Restriction map for
pF1KB9783
(Circular) (Six-base) MAPSORT of: pF1KB9783.seq Check: 1076 from: 1 to: 3866
pF1KB9783 3866 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 123 753 123
Size: 630 3236
AatII G_ACGT'C
Cuts at: 653 653
Size: 3866
AccI GT'mk_AC
Cuts at: 904 904
Size: 3866
Acc65I G'GTAC_C
Cuts at: 882 882
Size: 3866
AclI AA'CG_TT
Cuts at: 3706 3706
Size: 3866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 716 1691 2123 2870 3138 716
Size: 975 432 747 268 1444
Fragments arranged by size:
1444 975 747 432 268
AflIII A'CryG_T
Cuts at: 1218 2322 3299 1218
Size: 1104 977 1785
Fragments arranged by size:
1785 1104 977
AgeI A'CCGG_T
Cuts at: 2254 2254
Size: 3866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 388 388
Size: 3866
AlwNI CAG_nnn'CTG
Cuts at: 357 1171 2738 357
Size: 814 1567 1485
Fragments arranged by size:
1567 1485 814
ApaI G_GGCC'C
Cuts at: 496 590 731 496
Size: 94 141 3631
Fragments arranged by size:
3631 141 94
ApaLI G'TGCA_C
Cuts at: 2636 2636
Size: 3866
ApoI r'AATT_y
Cuts at: 870 3219 3475 870
Size: 2349 256 1261
Fragments arranged by size:
2349 1261 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3866
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
AvaI C'yCGr_G
Cuts at: 886 886
Size: 3866
BamHI G'GATC_C
Cuts at: 891 891
Size: 3866
BanI G'GyrC_C
Cuts at: 471 501 882 1296 1544 1579 471
Size: 30 381 414 248 35 2758
Fragments arranged by size:
2758 414 381 248 35 30
BanII G_rGCy'C
Cuts at: 382 448 496 590 623 731 880 1910 382
Size: 66 48 94 33 108 149 1030 2338
Fragments arranged by size:
2338 1030 149 108 94 66 48 33
BbeI G_GCGC'C
Cuts at: 1300 1548 1300
Size: 248 3618
BbvCI CC'TCA_GC
Cuts at: 827 827
Size: 3866
BciVI GTATCCnnnnn_n'
Cuts at: 1764 2525 3866 1764
Size: 761 1341 1764
Fragments arranged by size:
1764 1341 761
BclI T'GATC_A
Cuts at: 1386 1386
Size: 3866
BglI GCCn_nnn'nGGC
Cuts at: 434 585 718 434
Size: 151 133 3582
Fragments arranged by size:
3582 151 133
BglII A'GATC_T
Cuts at: 1381 3418 1381
Size: 2037 1829
BlpI GC'TnA_GC
Cuts at: 978 978
Size: 3866
Bme1580I G_kGCm'C
Cuts at: 430 474 496 506 590 731 1491 1584 2640
Size: 44 22 10 84 141 760 93 1056
Cuts at: 2640 430
Size: 1656
Fragments arranged by size:
1656 1056 760 141 93 84 44 22
10
BmgBI CAC'GTC
Cuts at: 425 425
Size: 3866
BmrI ACTGGGnnnn_n'
Cuts at: 1078 1159 1494 3638 1078
Size: 81 335 2144 1306
Fragments arranged by size:
2144 1306 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 3866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 616
Size: 3866
Bpu10I CC'TnA_GC
Cuts at: 476 827 476
Size: 351 3515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 741 1042 1921 2413 2711 741
Size: 301 879 492 298 1896
Fragments arranged by size:
1896 879 492 301 298
BsaI GGTCTCn'nnnn_
Cuts at: 290 836 3570 290
Size: 546 2734 586
Fragments arranged by size:
2734 586 546
BsaAI yAC'GTr
Cuts at: 230 1219 230
Size: 989 2877
BsaBI GATnn'nnATC
Cuts at: 6 1380 3417 6
Size: 1374 2037 455
Fragments arranged by size:
2037 1374 455
BsaHI Gr'CG_yC
Cuts at: 650 1297 1545 2247 3743 650
Size: 647 248 702 1496 773
Fragments arranged by size:
1496 773 702 647 248
BsaWI w'CCGG_w
Cuts at: 155 1055 1576 2254 2528 2675 3086 155
Size: 900 521 678 274 147 411 935
Fragments arranged by size:
935 900 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 734 764 734
Size: 30 3836
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 295 304 715 295
Size: 9 411 3446
Fragments arranged by size:
3446 411 9
BseYI C'CCAG_C
Cuts at: 1291 2049 2626 3122 1291
Size: 758 577 496 2035
Fragments arranged by size:
2035 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1408 1454 2662 104
Size: 1304 46 1208 1308
Fragments arranged by size:
1308 1304 1208 46
BsiHKAI G_wGCw'C
Cuts at: 248 382 623 880 1658 1848 2640 248
Size: 134 241 257 778 190 792 1474
Fragments arranged by size:
1474 792 778 257 241 190 134
Bsp1286I G_dGCh'C
Cuts at: 248 382 430 448 474 496 506 590 623
Size: 134 48 18 26 22 10 84 33
Cuts at: 623 731 880 1491 1584 1658 1848 1910 2640
Size: 108 149 611 93 74 190 62 730
Cuts at: 2640 248
Size: 1474
Fragments arranged by size:
1474 730 611 190 149 134 108 93
84 74 62 48 33 26 22 18
10
BspEI T'CCGG_A
Cuts at: 155 3086 155
Size: 2931 935
BspHI T'CATG_A
Cuts at: 3042 3042
Size: 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 123 380 560 753 916 1130 1432 1813 123
Size: 257 180 193 163 214 302 381 2176
Fragments arranged by size:
2176 381 302 257 214 193 180 163
BsrBI CCG'CTC
Cuts at: 50 522 2158 2212 3696 3861 50
Size: 472 1636 54 1484 165 55
Fragments arranged by size:
1636 1484 472 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1778 1778
Size: 3866
BsrFI r'CCGG_y
Cuts at: 639 1864 2045 2254 639
Size: 1225 181 209 2251
Fragments arranged by size:
2251 1225 209 181
BsrGI T'GTAC_A
Cuts at: 359 359
Size: 3866
BssSI C'ACGA_G
Cuts at: 2137 2495 2137
Size: 358 3508
BstAPI GCAn_nnn'nTGC
Cuts at: 1243 1243
Size: 3866
BstBI TT'CG_AA
Cuts at: 2227 2227
Size: 3866
BstEII G'GTnAC_C
Cuts at: 765 765
Size: 3866
BstYI r'GATC_y
Cuts at: 1 891 1358 1381 1716 1962 2974 3060 3072
Size: 890 467 23 335 246 1012 86 12
Cuts at: 3072 3418 1
Size: 346 449
Fragments arranged by size:
1012 890 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 449 449
Size: 3866
BtgI C'CryG_G
Cuts at: 107 143 582 107
Size: 36 439 3391
Fragments arranged by size:
3391 439 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1816 1997 2160 1816
Size: 181 163 3522
Fragments arranged by size:
3522 181 163
BtsI GCAGTG_nn'
Cuts at: 855 1109 855
Size: 254 3612
DraI TTT'AAA
Cuts at: 865 865
Size: 3866
DrdI GACnn_nn'nnGTC
Cuts at: 1572 2430 1572
Size: 858 3008
EaeI y'GGCC_r
Cuts at: 719 1451 1625 2016 2043 3388 719
Size: 732 174 391 27 1345 1197
Fragments arranged by size:
1345 1197 732 391 174 27
EagI C'GGCC_G
Cuts at: 1451 1451
Size: 3866
EarI CTCTTCn'nnn_
Cuts at: 236 313 628 692 1889 2099 236
Size: 77 315 64 1197 210 2003
Fragments arranged by size:
2003 1197 315 210 77 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2074 2384 2530 3676 2074
Size: 310 146 1146 2264
Fragments arranged by size:
2264 1146 310 146
EcoICRI GAG'CTC
Cuts at: 380 621 878 380
Size: 241 257 3368
Fragments arranged by size:
3368 257 241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 716 1691 2123 2870 3138 616
Size: 100 975 432 747 268 1344
Fragments arranged by size:
1344 975 747 432 268 100
EcoNI CCTnn'n_nnAGG
Cuts at: 721 721
Size: 3866
EcoO109I rG'GnC_Cy
Cuts at: 492 493 727 728 1005 492
Size: 1 234 1 277 3353
Fragments arranged by size:
3353 277 234 1 1
EcoRI G'AATT_C
Cuts at: 870 870
Size: 3866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 429 513 1885 429
Size: 84 1372 2410
Fragments arranged by size:
2410 1372 84
FspI TGC'GCA
Cuts at: 686 1647 686
Size: 961 2905
HaeII r_GCGC'y
Cuts at: 1300 1548 2570 3549 1300
Size: 248 1022 979 1617
Fragments arranged by size:
1617 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 605 637 1708 1738 1740 1770 289
Size: 32 284 32 1071 30 2 30 2385
Fragments arranged by size:
2385 1071 284 32 32 30 30 2
HincII GTy'rAC
Cuts at: 905 905
Size: 3866
KasI G'GCGC_C
Cuts at: 1296 1544 1296
Size: 248 3618
KpnI G_GTAC'C
Cuts at: 886 886
Size: 3866
MluI A'CGCG_T
Cuts at: 3299 3299
Size: 3866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2537 2721 2537
Size: 184 3682
MscI TGG'CCA
Cuts at: 1627 1627
Size: 3866
MslI CAynn'nnrTG
Cuts at: 1855 1982 1855
Size: 127 3739
MspA1I CmG'CkG
Cuts at: 354 440 584 826 977 1291 1651 2664 2909
Size: 86 144 242 151 314 360 1013 245
Cuts at: 2909 354
Size: 1311
Fragments arranged by size:
1311 1013 360 314 245 242 151 144
86
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 3866
NarI GG'CG_CC
Cuts at: 1297 1545 1297
Size: 248 3618
NcoI C'CATG_G
Cuts at: 107 107
Size: 3866
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 3866
NspI r_CATG'y
Cuts at: 919 1419 2326 919
Size: 500 907 2459
Fragments arranged by size:
2459 907 500
PciI A'CATG_T
Cuts at: 2322 2322
Size: 3866
PmeI GTTT'AAAC
Cuts at: 865 865
Size: 3866
PmlI CAC'GTG
Cuts at: 230 230
Size: 3866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 121 153 3005 3037 121
Size: 32 2852 32 950
Fragments arranged by size:
2852 950 32 32
PspOMI G'GGCC_C
Cuts at: 492 586 727 492
Size: 94 141 3631
Fragments arranged by size:
3631 141 94
PstI C_TGCA'G
Cuts at: 353 632 659 913 1598 353
Size: 279 27 254 685 2621
Fragments arranged by size:
2621 685 279 254 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3866
PvuII CAG'CTG
Cuts at: 826 1291 1651 826
Size: 465 360 3041
Fragments arranged by size:
3041 465 360
RsrII CG'GwC_CG
Cuts at: 2061 2061
Size: 3866
SacI G_AGCT'C
Cuts at: 382 623 880 382
Size: 241 257 3368
Fragments arranged by size:
3368 257 241
SacII CC_GC'GG
Cuts at: 585 585
Size: 3866
SalI G'TCGA_C
Cuts at: 903 903
Size: 3866
SapI GCTCTTCn'nnn_
Cuts at: 236 628 1889 2099 236
Size: 392 1261 210 2003
Fragments arranged by size:
2003 1261 392 210
SbfI CC_TGCA'GG
Cuts at: 632 659 913 632
Size: 27 254 3585
Fragments arranged by size:
3585 254 27
ScaI AGT'ACT
Cuts at: 3383 3383
Size: 3866
SfcI C'TryA_G
Cuts at: 33 349 628 655 909 1594 2587 2778 33
Size: 316 279 27 254 685 993 191 1121
Fragments arranged by size:
1121 993 685 316 279 254 191 27
SfoI GGC'GCC
Cuts at: 1298 1546 1298
Size: 248 3618
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
SmaI CCC'GGG
Cuts at: 888 888
Size: 3866
SmlI C'TyrA_G
Cuts at: 756 1021 1936 2428 2690 756
Size: 265 915 492 262 1932
Fragments arranged by size:
1932 915 492 265 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3866
SphI G_CATG'C
Cuts at: 919 1419 919
Size: 500 3366
StyI C'CwwG_G
Cuts at: 107 392 497 1000 1354 107
Size: 285 105 503 354 2619
Fragments arranged by size:
2619 503 354 285 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 314 790 1449 1582 2041 2250 314
Size: 476 659 133 459 209 1930
Fragments arranged by size:
1930 659 476 459 209 133
TatI w'GTAC_w
Cuts at: 221 359 3381 221
Size: 138 3022 706
Fragments arranged by size:
3022 706 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 734 766 3383 3415 734
Size: 32 2617 32 1185
Fragments arranged by size:
2617 1185 32 32
Tth111I GACn'n_nGTC
Cuts at: 1663 1663
Size: 3866
XbaI T'CTAG_A
Cuts at: 897 897
Size: 3866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1361 1361
Size: 3866
XmaI C'CCGG_G
Cuts at: 886 886
Size: 3866
ZraI GAC'GTC
Cuts at: 651 651
Size: 3866
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PmlI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsgI BsiWI BsmI BsmBI
BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PpuMI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI