Restriction map for
pF1KB9823
(Circular) (Six-base) MAPSORT of: pF1KB9823.seq Check: 6247 from: 1 to: 4055
pF1KB9823 4055 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 655 655
Size: 4055
AccI GT'mk_AC
Cuts at: 1093 1093
Size: 4055
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 543 1880 2312 3059 3327 543
Size: 1337 432 747 268 1271
Fragments arranged by size:
1337 1271 747 432 268
AflIII A'CryG_T
Cuts at: 1407 2511 3488 1407
Size: 1104 977 1974
Fragments arranged by size:
1974 1104 977
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 215 236 215
Size: 21 4034
AlwNI CAG_nnn'CTG
Cuts at: 1360 2927 1360
Size: 1567 2488
ApaI G_GGCC'C
Cuts at: 939 939
Size: 4055
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 531 1059 3408 3664 531
Size: 528 2349 256 922
Fragments arranged by size:
2349 922 528 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 264 1071 1485 1733 1768 264
Size: 807 414 248 35 2551
Fragments arranged by size:
2551 807 414 248 35
BanII G_rGCy'C
Cuts at: 367 939 1069 2099 367
Size: 572 130 1030 2323
Fragments arranged by size:
2323 1030 572 130
BbeI G_GCGC'C
Cuts at: 1489 1737 1489
Size: 248 3807
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4021
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 1575 1575
Size: 4055
BglI GCCn_nnn'nGGC
Cuts at: 677 677
Size: 4055
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 698 756 939 1680 1773 2829 698
Size: 58 183 741 93 1056 1924
Fragments arranged by size:
1924 1056 741 183 93 58
BmrI ACTGGGnnnn_n'
Cuts at: 1267 1348 1683 3827 1267
Size: 81 335 2144 1495
Fragments arranged by size:
2144 1495 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 240 2422 240
Size: 2182 1873
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 366 430 895 351
Size: 15 64 465 3511
Fragments arranged by size:
3511 465 64 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 175 457 1231 2110 2602 2900 136
Size: 39 282 774 879 492 298 1291
Fragments arranged by size:
1291 879 774 492 298 282 39
BsaI GGTCTCn'nnnn_
Cuts at: 3759 3759
Size: 4055
BsaAI yAC'GTr
Cuts at: 1408 1408
Size: 4055
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 1486 1734 2436 3932 1486
Size: 248 702 1496 1609
Fragments arranged by size:
1609 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 138 216 219 222 223 460 775 790 138
Size: 78 3 3 1 237 315 15 3403
Fragments arranged by size:
3403 315 237 78 15 3 3 1
BseYI C'CCAG_C
Cuts at: 513 675 1480 2238 2815 3311 513
Size: 162 805 758 577 496 1257
Fragments arranged by size:
1257 805 758 577 496 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 952 880
Size: 72 3983
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1069 1847 2037 2829 1069
Size: 778 190 792 2295
Fragments arranged by size:
2295 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 406 406
Size: 4055
Bsp1286I G_dGCh'C
Cuts at: 367 698 756 939 1069 1680 1773 1847 2037
Size: 331 58 183 130 611 93 74 190
Cuts at: 2037 2099 2829 367
Size: 62 730 1593
Fragments arranged by size:
1593 730 611 331 190 183 130 93
74 62 58
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 3231 3231
Size: 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 655 876 1105 1319 1621 2002 655
Size: 221 229 214 302 381 2708
Fragments arranged by size:
2708 381 302 229 221 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1967 1967
Size: 4055
BsrFI r'CCGG_y
Cuts at: 881 2053 2234 2443 881
Size: 1172 181 209 2493
Fragments arranged by size:
2493 1172 209 181
BsrGI T'GTAC_A
Cuts at: 632 632
Size: 4055
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 975 1432 975
Size: 457 3598
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstYI r'GATC_y
Cuts at: 1 1080 1547 1570 1905 2151 3163 3249 3261
Size: 1079 467 23 335 246 1012 86 12
Cuts at: 3261 3607 1
Size: 346 449
Fragments arranged by size:
1079 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 143 302 758 143
Size: 159 456 3440
Fragments arranged by size:
3440 456 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2005 2186 2349 2005
Size: 181 163 3711
Fragments arranged by size:
3711 181 163
BtsI GCAGTG_nn'
Cuts at: 132 903 1298 132
Size: 771 395 2889
Fragments arranged by size:
2889 771 395
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 624 725 1640 1814 2205 2232 3577 624
Size: 101 915 174 391 27 1345 1102
Fragments arranged by size:
1345 1102 915 391 174 101 27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 1040 2078 2288 1040
Size: 1038 210 2807
Fragments arranged by size:
2807 1038 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 366 430 543 895 1880 2312 3059 3327
Size: 15 64 113 352 985 432 747 268
Cuts at: 3327 351
Size: 1079
Fragments arranged by size:
1079 985 747 432 352 268 113 64
15
EcoNI CCTnn'n_nnAGG
Cuts at: 146 259 146
Size: 113 3942
EcoO109I rG'GnC_Cy
Cuts at: 171 888 935 936 994 1194 171
Size: 717 47 1 58 200 3032
Fragments arranged by size:
3032 717 200 58 47 1
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 4055
FspI TGC'GCA
Cuts at: 191 827 1836 191
Size: 636 1009 2410
Fragments arranged by size:
2410 1009 636
HaeII r_GCGC'y
Cuts at: 1489 1737 2759 3738 1489
Size: 248 1022 979 1806
Fragments arranged by size:
1806 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 1897 1927 1929 1959 349
Size: 32 1516 30 2 30 2445
Fragments arranged by size:
2445 1516 32 30 30 2
HincII GTy'rAC
Cuts at: 1094 1094
Size: 4055
KasI G'GCGC_C
Cuts at: 1485 1733 1485
Size: 248 3807
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 770 2726 2910 770
Size: 1956 184 1915
Fragments arranged by size:
1956 1915 184
MscI TGG'CCA
Cuts at: 727 1816 727
Size: 1089 2966
MslI CAynn'nnrTG
Cuts at: 601 2044 2171 601
Size: 1443 127 2485
Fragments arranged by size:
2485 1443 127
MspA1I CmG'CkG
Cuts at: 585 1166 1480 1840 2853 3098 585
Size: 581 314 360 1013 245 1542
Fragments arranged by size:
1542 1013 581 360 314 245
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 4055
NarI GG'CG_CC
Cuts at: 1486 1734 1486
Size: 248 3807
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 4055
NspI r_CATG'y
Cuts at: 911 1108 1608 2515 911
Size: 197 500 907 2451
Fragments arranged by size:
2451 907 500 197
PasI CC'CwG_GG
Cuts at: 174 174
Size: 4055
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PflMI CCAn_nnn'nTGG
Cuts at: 855 855
Size: 4055
PfoI T'CCnGG_A
Cuts at: 536 536
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PpuMI rG'GwC_Cy
Cuts at: 888 994 888
Size: 106 3949
PspOMI G'GGCC_C
Cuts at: 935 935
Size: 4055
PstI C_TGCA'G
Cuts at: 1102 1787 1102
Size: 685 3370
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 585 1480 1840 585
Size: 895 360 2800
Fragments arranged by size:
2800 895 360
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 577 3572 577
Size: 2995 1060
SfcI C'TryA_G
Cuts at: 33 1098 1783 2776 2967 33
Size: 1065 685 993 191 1121
Fragments arranged by size:
1121 1065 993 685 191
SfoI GGC'GCC
Cuts at: 1487 1735 1487
Size: 248 3807
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 115 154 472 1210 2125 2617 2879 115
Size: 39 318 738 915 492 262 1291
Fragments arranged by size:
1291 915 738 492 318 262 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 911 1108 1608 911
Size: 197 500 3358
Fragments arranged by size:
3358 500 197
StuI AGG'CCT
Cuts at: 152 707 152
Size: 555 3500
StyI C'CwwG_G
Cuts at: 162 931 1189 1543 162
Size: 769 258 354 2674
Fragments arranged by size:
2674 769 354 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 175 862 1638 1771 2230 2439 175
Size: 687 776 133 459 209 1791
Fragments arranged by size:
1791 776 687 459 209 133
TatI w'GTAC_w
Cuts at: 575 632 3570 575
Size: 57 2938 1060
Fragments arranged by size:
2938 1060 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4023
Tth111I GACn'n_nGTC
Cuts at: 817 1852 817
Size: 1035 3020
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1550 1550
Size: 4055
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI