Restriction map for
pF1KB9809
(Circular) (Six-base) MAPSORT of: pF1KB9809.seq Check: 8486 from: 1 to: 3836
pF1KB9809 3836 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 518 518
Size: 3836
AccI GT'mk_AC
Cuts at: 874 874
Size: 3836
Acc65I G'GTAC_C
Cuts at: 852 852
Size: 3836
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 732 1661 2093 2840 3108 326
Size: 406 929 432 747 268 1054
Fragments arranged by size:
1054 929 747 432 406 268
AflIII A'CryG_T
Cuts at: 1188 2292 3269 1188
Size: 1104 977 1755
Fragments arranged by size:
1755 1104 977
AgeI A'CCGG_T
Cuts at: 2224 2224
Size: 3836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 775 775
Size: 3836
AlwNI CAG_nnn'CTG
Cuts at: 1141 2708 1141
Size: 1567 2269
ApaI G_GGCC'C
Cuts at: 551 551
Size: 3836
ApaLI G'TGCA_C
Cuts at: 2606 2606
Size: 3836
ApoI r'AATT_y
Cuts at: 840 3189 3445 840
Size: 2349 256 1231
Fragments arranged by size:
2349 1231 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 856 856
Size: 3836
BamHI G'GATC_C
Cuts at: 861 861
Size: 3836
BanI G'GyrC_C
Cuts at: 852 1266 1514 1549 852
Size: 414 248 35 3139
Fragments arranged by size:
3139 414 248 35
BanII G_rGCy'C
Cuts at: 551 850 1880 551
Size: 299 1030 2507
Fragments arranged by size:
2507 1030 299
BbeI G_GCGC'C
Cuts at: 1270 1518 1270
Size: 248 3588
BbsI GAAGACnn'nnnn_
Cuts at: 178 379 701 178
Size: 201 322 3313
Fragments arranged by size:
3313 322 201
BciVI GTATCCnnnnn_n'
Cuts at: 1734 2495 3836 1734
Size: 761 1341 1734
Fragments arranged by size:
1734 1341 761
BclI T'GATC_A
Cuts at: 1356 1356
Size: 3836
BglI GCCn_nnn'nGGC
Cuts at: 112 585 112
Size: 473 3363
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 948 948
Size: 3836
Bme1580I G_kGCm'C
Cuts at: 551 1461 1554 2610 551
Size: 910 93 1056 1777
Fragments arranged by size:
1777 1056 910 93
BmrI ACTGGGnnnn_n'
Cuts at: 545 1048 1129 1464 3608 545
Size: 503 81 335 2144 773
Fragments arranged by size:
2144 773 503 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 3836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 713 820 698
Size: 15 107 3714
Fragments arranged by size:
3714 107 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1891 2383 2681 1012
Size: 879 492 298 2167
Fragments arranged by size:
2167 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 130 3540 130
Size: 3410 426
BsaAI yAC'GTr
Cuts at: 1189 1189
Size: 3836
BsaBI GATnn'nnATC
Cuts at: 6 1350 3387 6
Size: 1344 2037 455
Fragments arranged by size:
2037 1344 455
BsaHI Gr'CG_yC
Cuts at: 526 1267 1515 2217 3713 526
Size: 741 248 702 1496 649
Fragments arranged by size:
1496 741 702 649 248
BsaWI w'CCGG_w
Cuts at: 630 658 1025 1546 2224 2498 2645 3056 630
Size: 28 367 521 678 274 147 411 1410
Fragments arranged by size:
1410 678 521 411 367 274 147 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 416 446 416
Size: 30 3806
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 748 748
Size: 3836
BseYI C'CCAG_C
Cuts at: 183 668 1261 2019 2596 3092 183
Size: 485 593 758 577 496 927
Fragments arranged by size:
927 758 593 577 496 485
BsiEI CG_ry'CG
Cuts at: 104 1378 1424 2632 104
Size: 1274 46 1208 1308
Fragments arranged by size:
1308 1274 1208 46
BsiHKAI G_wGCw'C
Cuts at: 254 520 740 850 1628 1818 2610 254
Size: 266 220 110 778 190 792 1480
Fragments arranged by size:
1480 792 778 266 220 190 110
BsmI GAATG_Cn'
Cuts at: 428 428
Size: 3836
Bsp1286I G_dGCh'C
Cuts at: 254 520 551 740 850 1461 1554 1628 1818
Size: 266 31 189 110 611 93 74 190
Cuts at: 1818 1880 2610 254
Size: 62 730 1480
Fragments arranged by size:
1480 730 611 266 190 189 110 93
74 62 31
BspEI T'CCGG_A
Cuts at: 630 3056 630
Size: 2426 1410
BspHI T'CATG_A
Cuts at: 3012 3012
Size: 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 518 886 1100 1402 1783 518
Size: 368 214 302 381 2571
Fragments arranged by size:
2571 381 368 302 214
BsrBI CCG'CTC
Cuts at: 50 2128 2182 3666 3831 50
Size: 2078 54 1484 165 55
Fragments arranged by size:
2078 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1748 1748
Size: 3836
BsrFI r'CCGG_y
Cuts at: 534 1834 2015 2224 534
Size: 1300 181 209 2146
Fragments arranged by size:
2146 1300 209 181
BsrGI T'GTAC_A
Cuts at: 567 567
Size: 3836
BssSI C'ACGA_G
Cuts at: 2107 2465 2107
Size: 358 3478
BstAPI GCAn_nnn'nTGC
Cuts at: 1213 1213
Size: 3836
BstBI TT'CG_AA
Cuts at: 2197 2197
Size: 3836
BstYI r'GATC_y
Cuts at: 1 296 627 861 1328 1351 1686 1932 2944
Size: 295 331 234 467 23 335 246 1012
Cuts at: 2944 3030 3042 3388 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 331 295 246
234 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3836
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 1079 1079
Size: 3836
DraI TTT'AAA
Cuts at: 835 835
Size: 3836
DrdI GACnn_nn'nnGTC
Cuts at: 381 1542 2400 381
Size: 1161 858 1817
Fragments arranged by size:
1817 1161 858
EaeI y'GGCC_r
Cuts at: 474 536 1421 1595 1986 2013 3358 474
Size: 62 885 174 391 27 1345 952
Fragments arranged by size:
1345 952 885 391 174 62 27
EagI C'GGCC_G
Cuts at: 1421 1421
Size: 3836
EarI CTCTTCn'nnn_
Cuts at: 344 1859 2069 344
Size: 1515 210 2111
Fragments arranged by size:
2111 1515 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2044 2354 2500 3646 2044
Size: 310 146 1146 2234
Fragments arranged by size:
2234 1146 310 146
EcoICRI GAG'CTC
Cuts at: 848 848
Size: 3836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 698 713 732 820 1661 2093 2840 3108
Size: 372 15 19 88 841 432 747 268
Cuts at: 3108 326
Size: 1054
Fragments arranged by size:
1054 841 747 432 372 268 88 19
15
EcoO109I rG'GnC_Cy
Cuts at: 547 975 547
Size: 428 3408
EcoRI G'AATT_C
Cuts at: 840 840
Size: 3836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 291 1855 291
Size: 1564 2272
FspI TGC'GCA
Cuts at: 1617 1617
Size: 3836
HaeII r_GCGC'y
Cuts at: 1270 1518 2540 3519 1270
Size: 248 1022 979 1587
Fragments arranged by size:
1587 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 416 448 619 651 1678 1708 1710 1740 416
Size: 32 171 32 1027 30 2 30 2512
Fragments arranged by size:
2512 1027 171 32 32 30 30 2
HincII GTy'rAC
Cuts at: 875 875
Size: 3836
KasI G'GCGC_C
Cuts at: 1266 1514 1266
Size: 248 3588
KpnI G_GTAC'C
Cuts at: 856 856
Size: 3836
MluI A'CGCG_T
Cuts at: 3269 3269
Size: 3836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2691 2507
Size: 184 3652
MscI TGG'CCA
Cuts at: 476 1597 476
Size: 1121 2715
MslI CAynn'nnrTG
Cuts at: 1825 1952 1825
Size: 127 3709
MspA1I CmG'CkG
Cuts at: 118 947 1261 1621 2634 2879 118
Size: 829 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 829 360 314 245
NaeI GCC'GGC
Cuts at: 536 2017 536
Size: 1481 2355
NarI GG'CG_CC
Cuts at: 1267 1515 1267
Size: 248 3588
NcoI C'CATG_G
Cuts at: 107 107
Size: 3836
NgoMIV G'CCGG_C
Cuts at: 534 2015 534
Size: 1481 2355
NspI r_CATG'y
Cuts at: 889 1389 2296 889
Size: 500 907 2429
Fragments arranged by size:
2429 907 500
PciI A'CATG_T
Cuts at: 2292 2292
Size: 3836
PfoI T'CCnGG_A
Cuts at: 434 434
Size: 3836
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3804
PspOMI G'GGCC_C
Cuts at: 547 547
Size: 3836
PstI C_TGCA'G
Cuts at: 515 883 1568 515
Size: 368 685 2783
Fragments arranged by size:
2783 685 368
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3836
PvuII CAG'CTG
Cuts at: 1261 1621 1261
Size: 360 3476
RsrII CG'GwC_CG
Cuts at: 2031 2031
Size: 3836
SacI G_AGCT'C
Cuts at: 850 850
Size: 3836
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 1859 2069 1859
Size: 210 3626
SbfI CC_TGCA'GG
Cuts at: 883 883
Size: 3836
ScaI AGT'ACT
Cuts at: 3353 3353
Size: 3836
SfcI C'TryA_G
Cuts at: 33 511 879 1564 2557 2748 33
Size: 478 368 685 993 191 1121
Fragments arranged by size:
1121 993 685 478 368 191
SfoI GGC'GCC
Cuts at: 1268 1516 1268
Size: 248 3588
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 858 858
Size: 3836
SmlI C'TyrA_G
Cuts at: 991 1906 2398 2660 991
Size: 915 492 262 2167
Fragments arranged by size:
2167 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
StuI AGG'CCT
Cuts at: 334 334
Size: 3836
StyI C'CwwG_G
Cuts at: 107 970 1324 107
Size: 863 354 2619
Fragments arranged by size:
2619 863 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 520 1419 1552 2011 2220 520
Size: 899 133 459 209 2136
Fragments arranged by size:
2136 899 459 209 133
TatI w'GTAC_w
Cuts at: 567 780 3351 567
Size: 213 2571 1052
Fragments arranged by size:
2571 1052 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3353 3385 3353
Size: 32 3804
Tth111I GACn'n_nGTC
Cuts at: 1633 1633
Size: 3836
XbaI T'CTAG_A
Cuts at: 867 867
Size: 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1331 1331
Size: 3836
XmaI C'CCGG_G
Cuts at: 856 856
Size: 3836
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsgI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI