Restriction map for
pF1KB9784
(Circular) (Six-base) MAPSORT of: pF1KB9784.seq Check: 9767 from: 1 to: 3680
pF1KB9784 3680 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 570 638 570
Size: 68 3612
AccI GT'mk_AC
Cuts at: 718 718
Size: 3680
Acc65I G'GTAC_C
Cuts at: 696 696
Size: 3680
AclI AA'CG_TT
Cuts at: 3520 3520
Size: 3680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1505 1937 2684 2952 1505
Size: 432 747 268 2233
Fragments arranged by size:
2233 747 432 268
AflIII A'CryG_T
Cuts at: 1032 2136 3113 1032
Size: 1104 977 1599
Fragments arranged by size:
1599 1104 977
AgeI A'CCGG_T
Cuts at: 2068 2068
Size: 3680
AlwNI CAG_nnn'CTG
Cuts at: 399 985 2552 399
Size: 586 1567 1527
Fragments arranged by size:
1567 1527 586
ApaI G_GGCC'C
Cuts at: 144 305 547 144
Size: 161 242 3277
Fragments arranged by size:
3277 242 161
ApaLI G'TGCA_C
Cuts at: 2450 2450
Size: 3680
ApoI r'AATT_y
Cuts at: 684 3033 3289 684
Size: 2349 256 1075
Fragments arranged by size:
2349 1075 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3680
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
AvaI C'yCGr_G
Cuts at: 169 204 245 700 169
Size: 35 41 455 3149
Fragments arranged by size:
3149 455 41 35
BamHI G'GATC_C
Cuts at: 705 705
Size: 3680
BanI G'GyrC_C
Cuts at: 134 670 696 1110 1358 1393 134
Size: 536 26 414 248 35 2421
Fragments arranged by size:
2421 536 414 248 35 26
BanII G_rGCy'C
Cuts at: 144 264 305 547 694 1724 144
Size: 120 41 242 147 1030 2100
Fragments arranged by size:
2100 1030 242 147 120 41
BbeI G_GCGC'C
Cuts at: 138 1114 1362 138
Size: 976 248 2456
Fragments arranged by size:
2456 976 248
BbsI GAAGACnn'nnnn_
Cuts at: 118 118
Size: 3680
BciVI GTATCCnnnnn_n'
Cuts at: 1578 2339 3680 1578
Size: 761 1341 1578
Fragments arranged by size:
1578 1341 761
BclI T'GATC_A
Cuts at: 1200 1200
Size: 3680
BglI GCCn_nnn'nGGC
Cuts at: 132 394 527 610 132
Size: 262 133 83 3202
Fragments arranged by size:
3202 262 133 83
BglII A'GATC_T
Cuts at: 1195 3232 1195
Size: 2037 1643
BlpI GC'TnA_GC
Cuts at: 792 792
Size: 3680
Bme1580I G_kGCm'C
Cuts at: 144 305 547 673 1305 1398 2454 144
Size: 161 242 126 632 93 1056 1370
Fragments arranged by size:
1370 1056 632 242 161 126 93
BmrI ACTGGGnnnn_n'
Cuts at: 892 973 1308 3452 892
Size: 81 335 2144 1120
Fragments arranged by size:
2144 1120 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 3680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 553 361
Size: 192 3488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1735 2227 2525 856
Size: 879 492 298 2011
Fragments arranged by size:
2011 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3384 3384
Size: 3680
BsaAI yAC'GTr
Cuts at: 420 1033 420
Size: 613 3067
BsaBI GATnn'nnATC
Cuts at: 6 1194 3231 6
Size: 1188 2037 455
Fragments arranged by size:
2037 1188 455
BsaHI Gr'CG_yC
Cuts at: 135 181 1111 1359 2061 3557 135
Size: 46 930 248 702 1496 258
Fragments arranged by size:
1496 930 702 258 248 46
BsaWI w'CCGG_w
Cuts at: 869 1390 2068 2342 2489 2900 869
Size: 521 678 274 147 411 1649
Fragments arranged by size:
1649 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 586 614 1105 1863 2440 2936 586
Size: 28 491 758 577 496 1330
Fragments arranged by size:
1330 758 577 496 491 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 169
Size: 3680
BsiEI CG_ry'CG
Cuts at: 104 241 1222 1268 2476 104
Size: 137 981 46 1208 1308
Fragments arranged by size:
1308 1208 981 137 46
BsiHKAI G_wGCw'C
Cuts at: 694 1472 1662 2454 694
Size: 778 190 792 1920
Fragments arranged by size:
1920 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 445 445
Size: 3680
Bsp1286I G_dGCh'C
Cuts at: 144 264 305 547 673 694 1305 1398 1472
Size: 120 41 242 126 21 611 93 74
Cuts at: 1472 1662 1724 2454 144
Size: 190 62 730 1370
Fragments arranged by size:
1370 730 611 242 190 126 120 93
74 62 41 21
BspEI T'CCGG_A
Cuts at: 2900 2900
Size: 3680
BspHI T'CATG_A
Cuts at: 2856 2856
Size: 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 570 638 730 944 1246 1627 570
Size: 68 92 214 302 381 2623
Fragments arranged by size:
2623 381 302 214 92 68
BsrBI CCG'CTC
Cuts at: 50 360 1972 2026 3510 3675 50
Size: 310 1612 54 1484 165 55
Fragments arranged by size:
1612 1484 310 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1592 1592
Size: 3680
BsrFI r'CCGG_y
Cuts at: 122 216 236 240 1678 1859 2068 122
Size: 94 20 4 1438 181 209 1734
Fragments arranged by size:
1734 1438 209 181 94 20 4
BssHII G'CGCG_C
Cuts at: 423 423
Size: 3680
BssSI C'ACGA_G
Cuts at: 1951 2309 1951
Size: 358 3322
BstAPI GCAn_nnn'nTGC
Cuts at: 369 1057 369
Size: 688 2992
BstBI TT'CG_AA
Cuts at: 2041 2041
Size: 3680
BstYI r'GATC_y
Cuts at: 1 650 705 1172 1195 1530 1776 2788 2874
Size: 649 55 467 23 335 246 1012 86
Cuts at: 2874 2886 3232 1
Size: 12 346 449
Fragments arranged by size:
1012 649 467 449 346 335 246 86
55 23 12
BtgI C'CryG_G
Cuts at: 130 605 130
Size: 475 3205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1630 1811 1974 1630
Size: 181 163 3336
Fragments arranged by size:
3336 181 163
BtsI GCAGTG_nn'
Cuts at: 923 923
Size: 3680
DraI TTT'AAA
Cuts at: 679 679
Size: 3680
DrdI GACnn_nn'nnGTC
Cuts at: 1386 2244 1386
Size: 858 2822
EaeI y'GGCC_r
Cuts at: 218 238 286 309 464 491 1265 1439 1830
Size: 20 48 23 155 27 774 174 391
Cuts at: 1830 1857 3202 218
Size: 27 1345 696
Fragments arranged by size:
1345 774 696 391 174 155 48 27
27 23 20
EagI C'GGCC_G
Cuts at: 238 1265 238
Size: 1027 2653
EarI CTCTTCn'nnn_
Cuts at: 1703 1913 1703
Size: 210 3470
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1888 2198 2344 3490 1888
Size: 310 146 1146 2078
Fragments arranged by size:
2078 1146 310 146
EcoICRI GAG'CTC
Cuts at: 692 692
Size: 3680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 553 1505 1937 2684 2952 361
Size: 192 952 432 747 268 1089
Fragments arranged by size:
1089 952 747 432 268 192
EcoO109I rG'GnC_Cy
Cuts at: 141 191 292 396 544 819 141
Size: 50 101 104 148 275 3002
Fragments arranged by size:
3002 275 148 104 101 50
EcoRI G'AATT_C
Cuts at: 684 684
Size: 3680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1699 1699
Size: 3680
FseI GG_CCGG'CC
Cuts at: 244 244
Size: 3680
FspI TGC'GCA
Cuts at: 1461 1461
Size: 3680
HaeII r_GCGC'y
Cuts at: 138 1114 1362 2384 3363 138
Size: 976 248 1022 979 455
Fragments arranged by size:
1022 979 976 455 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1522 1552 1554 1584 1522
Size: 30 2 30 3618
Fragments arranged by size:
3618 30 30 2
HincII GTy'rAC
Cuts at: 719 719
Size: 3680
KasI G'GCGC_C
Cuts at: 134 1110 1358 134
Size: 976 248 2456
Fragments arranged by size:
2456 976 248
KpnI G_GTAC'C
Cuts at: 700 700
Size: 3680
MluI A'CGCG_T
Cuts at: 3113 3113
Size: 3680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2351 2535 2351
Size: 184 3496
MscI TGG'CCA
Cuts at: 466 1441 466
Size: 975 2705
MslI CAynn'nnrTG
Cuts at: 1669 1796 1669
Size: 127 3553
MspA1I CmG'CkG
Cuts at: 333 366 791 1105 1465 2478 2723 333
Size: 33 425 314 360 1013 245 1290
Fragments arranged by size:
1290 1013 425 360 314 245 33
NaeI GCC'GGC
Cuts at: 218 238 242 1861 218
Size: 20 4 1619 2037
Fragments arranged by size:
2037 1619 20 4
NarI GG'CG_CC
Cuts at: 135 1111 1359 135
Size: 976 248 2456
Fragments arranged by size:
2456 976 248
NgoMIV G'CCGG_C
Cuts at: 216 236 240 1859 216
Size: 20 4 1619 2037
Fragments arranged by size:
2037 1619 20 4
NspI r_CATG'y
Cuts at: 733 1233 2140 733
Size: 500 907 2273
Fragments arranged by size:
2273 907 500
PasI CC'CwG_GG
Cuts at: 621 621
Size: 3680
PciI A'CATG_T
Cuts at: 2136 2136
Size: 3680
PmeI GTTT'AAAC
Cuts at: 679 679
Size: 3680
PmlI CAC'GTG
Cuts at: 420 420
Size: 3680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 2819
Size: 32 3648
PpuMI rG'GwC_Cy
Cuts at: 191 292 191
Size: 101 3579
PspOMI G'GGCC_C
Cuts at: 140 301 543 140
Size: 161 242 3277
Fragments arranged by size:
3277 242 161
PstI C_TGCA'G
Cuts at: 635 727 1412 635
Size: 92 685 2903
Fragments arranged by size:
2903 685 92
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3680
PvuII CAG'CTG
Cuts at: 1105 1465 1105
Size: 360 3320
RsrII CG'GwC_CG
Cuts at: 1875 1875
Size: 3680
SacI G_AGCT'C
Cuts at: 694 694
Size: 3680
SalI G'TCGA_C
Cuts at: 717 717
Size: 3680
SanDI GG'GwC_CC
Cuts at: 191 191
Size: 3680
SapI GCTCTTCn'nnn_
Cuts at: 1703 1913 1703
Size: 210 3470
SbfI CC_TGCA'GG
Cuts at: 727 727
Size: 3680
ScaI AGT'ACT
Cuts at: 3197 3197
Size: 3680
SfcI C'TryA_G
Cuts at: 33 631 723 1408 2401 2592 33
Size: 598 92 685 993 191 1121
Fragments arranged by size:
1121 993 685 598 191 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 610 610
Size: 3680
SfoI GGC'GCC
Cuts at: 136 1112 1360 136
Size: 976 248 2456
Fragments arranged by size:
2456 976 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
SmaI CCC'GGG
Cuts at: 171 206 247 702 171
Size: 35 41 455 3149
Fragments arranged by size:
3149 455 41 35
SmlI C'TyrA_G
Cuts at: 835 1750 2242 2504 835
Size: 915 492 262 2011
Fragments arranged by size:
2011 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3680
SphI G_CATG'C
Cuts at: 733 1233 733
Size: 500 3180
SrfI GCCC'GGGC
Cuts at: 247 247
Size: 3680
StuI AGG'CCT
Cuts at: 530 530
Size: 3680
StyI C'CwwG_G
Cuts at: 221 814 1168 221
Size: 593 354 2733
Fragments arranged by size:
2733 593 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1263 1396 1855 2064 1263
Size: 133 459 209 2879
Fragments arranged by size:
2879 459 209 133
TatI w'GTAC_w
Cuts at: 3195 3195
Size: 3680
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 3648
Tth111I GACn'n_nGTC
Cuts at: 507 1477 507
Size: 970 2710
XbaI T'CTAG_A
Cuts at: 711 711
Size: 3680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1175 1175
Size: 3680
XmaI C'CCGG_G
Cuts at: 169 204 245 700 169
Size: 35 41 455 3149
Fragments arranged by size:
3149 455 41 35
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PshAI PsiI PspXI PsrI SacII
SexAI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI