
Fragments arranged by size:
1488 758 577 532 496 262 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 156
Size: 4166
BsiEI CG_ry'CG
Cuts at: 104 1708 1754 2962 104
Size: 1604 46 1208 1308
Fragments arranged by size:
1604 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 477 1180 1958 2148 2940 477
Size: 703 778 190 792 1703
Fragments arranged by size:
1703 792 778 703 190
Bsp1286I G_dGCh'C
Cuts at: 477 537 1180 1791 1884 1958 2148 2210 2940
Size: 60 643 611 93 74 190 62 730
Cuts at: 2940 477
Size: 1703
Fragments arranged by size:
1703 730 643 611 190 93 74 62
60
BspEI T'CCGG_A
Cuts at: 3386 3386
Size: 4166
BspHI T'CATG_A
Cuts at: 3342 3342
Size: 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 684 849 1216 1430 1732 2113 684
Size: 165 367 214 302 381 2737
Fragments arranged by size:
2737 381 367 302 214 165
BsrBI CCG'CTC
Cuts at: 50 2458 2512 3996 4161 50
Size: 2408 54 1484 165 55
Fragments arranged by size:
2408 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 586 1001 2078 586
Size: 415 1077 2674
Fragments arranged by size:
2674 1077 415
BsrFI r'CCGG_y
Cuts at: 178 2164 2345 2554 178
Size: 1986 181 209 1790
Fragments arranged by size:
1986 1790 209 181
BssSI C'ACGA_G
Cuts at: 2437 2795 2437
Size: 358 3808
BstAPI GCAn_nnn'nTGC
Cuts at: 1543 1543
Size: 4166
BstBI TT'CG_AA
Cuts at: 2527 2527
Size: 4166
BstYI r'GATC_y
Cuts at: 1 1191 1658 1681 2016 2262 3274 3360 3372
Size: 1190 467 23 335 246 1012 86 12
Cuts at: 3372 3718 1
Size: 346 449
Fragments arranged by size:
1190 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 344 344
Size: 4166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2116 2297 2460 2116
Size: 181 163 3822
Fragments arranged by size:
3822 181 163
BtsI GCAGTG_nn'
Cuts at: 204 1409 204
Size: 1205 2961
DraI TTT'AAA
Cuts at: 1165 1165
Size: 4166
DrdI GACnn_nn'nnGTC
Cuts at: 1872 2730 1872
Size: 858 3308
EaeI y'GGCC_r
Cuts at: 1751 1925 2316 2343 3688 1751
Size: 174 391 27 1345 2229
Fragments arranged by size:
2229 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1751 1751
Size: 4166
EarI CTCTTCn'nnn_
Cuts at: 569 706 2189 2399 569
Size: 137 1483 210 2336
Fragments arranged by size:
2336 1483 210 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2374 2684 2830 3976 2374
Size: 310 146 1146 2564
Fragments arranged by size:
2564 1146 310 146
EcoICRI GAG'CTC
Cuts at: 475 1178 475
Size: 703 3463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 550 611 943 1117 1991 2423 3170 3438
Size: 157 61 332 174 874 432 747 268
Cuts at: 3438 393
Size: 1121
Fragments arranged by size:
1121 874 747 432 332 268 174 157
61
EcoNI CCTnn'n_nnAGG
Cuts at: 1132 1132
Size: 4166
EcoO109I rG'GnC_Cy
Cuts at: 534 1125 1305 534
Size: 591 180 3395
Fragments arranged by size:
3395 591 180
EcoRI G'AATT_C
Cuts at: 1170 1170
Size: 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 4166
FspI TGC'GCA
Cuts at: 1947 1947
Size: 4166
HaeII r_GCGC'y
Cuts at: 169 1600 1848 2870 3849 169
Size: 1431 248 1022 979 486
Fragments arranged by size:
1431 1022 979 486 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 765 797 2008 2038 2040 2070 765
Size: 32 1211 30 2 30 2861
Fragments arranged by size:
2861 1211 32 30 30 2
HincII GTy'rAC
Cuts at: 1205 1205
Size: 4166
KasI G'GCGC_C
Cuts at: 1596 1844 1596
Size: 248 3918
KpnI G_GTAC'C
Cuts at: 1186 1186
Size: 4166
MfeI C'AATT_G
Cuts at: 959 959
Size: 4166
MluI A'CGCG_T
Cuts at: 3599 3599
Size: 4166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2837 3021 2837
Size: 184 3982
MscI TGG'CCA
Cuts at: 1927 1927
Size: 4166
MslI CAynn'nnrTG
Cuts at: 454 488 2155 2282 454
Size: 34 1667 127 2338
Fragments arranged by size:
2338 1667 127 34
MspA1I CmG'CkG
Cuts at: 1111 1277 1591 1951 2964 3209 1111
Size: 166 314 360 1013 245 2068
Fragments arranged by size:
2068 1013 360 314 245 166
NaeI GCC'GGC
Cuts at: 180 2347 180
Size: 2167 1999
NarI GG'CG_CC
Cuts at: 1597 1845 1597
Size: 248 3918
NgoMIV G'CCGG_C
Cuts at: 178 2345 178
Size: 2167 1999
NspI r_CATG'y
Cuts at: 1219 1719 2626 1219
Size: 500 907 2759
Fragments arranged by size:
2759 907 500
PciI A'CATG_T
Cuts at: 2622 2622
Size: 4166
PflMI CCAn_nnn'nTGG
Cuts at: 1054 1066 1054
Size: 12 4154
PfoI T'CCnGG_A
Cuts at: 521 521
Size: 4166
PmeI GTTT'AAAC
Cuts at: 1165 1165
Size: 4166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 4134
PspOMI G'GGCC_C
Cuts at: 533 533
Size: 4166
PstI C_TGCA'G
Cuts at: 199 227 812 1213 1898 199
Size: 28 585 401 685 2467
Fragments arranged by size:
2467 685 585 401 28
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4166
PvuII CAG'CTG
Cuts at: 1111 1591 1951 1111
Size: 480 360 3326
Fragments arranged by size:
3326 480 360
RsrII CG'GwC_CG
Cuts at: 2361 2361
Size: 4166
SacI G_AGCT'C
Cuts at: 477 1180 477
Size: 703 3463
SalI G'TCGA_C
Cuts at: 1203 1203
Size: 4166
SapI GCTCTTCn'nnn_
Cuts at: 569 2189 2399 569
Size: 1620 210 2336
Fragments arranged by size:
2336 1620 210
SbfI CC_TGCA'GG
Cuts at: 1213 1213
Size: 4166
ScaI AGT'ACT
Cuts at: 3683 3683
Size: 4166
SfcI C'TryA_G
Cuts at: 33 195 223 808 1209 1894 2887 3078 33
Size: 162 28 585 401 685 993 191 1121
Fragments arranged by size:
1121 993 685 585 401 191 162 28
SfoI GGC'GCC
Cuts at: 1598 1846 1598
Size: 248 3918
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
SmaI CCC'GGG
Cuts at: 1188 1188
Size: 4166
SmlI C'TyrA_G
Cuts at: 769 817 1321 2236 2728 2990 769
Size: 48 504 915 492 262 1945
Fragments arranged by size:
1945 915 504 492 262 48
SpeI A'CTAG_T
Cuts at: 67 325 67
Size: 258 3908
SphI G_CATG'C
Cuts at: 1219 1719 1219
Size: 500 3666
SspI AAT'ATT
Cuts at: 1142 1142
Size: 4166
StyI C'CwwG_G
Cuts at: 242 847 1300 1654 242
Size: 605 453 354 2754
Fragments arranged by size:
2754 605 453 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 448 1749 1882 2341 2550 448
Size: 1301 133 459 209 2064
Fragments arranged by size:
2064 1301 459 209 133
TatI w'GTAC_w
Cuts at: 251 912 3681 251
Size: 661 2769 736
Fragments arranged by size:
2769 736 661
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 4134
Tth111I GACn'n_nGTC
Cuts at: 997 1963 997
Size: 966 3200
XbaI T'CTAG_A
Cuts at: 1197 1197
Size: 4166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1003 1661 1003
Size: 658 3508
XmaI C'CCGG_G
Cuts at: 1186 1186
Size: 4166
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI