Restriction map for
pF1KB9830
(Circular) (Six-base) MAPSORT of: pF1KB9830.seq Check: 6309 from: 1 to: 4139
pF1KB9830 4139 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 119 1177 119
Size: 1058 3081
Acc65I G'GTAC_C
Cuts at: 1155 1155
Size: 4139
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1005 1066 1964 2396 3143 3411 1005
Size: 61 898 432 747 268 1733
Fragments arranged by size:
1733 898 747 432 268 61
AflII C'TTAA_G
Cuts at: 295 295
Size: 4139
AflIII A'CryG_T
Cuts at: 1491 2595 3572 1491
Size: 1104 977 2058
Fragments arranged by size:
2058 1104 977
AgeI A'CCGG_T
Cuts at: 758 2527 758
Size: 1769 2370
AlwNI CAG_nnn'CTG
Cuts at: 1444 3011 1444
Size: 1567 2572
ApaLI G'TGCA_C
Cuts at: 156 2909 156
Size: 2753 1386
ApoI r'AATT_y
Cuts at: 347 875 1026 1143 3492 3748 347
Size: 528 151 117 2349 256 738
Fragments arranged by size:
2349 738 528 256 151 117
AseI AT'TA_AT
Cuts at: 20 707 20
Size: 687 3452
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 899 1159 899
Size: 260 3879
BamHI G'GATC_C
Cuts at: 987 1164 987
Size: 177 3962
BanI G'GyrC_C
Cuts at: 1155 1569 1817 1852 1155
Size: 414 248 35 3442
Fragments arranged by size:
3442 414 248 35
BanII G_rGCy'C
Cuts at: 1153 2183 1153
Size: 1030 3109
BbeI G_GCGC'C
Cuts at: 1573 1821 1573
Size: 248 3891
BbsI GAAGACnn'nnnn_
Cuts at: 487 487
Size: 4139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4105
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2798 4139 2037
Size: 761 1341 2037
Fragments arranged by size:
2037 1341 761
BclI T'GATC_A
Cuts at: 1659 1659
Size: 4139
BfrBI ATG'CAT
Cuts at: 979 979
Size: 4139
BglII A'GATC_T
Cuts at: 1654 3691 1654
Size: 2037 2102
BlpI GC'TnA_GC
Cuts at: 1251 1251
Size: 4139
Bme1580I G_kGCm'C
Cuts at: 160 1054 1764 1857 2913 160
Size: 894 710 93 1056 1386
Fragments arranged by size:
1386 1056 894 710 93
BmrI ACTGGGnnnn_n'
Cuts at: 376 1351 1432 1767 3911 376
Size: 975 81 335 2144 604
Fragments arranged by size:
2144 975 604 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4139
Bpu10I CC'TnA_GC
Cuts at: 889 889
Size: 4139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 2194 2686 2984 1315
Size: 879 492 298 2470
Fragments arranged by size:
2470 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3843 3843
Size: 4139
BsaAI yAC'GTr
Cuts at: 768 1492 768
Size: 724 3415
BsaBI GATnn'nnATC
Cuts at: 6 463 1653 3690 6
Size: 457 1190 2037 455
Fragments arranged by size:
2037 1190 457 455
BsaHI Gr'CG_yC
Cuts at: 1570 1818 2520 4016 1570
Size: 248 702 1496 1693
Fragments arranged by size:
1693 1496 702 248
BsaWI w'CCGG_w
Cuts at: 266 758 1328 1849 2527 2801 2948 3359 266
Size: 492 570 521 678 274 147 411 1046
Fragments arranged by size:
1046 678 570 521 492 411 274 147
BseYI C'CCAG_C
Cuts at: 1564 2322 2899 3395 1564
Size: 758 577 496 2308
Fragments arranged by size:
2308 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 900 900
Size: 4139
BsiEI CG_ry'CG
Cuts at: 104 471 1681 1727 2935 104
Size: 367 1210 46 1208 1308
Fragments arranged by size:
1308 1210 1208 367 46
BsiHKAI G_wGCw'C
Cuts at: 160 955 1153 1931 2121 2913 160
Size: 795 198 778 190 792 1386
Fragments arranged by size:
1386 795 792 778 198 190
BsmI GAATG_Cn'
Cuts at: 556 556
Size: 4139
Bsp1286I G_dGCh'C
Cuts at: 160 955 1054 1153 1764 1857 1931 2121 2183
Size: 795 99 99 611 93 74 190 62
Cuts at: 2183 2913 160
Size: 730 1386
Fragments arranged by size:
1386 795 730 611 190 99 99 93
74 62
BspEI T'CCGG_A
Cuts at: 266 3359 266
Size: 3093 1046
BspHI T'CATG_A
Cuts at: 323 3315 323
Size: 2992 1147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1189 1403 1705 2086 1189
Size: 214 302 381 3242
Fragments arranged by size:
3242 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2431 2485 3969 4134 50
Size: 2381 54 1484 165 55
Fragments arranged by size:
2381 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 830 2051 830
Size: 1221 2918
BsrFI r'CCGG_y
Cuts at: 758 2137 2318 2527 758
Size: 1379 181 209 2370
Fragments arranged by size:
2370 1379 209 181
BssSI C'ACGA_G
Cuts at: 2410 2768 2410
Size: 358 3781
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 1516
Size: 4139
BstBI TT'CG_AA
Cuts at: 2500 2500
Size: 4139
BstEII G'GTnAC_C
Cuts at: 1108 1108
Size: 4139
BstYI r'GATC_y
Cuts at: 1 987 1164 1631 1654 1989 2235 3247 3333
Size: 986 177 467 23 335 246 1012 86
Cuts at: 3333 3345 3691 1
Size: 12 346 449
Fragments arranged by size:
1012 986 467 449 346 335 246 177
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 259 2089 2270 2433 259
Size: 1830 181 163 1965
Fragments arranged by size:
1965 1830 181 163
BtsI GCAGTG_nn'
Cuts at: 193 917 1382 193
Size: 724 465 2950
Fragments arranged by size:
2950 724 465
ClaI AT'CG_AT
Cuts at: 467 467
Size: 4139
DraI TTT'AAA
Cuts at: 498 1138 498
Size: 640 3499
DraIII CAC_nnn'GTG
Cuts at: 959 959
Size: 4139
DrdI GACnn_nn'nnGTC
Cuts at: 1845 2703 1845
Size: 858 3281
EaeI y'GGCC_r
Cuts at: 1724 1898 2289 2316 3661 1724
Size: 174 391 27 1345 2202
Fragments arranged by size:
2202 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1724 1724
Size: 4139
EarI CTCTTCn'nnn_
Cuts at: 193 1115 2162 2372 193
Size: 922 1047 210 1960
Fragments arranged by size:
1960 1047 922 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2347 2657 2803 3949 2347
Size: 310 146 1146 2537
Fragments arranged by size:
2537 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1151 1151
Size: 4139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1005 1066 1964 2396 3143 3411 1005
Size: 61 898 432 747 268 1733
Fragments arranged by size:
1733 898 747 432 268 61
EcoO109I rG'GnC_Cy
Cuts at: 183 1278 183
Size: 1095 3044
EcoRI G'AATT_C
Cuts at: 875 1143 875
Size: 268 3871
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 4139
FspI TGC'GCA
Cuts at: 1920 1920
Size: 4139
HaeII r_GCGC'y
Cuts at: 1573 1821 2843 3822 1573
Size: 248 1022 979 1890
Fragments arranged by size:
1890 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1981 2011 2013 2043 1981
Size: 30 2 30 4077
Fragments arranged by size:
4077 30 30 2
HincII GTy'rAC
Cuts at: 120 965 1178 120
Size: 845 213 3081
Fragments arranged by size:
3081 845 213
HpaI GTT'AAC
Cuts at: 965 965
Size: 4139
KasI G'GCGC_C
Cuts at: 1569 1817 1569
Size: 248 3891
KpnI G_GTAC'C
Cuts at: 1159 1159
Size: 4139
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 201 530 2810 2994 201
Size: 329 2280 184 1346
Fragments arranged by size:
2280 1346 329 184
MscI TGG'CCA
Cuts at: 1900 1900
Size: 4139
MslI CAynn'nnrTG
Cuts at: 2128 2255 2128
Size: 127 4012
MspA1I CmG'CkG
Cuts at: 1250 1564 1924 2937 3182 1250
Size: 314 360 1013 245 2207
Fragments arranged by size:
2207 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2320 2320
Size: 4139
NarI GG'CG_CC
Cuts at: 1570 1818 1570
Size: 248 3891
NgoMIV G'CCGG_C
Cuts at: 2318 2318
Size: 4139
NsiI A_TGCA'T
Cuts at: 981 981
Size: 4139
NspI r_CATG'y
Cuts at: 983 1192 1692 2599 983
Size: 209 500 907 2523
Fragments arranged by size:
2523 907 500 209
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PpuMI rG'GwC_Cy
Cuts at: 183 183
Size: 4139
PstI C_TGCA'G
Cuts at: 1186 1871 1186
Size: 685 3454
PvuI CG_AT'CG
Cuts at: 104 471 104
Size: 367 3772
PvuII CAG'CTG
Cuts at: 1564 1924 1564
Size: 360 3779
RsrII CG'GwC_CG
Cuts at: 2334 2334
Size: 4139
SacI G_AGCT'C
Cuts at: 1153 1153
Size: 4139
SalI G'TCGA_C
Cuts at: 118 1176 118
Size: 1058 3081
SapI GCTCTTCn'nnn_
Cuts at: 2162 2372 2162
Size: 210 3929
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SfcI C'TryA_G
Cuts at: 33 1182 1867 2860 3051 33
Size: 1149 685 993 191 1121
Fragments arranged by size:
1149 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1571 1819 1571
Size: 248 3891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 901 1161 901
Size: 260 3879
SmlI C'TyrA_G
Cuts at: 295 1294 2209 2701 2963 295
Size: 999 915 492 262 1471
Fragments arranged by size:
1471 999 915 492 262
SnaBI TAC'GTA
Cuts at: 768 768
Size: 4139
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 983 1192 1692 983
Size: 209 500 3430
Fragments arranged by size:
3430 500 209
StyI C'CwwG_G
Cuts at: 1273 1627 1273
Size: 354 3785
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 193 643 1722 1855 2314 2523 193
Size: 450 1079 133 459 209 1809
Fragments arranged by size:
1809 1079 459 450 209 133
TatI w'GTAC_w
Cuts at: 3654 3654
Size: 4139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4107
Tth111I GACn'n_nGTC
Cuts at: 1936 1936
Size: 4139
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 519 1634 519
Size: 1115 3024
XmaI C'CCGG_G
Cuts at: 899 1159 899
Size: 260 3879
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI BpmI
BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I BtgI CspCI EcoNI EcoRV FseI FspAI HindIII
MfeI NcoI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI XmnI ZraI