Restriction map for
pF1KB9820
(Circular) (Six-base) MAPSORT of: pF1KB9820.seq Check: 5520 from: 1 to: 4046
pF1KB9820 4046 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1084 1084
Size: 4046
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1871 2303 3050 3318 1871
Size: 432 747 268 2599
Fragments arranged by size:
2599 747 432 268
AflII C'TTAA_G
Cuts at: 877 877
Size: 4046
AflIII A'CryG_T
Cuts at: 1398 2502 3479 1398
Size: 1104 977 1965
Fragments arranged by size:
1965 1104 977
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AhdI GACnn_n'nnGTC
Cuts at: 462 462
Size: 4046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 260 260
Size: 4046
AlwNI CAG_nnn'CTG
Cuts at: 1351 2918 1351
Size: 1567 2479
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 133 1050 3399 3655 133
Size: 917 2349 256 524
Fragments arranged by size:
2349 917 524 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 1066 1066
Size: 4046
BamHI G'GATC_C
Cuts at: 1071 1071
Size: 4046
BanI G'GyrC_C
Cuts at: 864 935 1062 1476 1724 1759 864
Size: 71 127 414 248 35 3151
Fragments arranged by size:
3151 414 248 127 71 35
BanII G_rGCy'C
Cuts at: 569 1060 2090 569
Size: 491 1030 2525
Fragments arranged by size:
2525 1030 491
BbeI G_GCGC'C
Cuts at: 1480 1728 1480
Size: 248 3798
BbvCI CC'TCA_GC
Cuts at: 440 584 440
Size: 144 3902
BciVI GTATCCnnnnn_n'
Cuts at: 553 1944 2705 4046 553
Size: 1391 761 1341 553
Fragments arranged by size:
1391 1341 761 553
BclI T'GATC_A
Cuts at: 1566 1566
Size: 4046
BglI GCCn_nnn'nGGC
Cuts at: 377 377
Size: 4046
BglII A'GATC_T
Cuts at: 758 1561 3598 758
Size: 803 2037 1206
Fragments arranged by size:
2037 1206 803
BlpI GC'TnA_GC
Cuts at: 233 1158 233
Size: 925 3121
Bme1580I G_kGCm'C
Cuts at: 373 938 1671 1764 2820 373
Size: 565 733 93 1056 1599
Fragments arranged by size:
1599 1056 733 565 93
BmrI ACTGGGnnnn_n'
Cuts at: 186 296 395 548 1258 1339 1674 3818 186
Size: 110 99 153 710 81 335 2144 414
Fragments arranged by size:
2144 710 414 335 153 110 99 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 495 592 495
Size: 97 3949
Bpu10I CC'TnA_GC
Cuts at: 440 584 966 440
Size: 144 382 3520
Fragments arranged by size:
3520 382 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2101 2593 2891 1222
Size: 879 492 298 2377
Fragments arranged by size:
2377 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 291 3750 291
Size: 3459 587
BsaAI yAC'GTr
Cuts at: 1399 1399
Size: 4046
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 1477 1725 2427 3923 1477
Size: 248 702 1496 1600
Fragments arranged by size:
1600 1496 702 248
BsaWI w'CCGG_w
Cuts at: 804 1235 1756 2434 2708 2855 3266 804
Size: 431 521 678 274 147 411 1584
Fragments arranged by size:
1584 678 521 431 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 288 318 586 616 288
Size: 30 268 30 3718
Fragments arranged by size:
3718 268 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 309 592 967 309
Size: 283 375 3388
Fragments arranged by size:
3388 375 283
BseYI C'CCAG_C
Cuts at: 1471 2229 2806 3302 1471
Size: 758 577 496 2215
Fragments arranged by size:
2215 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 772
Size: 4046
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 226 569 823 1060 1838 2028 2820 226
Size: 343 254 237 778 190 792 1452
Fragments arranged by size:
1452 792 778 343 254 237 190
Bsp1286I G_dGCh'C
Cuts at: 226 373 569 823 938 1060 1671 1764 1838
Size: 147 196 254 115 122 611 93 74
Cuts at: 1838 2028 2090 2820 226
Size: 190 62 730 1452
Fragments arranged by size:
1452 730 611 254 196 190 147 122
115 93 74 62
BspEI T'CCGG_A
Cuts at: 804 3266 804
Size: 2462 1584
BspHI T'CATG_A
Cuts at: 3222 3222
Size: 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1096 1310 1612 1993 1096
Size: 214 302 381 3149
Fragments arranged by size:
3149 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2338 2392 3876 4041 50
Size: 2288 54 1484 165 55
Fragments arranged by size:
2288 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1958 1958
Size: 4046
BsrFI r'CCGG_y
Cuts at: 2044 2225 2434 2044
Size: 181 209 3656
Fragments arranged by size:
3656 209 181
BssSI C'ACGA_G
Cuts at: 959 2317 2675 959
Size: 1358 358 2330
Fragments arranged by size:
2330 1358 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 1423
Size: 4046
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstYI r'GATC_y
Cuts at: 1 414 758 1071 1538 1561 1896 2142 3154
Size: 413 344 313 467 23 335 246 1012
Cuts at: 3154 3240 3252 3598 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 413 346 344 335 313
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 150 107
Size: 43 4003
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 1289 1289
Size: 4046
DraI TTT'AAA
Cuts at: 1045 1045
Size: 4046
DrdI GACnn_nn'nnGTC
Cuts at: 1752 2610 1752
Size: 858 3188
EaeI y'GGCC_r
Cuts at: 153 1631 1805 2196 2223 3568 153
Size: 1478 174 391 27 1345 631
Fragments arranged by size:
1478 1345 631 391 174 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 286 569 811 2069 2279 286
Size: 283 242 1258 210 2053
Fragments arranged by size:
2053 1258 283 242 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2254 2564 2710 3856 2254
Size: 310 146 1146 2444
Fragments arranged by size:
2444 1146 310 146
EcoICRI GAG'CTC
Cuts at: 567 1058 567
Size: 491 3555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 495 592 1871 2303 3050 3318 495
Size: 97 1279 432 747 268 1223
Fragments arranged by size:
1279 1223 747 432 268 97
EcoNI CCTnn'n_nnAGG
Cuts at: 678 678
Size: 4046
EcoO109I rG'GnC_Cy
Cuts at: 159 390 683 1185 159
Size: 231 293 502 3020
Fragments arranged by size:
3020 502 293 231
EcoRI G'AATT_C
Cuts at: 1050 1050
Size: 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 4046
FspI TGC'GCA
Cuts at: 1827 1827
Size: 4046
HaeII r_GCGC'y
Cuts at: 1480 1728 2750 3729 1480
Size: 248 1022 979 1797
Fragments arranged by size:
1797 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 208 240 288 320 448 480 586 618 1888
Size: 32 48 32 128 32 106 32 1270
Cuts at: 1888 1918 1920 1950 208
Size: 30 2 30 2304
Fragments arranged by size:
2304 1270 128 106 48 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1022 1085 1022
Size: 63 3983
KasI G'GCGC_C
Cuts at: 1476 1724 1476
Size: 248 3798
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MfeI C'AATT_G
Cuts at: 693 693
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 240 2717 2901 240
Size: 2477 184 1385
Fragments arranged by size:
2477 1385 184
MscI TGG'CCA
Cuts at: 155 1807 155
Size: 1652 2394
MslI CAynn'nnrTG
Cuts at: 2035 2162 2035
Size: 127 3919
MspA1I CmG'CkG
Cuts at: 1157 1471 1831 2844 3089 1157
Size: 314 360 1013 245 2114
Fragments arranged by size:
2114 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 1477 1725 1477
Size: 248 3798
NcoI C'CATG_G
Cuts at: 107 150 107
Size: 43 4003
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NspI r_CATG'y
Cuts at: 1099 1599 2506 1099
Size: 500 907 2639
Fragments arranged by size:
2639 907 500
PasI CC'CwG_GG
Cuts at: 163 163
Size: 4046
PciI A'CATG_T
Cuts at: 2502 2502
Size: 4046
PflMI CCAn_nnn'nTGG
Cuts at: 341 341
Size: 4046
PfoI T'CCnGG_A
Cuts at: 845 845
Size: 4046
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PpuMI rG'GwC_Cy
Cuts at: 159 159
Size: 4046
PstI C_TGCA'G
Cuts at: 1093 1778 1093
Size: 685 3361
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 1471 1831 1471
Size: 360 3686
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 569 1060 569
Size: 491 3555
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 811 2069 2279 811
Size: 1258 210 2578
Fragments arranged by size:
2578 1258 210
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SfcI C'TryA_G
Cuts at: 33 1089 1774 2767 2958 33
Size: 1056 685 993 191 1121
Fragments arranged by size:
1121 1056 993 685 191
SfoI GGC'GCC
Cuts at: 1478 1726 1478
Size: 248 3798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 877 1201 2116 2608 2870 877
Size: 324 915 492 262 2053
Fragments arranged by size:
2053 915 492 324 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
StyI C'CwwG_G
Cuts at: 107 150 375 1180 1534 107
Size: 43 225 805 354 2619
Fragments arranged by size:
2619 805 354 225 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 339 1629 1762 2221 2430 339
Size: 1290 133 459 209 1955
Fragments arranged by size:
1955 1290 459 209 133
TatI w'GTAC_w
Cuts at: 3561 3561
Size: 4046
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4014
Tth111I GACn'n_nGTC
Cuts at: 302 1843 302
Size: 1541 2505
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1541 1541
Size: 4046
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AloI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI