Restriction map for
pF1KB9828
(Circular) (Six-base) MAPSORT of: pF1KB9828.seq Check: 2175 from: 1 to: 4103
pF1KB9828 4103 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 963 963
Size: 4103
AccI GT'mk_AC
Cuts at: 1141 1141
Size: 4103
Acc65I G'GTAC_C
Cuts at: 332 1119 332
Size: 787 3316
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 923 1928 2360 3107 3375 923
Size: 1005 432 747 268 1651
Fragments arranged by size:
1651 1005 747 432 268
AfeI AGC'GCT
Cuts at: 427 427
Size: 4103
AflIII A'CryG_T
Cuts at: 824 1455 2559 3536 824
Size: 631 1104 977 1391
Fragments arranged by size:
1391 1104 977 631
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AleI CACnn'nnGTG
Cuts at: 535 535
Size: 4103
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1062 1094 1062
Size: 32 4071
AlwNI CAG_nnn'CTG
Cuts at: 978 1408 2975 978
Size: 430 1567 2106
Fragments arranged by size:
2106 1567 430
ApaI G_GGCC'C
Cuts at: 750 750
Size: 4103
ApaLI G'TGCA_C
Cuts at: 238 2873 238
Size: 2635 1468
ApoI r'AATT_y
Cuts at: 1107 3456 3712 1107
Size: 2349 256 1498
Fragments arranged by size:
2349 1498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 1123 1123
Size: 4103
BamHI G'GATC_C
Cuts at: 212 1128 212
Size: 916 3187
BanI G'GyrC_C
Cuts at: 332 758 1119 1533 1781 1816 332
Size: 426 361 414 248 35 2619
Fragments arranged by size:
2619 426 414 361 248 35
BanII G_rGCy'C
Cuts at: 130 750 994 1117 2147 130
Size: 620 244 123 1030 2086
Fragments arranged by size:
2086 1030 620 244 123
BbeI G_GCGC'C
Cuts at: 762 1537 1785 762
Size: 775 248 3080
Fragments arranged by size:
3080 775 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 402 436 90
Size: 34 278 34 3757
Fragments arranged by size:
3757 278 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 539 1623 539
Size: 1084 3019
BglI GCCn_nnn'nGGC
Cuts at: 196 645 196
Size: 449 3654
BglII A'GATC_T
Cuts at: 1618 3655 1618
Size: 2037 2066
BlpI GC'TnA_GC
Cuts at: 253 1215 253
Size: 962 3141
Bme1580I G_kGCm'C
Cuts at: 242 750 1728 1821 2877 242
Size: 508 978 93 1056 1468
Fragments arranged by size:
1468 1056 978 508 93
BmgBI CAC'GTC
Cuts at: 630 630
Size: 4103
BmrI ACTGGGnnnn_n'
Cuts at: 1315 1396 1731 3875 1315
Size: 81 335 2144 1543
Fragments arranged by size:
2144 1543 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 670
Size: 4103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1279 2158 2650 2948 1279
Size: 879 492 298 2434
Fragments arranged by size:
2434 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3807 3807
Size: 4103
BsaAI yAC'GTr
Cuts at: 398 1456 398
Size: 1058 3045
BsaBI GATnn'nnATC
Cuts at: 6 1617 3654 6
Size: 1611 2037 455
Fragments arranged by size:
2037 1611 455
BsaHI Gr'CG_yC
Cuts at: 759 1534 1782 2484 3980 759
Size: 775 248 702 1496 882
Fragments arranged by size:
1496 882 775 702 248
BsaWI w'CCGG_w
Cuts at: 1292 1813 2491 2765 2912 3323 1292
Size: 521 678 274 147 411 2072
Fragments arranged by size:
2072 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 448 478 448
Size: 30 4073
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 984 265
Size: 719 3384
BseYI C'CCAG_C
Cuts at: 224 731 1528 2286 2863 3359 224
Size: 507 797 758 577 496 968
Fragments arranged by size:
968 797 758 577 507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 472 513 215
Size: 257 41 3805
Fragments arranged by size:
3805 257 41
BsiEI CG_ry'CG
Cuts at: 104 1645 1691 2899 104
Size: 1541 46 1208 1308
Fragments arranged by size:
1541 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 130 242 668 1117 1895 2085 2877 130
Size: 112 426 449 778 190 792 1356
Fragments arranged by size:
1356 792 778 449 426 190 112
Bsp1286I G_dGCh'C
Cuts at: 130 242 668 750 994 1117 1728 1821 1895
Size: 112 426 82 244 123 611 93 74
Cuts at: 1895 2085 2147 2877 130
Size: 190 62 730 1356
Fragments arranged by size:
1356 730 611 426 244 190 123 112
93 82 74 62
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 3279 3279
Size: 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 344 561 604 963 1153 1367 1669 2050 344
Size: 217 43 359 190 214 302 381 2397
Fragments arranged by size:
2397 381 359 302 217 214 190 43
BsrBI CCG'CTC
Cuts at: 50 2395 2449 3933 4098 50
Size: 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 591 2015 591
Size: 1424 2679
BsrFI r'CCGG_y
Cuts at: 470 2101 2282 2491 470
Size: 1631 181 209 2082
Fragments arranged by size:
2082 1631 209 181
BssSI C'ACGA_G
Cuts at: 360 2374 2732 360
Size: 2014 358 1731
Fragments arranged by size:
2014 1731 358
BstAPI GCAn_nnn'nTGC
Cuts at: 389 978 1480 389
Size: 589 502 3012
Fragments arranged by size:
3012 589 502
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstXI CCAn_nnnn'nTGG
Cuts at: 186 186
Size: 4103
BstYI r'GATC_y
Cuts at: 1 212 1128 1595 1618 1953 2199 3211 3297
Size: 211 916 467 23 335 246 1012 86
Cuts at: 3297 3309 3655 1
Size: 12 346 449
Fragments arranged by size:
1012 916 467 449 346 335 246 211
86 23 12
BtgI C'CryG_G
Cuts at: 680 767 881 680
Size: 87 114 3902
Fragments arranged by size:
3902 114 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2053 2234 2397 2053
Size: 181 163 3759
Fragments arranged by size:
3759 181 163
BtsI GCAGTG_nn'
Cuts at: 525 1346 525
Size: 821 3282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 863 898 863
Size: 35 4068
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DraIII CAC_nnn'GTG
Cuts at: 837 837
Size: 4103
DrdI GACnn_nn'nnGTC
Cuts at: 1809 2667 1809
Size: 858 3245
EaeI y'GGCC_r
Cuts at: 266 1688 1862 2253 2280 3625 266
Size: 1422 174 391 27 1345 744
Fragments arranged by size:
1422 1345 744 391 174 27
EagI C'GGCC_G
Cuts at: 1688 1688
Size: 4103
EarI CTCTTCn'nnn_
Cuts at: 118 673 928 939 2126 2336 118
Size: 555 255 11 1187 210 1885
Fragments arranged by size:
1885 1187 555 255 210 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 205 225 2311 2621 2767 3913 205
Size: 20 2086 310 146 1146 395
Fragments arranged by size:
2086 1146 395 310 146 20
EcoICRI GAG'CTC
Cuts at: 128 1115 128
Size: 987 3116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 923 1928 2360 3107 3375 670
Size: 253 1005 432 747 268 1398
Fragments arranged by size:
1398 1005 747 432 268 253
EcoNI CCTnn'n_nnAGG
Cuts at: 340 340
Size: 4103
EcoO109I rG'GnC_Cy
Cuts at: 746 1242 746
Size: 496 3607
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 147 2122 147
Size: 1975 2128
FspI TGC'GCA
Cuts at: 949 1884 949
Size: 935 3168
HaeII r_GCGC'y
Cuts at: 429 762 1537 1785 2807 3786 429
Size: 333 775 248 1022 979 746
Fragments arranged by size:
1022 979 775 746 333 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 480 512 1945 1975 1977 2007 301
Size: 32 147 32 1433 30 2 30 2397
Fragments arranged by size:
2397 1433 147 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1142 1142
Size: 4103
KasI G'GCGC_C
Cuts at: 758 1533 1781 758
Size: 775 248 3080
Fragments arranged by size:
3080 775 248
KpnI G_GTAC'C
Cuts at: 336 1123 336
Size: 787 3316
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 605 2774 2958 605
Size: 2169 184 1750
Fragments arranged by size:
2169 1750 184
MscI TGG'CCA
Cuts at: 1864 1864
Size: 4103
MslI CAynn'nnrTG
Cuts at: 535 679 2092 2219 535
Size: 144 1413 127 2419
Fragments arranged by size:
2419 1413 144 127
MspA1I CmG'CkG
Cuts at: 329 1214 1528 1888 2901 3146 329
Size: 885 314 360 1013 245 1286
Fragments arranged by size:
1286 1013 885 360 314 245
NaeI GCC'GGC
Cuts at: 472 2284 472
Size: 1812 2291
NarI GG'CG_CC
Cuts at: 759 1534 1782 759
Size: 775 248 3080
Fragments arranged by size:
3080 775 248
NcoI C'CATG_G
Cuts at: 680 680
Size: 4103
NgoMIV G'CCGG_C
Cuts at: 470 2282 470
Size: 1812 2291
NspI r_CATG'y
Cuts at: 1156 1656 2563 1156
Size: 500 907 2696
Fragments arranged by size:
2696 907 500
PciI A'CATG_T
Cuts at: 2559 2559
Size: 4103
PflMI CCAn_nnn'nTGG
Cuts at: 837 837
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PmlI CAC'GTG
Cuts at: 398 398
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PspOMI G'GGCC_C
Cuts at: 746 746
Size: 4103
PstI C_TGCA'G
Cuts at: 394 974 1150 1835 394
Size: 580 176 685 2662
Fragments arranged by size:
2662 685 580 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 1528 1888 1528
Size: 360 3743
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 130 1117 130
Size: 987 3116
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SapI GCTCTTCn'nnn_
Cuts at: 118 673 928 2126 2336 118
Size: 555 255 1198 210 1885
Fragments arranged by size:
1885 1198 555 255 210
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SexAI A'CCwGG_T
Cuts at: 833 833
Size: 4103
SfcI C'TryA_G
Cuts at: 33 375 390 970 1091 1146 1831 2824 3015
Size: 342 15 580 121 55 685 993 191
Cuts at: 3015 33
Size: 1121
Fragments arranged by size:
1121 993 685 580 342 191 121 55
15
SfiI GGCCn_nnn'nGGCC
Cuts at: 196 196
Size: 4103
SfoI GGC'GCC
Cuts at: 760 1535 1783 760
Size: 775 248 3080
Fragments arranged by size:
3080 775 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 1125 1125
Size: 4103
SmlI C'TyrA_G
Cuts at: 1258 2173 2665 2927 1258
Size: 915 492 262 2434
Fragments arranged by size:
2434 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 1156 1656 1156
Size: 500 3603
StyI C'CwwG_G
Cuts at: 341 680 1237 1591 341
Size: 339 557 354 2853
Fragments arranged by size:
2853 557 354 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1686 1819 2278 2487 1686
Size: 133 459 209 3302
Fragments arranged by size:
3302 459 209 133
TatI w'GTAC_w
Cuts at: 455 3618 455
Size: 3163 940
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4071
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1598 1598
Size: 4103
XmaI C'CCGG_G
Cuts at: 1123 1123
Size: 4103
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PmlI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AscI AvrII BaeI BbsI
BbvCI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI