Restriction map for
pF1KB9824
(Circular) (Six-base) MAPSORT of: pF1KB9824.seq Check: 9101 from: 1 to: 4058
pF1KB9824 4058 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1096 1096
Size: 4058
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4058
AclI AA'CG_TT
Cuts at: 3898 3898
Size: 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1883 2315 3062 3330 1883
Size: 432 747 268 2611
Fragments arranged by size:
2611 747 432 268
AflII C'TTAA_G
Cuts at: 595 595
Size: 4058
AflIII A'CryG_T
Cuts at: 222 1410 2514 3491 222
Size: 1188 1104 977 789
Fragments arranged by size:
1188 1104 977 789
AgeI A'CCGG_T
Cuts at: 2446 2446
Size: 4058
AlwNI CAG_nnn'CTG
Cuts at: 510 1363 2930 510
Size: 853 1567 1638
Fragments arranged by size:
1638 1567 853
ApaLI G'TGCA_C
Cuts at: 672 2828 672
Size: 2156 1902
ApoI r'AATT_y
Cuts at: 1062 3411 3667 1062
Size: 2349 256 1453
Fragments arranged by size:
2349 1453 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
AvaI C'yCGr_G
Cuts at: 1078 1078
Size: 4058
AvrII C'CTAG_G
Cuts at: 382 1030 382
Size: 648 3410
BamHI G'GATC_C
Cuts at: 1083 1083
Size: 4058
BanI G'GyrC_C
Cuts at: 1074 1488 1736 1771 1074
Size: 414 248 35 3361
Fragments arranged by size:
3361 414 248 35
BanII G_rGCy'C
Cuts at: 123 191 308 423 581 1072 2102 123
Size: 68 117 115 158 491 1030 2079
Fragments arranged by size:
2079 1030 491 158 117 115 68
BbeI G_GCGC'C
Cuts at: 1492 1740 1492
Size: 248 3810
BciVI GTATCCnnnnn_n'
Cuts at: 1956 2717 4058 1956
Size: 761 1341 1956
Fragments arranged by size:
1956 1341 761
BclI T'GATC_A
Cuts at: 1578 1578
Size: 4058
BglI GCCn_nnn'nGGC
Cuts at: 112 384 112
Size: 272 3786
BglII A'GATC_T
Cuts at: 1573 3610 1573
Size: 2037 2021
BlpI GC'TnA_GC
Cuts at: 1170 1170
Size: 4058
Bme1580I G_kGCm'C
Cuts at: 676 1683 1776 2832 676
Size: 1007 93 1056 1902
Fragments arranged by size:
1902 1056 1007 93
BmrI ACTGGGnnnn_n'
Cuts at: 1270 1351 1686 3830 1270
Size: 81 335 2144 1498
Fragments arranged by size:
2144 1498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 223 2425 223
Size: 2202 1856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 373
Size: 4058
Bpu10I CC'TnA_GC
Cuts at: 365 453 365
Size: 88 3970
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 565 1234 2113 2605 2903 292
Size: 273 669 879 492 298 1447
Fragments arranged by size:
1447 879 669 492 298 273
BsaI GGTCTCn'nnnn_
Cuts at: 784 3762 784
Size: 2978 1080
BsaAI yAC'GTr
Cuts at: 1411 1411
Size: 4058
BsaBI GATnn'nnATC
Cuts at: 6 1572 3609 6
Size: 1566 2037 455
Fragments arranged by size:
2037 1566 455
BsaHI Gr'CG_yC
Cuts at: 157 203 1489 1737 2439 3935 157
Size: 46 1286 248 702 1496 280
Fragments arranged by size:
1496 1286 702 280 248 46
BsaWI w'CCGG_w
Cuts at: 1247 1768 2446 2720 2867 3278 1247
Size: 521 678 274 147 411 2027
Fragments arranged by size:
2027 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 308 486 519 308
Size: 178 33 3847
Fragments arranged by size:
3847 178 33
BseYI C'CCAG_C
Cuts at: 212 1483 2241 2818 3314 212
Size: 1271 758 577 496 956
Fragments arranged by size:
1271 956 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 610 398
Size: 212 3846
BsiEI CG_ry'CG
Cuts at: 104 1600 1646 2854 104
Size: 1496 46 1208 1308
Fragments arranged by size:
1496 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 123 371 581 676 1072 1850 2040 2832 123
Size: 248 210 95 396 778 190 792 1349
Fragments arranged by size:
1349 792 778 396 248 210 190 95
BsmBI CGTCTCn'nnnn_
Cuts at: 194 194
Size: 4058
Bsp1286I G_dGCh'C
Cuts at: 123 191 308 371 423 581 676 1072 1683
Size: 68 117 63 52 158 95 396 611
Cuts at: 1683 1776 1850 2040 2102 2832 123
Size: 93 74 190 62 730 1349
Fragments arranged by size:
1349 730 611 396 190 158 117 95
93 74 68 63 62 52
BspEI T'CCGG_A
Cuts at: 3278 3278
Size: 4058
BspHI T'CATG_A
Cuts at: 3234 3234
Size: 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 207 390 1108 1322 1624 2005 207
Size: 183 718 214 302 381 2260
Fragments arranged by size:
2260 718 381 302 214 183
BsrBI CCG'CTC
Cuts at: 50 2350 2404 3888 4053 50
Size: 2300 54 1484 165 55
Fragments arranged by size:
2300 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1970 1970
Size: 4058
BsrFI r'CCGG_y
Cuts at: 2056 2237 2446 2056
Size: 181 209 3668
Fragments arranged by size:
3668 209 181
BsrGI T'GTAC_A
Cuts at: 856 856
Size: 4058
BssSI C'ACGA_G
Cuts at: 343 416 2329 2687 343
Size: 73 1913 358 1714
Fragments arranged by size:
1913 1714 358 73
BstAPI GCAn_nnn'nTGC
Cuts at: 147 1435 147
Size: 1288 2770
BstBI TT'CG_AA
Cuts at: 2419 2419
Size: 4058
BstXI CCAn_nnnn'nTGG
Cuts at: 701 701
Size: 4058
BstYI r'GATC_y
Cuts at: 1 433 704 1083 1550 1573 1908 2154 3166
Size: 432 271 379 467 23 335 246 1012
Cuts at: 3166 3252 3264 3610 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 432 379 346 335 271
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 354 872 107
Size: 247 518 3293
Fragments arranged by size:
3293 518 247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2008 2189 2352 2008
Size: 181 163 3714
Fragments arranged by size:
3714 181 163
BtsI GCAGTG_nn'
Cuts at: 724 1301 724
Size: 577 3481
DraI TTT'AAA
Cuts at: 1057 1057
Size: 4058
DrdI GACnn_nn'nnGTC
Cuts at: 356 1764 2622 356
Size: 1408 858 1792
Fragments arranged by size:
1792 1408 858
EaeI y'GGCC_r
Cuts at: 1643 1817 2208 2235 3580 1643
Size: 174 391 27 1345 2121
Fragments arranged by size:
2121 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1643 1643
Size: 4058
EarI CTCTTCn'nnn_
Cuts at: 787 2081 2291 787
Size: 1294 210 2554
Fragments arranged by size:
2554 1294 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2266 2576 2722 3868 2266
Size: 310 146 1146 2456
Fragments arranged by size:
2456 1146 310 146
EcoICRI GAG'CTC
Cuts at: 121 579 1070 121
Size: 458 491 3109
Fragments arranged by size:
3109 491 458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1883 2315 3062 3330 373
Size: 1510 432 747 268 1101
Fragments arranged by size:
1510 1101 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 681 681
Size: 4058
EcoO109I rG'GnC_Cy
Cuts at: 280 1197 280
Size: 917 3141
EcoRI G'AATT_C
Cuts at: 1062 1062
Size: 4058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 4058
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4058
HaeII r_GCGC'y
Cuts at: 1492 1740 2762 3741 1492
Size: 248 1022 979 1809
Fragments arranged by size:
1809 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 290 322 1900 1930 1932 1962 290
Size: 32 1578 30 2 30 2386
Fragments arranged by size:
2386 1578 32 30 30 2
HincII GTy'rAC
Cuts at: 966 1097 966
Size: 131 3927
HpaI GTT'AAC
Cuts at: 966 966
Size: 4058
KasI G'GCGC_C
Cuts at: 1488 1736 1488
Size: 248 3810
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4058
MluI A'CGCG_T
Cuts at: 3491 3491
Size: 4058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2729 2913 2729
Size: 184 3874
MscI TGG'CCA
Cuts at: 1819 1819
Size: 4058
MslI CAynn'nnrTG
Cuts at: 2047 2174 2047
Size: 127 3931
MspA1I CmG'CkG
Cuts at: 983 1169 1483 1843 2856 3101 983
Size: 186 314 360 1013 245 1940
Fragments arranged by size:
1940 1013 360 314 245 186
NaeI GCC'GGC
Cuts at: 2239 2239
Size: 4058
NarI GG'CG_CC
Cuts at: 1489 1737 1489
Size: 248 3810
NcoI C'CATG_G
Cuts at: 107 872 107
Size: 765 3293
NgoMIV G'CCGG_C
Cuts at: 2237 2237
Size: 4058
NspI r_CATG'y
Cuts at: 226 1111 1611 2518 226
Size: 885 500 907 1766
Fragments arranged by size:
1766 907 885 500
PciI A'CATG_T
Cuts at: 222 2514 222
Size: 2292 1766
PflMI CCAn_nnn'nTGG
Cuts at: 301 301
Size: 4058
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 4026
PstI C_TGCA'G
Cuts at: 155 1105 1790 155
Size: 950 685 2423
Fragments arranged by size:
2423 950 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4058
PvuII CAG'CTG
Cuts at: 1483 1843 1483
Size: 360 3698
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4058
SacI G_AGCT'C
Cuts at: 123 581 1072 123
Size: 458 491 3109
Fragments arranged by size:
3109 491 458
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4058
SapI GCTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4058
ScaI AGT'ACT
Cuts at: 3575 3575
Size: 4058
SfcI C'TryA_G
Cuts at: 33 151 633 1101 1786 2779 2970 33
Size: 118 482 468 685 993 191 1121
Fragments arranged by size:
1121 993 685 482 468 191 118
SfoI GGC'GCC
Cuts at: 1490 1738 1490
Size: 248 3810
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
SmaI CCC'GGG
Cuts at: 1080 1080
Size: 4058
SmlI C'TyrA_G
Cuts at: 307 580 595 1213 2128 2620 2882 307
Size: 273 15 618 915 492 262 1483
Fragments arranged by size:
1483 915 618 492 273 262 15
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4058
SphI G_CATG'C
Cuts at: 1111 1611 1111
Size: 500 3558
StuI AGG'CCT
Cuts at: 687 972 687
Size: 285 3773
StyI C'CwwG_G
Cuts at: 107 382 682 872 923 1030 1192 1546 107
Size: 275 300 190 51 107 162 354 2619
Fragments arranged by size:
2619 354 300 275 190 162 107 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 226 865 1641 1774 2233 2442 226
Size: 639 776 133 459 209 1842
Fragments arranged by size:
1842 776 639 459 209 133
TatI w'GTAC_w
Cuts at: 269 856 3573 269
Size: 587 2717 754
Fragments arranged by size:
2717 754 587
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 4026
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4058
XbaI T'CTAG_A
Cuts at: 1089 1089
Size: 4058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1553 1553
Size: 4058
XmaI C'CCGG_G
Cuts at: 1078 1078
Size: 4058
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsaXI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI