Restriction map for
pF1KB9801
(Circular) (Six-base) MAPSORT of: pF1KB9801.seq Check: 5068 from: 1 to: 3668
pF1KB9801 3668 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 518 518
Size: 3668
AccI GT'mk_AC
Cuts at: 706 706
Size: 3668
Acc65I G'GTAC_C
Cuts at: 684 684
Size: 3668
AclI AA'CG_TT
Cuts at: 3508 3508
Size: 3668
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 1493 1925 2672 2940 326
Size: 1167 432 747 268 1054
Fragments arranged by size:
1167 1054 747 432 268
AflIII A'CryG_T
Cuts at: 1020 2124 3101 1020
Size: 1104 977 1587
Fragments arranged by size:
1587 1104 977
AgeI A'CCGG_T
Cuts at: 2056 2056
Size: 3668
AlwNI CAG_nnn'CTG
Cuts at: 973 2540 973
Size: 1567 2101
ApaLI G'TGCA_C
Cuts at: 2438 2438
Size: 3668
ApoI r'AATT_y
Cuts at: 672 3021 3277 672
Size: 2349 256 1063
Fragments arranged by size:
2349 1063 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3668
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
AvaI C'yCGr_G
Cuts at: 688 688
Size: 3668
BamHI G'GATC_C
Cuts at: 693 693
Size: 3668
BanI G'GyrC_C
Cuts at: 684 1098 1346 1381 684
Size: 414 248 35 2971
Fragments arranged by size:
2971 414 248 35
BanII G_rGCy'C
Cuts at: 682 1712 682
Size: 1030 2638
BbeI G_GCGC'C
Cuts at: 1102 1350 1102
Size: 248 3420
BbsI GAAGACnn'nnnn_
Cuts at: 178 379 178
Size: 201 3467
BciVI GTATCCnnnnn_n'
Cuts at: 1566 2327 3668 1566
Size: 761 1341 1566
Fragments arranged by size:
1566 1341 761
BclI T'GATC_A
Cuts at: 1188 1188
Size: 3668
BglI GCCn_nnn'nGGC
Cuts at: 112 584 112
Size: 472 3196
BglII A'GATC_T
Cuts at: 1183 3220 1183
Size: 2037 1631
BlpI GC'TnA_GC
Cuts at: 780 780
Size: 3668
Bme1580I G_kGCm'C
Cuts at: 1293 1386 2442 1293
Size: 93 1056 2519
Fragments arranged by size:
2519 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 880 961 1296 3440 880
Size: 81 335 2144 1108
Fragments arranged by size:
2144 1108 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 3668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1723 2215 2513 844
Size: 879 492 298 1999
Fragments arranged by size:
1999 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 130 3372 130
Size: 3242 426
BsaAI yAC'GTr
Cuts at: 1021 1021
Size: 3668
BsaBI GATnn'nnATC
Cuts at: 6 1182 3219 6
Size: 1176 2037 455
Fragments arranged by size:
2037 1176 455
BsaHI Gr'CG_yC
Cuts at: 526 1099 1347 2049 3545 526
Size: 573 248 702 1496 649
Fragments arranged by size:
1496 702 649 573 248
BsaWI w'CCGG_w
Cuts at: 629 657 857 1378 2056 2330 2477 2888 629
Size: 28 200 521 678 274 147 411 1409
Fragments arranged by size:
1409 678 521 411 274 200 147 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 416 446 416
Size: 30 3638
BseYI C'CCAG_C
Cuts at: 183 1093 1851 2428 2924 183
Size: 910 758 577 496 927
Fragments arranged by size:
927 910 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1210 1256 2464 104
Size: 1106 46 1208 1308
Fragments arranged by size:
1308 1208 1106 46
BsiHKAI G_wGCw'C
Cuts at: 254 520 682 1460 1650 2442 254
Size: 266 162 778 190 792 1480
Fragments arranged by size:
1480 792 778 266 190 162
BsmI GAATG_Cn'
Cuts at: 428 428
Size: 3668
Bsp1286I G_dGCh'C
Cuts at: 254 520 682 1293 1386 1460 1650 1712 2442
Size: 266 162 611 93 74 190 62 730
Cuts at: 2442 254
Size: 1480
Fragments arranged by size:
1480 730 611 266 190 162 93 74
62
BspEI T'CCGG_A
Cuts at: 629 2888 629
Size: 2259 1409
BspHI T'CATG_A
Cuts at: 2844 2844
Size: 3668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 518 718 932 1234 1615 518
Size: 200 214 302 381 2571
Fragments arranged by size:
2571 381 302 214 200
BsrBI CCG'CTC
Cuts at: 50 1960 2014 3498 3663 50
Size: 1910 54 1484 165 55
Fragments arranged by size:
1910 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1580 1580
Size: 3668
BsrFI r'CCGG_y
Cuts at: 534 1666 1847 2056 534
Size: 1132 181 209 2146
Fragments arranged by size:
2146 1132 209 181
BsrGI T'GTAC_A
Cuts at: 566 566
Size: 3668
BssSI C'ACGA_G
Cuts at: 1939 2297 1939
Size: 358 3310
BstAPI GCAn_nnn'nTGC
Cuts at: 1045 1045
Size: 3668
BstBI TT'CG_AA
Cuts at: 2029 2029
Size: 3668
BstYI r'GATC_y
Cuts at: 1 296 626 693 1160 1183 1518 1764 2776
Size: 295 330 67 467 23 335 246 1012
Cuts at: 2776 2862 2874 3220 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 330 295 246
86 67 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3668
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1618 1799 1962 1618
Size: 181 163 3324
Fragments arranged by size:
3324 181 163
BtsI GCAGTG_nn'
Cuts at: 911 911
Size: 3668
DraI TTT'AAA
Cuts at: 667 667
Size: 3668
DrdI GACnn_nn'nnGTC
Cuts at: 381 1374 2232 381
Size: 993 858 1817
Fragments arranged by size:
1817 993 858
EaeI y'GGCC_r
Cuts at: 474 536 1253 1427 1818 1845 3190 474
Size: 62 717 174 391 27 1345 952
Fragments arranged by size:
1345 952 717 391 174 62 27
EagI C'GGCC_G
Cuts at: 1253 1253
Size: 3668
EarI CTCTTCn'nnn_
Cuts at: 344 1691 1901 344
Size: 1347 210 2111
Fragments arranged by size:
2111 1347 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1876 2186 2332 3478 1876
Size: 310 146 1146 2066
Fragments arranged by size:
2066 1146 310 146
EcoICRI GAG'CTC
Cuts at: 680 680
Size: 3668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 1493 1925 2672 2940 326
Size: 1167 432 747 268 1054
Fragments arranged by size:
1167 1054 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 807 807
Size: 3668
EcoRI G'AATT_C
Cuts at: 672 672
Size: 3668
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 291 1687 291
Size: 1396 2272
FspI TGC'GCA
Cuts at: 1449 1449
Size: 3668
HaeII r_GCGC'y
Cuts at: 1102 1350 2372 3351 1102
Size: 248 1022 979 1419
Fragments arranged by size:
1419 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 416 448 618 650 1510 1540 1542 1572 416
Size: 32 170 32 860 30 2 30 2512
Fragments arranged by size:
2512 860 170 32 32 30 30 2
HincII GTy'rAC
Cuts at: 707 707
Size: 3668
KasI G'GCGC_C
Cuts at: 1098 1346 1098
Size: 248 3420
KpnI G_GTAC'C
Cuts at: 688 688
Size: 3668
MluI A'CGCG_T
Cuts at: 3101 3101
Size: 3668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2523 2339
Size: 184 3484
MscI TGG'CCA
Cuts at: 476 1429 476
Size: 953 2715
MslI CAynn'nnrTG
Cuts at: 1657 1784 1657
Size: 127 3541
MspA1I CmG'CkG
Cuts at: 118 779 1093 1453 2466 2711 118
Size: 661 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 661 360 314 245
NaeI GCC'GGC
Cuts at: 536 1849 536
Size: 1313 2355
NarI GG'CG_CC
Cuts at: 1099 1347 1099
Size: 248 3420
NcoI C'CATG_G
Cuts at: 107 107
Size: 3668
NgoMIV G'CCGG_C
Cuts at: 534 1847 534
Size: 1313 2355
NspI r_CATG'y
Cuts at: 721 1221 2128 721
Size: 500 907 2261
Fragments arranged by size:
2261 907 500
PciI A'CATG_T
Cuts at: 2124 2124
Size: 3668
PfoI T'CCnGG_A
Cuts at: 434 434
Size: 3668
PmeI GTTT'AAAC
Cuts at: 667 667
Size: 3668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 3636
PstI C_TGCA'G
Cuts at: 123 515 715 1400 123
Size: 392 200 685 2391
Fragments arranged by size:
2391 685 392 200
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3668
PvuII CAG'CTG
Cuts at: 1093 1453 1093
Size: 360 3308
RsrII CG'GwC_CG
Cuts at: 1863 1863
Size: 3668
SacI G_AGCT'C
Cuts at: 682 682
Size: 3668
SalI G'TCGA_C
Cuts at: 705 705
Size: 3668
SapI GCTCTTCn'nnn_
Cuts at: 1691 1901 1691
Size: 210 3458
SbfI CC_TGCA'GG
Cuts at: 715 715
Size: 3668
ScaI AGT'ACT
Cuts at: 3185 3185
Size: 3668
SfcI C'TryA_G
Cuts at: 33 119 511 711 1396 2389 2580 33
Size: 86 392 200 685 993 191 1121
Fragments arranged by size:
1121 993 685 392 200 191 86
SfoI GGC'GCC
Cuts at: 1100 1348 1100
Size: 248 3420
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
SmaI CCC'GGG
Cuts at: 690 690
Size: 3668
SmlI C'TyrA_G
Cuts at: 823 1738 2230 2492 823
Size: 915 492 262 1999
Fragments arranged by size:
1999 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3668
SphI G_CATG'C
Cuts at: 721 1221 721
Size: 500 3168
StuI AGG'CCT
Cuts at: 334 334
Size: 3668
StyI C'CwwG_G
Cuts at: 107 802 1156 107
Size: 695 354 2619
Fragments arranged by size:
2619 695 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 520 1251 1384 1843 2052 520
Size: 731 133 459 209 2136
Fragments arranged by size:
2136 731 459 209 133
TatI w'GTAC_w
Cuts at: 566 3183 566
Size: 2617 1051
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 3636
Tth111I GACn'n_nGTC
Cuts at: 1465 1465
Size: 3668
XbaI T'CTAG_A
Cuts at: 699 699
Size: 3668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1163 1163
Size: 3668
XmaI C'CCGG_G
Cuts at: 688 688
Size: 3668
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BseRI BsgI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI