Restriction map for
pF1KB9807
(Circular) (Six-base) MAPSORT of: pF1KB9807.seq Check: 9159 from: 1 to: 3794
pF1KB9807 3794 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 832 832
Size: 3794
Acc65I G'GTAC_C
Cuts at: 810 810
Size: 3794
AclI AA'CG_TT
Cuts at: 3634 3634
Size: 3794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1619 2051 2798 3066 791
Size: 828 432 747 268 1519
Fragments arranged by size:
1519 828 747 432 268
AflIII A'CryG_T
Cuts at: 1146 2250 3227 1146
Size: 1104 977 1713
Fragments arranged by size:
1713 1104 977
AgeI A'CCGG_T
Cuts at: 2182 2182
Size: 3794
AlwNI CAG_nnn'CTG
Cuts at: 1099 2666 1099
Size: 1567 2227
ApaI G_GGCC'C
Cuts at: 756 756
Size: 3794
ApaLI G'TGCA_C
Cuts at: 2564 2564
Size: 3794
ApoI r'AATT_y
Cuts at: 588 616 696 733 798 3147 3403 588
Size: 28 80 37 65 2349 256 979
Fragments arranged by size:
2349 979 256 80 65 37 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 3794
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
AvaI C'yCGr_G
Cuts at: 814 814
Size: 3794
AvrII C'CTAG_G
Cuts at: 718 718
Size: 3794
BamHI G'GATC_C
Cuts at: 819 819
Size: 3794
BanI G'GyrC_C
Cuts at: 810 1224 1472 1507 810
Size: 414 248 35 3097
Fragments arranged by size:
3097 414 248 35
BanII G_rGCy'C
Cuts at: 756 808 1838 756
Size: 52 1030 2712
Fragments arranged by size:
2712 1030 52
BbeI G_GCGC'C
Cuts at: 1228 1476 1228
Size: 248 3546
BbsI GAAGACnn'nnnn_
Cuts at: 224 779 224
Size: 555 3239
BbvCI CC'TCA_GC
Cuts at: 497 497
Size: 3794
BciVI GTATCCnnnnn_n'
Cuts at: 1692 2453 3794 1692
Size: 761 1341 1692
Fragments arranged by size:
1692 1341 761
BclI T'GATC_A
Cuts at: 1314 1314
Size: 3794
BglII A'GATC_T
Cuts at: 1309 3346 1309
Size: 2037 1757
BlpI GC'TnA_GC
Cuts at: 906 906
Size: 3794
Bme1580I G_kGCm'C
Cuts at: 756 1419 1512 2568 756
Size: 663 93 1056 1982
Fragments arranged by size:
1982 1056 663 93
BmrI ACTGGGnnnn_n'
Cuts at: 1006 1087 1422 3566 1006
Size: 81 335 2144 1234
Fragments arranged by size:
2144 1234 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 3794
Bpu10I CC'TnA_GC
Cuts at: 497 497
Size: 3794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 970 1849 2341 2639 541
Size: 429 879 492 298 1696
Fragments arranged by size:
1696 879 492 429 298
BsaI GGTCTCn'nnnn_
Cuts at: 3498 3498
Size: 3794
BsaAI yAC'GTr
Cuts at: 1147 1147
Size: 3794
BsaBI GATnn'nnATC
Cuts at: 6 1308 3345 6
Size: 1302 2037 455
Fragments arranged by size:
2037 1302 455
BsaHI Gr'CG_yC
Cuts at: 1225 1473 2175 3671 1225
Size: 248 702 1496 1348
Fragments arranged by size:
1496 1348 702 248
BsaWI w'CCGG_w
Cuts at: 983 1504 2182 2456 2603 3014 983
Size: 521 678 274 147 411 1763
Fragments arranged by size:
1763 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 492 1219 1977 2554 3050 492
Size: 727 758 577 496 1236
Fragments arranged by size:
1236 758 727 577 496
BsiEI CG_ry'CG
Cuts at: 104 1336 1382 2590 104
Size: 1232 46 1208 1308
Fragments arranged by size:
1308 1232 1208 46
BsiHKAI G_wGCw'C
Cuts at: 808 1586 1776 2568 808
Size: 778 190 792 2034
Fragments arranged by size:
2034 792 778 190
BsmI GAATG_Cn'
Cuts at: 143 143
Size: 3794
Bsp1286I G_dGCh'C
Cuts at: 756 808 1419 1512 1586 1776 1838 2568 756
Size: 52 611 93 74 190 62 730 1982
Fragments arranged by size:
1982 730 611 190 93 74 62 52
BspEI T'CCGG_A
Cuts at: 3014 3014
Size: 3794
BspHI T'CATG_A
Cuts at: 2970 2970
Size: 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1058 1360 1741 844
Size: 214 302 381 2897
Fragments arranged by size:
2897 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2086 2140 3624 3789 50
Size: 2036 54 1484 165 55
Fragments arranged by size:
2036 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 603 1706 603
Size: 1103 2691
BsrFI r'CCGG_y
Cuts at: 1792 1973 2182 1792
Size: 181 209 3404
Fragments arranged by size:
3404 209 181
BssSI C'ACGA_G
Cuts at: 184 2065 2423 184
Size: 1881 358 1555
Fragments arranged by size:
1881 1555 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1171 1171
Size: 3794
BstBI TT'CG_AA
Cuts at: 145 2155 145
Size: 2010 1784
BstYI r'GATC_y
Cuts at: 1 819 1286 1309 1644 1890 2902 2988 3000
Size: 818 467 23 335 246 1012 86 12
Cuts at: 3000 3346 1
Size: 346 449
Fragments arranged by size:
1012 818 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3794
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1744 1925 2088 1744
Size: 181 163 3450
Fragments arranged by size:
3450 181 163
BtsI GCAGTG_nn'
Cuts at: 1037 1037
Size: 3794
DraI TTT'AAA
Cuts at: 793 793
Size: 3794
DrdI GACnn_nn'nnGTC
Cuts at: 1500 2358 1500
Size: 858 2936
EaeI y'GGCC_r
Cuts at: 1379 1553 1944 1971 3316 1379
Size: 174 391 27 1345 1857
Fragments arranged by size:
1857 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1379 1379
Size: 3794
EarI CTCTTCn'nnn_
Cuts at: 124 1817 2027 124
Size: 1693 210 1891
Fragments arranged by size:
1891 1693 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2002 2312 2458 3604 2002
Size: 310 146 1146 2192
Fragments arranged by size:
2192 1146 310 146
EcoICRI GAG'CTC
Cuts at: 806 806
Size: 3794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1619 2051 2798 3066 791
Size: 828 432 747 268 1519
Fragments arranged by size:
1519 828 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 705 752 753 933 705
Size: 47 1 180 3566
Fragments arranged by size:
3566 180 47 1
EcoRI G'AATT_C
Cuts at: 733 798 733
Size: 65 3729
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1813 1813
Size: 3794
FspI TGC'GCA
Cuts at: 1575 1575
Size: 3794
HaeII r_GCGC'y
Cuts at: 492 1228 1476 2498 3477 492
Size: 736 248 1022 979 809
Fragments arranged by size:
1022 979 809 736 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1636 1666 1668 1698 1636
Size: 30 2 30 3732
Fragments arranged by size:
3732 30 30 2
HincII GTy'rAC
Cuts at: 833 833
Size: 3794
KasI G'GCGC_C
Cuts at: 1224 1472 1224
Size: 248 3546
KpnI G_GTAC'C
Cuts at: 814 814
Size: 3794
MluI A'CGCG_T
Cuts at: 3227 3227
Size: 3794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2465 2649 2465
Size: 184 3610
MscI TGG'CCA
Cuts at: 1555 1555
Size: 3794
MslI CAynn'nnrTG
Cuts at: 1783 1910 1783
Size: 127 3667
MspA1I CmG'CkG
Cuts at: 496 905 1219 1579 2592 2837 496
Size: 409 314 360 1013 245 1453
Fragments arranged by size:
1453 1013 409 360 314 245
NaeI GCC'GGC
Cuts at: 1975 1975
Size: 3794
NarI GG'CG_CC
Cuts at: 1225 1473 1225
Size: 248 3546
NcoI C'CATG_G
Cuts at: 107 107
Size: 3794
NgoMIV G'CCGG_C
Cuts at: 1973 1973
Size: 3794
NspI r_CATG'y
Cuts at: 847 1347 2254 847
Size: 500 907 2387
Fragments arranged by size:
2387 907 500
PciI A'CATG_T
Cuts at: 2250 2250
Size: 3794
PmeI GTTT'AAAC
Cuts at: 793 793
Size: 3794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2933 2965 2933
Size: 32 3762
PpuMI rG'GwC_Cy
Cuts at: 705 705
Size: 3794
PspOMI G'GGCC_C
Cuts at: 752 752
Size: 3794
PstI C_TGCA'G
Cuts at: 483 751 841 1526 483
Size: 268 90 685 2751
Fragments arranged by size:
2751 685 268 90
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3794
PvuII CAG'CTG
Cuts at: 496 1219 1579 496
Size: 723 360 2711
Fragments arranged by size:
2711 723 360
RsrII CG'GwC_CG
Cuts at: 1989 1989
Size: 3794
SacI G_AGCT'C
Cuts at: 808 808
Size: 3794
SalI G'TCGA_C
Cuts at: 831 831
Size: 3794
SapI GCTCTTCn'nnn_
Cuts at: 1817 2027 1817
Size: 210 3584
SbfI CC_TGCA'GG
Cuts at: 841 841
Size: 3794
ScaI AGT'ACT
Cuts at: 3311 3311
Size: 3794
SfcI C'TryA_G
Cuts at: 33 479 747 837 1522 2515 2706 33
Size: 446 268 90 685 993 191 1121
Fragments arranged by size:
1121 993 685 446 268 191 90
SfoI GGC'GCC
Cuts at: 1226 1474 1226
Size: 248 3546
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
SmaI CCC'GGG
Cuts at: 816 816
Size: 3794
SmlI C'TyrA_G
Cuts at: 556 949 1864 2356 2618 556
Size: 393 915 492 262 1732
Fragments arranged by size:
1732 915 492 393 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3794
SphI G_CATG'C
Cuts at: 847 1347 847
Size: 500 3294
StyI C'CwwG_G
Cuts at: 107 718 727 928 1282 107
Size: 611 9 201 354 2619
Fragments arranged by size:
2619 611 354 201 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1377 1510 1969 2178 1377
Size: 133 459 209 2993
Fragments arranged by size:
2993 459 209 133
TatI w'GTAC_w
Cuts at: 3309 3309
Size: 3794
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3311 3343 3311
Size: 32 3762
Tth111I GACn'n_nGTC
Cuts at: 1591 1591
Size: 3794
XbaI T'CTAG_A
Cuts at: 825 825
Size: 3794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 540 1289 540
Size: 749 3045
XmaI C'CCGG_G
Cuts at: 814 814
Size: 3794
XmnI GAAnn'nnTTC
Cuts at: 141 776 141
Size: 635 3159
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BcgI BfrBI BglI BmgBI BmtI BpmI
BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI