Restriction map for
pF1KB9802
(Circular) (Six-base) MAPSORT of: pF1KB9802.seq Check: 6854 from: 1 to: 3671
pF1KB9802 3671 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 462 462
Size: 3671
AccI GT'mk_AC
Cuts at: 709 709
Size: 3671
Acc65I G'GTAC_C
Cuts at: 687 687
Size: 3671
AclI AA'CG_TT
Cuts at: 3511 3511
Size: 3671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1496 1928 2675 2943 460
Size: 1036 432 747 268 1188
Fragments arranged by size:
1188 1036 747 432 268
AflIII A'CryG_T
Cuts at: 1023 2127 3104 1023
Size: 1104 977 1590
Fragments arranged by size:
1590 1104 977
AgeI A'CCGG_T
Cuts at: 635 2059 635
Size: 1424 2247
AlwNI CAG_nnn'CTG
Cuts at: 976 2543 976
Size: 1567 2104
ApaLI G'TGCA_C
Cuts at: 2441 2441
Size: 3671
ApoI r'AATT_y
Cuts at: 184 675 3024 3280 184
Size: 491 2349 256 575
Fragments arranged by size:
2349 575 491 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
AvaI C'yCGr_G
Cuts at: 691 691
Size: 3671
BamHI G'GATC_C
Cuts at: 372 696 372
Size: 324 3347
BanI G'GyrC_C
Cuts at: 687 1101 1349 1384 687
Size: 414 248 35 2974
Fragments arranged by size:
2974 414 248 35
BanII G_rGCy'C
Cuts at: 685 1715 685
Size: 1030 2641
BbeI G_GCGC'C
Cuts at: 1105 1353 1105
Size: 248 3423
BciVI GTATCCnnnnn_n'
Cuts at: 1569 2330 3671 1569
Size: 761 1341 1569
Fragments arranged by size:
1569 1341 761
BclI T'GATC_A
Cuts at: 1191 1191
Size: 3671
BglII A'GATC_T
Cuts at: 1186 3223 1186
Size: 2037 1634
BlpI GC'TnA_GC
Cuts at: 434 783 434
Size: 349 3322
Bme1580I G_kGCm'C
Cuts at: 1296 1389 2445 1296
Size: 93 1056 2522
Fragments arranged by size:
2522 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 883 964 1299 3443 883
Size: 81 335 2144 1111
Fragments arranged by size:
2144 1111 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 565 2038 565
Size: 1473 2198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 538
Size: 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1726 2218 2516 847
Size: 879 492 298 2002
Fragments arranged by size:
2002 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3375 3375
Size: 3671
BsaAI yAC'GTr
Cuts at: 1024 1024
Size: 3671
BsaBI GATnn'nnATC
Cuts at: 6 1185 3222 6
Size: 1179 2037 455
Fragments arranged by size:
2037 1179 455
BsaHI Gr'CG_yC
Cuts at: 1102 1350 2052 3548 1102
Size: 248 702 1496 1225
Fragments arranged by size:
1496 1225 702 248
BsaWI w'CCGG_w
Cuts at: 635 860 1381 2059 2333 2480 2891 635
Size: 225 521 678 274 147 411 1415
Fragments arranged by size:
1415 678 521 411 274 225 147
BseYI C'CCAG_C
Cuts at: 1096 1854 2431 2927 1096
Size: 758 577 496 1840
Fragments arranged by size:
1840 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 660 490
Size: 170 3501
BsiEI CG_ry'CG
Cuts at: 104 1213 1259 2467 104
Size: 1109 46 1208 1308
Fragments arranged by size:
1308 1208 1109 46
BsiHKAI G_wGCw'C
Cuts at: 685 1463 1653 2445 685
Size: 778 190 792 1911
Fragments arranged by size:
1911 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 685 1296 1389 1463 1653 1715 2445 685
Size: 611 93 74 190 62 730 1911
Fragments arranged by size:
1911 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2891 2891
Size: 3671
BspHI T'CATG_A
Cuts at: 2847 2847
Size: 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 462 721 935 1237 1618 462
Size: 259 214 302 381 2515
Fragments arranged by size:
2515 381 302 259 214
BsrBI CCG'CTC
Cuts at: 50 1963 2017 3501 3666 50
Size: 1913 54 1484 165 55
Fragments arranged by size:
1913 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1583 1583
Size: 3671
BsrFI r'CCGG_y
Cuts at: 635 1669 1850 2059 635
Size: 1034 181 209 2247
Fragments arranged by size:
2247 1034 209 181
BssSI C'ACGA_G
Cuts at: 1942 2300 1942
Size: 358 3313
BstAPI GCAn_nnn'nTGC
Cuts at: 1048 1048
Size: 3671
BstBI TT'CG_AA
Cuts at: 2032 2032
Size: 3671
BstYI r'GATC_y
Cuts at: 1 256 372 696 1163 1186 1521 1767 2779
Size: 255 116 324 467 23 335 246 1012
Cuts at: 2779 2865 2877 3223 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 324 255 246
116 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 134 134
Size: 3671
BtgI C'CryG_G
Cuts at: 107 107
Size: 3671
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1621 1802 1965 1621
Size: 181 163 3327
Fragments arranged by size:
3327 181 163
BtsI GCAGTG_nn'
Cuts at: 914 914
Size: 3671
DraI TTT'AAA
Cuts at: 222 670 222
Size: 448 3223
DrdI GACnn_nn'nnGTC
Cuts at: 1377 2235 1377
Size: 858 2813
EaeI y'GGCC_r
Cuts at: 296 476 1256 1430 1821 1848 3193 296
Size: 180 780 174 391 27 1345 774
Fragments arranged by size:
1345 780 774 391 180 174 27
EagI C'GGCC_G
Cuts at: 1256 1256
Size: 3671
EarI CTCTTCn'nnn_
Cuts at: 153 418 436 1694 1904 153
Size: 265 18 1258 210 1920
Fragments arranged by size:
1920 1258 265 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1879 2189 2335 3481 1879
Size: 310 146 1146 2069
Fragments arranged by size:
2069 1146 310 146
EcoICRI GAG'CTC
Cuts at: 683 683
Size: 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 538 1496 1928 2675 2943 460
Size: 78 958 432 747 268 1188
Fragments arranged by size:
1188 958 747 432 268 78
EcoO109I rG'GnC_Cy
Cuts at: 810 810
Size: 3671
EcoRI G'AATT_C
Cuts at: 675 675
Size: 3671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1690 1690
Size: 3671
FspI TGC'GCA
Cuts at: 1452 1452
Size: 3671
HaeII r_GCGC'y
Cuts at: 1105 1353 2375 3354 1105
Size: 248 1022 979 1422
Fragments arranged by size:
1422 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1513 1543 1545 1575 1513
Size: 30 2 30 3609
Fragments arranged by size:
3609 30 30 2
HincII GTy'rAC
Cuts at: 503 710 503
Size: 207 3464
KasI G'GCGC_C
Cuts at: 1101 1349 1101
Size: 248 3423
KpnI G_GTAC'C
Cuts at: 691 691
Size: 3671
MluI A'CGCG_T
Cuts at: 3104 3104
Size: 3671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2526 2342
Size: 184 3487
MscI TGG'CCA
Cuts at: 298 478 1432 298
Size: 180 954 2537
Fragments arranged by size:
2537 954 180
MslI CAynn'nnrTG
Cuts at: 530 1660 1787 530
Size: 1130 127 2414
Fragments arranged by size:
2414 1130 127
MspA1I CmG'CkG
Cuts at: 438 782 1096 1456 2469 2714 438
Size: 344 314 360 1013 245 1395
Fragments arranged by size:
1395 1013 360 344 314 245
NaeI GCC'GGC
Cuts at: 1852 1852
Size: 3671
NarI GG'CG_CC
Cuts at: 1102 1350 1102
Size: 248 3423
NcoI C'CATG_G
Cuts at: 107 107
Size: 3671
NgoMIV G'CCGG_C
Cuts at: 1850 1850
Size: 3671
NspI r_CATG'y
Cuts at: 724 1224 2131 724
Size: 500 907 2264
Fragments arranged by size:
2264 907 500
PciI A'CATG_T
Cuts at: 2127 2127
Size: 3671
PmeI GTTT'AAAC
Cuts at: 670 670
Size: 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 566 598 2810 2842 566
Size: 32 2212 32 1395
Fragments arranged by size:
2212 1395 32 32
PstI C_TGCA'G
Cuts at: 718 1403 718
Size: 685 2986
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3671
PvuII CAG'CTG
Cuts at: 438 1096 1456 438
Size: 658 360 2653
Fragments arranged by size:
2653 658 360
RsrII CG'GwC_CG
Cuts at: 1866 1866
Size: 3671
SacI G_AGCT'C
Cuts at: 685 685
Size: 3671
SalI G'TCGA_C
Cuts at: 708 708
Size: 3671
SapI GCTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
SbfI CC_TGCA'GG
Cuts at: 718 718
Size: 3671
ScaI AGT'ACT
Cuts at: 565 3188 565
Size: 2623 1048
SfcI C'TryA_G
Cuts at: 33 714 1399 2392 2583 33
Size: 681 685 993 191 1121
Fragments arranged by size:
1121 993 685 681 191
SfoI GGC'GCC
Cuts at: 1103 1351 1103
Size: 248 3423
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
SmaI CCC'GGG
Cuts at: 693 693
Size: 3671
SmlI C'TyrA_G
Cuts at: 826 1741 2233 2495 826
Size: 915 492 262 2002
Fragments arranged by size:
2002 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3671
SphI G_CATG'C
Cuts at: 724 1224 724
Size: 500 3171
StyI C'CwwG_G
Cuts at: 107 479 805 1159 107
Size: 372 326 354 2619
Fragments arranged by size:
2619 372 354 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1254 1387 1846 2055 1254
Size: 133 459 209 2870
Fragments arranged by size:
2870 459 209 133
TatI w'GTAC_w
Cuts at: 563 3186 563
Size: 2623 1048
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 3639
Tth111I GACn'n_nGTC
Cuts at: 1468 1468
Size: 3671
XbaI T'CTAG_A
Cuts at: 232 702 232
Size: 470 3201
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1166 1166
Size: 3671
XmaI C'CCGG_G
Cuts at: 691 691
Size: 3671
XmnI GAAnn'nnTTC
Cuts at: 240 240
Size: 3671
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI