Restriction map for
pF1KB9786
(Circular) (Six-base) MAPSORT of: pF1KB9786.seq Check: 7091 from: 1 to: 3686
pF1KB9786 3686 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 724 724
Size: 3686
Acc65I G'GTAC_C
Cuts at: 702 702
Size: 3686
AclI AA'CG_TT
Cuts at: 335 3526 335
Size: 3191 495
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1511 1943 2690 2958 1511
Size: 432 747 268 2239
Fragments arranged by size:
2239 747 432 268
AflIII A'CryG_T
Cuts at: 1038 2142 3119 1038
Size: 1104 977 1605
Fragments arranged by size:
1605 1104 977
AgeI A'CCGG_T
Cuts at: 2074 2074
Size: 3686
AlwNI CAG_nnn'CTG
Cuts at: 540 991 2558 540
Size: 451 1567 1668
Fragments arranged by size:
1668 1567 451
ApaLI G'TGCA_C
Cuts at: 2456 2456
Size: 3686
ApoI r'AATT_y
Cuts at: 690 3039 3295 690
Size: 2349 256 1081
Fragments arranged by size:
2349 1081 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3686
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
AvaI C'yCGr_G
Cuts at: 706 706
Size: 3686
BamHI G'GATC_C
Cuts at: 711 711
Size: 3686
BanI G'GyrC_C
Cuts at: 448 488 510 702 1116 1364 1399 448
Size: 40 22 192 414 248 35 2735
Fragments arranged by size:
2735 414 248 192 40 35 22
BanII G_rGCy'C
Cuts at: 471 700 1730 471
Size: 229 1030 2427
Fragments arranged by size:
2427 1030 229
BbeI G_GCGC'C
Cuts at: 492 514 1120 1368 492
Size: 22 606 248 2810
Fragments arranged by size:
2810 606 248 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3652
BciVI GTATCCnnnnn_n'
Cuts at: 1584 2345 3686 1584
Size: 761 1341 1584
Fragments arranged by size:
1584 1341 761
BclI T'GATC_A
Cuts at: 1206 1206
Size: 3686
BglII A'GATC_T
Cuts at: 1201 3238 1201
Size: 2037 1649
BlpI GC'TnA_GC
Cuts at: 579 798 579
Size: 219 3467
Bme1580I G_kGCm'C
Cuts at: 434 451 1311 1404 2460 434
Size: 17 860 93 1056 1660
Fragments arranged by size:
1660 1056 860 93 17
BmgBI CAC'GTC
Cuts at: 158 158
Size: 3686
BmrI ACTGGGnnnn_n'
Cuts at: 898 979 1314 3458 898
Size: 81 335 2144 1126
Fragments arranged by size:
2144 1126 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 3686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 862 1741 2233 2531 478
Size: 384 879 492 298 1633
Fragments arranged by size:
1633 879 492 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 3390 3390
Size: 3686
BsaAI yAC'GTr
Cuts at: 1039 1039
Size: 3686
BsaBI GATnn'nnATC
Cuts at: 6 1200 3237 6
Size: 1194 2037 455
Fragments arranged by size:
2037 1194 455
BsaHI Gr'CG_yC
Cuts at: 489 511 1117 1365 2067 3563 489
Size: 22 606 248 702 1496 612
Fragments arranged by size:
1496 702 612 606 248 22
BsaWI w'CCGG_w
Cuts at: 875 1396 2074 2348 2495 2906 875
Size: 521 678 274 147 411 1655
Fragments arranged by size:
1655 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 307
Size: 3686
BseYI C'CCAG_C
Cuts at: 470 1111 1869 2446 2942 470
Size: 641 758 577 496 1214
Fragments arranged by size:
1214 758 641 577 496
BsiEI CG_ry'CG
Cuts at: 104 1228 1274 2482 104
Size: 1124 46 1208 1308
Fragments arranged by size:
1308 1208 1124 46
BsiHKAI G_wGCw'C
Cuts at: 638 700 1478 1668 2460 638
Size: 62 778 190 792 1864
Fragments arranged by size:
1864 792 778 190 62
BsmI GAATG_Cn'
Cuts at: 580 580
Size: 3686
Bsp1286I G_dGCh'C
Cuts at: 434 451 471 638 700 1311 1404 1478 1668
Size: 17 20 167 62 611 93 74 190
Cuts at: 1668 1730 2460 434
Size: 62 730 1660
Fragments arranged by size:
1660 730 611 190 167 93 74 62
62 20 17
BspEI T'CCGG_A
Cuts at: 2906 2906
Size: 3686
BspHI T'CATG_A
Cuts at: 2862 2862
Size: 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 736 950 1252 1633 736
Size: 214 302 381 2789
Fragments arranged by size:
2789 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1978 2032 3516 3681 50
Size: 1928 54 1484 165 55
Fragments arranged by size:
1928 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 356 1598 356
Size: 1242 2444
BsrFI r'CCGG_y
Cuts at: 1684 1865 2074 1684
Size: 181 209 3296
Fragments arranged by size:
3296 209 181
BssHII G'CGCG_C
Cuts at: 230 230
Size: 3686
BssSI C'ACGA_G
Cuts at: 1957 2315 1957
Size: 358 3328
BstAPI GCAn_nnn'nTGC
Cuts at: 122 158 1063 122
Size: 36 905 2745
Fragments arranged by size:
2745 905 36
BstBI TT'CG_AA
Cuts at: 2047 2047
Size: 3686
BstXI CCAn_nnnn'nTGG
Cuts at: 441 441
Size: 3686
BstYI r'GATC_y
Cuts at: 1 551 711 1178 1201 1536 1782 2794 2880
Size: 550 160 467 23 335 246 1012 86
Cuts at: 2880 2892 3238 1
Size: 12 346 449
Fragments arranged by size:
1012 550 467 449 346 335 246 160
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1636 1817 1980 1636
Size: 181 163 3342
Fragments arranged by size:
3342 181 163
BtsI GCAGTG_nn'
Cuts at: 278 417 929 278
Size: 139 512 3035
Fragments arranged by size:
3035 512 139
DraI TTT'AAA
Cuts at: 685 685
Size: 3686
DraIII CAC_nnn'GTG
Cuts at: 561 561
Size: 3686
DrdI GACnn_nn'nnGTC
Cuts at: 1392 2250 1392
Size: 858 2828
EaeI y'GGCC_r
Cuts at: 542 1271 1445 1836 1863 3208 542
Size: 729 174 391 27 1345 1020
Fragments arranged by size:
1345 1020 729 391 174 27
EagI C'GGCC_G
Cuts at: 1271 1271
Size: 3686
EarI CTCTTCn'nnn_
Cuts at: 1709 1919 1709
Size: 210 3476
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 356 1894 2204 2350 3496 356
Size: 1538 310 146 1146 546
Fragments arranged by size:
1538 1146 546 310 146
EcoICRI GAG'CTC
Cuts at: 698 698
Size: 3686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1511 1943 2690 2958 1511
Size: 432 747 268 2239
Fragments arranged by size:
2239 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 589 589
Size: 3686
EcoO109I rG'GnC_Cy
Cuts at: 459 825 459
Size: 366 3320
EcoRI G'AATT_C
Cuts at: 690 690
Size: 3686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1705 1705
Size: 3686
FspI TGC'GCA
Cuts at: 1467 1467
Size: 3686
HaeII r_GCGC'y
Cuts at: 492 514 1120 1368 2390 3369 492
Size: 22 606 248 1022 979 809
Fragments arranged by size:
1022 979 809 606 248 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1528 1558 1560 1590 1528
Size: 30 2 30 3624
Fragments arranged by size:
3624 30 30 2
HincII GTy'rAC
Cuts at: 246 725 246
Size: 479 3207
KasI G'GCGC_C
Cuts at: 488 510 1116 1364 488
Size: 22 606 248 2810
Fragments arranged by size:
2810 606 248 22
KpnI G_GTAC'C
Cuts at: 706 706
Size: 3686
MluI A'CGCG_T
Cuts at: 3119 3119
Size: 3686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2541 2357
Size: 184 3502
MscI TGG'CCA
Cuts at: 544 1447 544
Size: 903 2783
MslI CAynn'nnrTG
Cuts at: 1675 1802 1675
Size: 127 3559
MspA1I CmG'CkG
Cuts at: 797 1111 1471 2484 2729 797
Size: 314 360 1013 245 1754
Fragments arranged by size:
1754 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1867 1867
Size: 3686
NarI GG'CG_CC
Cuts at: 489 511 1117 1365 489
Size: 22 606 248 2810
Fragments arranged by size:
2810 606 248 22
NgoMIV G'CCGG_C
Cuts at: 1865 1865
Size: 3686
NspI r_CATG'y
Cuts at: 739 1239 2146 739
Size: 500 907 2279
Fragments arranged by size:
2279 907 500
PciI A'CATG_T
Cuts at: 2142 2142
Size: 3686
PflMI CCAn_nnn'nTGG
Cuts at: 540 540
Size: 3686
PmeI GTTT'AAAC
Cuts at: 685 685
Size: 3686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 3654
PpuMI rG'GwC_Cy
Cuts at: 459 459
Size: 3686
PstI C_TGCA'G
Cuts at: 733 1418 733
Size: 685 3001
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3686
PvuII CAG'CTG
Cuts at: 1111 1471 1111
Size: 360 3326
RsrII CG'GwC_CG
Cuts at: 1881 1881
Size: 3686
SacI G_AGCT'C
Cuts at: 700 700
Size: 3686
SalI G'TCGA_C
Cuts at: 723 723
Size: 3686
SapI GCTCTTCn'nnn_
Cuts at: 1709 1919 1709
Size: 210 3476
SbfI CC_TGCA'GG
Cuts at: 733 733
Size: 3686
ScaI AGT'ACT
Cuts at: 3203 3203
Size: 3686
SfcI C'TryA_G
Cuts at: 33 729 1414 2407 2598 33
Size: 696 685 993 191 1121
Fragments arranged by size:
1121 993 696 685 191
SfoI GGC'GCC
Cuts at: 490 512 1118 1366 490
Size: 22 606 248 2810
Fragments arranged by size:
2810 606 248 22
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
SmaI CCC'GGG
Cuts at: 708 708
Size: 3686
SmlI C'TyrA_G
Cuts at: 493 841 1756 2248 2510 493
Size: 348 915 492 262 1669
Fragments arranged by size:
1669 915 492 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3686
SphI G_CATG'C
Cuts at: 739 1239 739
Size: 500 3186
StyI C'CwwG_G
Cuts at: 820 1174 820
Size: 354 3332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 124 1269 1402 1861 2070 124
Size: 1145 133 459 209 1740
Fragments arranged by size:
1740 1145 459 209 133
TatI w'GTAC_w
Cuts at: 200 3201 200
Size: 3001 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 3654
Tth111I GACn'n_nGTC
Cuts at: 1483 1483
Size: 3686
XbaI T'CTAG_A
Cuts at: 717 717
Size: 3686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 441 1181 441
Size: 740 2946
XmaI C'CCGG_G
Cuts at: 706 706
Size: 3686
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI
BstEII BstZ17I Bsu36I BtgI ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI