Restriction map for
pF1KB9821
(Circular) (Six-base) MAPSORT of: pF1KB9821.seq Check: 6970 from: 1 to: 4049
pF1KB9821 4049 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1087 1087
Size: 4049
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
AflIII A'CryG_T
Cuts at: 1401 2505 3482 1401
Size: 1104 977 1968
Fragments arranged by size:
1968 1104 977
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AlwNI CAG_nnn'CTG
Cuts at: 893 1354 2921 893
Size: 461 1567 2021
Fragments arranged by size:
2021 1567 461
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 1053 3402 3658 1053
Size: 2349 256 1444
Fragments arranged by size:
2349 1444 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 561 1069 561
Size: 508 3541
BamHI G'GATC_C
Cuts at: 1074 1074
Size: 4049
BanI G'GyrC_C
Cuts at: 1023 1065 1479 1727 1762 1023
Size: 42 414 248 35 3310
Fragments arranged by size:
3310 414 248 42 35
BanII G_rGCy'C
Cuts at: 310 358 409 856 916 1063 2093 310
Size: 48 51 447 60 147 1030 2266
Fragments arranged by size:
2266 1030 447 147 60 51 48
BbeI G_GCGC'C
Cuts at: 1027 1483 1731 1027
Size: 456 248 3345
Fragments arranged by size:
3345 456 248
BbsI GAAGACnn'nnnn_
Cuts at: 166 166
Size: 4049
BbvCI CC'TCA_GC
Cuts at: 137 137
Size: 4049
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 1569 1569
Size: 4049
BglI GCCn_nnn'nGGC
Cuts at: 1032 1032
Size: 4049
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 1674 1767 2823 1674
Size: 93 1056 2900
Fragments arranged by size:
2900 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 139 1261 1342 1677 3821 139
Size: 1122 81 335 2144 367
Fragments arranged by size:
2144 1122 367 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 480 624 816 959 405
Size: 75 144 192 143 3495
Fragments arranged by size:
3495 192 144 143 75
Bpu10I CC'TnA_GC
Cuts at: 137 231 137
Size: 94 3955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2104 2596 2894 1225
Size: 879 492 298 2380
Fragments arranged by size:
2380 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 4049
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 596 989 1024 1480 1728 2430 3926 596
Size: 393 35 456 248 702 1496 719
Fragments arranged by size:
1496 719 702 456 393 248 35
BsaWI w'CCGG_w
Cuts at: 358 379 454 1238 1759 2437 2711 2858 3269
Size: 21 75 784 521 678 274 147 411
Cuts at: 3269 358
Size: 1138
Fragments arranged by size:
1138 784 678 521 411 274 147 75
21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 300 384 441 459 504 531 603 696 726
Size: 84 57 18 45 27 72 93 30
Cuts at: 726 864 948 300
Size: 138 84 3401
Fragments arranged by size:
3401 138 93 84 84 72 57 45
30 27 18
BseYI C'CCAG_C
Cuts at: 770 1010 1474 2232 2809 3305 770
Size: 240 464 758 577 496 1514
Fragments arranged by size:
1514 758 577 496 464 240
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 856 1063 1841 2031 2823 856
Size: 207 778 190 792 2082
Fragments arranged by size:
2082 792 778 207 190
BsmBI CGTCTCn'nnnn_
Cuts at: 603 738 603
Size: 135 3914
Bsp1286I G_dGCh'C
Cuts at: 310 358 409 856 916 1063 1674 1767 1841
Size: 48 51 447 60 147 611 93 74
Cuts at: 1841 2031 2093 2823 310
Size: 190 62 730 1536
Fragments arranged by size:
1536 730 611 447 190 147 93 74
62 60 51 48
BspEI T'CCGG_A
Cuts at: 379 3269 379
Size: 2890 1159
BspHI T'CATG_A
Cuts at: 3225 3225
Size: 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 735 1099 1313 1615 1996 735
Size: 364 214 302 381 2788
Fragments arranged by size:
2788 381 364 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1961 1961
Size: 4049
BsrFI r'CCGG_y
Cuts at: 2047 2228 2437 2047
Size: 181 209 3659
Fragments arranged by size:
3659 209 181
BssHII G'CGCG_C
Cuts at: 764 764
Size: 4049
BssSI C'ACGA_G
Cuts at: 759 2320 2678 759
Size: 1561 358 2130
Fragments arranged by size:
2130 1561 358
BstAPI GCAn_nnn'nTGC
Cuts at: 893 1426 893
Size: 533 3516
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstYI r'GATC_y
Cuts at: 1 1074 1541 1564 1899 2145 3157 3243 3255
Size: 1073 467 23 335 246 1012 86 12
Cuts at: 3255 3601 1
Size: 346 449
Fragments arranged by size:
1073 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 962 962
Size: 4049
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 1292 1292
Size: 4049
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 1033 1634 1808 2199 2226 3571 1033
Size: 601 174 391 27 1345 1511
Fragments arranged by size:
1511 1345 601 391 174 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 713 830 2257 2567 2713 3859 713
Size: 117 1427 310 146 1146 903
Fragments arranged by size:
1427 1146 903 310 146 117
EcoICRI GAG'CTC
Cuts at: 854 1061 854
Size: 207 3842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 480 624 816 959 1874 2306 3053 3321
Size: 75 144 192 143 915 432 747 268
Cuts at: 3321 405
Size: 1133
Fragments arranged by size:
1133 915 747 432 268 192 144 143
75
EcoO109I rG'GnC_Cy
Cuts at: 1188 1188
Size: 4049
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 1027 1483 1731 2753 3732 1027
Size: 456 248 1022 979 1344
Fragments arranged by size:
1344 1022 979 456 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1891 1921 1923 1953 1891
Size: 30 2 30 3987
Fragments arranged by size:
3987 30 30 2
HincII GTy'rAC
Cuts at: 1088 1088
Size: 4049
KasI G'GCGC_C
Cuts at: 1023 1479 1727 1023
Size: 456 248 3345
Fragments arranged by size:
3345 456 248
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2904 2720
Size: 184 3865
MscI TGG'CCA
Cuts at: 1810 1810
Size: 4049
MslI CAynn'nnrTG
Cuts at: 2038 2165 2038
Size: 127 3922
MspA1I CmG'CkG
Cuts at: 194 248 425 500 644 719 812 836 890
Size: 54 177 75 144 75 93 24 54
Cuts at: 890 1160 1474 1834 2847 3092 194
Size: 270 314 360 1013 245 1151
Fragments arranged by size:
1151 1013 360 314 270 245 177 144
93 75 75 54 54 24
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 1024 1480 1728 1024
Size: 456 248 3345
Fragments arranged by size:
3345 456 248
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NspI r_CATG'y
Cuts at: 1102 1602 2509 1102
Size: 500 907 2642
Fragments arranged by size:
2642 907 500
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PfoI T'CCnGG_A
Cuts at: 877 973 877
Size: 96 3953
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 234 266 234
Size: 32 4017
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 1474 1834 1474
Size: 360 3689
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 856 1063 856
Size: 207 3842
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 376 3566 376
Size: 3190 859
SfcI C'TryA_G
Cuts at: 33 1092 1777 2770 2961 33
Size: 1059 685 993 191 1121
Fragments arranged by size:
1121 1059 993 685 191
SfoI GGC'GCC
Cuts at: 1025 1481 1729 1025
Size: 456 248 3345
Fragments arranged by size:
3345 456 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 1071 1071
Size: 4049
SmlI C'TyrA_G
Cuts at: 1204 2119 2611 2873 1204
Size: 915 492 262 2380
Fragments arranged by size:
2380 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
StyI C'CwwG_G
Cuts at: 1183 1537 1183
Size: 354 3695
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 955 1632 1765 2224 2433 955
Size: 677 133 459 209 2571
Fragments arranged by size:
2571 677 459 209 133
TatI w'GTAC_w
Cuts at: 374 3564 374
Size: 3190 859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 4017
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 1069 1069
Size: 4049
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI