Restriction map for
pF1KB9782
(Circular) (Six-base) MAPSORT of: pF1KB9782.seq Check: 266 from: 1 to: 3740
pF1KB9782 3740 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 778 778
Size: 3740
Acc65I G'GTAC_C
Cuts at: 756 756
Size: 3740
AclI AA'CG_TT
Cuts at: 3580 3580
Size: 3740
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 505 1565 1997 2744 3012 412
Size: 93 1060 432 747 268 1140
Fragments arranged by size:
1140 1060 747 432 268 93
AflIII A'CryG_T
Cuts at: 1092 2196 3173 1092
Size: 1104 977 1659
Fragments arranged by size:
1659 1104 977
AgeI A'CCGG_T
Cuts at: 2128 2128
Size: 3740
AlwNI CAG_nnn'CTG
Cuts at: 1045 2612 1045
Size: 1567 2173
ApaI G_GGCC'C
Cuts at: 358 701 358
Size: 343 3397
ApaLI G'TGCA_C
Cuts at: 406 2510 406
Size: 2104 1636
ApoI r'AATT_y
Cuts at: 744 3093 3349 744
Size: 2349 256 1135
Fragments arranged by size:
2349 1135 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3740
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
AvaI C'yCGr_G
Cuts at: 760 760
Size: 3740
BamHI G'GATC_C
Cuts at: 765 765
Size: 3740
BanI G'GyrC_C
Cuts at: 345 491 673 756 1170 1418 1453 345
Size: 146 182 83 414 248 35 2632
Fragments arranged by size:
2632 414 248 182 146 83 35
BanII G_rGCy'C
Cuts at: 358 550 701 754 1784 358
Size: 192 151 53 1030 2314
Fragments arranged by size:
2314 1030 192 151 53
BbeI G_GCGC'C
Cuts at: 495 1174 1422 495
Size: 679 248 2813
Fragments arranged by size:
2813 679 248
BbsI GAAGACnn'nnnn_
Cuts at: 266 266
Size: 3740
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 457 491 457
Size: 34 3706
BciVI GTATCCnnnnn_n'
Cuts at: 1638 2399 3740 1638
Size: 761 1341 1638
Fragments arranged by size:
1638 1341 761
BclI T'GATC_A
Cuts at: 1260 1260
Size: 3740
BglII A'GATC_T
Cuts at: 1255 3292 1255
Size: 2037 1703
BlpI GC'TnA_GC
Cuts at: 382 852 382
Size: 470 3270
Bme1580I G_kGCm'C
Cuts at: 350 358 410 444 701 1365 1458 2514 350
Size: 8 52 34 257 664 93 1056 1576
Fragments arranged by size:
1576 1056 664 257 93 52 34 8
BmrI ACTGGGnnnn_n'
Cuts at: 952 1033 1368 3512 952
Size: 81 335 2144 1180
Fragments arranged by size:
2144 1180 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 135 328 2107 135
Size: 193 1779 1768
Fragments arranged by size:
1779 1768 193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 175 442 517 157
Size: 18 267 75 3380
Fragments arranged by size:
3380 267 75 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 346 916 1795 2287 2585 319
Size: 27 570 879 492 298 1474
Fragments arranged by size:
1474 879 570 492 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 3444 3444
Size: 3740
BsaAI yAC'GTr
Cuts at: 1093 1093
Size: 3740
BsaBI GATnn'nnATC
Cuts at: 6 1254 3291 6
Size: 1248 2037 455
Fragments arranged by size:
2037 1248 455
BsaHI Gr'CG_yC
Cuts at: 492 1171 1419 2121 3617 492
Size: 679 248 702 1496 615
Fragments arranged by size:
1496 702 679 615 248
BsaWI w'CCGG_w
Cuts at: 929 1450 2128 2402 2549 2960 929
Size: 521 678 274 147 411 1709
Fragments arranged by size:
1709 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 490 520 490
Size: 30 3710
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 439 439
Size: 3740
BseYI C'CCAG_C
Cuts at: 714 1165 1923 2500 2996 714
Size: 451 758 577 496 1458
Fragments arranged by size:
1458 758 577 496 451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 532
Size: 3740
BsiEI CG_ry'CG
Cuts at: 104 146 242 1282 1328 2536 104
Size: 42 96 1040 46 1208 1308
Fragments arranged by size:
1308 1208 1040 96 46 42
BsiHKAI G_wGCw'C
Cuts at: 182 410 754 1532 1722 2514 182
Size: 228 344 778 190 792 1408
Fragments arranged by size:
1408 792 778 344 228 190
Bsp1286I G_dGCh'C
Cuts at: 182 350 358 410 444 550 701 754 1365
Size: 168 8 52 34 106 151 53 611
Cuts at: 1365 1458 1532 1722 1784 2514 182
Size: 93 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 190 168 151 106 93
74 62 53 52 34 8
BspEI T'CCGG_A
Cuts at: 2960 2960
Size: 3740
BspHI T'CATG_A
Cuts at: 2916 2916
Size: 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 790 1004 1306 1687 790
Size: 214 302 381 2843
Fragments arranged by size:
2843 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2032 2086 3570 3735 50
Size: 1982 54 1484 165 55
Fragments arranged by size:
1982 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1652 1652
Size: 3740
BsrFI r'CCGG_y
Cuts at: 241 676 707 1738 1919 2128 241
Size: 435 31 1031 181 209 1853
Fragments arranged by size:
1853 1031 435 209 181 31
BssSI C'ACGA_G
Cuts at: 2011 2369 2011
Size: 358 3382
BstAPI GCAn_nnn'nTGC
Cuts at: 345 1117 345
Size: 772 2968
BstBI TT'CG_AA
Cuts at: 2101 2101
Size: 3740
BstYI r'GATC_y
Cuts at: 1 765 1232 1255 1590 1836 2848 2934 2946
Size: 764 467 23 335 246 1012 86 12
Cuts at: 2946 3292 1
Size: 346 449
Fragments arranged by size:
1012 764 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 560 107
Size: 453 3287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1690 1871 2034 1690
Size: 181 163 3396
Fragments arranged by size:
3396 181 163
BtsI GCAGTG_nn'
Cuts at: 645 702 983 645
Size: 57 281 3402
Fragments arranged by size:
3402 281 57
DraI TTT'AAA
Cuts at: 739 739
Size: 3740
DrdI GACnn_nn'nnGTC
Cuts at: 1446 2304 1446
Size: 858 2882
EaeI y'GGCC_r
Cuts at: 143 239 1325 1499 1890 1917 3262 143
Size: 96 1086 174 391 27 1345 621
Fragments arranged by size:
1345 1086 621 391 174 96 27
EagI C'GGCC_G
Cuts at: 143 239 1325 143
Size: 96 1086 2558
Fragments arranged by size:
2558 1086 96
EarI CTCTTCn'nnn_
Cuts at: 1763 1973 1763
Size: 210 3530
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1948 2258 2404 3550 1948
Size: 310 146 1146 2138
Fragments arranged by size:
2138 1146 310 146
EcoICRI GAG'CTC
Cuts at: 752 752
Size: 3740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 175 412 442 505 517 1565 1997 2744
Size: 18 237 30 63 12 1048 432 747
Cuts at: 2744 3012 157
Size: 268 885
Fragments arranged by size:
1048 885 747 432 268 237 63 30
18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 136 136
Size: 3740
EcoO109I rG'GnC_Cy
Cuts at: 258 355 698 879 258
Size: 97 343 181 3119
Fragments arranged by size:
3119 343 181 97
EcoRI G'AATT_C
Cuts at: 744 744
Size: 3740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1759 1759
Size: 3740
FseI GG_CCGG'CC
Cuts at: 245 245
Size: 3740
FspI TGC'GCA
Cuts at: 253 532 1521 253
Size: 279 989 2472
Fragments arranged by size:
2472 989 279
FspAI rTGC'GCAy
Cuts at: 532 532
Size: 3740
HaeII r_GCGC'y
Cuts at: 495 1174 1422 2444 3423 495
Size: 679 248 1022 979 812
Fragments arranged by size:
1022 979 812 679 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 490 522 1582 1612 1614 1644 490
Size: 32 1060 30 2 30 2586
Fragments arranged by size:
2586 1060 32 30 30 2
HincII GTy'rAC
Cuts at: 779 779
Size: 3740
KasI G'GCGC_C
Cuts at: 491 1170 1418 491
Size: 679 248 2813
Fragments arranged by size:
2813 679 248
KpnI G_GTAC'C
Cuts at: 760 760
Size: 3740
MluI A'CGCG_T
Cuts at: 3173 3173
Size: 3740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2411 2595 2411
Size: 184 3556
MscI TGG'CCA
Cuts at: 1501 1501
Size: 3740
MslI CAynn'nnrTG
Cuts at: 1729 1856 1729
Size: 127 3613
MspA1I CmG'CkG
Cuts at: 462 504 851 1165 1525 2538 2783 462
Size: 42 347 314 360 1013 245 1419
Fragments arranged by size:
1419 1013 360 347 314 245 42
NaeI GCC'GGC
Cuts at: 243 709 1921 243
Size: 466 1212 2062
Fragments arranged by size:
2062 1212 466
NarI GG'CG_CC
Cuts at: 492 1171 1419 492
Size: 679 248 2813
Fragments arranged by size:
2813 679 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3740
NgoMIV G'CCGG_C
Cuts at: 241 707 1919 241
Size: 466 1212 2062
Fragments arranged by size:
2062 1212 466
NspI r_CATG'y
Cuts at: 793 1293 2200 793
Size: 500 907 2333
Fragments arranged by size:
2333 907 500
PasI CC'CwG_GG
Cuts at: 351 351
Size: 3740
PciI A'CATG_T
Cuts at: 2196 2196
Size: 3740
PmeI GTTT'AAAC
Cuts at: 739 739
Size: 3740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2879 2911 2879
Size: 32 3708
PspOMI G'GGCC_C
Cuts at: 354 697 354
Size: 343 3397
PstI C_TGCA'G
Cuts at: 467 668 787 1472 467
Size: 201 119 685 2735
Fragments arranged by size:
2735 685 201 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3740
PvuII CAG'CTG
Cuts at: 462 504 1165 1525 462
Size: 42 661 360 2677
Fragments arranged by size:
2677 661 360 42
RsrII CG'GwC_CG
Cuts at: 1935 1935
Size: 3740
SacI G_AGCT'C
Cuts at: 754 754
Size: 3740
SalI G'TCGA_C
Cuts at: 777 777
Size: 3740
SapI GCTCTTCn'nnn_
Cuts at: 1763 1973 1763
Size: 210 3530
SbfI CC_TGCA'GG
Cuts at: 787 787
Size: 3740
ScaI AGT'ACT
Cuts at: 3257 3257
Size: 3740
SfcI C'TryA_G
Cuts at: 33 463 657 664 783 1468 2461 2652 33
Size: 430 194 7 119 685 993 191 1121
Fragments arranged by size:
1121 993 685 430 194 191 119 7
SfoI GGC'GCC
Cuts at: 493 1172 1420 493
Size: 679 248 2813
Fragments arranged by size:
2813 679 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
SgrAI Cr'CCGG_yG
Cuts at: 676 676
Size: 3740
SmaI CCC'GGG
Cuts at: 762 762
Size: 3740
SmlI C'TyrA_G
Cuts at: 325 334 895 1810 2302 2564 325
Size: 9 561 915 492 262 1501
Fragments arranged by size:
1501 915 561 492 262 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3740
SphI G_CATG'C
Cuts at: 793 1293 793
Size: 500 3240
StyI C'CwwG_G
Cuts at: 107 401 874 1228 107
Size: 294 473 354 2619
Fragments arranged by size:
2619 473 354 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1323 1456 1915 2124 1323
Size: 133 459 209 2939
Fragments arranged by size:
2939 459 209 133
TatI w'GTAC_w
Cuts at: 3255 3255
Size: 3740
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 3708
Tth111I GACn'n_nGTC
Cuts at: 1537 1537
Size: 3740
XbaI T'CTAG_A
Cuts at: 771 771
Size: 3740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1235 1235
Size: 3740
XmaI C'CCGG_G
Cuts at: 760 760
Size: 3740
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI