Restriction map for
pF1KB9826
(Circular) (Six-base) MAPSORT of: pF1KB9826.seq Check: 1315 from: 1 to: 4076
pF1KB9826 4076 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1114 1114
Size: 4076
Acc65I G'GTAC_C
Cuts at: 1092 1092
Size: 4076
AclI AA'CG_TT
Cuts at: 3916 3916
Size: 4076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1901 2333 3080 3348 862
Size: 1039 432 747 268 1590
Fragments arranged by size:
1590 1039 747 432 268
AfeI AGC'GCT
Cuts at: 617 617
Size: 4076
AflIII A'CryG_T
Cuts at: 1428 2532 3509 1428
Size: 1104 977 1995
Fragments arranged by size:
1995 1104 977
AgeI A'CCGG_T
Cuts at: 2464 2464
Size: 4076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 341 676 341
Size: 335 3741
AlwNI CAG_nnn'CTG
Cuts at: 1381 2948 1381
Size: 1567 2509
ApaI G_GGCC'C
Cuts at: 376 376
Size: 4076
ApaLI G'TGCA_C
Cuts at: 172 2846 172
Size: 2674 1402
ApoI r'AATT_y
Cuts at: 1080 3429 3685 1080
Size: 2349 256 1471
Fragments arranged by size:
2349 1471 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 354 551 625 899 1096 354
Size: 197 74 274 197 3334
Fragments arranged by size:
3334 274 197 197 74
BamHI G'GATC_C
Cuts at: 1101 1101
Size: 4076
BanI G'GyrC_C
Cuts at: 378 718 1092 1506 1754 1789 378
Size: 340 374 414 248 35 2665
Fragments arranged by size:
2665 414 374 340 248 35
BanII G_rGCy'C
Cuts at: 238 376 422 626 898 1017 1090 2120 238
Size: 138 46 204 272 119 73 1030 2194
Fragments arranged by size:
2194 1030 272 204 138 119 73 46
BbeI G_GCGC'C
Cuts at: 722 1510 1758 722
Size: 788 248 3040
Fragments arranged by size:
3040 788 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 297 331 297
Size: 34 4042
BciVI GTATCCnnnnn_n'
Cuts at: 1974 2735 4076 1974
Size: 761 1341 1974
Fragments arranged by size:
1974 1341 761
BclI T'GATC_A
Cuts at: 458 1596 458
Size: 1138 2938
BglI GCCn_nnn'nGGC
Cuts at: 349 349
Size: 4076
BglII A'GATC_T
Cuts at: 1591 3628 1591
Size: 2037 2039
BlpI GC'TnA_GC
Cuts at: 649 1188 649
Size: 539 3537
Bme1580I G_kGCm'C
Cuts at: 176 376 1701 1794 2850 176
Size: 200 1325 93 1056 1402
Fragments arranged by size:
1402 1325 1056 200 93
BmgBI CAC'GTC
Cuts at: 798 798
Size: 4076
BmrI ACTGGGnnnn_n'
Cuts at: 1288 1369 1704 3848 1288
Size: 81 335 2144 1516
Fragments arranged by size:
2144 1516 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 257 2443 257
Size: 2186 1890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1021 640
Size: 381 3695
Bpu10I CC'TnA_GC
Cuts at: 691 691
Size: 4076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 2131 2623 2921 1252
Size: 879 492 298 2407
Fragments arranged by size:
2407 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3780 3780
Size: 4076
BsaAI yAC'GTr
Cuts at: 432 705 864 1429 432
Size: 273 159 565 3079
Fragments arranged by size:
3079 565 273 159
BsaBI GATnn'nnATC
Cuts at: 6 460 928 1590 3627 6
Size: 454 468 662 2037 455
Fragments arranged by size:
2037 662 468 455 454
BsaHI Gr'CG_yC
Cuts at: 719 1507 1755 2457 3953 719
Size: 788 248 702 1496 842
Fragments arranged by size:
1496 842 788 702 248
BsaWI w'CCGG_w
Cuts at: 1265 1786 2464 2738 2885 3296 1265
Size: 521 678 274 147 411 2045
Fragments arranged by size:
2045 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 243 273 243
Size: 30 4046
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 230 346 643 832 1018 230
Size: 116 297 189 186 3288
Fragments arranged by size:
3288 297 189 186 116
BseYI C'CCAG_C
Cuts at: 365 1501 2259 2836 3332 365
Size: 1136 758 577 496 1109
Fragments arranged by size:
1136 1109 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 534 550 571 424
Size: 110 16 21 3929
Fragments arranged by size:
3929 110 21 16
BsiEI CG_ry'CG
Cuts at: 104 257 1618 1664 2872 104
Size: 153 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 153 46
BsiHKAI G_wGCw'C
Cuts at: 176 422 1017 1090 1868 2058 2850 176
Size: 246 595 73 778 190 792 1402
Fragments arranged by size:
1402 792 778 595 246 190 73
Bsp1286I G_dGCh'C
Cuts at: 176 238 376 422 626 898 1017 1090 1701
Size: 62 138 46 204 272 119 73 611
Cuts at: 1701 1794 1868 2058 2120 2850 176
Size: 93 74 190 62 730 1402
Fragments arranged by size:
1402 730 611 272 204 190 138 119
93 74 73 62 62 46
BspEI T'CCGG_A
Cuts at: 3296 3296
Size: 4076
BspHI T'CATG_A
Cuts at: 683 3252 683
Size: 2569 1507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1126 1340 1642 2023 1126
Size: 214 302 381 3179
Fragments arranged by size:
3179 381 302 214
BsrBI CCG'CTC
Cuts at: 50 259 2368 2422 3906 4071 50
Size: 209 2109 54 1484 165 55
Fragments arranged by size:
2109 1484 209 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1988 1988
Size: 4076
BsrFI r'CCGG_y
Cuts at: 2074 2255 2464 2074
Size: 181 209 3686
Fragments arranged by size:
3686 209 181
BssSI C'ACGA_G
Cuts at: 415 2347 2705 415
Size: 1932 358 1786
Fragments arranged by size:
1932 1786 358
BstAPI GCAn_nnn'nTGC
Cuts at: 402 1453 402
Size: 1051 3025
BstBI TT'CG_AA
Cuts at: 2437 2437
Size: 4076
BstYI r'GATC_y
Cuts at: 1 126 1101 1568 1591 1926 2172 3184 3270
Size: 125 975 467 23 335 246 1012 86
Cuts at: 3270 3282 3628 1
Size: 12 346 449
Fragments arranged by size:
1012 975 467 449 346 335 246 125
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 571 571
Size: 4076
BtgI C'CryG_G
Cuts at: 593 714 593
Size: 121 3955
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2026 2207 2370 2026
Size: 181 163 3732
Fragments arranged by size:
3732 181 163
BtsI GCAGTG_nn'
Cuts at: 1034 1062 1319 1034
Size: 28 257 3791
Fragments arranged by size:
3791 257 28
DraI TTT'AAA
Cuts at: 1075 1075
Size: 4076
DrdI GACnn_nn'nnGTC
Cuts at: 1782 2640 1782
Size: 858 3218
EaeI y'GGCC_r
Cuts at: 254 944 1661 1835 2226 2253 3598 254
Size: 690 717 174 391 27 1345 732
Fragments arranged by size:
1345 732 717 690 391 174 27
EagI C'GGCC_G
Cuts at: 254 1661 254
Size: 1407 2669
EarI CTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2284 2594 2740 3886 2284
Size: 310 146 1146 2474
Fragments arranged by size:
2474 1146 310 146
EcoICRI GAG'CTC
Cuts at: 420 1015 1088 420
Size: 595 73 3408
Fragments arranged by size:
3408 595 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 862 1021 1901 2333 3080 3348 640
Size: 222 159 880 432 747 268 1368
Fragments arranged by size:
1368 880 747 432 268 222 159
EcoNI CCTnn'n_nnAGG
Cuts at: 242 319 242
Size: 77 3999
EcoO109I rG'GnC_Cy
Cuts at: 156 285 312 351 1215 156
Size: 129 27 39 864 3017
Fragments arranged by size:
3017 864 129 39 27
EcoRI G'AATT_C
Cuts at: 1080 1080
Size: 4076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 4076
FspI TGC'GCA
Cuts at: 751 1857 751
Size: 1106 2970
HaeII r_GCGC'y
Cuts at: 619 649 722 734 1510 1758 2780 3759 619
Size: 30 73 12 776 248 1022 979 936
Fragments arranged by size:
1022 979 936 776 248 73 30 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 243 275 1918 1948 1950 1980 243
Size: 32 1643 30 2 30 2339
Fragments arranged by size:
2339 1643 32 30 30 2
HincII GTy'rAC
Cuts at: 1115 1115
Size: 4076
KasI G'GCGC_C
Cuts at: 718 1506 1754 718
Size: 788 248 3040
Fragments arranged by size:
3040 788 248
KpnI G_GTAC'C
Cuts at: 1096 1096
Size: 4076
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2747 2931 2747
Size: 184 3892
MscI TGG'CCA
Cuts at: 946 1837 946
Size: 891 3185
MslI CAynn'nnrTG
Cuts at: 664 703 922 2065 2192 664
Size: 39 219 1143 127 2548
Fragments arranged by size:
2548 1143 219 127 39
MspA1I CmG'CkG
Cuts at: 399 716 1187 1501 1861 2874 3119 399
Size: 317 471 314 360 1013 245 1356
Fragments arranged by size:
1356 1013 471 360 317 314 245
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 4076
NarI GG'CG_CC
Cuts at: 719 1507 1755 719
Size: 788 248 3040
Fragments arranged by size:
3040 788 248
NcoI C'CATG_G
Cuts at: 593 593
Size: 4076
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 4076
NspI r_CATG'y
Cuts at: 663 1129 1629 2536 663
Size: 466 500 907 2203
Fragments arranged by size:
2203 907 500 466
PasI CC'CwG_GG
Cuts at: 184 184
Size: 4076
PciI A'CATG_T
Cuts at: 2532 2532
Size: 4076
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 4044
PspOMI G'GGCC_C
Cuts at: 372 372
Size: 4076
PstI C_TGCA'G
Cuts at: 320 1123 1808 320
Size: 803 685 2588
Fragments arranged by size:
2588 803 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 399 1501 1861 399
Size: 1102 360 2614
Fragments arranged by size:
2614 1102 360
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 422 1017 1090 422
Size: 595 73 3408
Fragments arranged by size:
3408 595 73
SacII CC_GC'GG
Cuts at: 717 717
Size: 4076
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SapI GCTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
SbfI CC_TGCA'GG
Cuts at: 1123 1123
Size: 4076
ScaI AGT'ACT
Cuts at: 3593 3593
Size: 4076
SfcI C'TryA_G
Cuts at: 33 316 1119 1804 2797 2988 33
Size: 283 803 685 993 191 1121
Fragments arranged by size:
1121 993 803 685 283 191
SfoI GGC'GCC
Cuts at: 720 1508 1756 720
Size: 788 248 3040
Fragments arranged by size:
3040 788 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 553 901 1098 553
Size: 348 197 3531
Fragments arranged by size:
3531 348 197
SmlI C'TyrA_G
Cuts at: 1231 2146 2638 2900 1231
Size: 915 492 262 2407
Fragments arranged by size:
2407 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 663 1129 1629 663
Size: 466 500 3110
Fragments arranged by size:
3110 500 466
StuI AGG'CCT
Cuts at: 674 973 674
Size: 299 3777
StyI C'CwwG_G
Cuts at: 593 1210 1564 593
Size: 617 354 3105
Fragments arranged by size:
3105 617 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1659 1792 2251 2460 1659
Size: 133 459 209 3275
Fragments arranged by size:
3275 459 209 133
TatI w'GTAC_w
Cuts at: 3591 3591
Size: 4076
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4044
Tth111I GACn'n_nGTC
Cuts at: 1873 1873
Size: 4076
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1571 1571
Size: 4076
XmaI C'CCGG_G
Cuts at: 551 899 1096 551
Size: 348 197 3531
Fragments arranged by size:
3531 348 197
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI