Restriction map for
pF1KB9819
(Circular) (Six-base) MAPSORT of: pF1KB9819.seq Check: 4871 from: 1 to: 4043
pF1KB9819 4043 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 548 932 548
Size: 384 3659
AccI GT'mk_AC
Cuts at: 1081 1081
Size: 4043
Acc65I G'GTAC_C
Cuts at: 1059 1059
Size: 4043
AclI AA'CG_TT
Cuts at: 3883 3883
Size: 4043
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1868 2300 3047 3315 1868
Size: 432 747 268 2596
Fragments arranged by size:
2596 747 432 268
AflIII A'CryG_T
Cuts at: 1035 1395 2499 3476 1035
Size: 360 1104 977 1602
Fragments arranged by size:
1602 1104 977 360
AgeI A'CCGG_T
Cuts at: 2431 2431
Size: 4043
AleI CACnn'nnGTG
Cuts at: 674 674
Size: 4043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 692 692
Size: 4043
AlwNI CAG_nnn'CTG
Cuts at: 1348 2915 1348
Size: 1567 2476
ApaI G_GGCC'C
Cuts at: 197 197
Size: 4043
ApaLI G'TGCA_C
Cuts at: 535 2813 535
Size: 2278 1765
ApoI r'AATT_y
Cuts at: 1047 3396 3652 1047
Size: 2349 256 1438
Fragments arranged by size:
2349 1438 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4043
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
AvaI C'yCGr_G
Cuts at: 433 832 982 1063 433
Size: 399 150 81 3413
Fragments arranged by size:
3413 399 150 81
BamHI G'GATC_C
Cuts at: 1068 1068
Size: 4043
BanI G'GyrC_C
Cuts at: 676 728 986 1059 1473 1721 1756 676
Size: 52 258 73 414 248 35 2963
Fragments arranged by size:
2963 414 258 248 73 52 35
BanII G_rGCy'C
Cuts at: 197 409 914 1057 2087 197
Size: 212 505 143 1030 2153
Fragments arranged by size:
2153 1030 505 212 143
BbeI G_GCGC'C
Cuts at: 990 1477 1725 990
Size: 487 248 3308
Fragments arranged by size:
3308 487 248
BciVI GTATCCnnnnn_n'
Cuts at: 1941 2702 4043 1941
Size: 761 1341 1941
Fragments arranged by size:
1941 1341 761
BclI T'GATC_A
Cuts at: 857 1563 857
Size: 706 3337
BfrBI ATG'CAT
Cuts at: 380 380
Size: 4043
BglI GCCn_nnn'nGGC
Cuts at: 264 596 737 792 984 264
Size: 332 141 55 192 3323
Fragments arranged by size:
3323 332 192 141 55
BglII A'GATC_T
Cuts at: 1558 3595 1558
Size: 2037 2006
BlpI GC'TnA_GC
Cuts at: 634 694 1155 634
Size: 60 461 3522
Fragments arranged by size:
3522 461 60
Bme1580I G_kGCm'C
Cuts at: 197 539 1668 1761 2817 197
Size: 342 1129 93 1056 1423
Fragments arranged by size:
1423 1129 1056 342 93
BmgBI CAC'GTC
Cuts at: 240 240
Size: 4043
BmrI ACTGGGnnnn_n'
Cuts at: 513 1255 1336 1671 3815 513
Size: 742 81 335 2144 741
Fragments arranged by size:
2144 742 741 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 624 571
Size: 53 3990
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 2098 2590 2888 1219
Size: 879 492 298 2374
Fragments arranged by size:
2374 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 213 3747 213
Size: 3534 509
BsaAI yAC'GTr
Cuts at: 672 1396 672
Size: 724 3319
BsaBI GATnn'nnATC
Cuts at: 6 1557 3594 6
Size: 1551 2037 455
Fragments arranged by size:
2037 1551 455
BsaHI Gr'CG_yC
Cuts at: 578 987 1474 1722 2424 3920 578
Size: 409 487 248 702 1496 701
Fragments arranged by size:
1496 702 701 487 409 248
BsaWI w'CCGG_w
Cuts at: 1232 1753 2431 2705 2852 3263 1232
Size: 521 678 274 147 411 2012
Fragments arranged by size:
2012 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 205 235 466 496 963 993 1013 1043 205
Size: 30 231 30 467 30 20 30 3205
Fragments arranged by size:
3205 467 231 30 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 958 1024 1027 958
Size: 66 3 3974
Fragments arranged by size:
3974 66 3
BseYI C'CCAG_C
Cuts at: 314 1000 1468 2226 2803 3299 314
Size: 686 468 758 577 496 1058
Fragments arranged by size:
1058 758 686 577 496 468
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 526 910 325
Size: 201 384 3458
Fragments arranged by size:
3458 384 201
BsiEI CG_ry'CG
Cuts at: 104 164 600 899 1585 1631 2839 104
Size: 60 436 299 686 46 1208 1308
Fragments arranged by size:
1308 1208 686 436 299 60 46
BsiHKAI G_wGCw'C
Cuts at: 539 914 1057 1835 2025 2817 539
Size: 375 143 778 190 792 1765
Fragments arranged by size:
1765 792 778 375 190 143
BsmI GAATG_Cn'
Cuts at: 358 382 358
Size: 24 4019
Bsp1286I G_dGCh'C
Cuts at: 197 409 539 914 1057 1668 1761 1835 2025
Size: 212 130 375 143 611 93 74 190
Cuts at: 2025 2087 2817 197
Size: 62 730 1423
Fragments arranged by size:
1423 730 611 375 212 190 143 130
93 74 62
BspEI T'CCGG_A
Cuts at: 3263 3263
Size: 4043
BspHI T'CATG_A
Cuts at: 3219 3219
Size: 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 548 932 1093 1307 1609 1990 449
Size: 99 384 161 214 302 381 2502
Fragments arranged by size:
2502 384 381 302 214 161 99
BsrBI CCG'CTC
Cuts at: 50 2335 2389 3873 4038 50
Size: 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1955 1955
Size: 4043
BsrFI r'CCGG_y
Cuts at: 545 791 2041 2222 2431 545
Size: 246 1250 181 209 2157
Fragments arranged by size:
2157 1250 246 209 181
BssHII G'CGCG_C
Cuts at: 891 891
Size: 4043
BssSI C'ACGA_G
Cuts at: 343 2314 2672 343
Size: 1971 358 1714
Fragments arranged by size:
1971 1714 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1420 1420
Size: 4043
BstBI TT'CG_AA
Cuts at: 2404 2404
Size: 4043
BstXI CCAn_nnnn'nTGG
Cuts at: 648 648
Size: 4043
BstYI r'GATC_y
Cuts at: 1 1068 1535 1558 1893 2139 3151 3237 3249
Size: 1067 467 23 335 246 1012 86 12
Cuts at: 3249 3595 1
Size: 346 449
Fragments arranged by size:
1067 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 260 260
Size: 4043
BtgI C'CryG_G
Cuts at: 107 158 334 647 107
Size: 51 176 313 3503
Fragments arranged by size:
3503 313 176 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1993 2174 2337 1993
Size: 181 163 3699
Fragments arranged by size:
3699 181 163
BtsI GCAGTG_nn'
Cuts at: 1034 1286 1034
Size: 252 3791
DraI TTT'AAA
Cuts at: 1042 1042
Size: 4043
DraIII CAC_nnn'GTG
Cuts at: 348 348
Size: 4043
DrdI GACnn_nn'nnGTC
Cuts at: 1749 2607 1749
Size: 858 3185
EaeI y'GGCC_r
Cuts at: 161 597 896 1628 1802 2193 2220 3565 161
Size: 436 299 732 174 391 27 1345 639
Fragments arranged by size:
1345 732 639 436 391 299 174 27
EagI C'GGCC_G
Cuts at: 161 597 896 1628 161
Size: 436 299 732 2576
Fragments arranged by size:
2576 732 436 299
EarI CTCTTCn'nnn_
Cuts at: 385 2066 2276 385
Size: 1681 210 2152
Fragments arranged by size:
2152 1681 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 949 2251 2561 2707 3853 949
Size: 1302 310 146 1146 1139
Fragments arranged by size:
1302 1146 1139 310 146
EcoICRI GAG'CTC
Cuts at: 912 1055 912
Size: 143 3900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 624 1868 2300 3047 3315 571
Size: 53 1244 432 747 268 1299
Fragments arranged by size:
1299 1244 747 432 268 53
EcoO109I rG'GnC_Cy
Cuts at: 194 852 1182 194
Size: 658 330 3055
Fragments arranged by size:
3055 658 330
EcoRI G'AATT_C
Cuts at: 1047 1047
Size: 4043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 4043
FspI TGC'GCA
Cuts at: 655 772 1824 655
Size: 117 1052 2874
Fragments arranged by size:
2874 1052 117
HaeII r_GCGC'y
Cuts at: 418 647 990 1477 1725 2747 3726 418
Size: 229 343 487 248 1022 979 735
Fragments arranged by size:
1022 979 735 487 343 248 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 1885 1915 1917 1947 466
Size: 32 1387 30 2 30 2562
Fragments arranged by size:
2562 1387 32 30 30 2
HincII GTy'rAC
Cuts at: 609 1082 609
Size: 473 3570
KasI G'GCGC_C
Cuts at: 986 1473 1721 986
Size: 487 248 3308
Fragments arranged by size:
3308 487 248
KpnI G_GTAC'C
Cuts at: 1063 1063
Size: 4043
MluI A'CGCG_T
Cuts at: 3476 3476
Size: 4043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2714 2898 2714
Size: 184 3859
MscI TGG'CCA
Cuts at: 1804 1804
Size: 4043
MslI CAynn'nnrTG
Cuts at: 674 2032 2159 674
Size: 1358 127 2558
Fragments arranged by size:
2558 1358 127
MspA1I CmG'CkG
Cuts at: 336 807 1154 1468 1828 2841 3086 336
Size: 471 347 314 360 1013 245 1293
Fragments arranged by size:
1293 1013 471 360 347 314 245
NaeI GCC'GGC
Cuts at: 2224 2224
Size: 4043
NarI GG'CG_CC
Cuts at: 987 1474 1722 987
Size: 487 248 3308
Fragments arranged by size:
3308 487 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4043
NgoMIV G'CCGG_C
Cuts at: 2222 2222
Size: 4043
NotI GC'GGCC_GC
Cuts at: 597 597
Size: 4043
NsiI A_TGCA'T
Cuts at: 382 382
Size: 4043
NspI r_CATG'y
Cuts at: 1096 1596 2503 1096
Size: 500 907 2636
Fragments arranged by size:
2636 907 500
PciI A'CATG_T
Cuts at: 2499 2499
Size: 4043
PmeI GTTT'AAAC
Cuts at: 1042 1042
Size: 4043
PmlI CAC'GTG
Cuts at: 672 672
Size: 4043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 4011
PpuMI rG'GwC_Cy
Cuts at: 852 852
Size: 4043
PspOMI G'GGCC_C
Cuts at: 193 193
Size: 4043
PspXI vC'TCGA_Gb
Cuts at: 832 832
Size: 4043
PstI C_TGCA'G
Cuts at: 800 908 1090 1775 800
Size: 108 182 685 3068
Fragments arranged by size:
3068 685 182 108
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4043
PvuII CAG'CTG
Cuts at: 807 1468 1828 807
Size: 661 360 3022
Fragments arranged by size:
3022 661 360
RsrII CG'GwC_CG
Cuts at: 2238 2238
Size: 4043
SacI G_AGCT'C
Cuts at: 914 1057 914
Size: 143 3900
SacII CC_GC'GG
Cuts at: 337 337
Size: 4043
SalI G'TCGA_C
Cuts at: 1080 1080
Size: 4043
SapI GCTCTTCn'nnn_
Cuts at: 385 2066 2276 385
Size: 1681 210 2152
Fragments arranged by size:
2152 1681 210
SbfI CC_TGCA'GG
Cuts at: 1090 1090
Size: 4043
ScaI AGT'ACT
Cuts at: 3560 3560
Size: 4043
SexAI A'CCwGG_T
Cuts at: 116 116
Size: 4043
SfcI C'TryA_G
Cuts at: 33 796 904 1086 1771 2764 2955 33
Size: 763 108 182 685 993 191 1121
Fragments arranged by size:
1121 993 763 685 191 182 108
SfiI GGCCn_nnn'nGGCC
Cuts at: 596 596
Size: 4043
SfoI GGC'GCC
Cuts at: 988 1475 1723 988
Size: 487 248 3308
Fragments arranged by size:
3308 487 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
SmaI CCC'GGG
Cuts at: 984 1065 984
Size: 81 3962
SmlI C'TyrA_G
Cuts at: 832 1198 2113 2605 2867 832
Size: 366 915 492 262 2008
Fragments arranged by size:
2008 915 492 366 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4043
SphI G_CATG'C
Cuts at: 1096 1596 1096
Size: 500 3543
StyI C'CwwG_G
Cuts at: 107 680 1177 1531 107
Size: 573 497 354 2619
Fragments arranged by size:
2619 573 497 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 664 1626 1759 2218 2427 664
Size: 962 133 459 209 2280
Fragments arranged by size:
2280 962 459 209 133
TatI w'GTAC_w
Cuts at: 3558 3558
Size: 4043
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1013 1045 3560 3592 1013
Size: 32 2515 32 1464
Fragments arranged by size:
2515 1464 32 32
Tth111I GACn'n_nGTC
Cuts at: 1840 1840
Size: 4043
XbaI T'CTAG_A
Cuts at: 846 1074 846
Size: 228 3815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1538 1538
Size: 4043
XhoI C'TCGA_G
Cuts at: 832 832
Size: 4043
XmaI C'CCGG_G
Cuts at: 982 1063 982
Size: 81 3962
XmnI GAAnn'nnTTC
Cuts at: 394 394
Size: 4043
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NsiI NspI PciI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BmtI Bpu10I BsiWI BsmBI BsrGI
BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NruI PacI PasI
PflMI PfoI PshAI PsiI PsrI SanDI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI