Restriction map for
pF1KB9775
(Circular) (Six-base) MAPSORT of: pF1KB9775.seq Check: 7393 from: 1 to: 5147
pF1KB9775 5147 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2096 2096
Size: 5147
AatII G_ACGT'C
Cuts at: 404 404
Size: 5147
AccI GT'mk_AC
Cuts at: 383 2185 383
Size: 1802 3345
Acc65I G'GTAC_C
Cuts at: 2163 2163
Size: 5147
AclI AA'CG_TT
Cuts at: 4987 4987
Size: 5147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 383 551 619 1243 1845 2972 3404 3757
Size: 52 168 68 624 602 1127 432 353
Cuts at: 3757 4419 331
Size: 662 1059
Fragments arranged by size:
1127 1059 662 624 602 432 353 168
68 52
AfeI AGC'GCT
Cuts at: 148 1639 148
Size: 1491 3656
AflII C'TTAA_G
Cuts at: 897 897
Size: 5147
AflIII A'CryG_T
Cuts at: 2499 4299 4580 2499
Size: 1800 281 3066
Fragments arranged by size:
3066 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 165 462 597 1131 1414 1452 1704 2452 3890
Size: 297 135 534 283 38 252 748 1438
Cuts at: 3890 165
Size: 1422
Fragments arranged by size:
1438 1422 748 534 297 283 252 135
38
ApaLI G'TGCA_C
Cuts at: 3985 3985
Size: 5147
ApoI r'AATT_y
Cuts at: 523 1095 2151 4500 4756 523
Size: 572 1056 2349 256 914
Fragments arranged by size:
2349 1056 914 572 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5147
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5147
AvaI C'yCGr_G
Cuts at: 2167 2167
Size: 5147
BamHI G'GATC_C
Cuts at: 2172 2172
Size: 5147
BanI G'GyrC_C
Cuts at: 957 1492 1579 2163 2577 2825 2860 957
Size: 535 87 584 414 248 35 3244
Fragments arranged by size:
3244 584 535 414 248 87 35
BanII G_rGCy'C
Cuts at: 1529 2161 3191 1529
Size: 632 1030 3485
Fragments arranged by size:
3485 1030 632
BbeI G_GCGC'C
Cuts at: 2581 2829 2581
Size: 248 4899
BbsI GAAGACnn'nnnn_
Cuts at: 1995 1995
Size: 5147
BbvCI CC'TCA_GC
Cuts at: 1921 1921
Size: 5147
BciVI GTATCCnnnnn_n'
Cuts at: 3045 4101 5147 3045
Size: 1056 1046 3045
Fragments arranged by size:
3045 1056 1046
BclI T'GATC_A
Cuts at: 833 2667 833
Size: 1834 3313
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5147
BglII A'GATC_T
Cuts at: 1109 2662 4699 1109
Size: 1553 2037 1557
Fragments arranged by size:
2037 1557 1553
BlpI GC'TnA_GC
Cuts at: 719 1132 1499 2259 719
Size: 413 367 760 3607
Fragments arranged by size:
3607 760 413 367
Bme1580I G_kGCm'C
Cuts at: 1584 1650 2772 2865 3989 1584
Size: 66 1122 93 1124 2742
Fragments arranged by size:
2742 1124 1122 93 66
BmgBI CAC'GTC
Cuts at: 966 1056 966
Size: 90 5057
BmrI ACTGGGnnnn_n'
Cuts at: 1821 2359 2440 2775 4919 1821
Size: 538 81 335 2144 2049
Fragments arranged by size:
2144 2049 538 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3514 3514
Size: 5147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 427 730 916 981 1051 1303 1318 1462
Size: 167 303 186 65 70 252 15 144
Cuts at: 1462 1531 1608 1681 260
Size: 69 77 73 3726
Fragments arranged by size:
3726 303 252 186 167 144 77 73
70 69 65 15
Bpu10I CC'TnA_GC
Cuts at: 1357 1453 1891 1921 1357
Size: 96 438 30 4583
Fragments arranged by size:
4583 438 96 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1141 1684 1917 2323 3202 3916 4214 646
Size: 495 543 233 406 879 714 298 1579
Fragments arranged by size:
1579 879 714 543 495 406 298 233
BsaI GGTCTCn'nnnn_
Cuts at: 886 4851 886
Size: 3965 1182
BsaAI yAC'GTr
Cuts at: 2500 2500
Size: 5147
BsaBI GATnn'nnATC
Cuts at: 6 2661 4698 6
Size: 2655 2037 455
Fragments arranged by size:
2655 2037 455
BsaHI Gr'CG_yC
Cuts at: 401 1932 2578 2826 3528 5024 401
Size: 1531 646 248 702 1496 524
Fragments arranged by size:
1531 1496 702 646 524 248
BsaWI w'CCGG_w
Cuts at: 2336 2857 3535 3946 4093 4367 2336
Size: 521 678 411 147 274 3116
Fragments arranged by size:
3116 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 425 455 1276 1306 1801 1831 1837 1867 425
Size: 30 821 30 495 30 6 30 3705
Fragments arranged by size:
3705 821 495 30 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1845 1849 1845
Size: 4 5143
BseYI C'CCAG_C
Cuts at: 1255 1685 2572 3330 3995 4403 1255
Size: 430 887 758 665 408 1999
Fragments arranged by size:
1999 887 758 665 430 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 475 1078 1391 1975 2074 448
Size: 27 603 313 584 99 3521
Fragments arranged by size:
3521 603 584 313 99 27
BsiEI CG_ry'CG
Cuts at: 104 2689 2735 3965 104
Size: 2585 46 1230 1286
Fragments arranged by size:
2585 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1505 2015 2161 2939 3129 3989 1505
Size: 510 146 778 190 860 2663
Fragments arranged by size:
2663 860 778 510 190 146
Bsp1286I G_dGCh'C
Cuts at: 1505 1529 1584 1650 2015 2161 2772 2865 2939
Size: 24 55 66 365 146 611 93 74
Cuts at: 2939 3129 3191 3989 1505
Size: 190 62 798 2663
Fragments arranged by size:
2663 798 611 365 190 146 93 74
66 62 55 24
BspHI T'CATG_A
Cuts at: 836 3579 836
Size: 2743 2404
BspMI ACCTGCnnnn'nnnn_
Cuts at: 285 1569 2096 2130 2197 2411 2713 3094 285
Size: 1284 527 34 67 214 302 381 2338
Fragments arranged by size:
2338 1284 527 381 302 214 67 34
BsrBI CCG'CTC
Cuts at: 50 995 1029 1310 1361 1875 3439 3493 4977
Size: 945 34 281 51 514 1564 54 1484
Cuts at: 4977 5142 50
Size: 165 55
Fragments arranged by size:
1564 1484 945 514 281 165 55 54
51 34
BsrDI GCAATG_nn'
Cuts at: 3059 3059
Size: 5147
BsrFI r'CCGG_y
Cuts at: 206 3145 3326 206
Size: 2939 181 2027
Fragments arranged by size:
2939 2027 181
BssHII G'CGCG_C
Cuts at: 1728 1957 1728
Size: 229 4918
BssSI C'ACGA_G
Cuts at: 2101 3418 4126 2101
Size: 1317 708 3122
Fragments arranged by size:
3122 1317 708
BstAPI GCAn_nnn'nTGC
Cuts at: 462 2524 462
Size: 2062 3085
BstBI TT'CG_AA
Cuts at: 3508 3508
Size: 5147
BstXI CCAn_nnnn'nTGG
Cuts at: 1281 1953 1281
Size: 672 4475
BstYI r'GATC_y
Cuts at: 1 408 426 947 1109 1517 2172 2639 2662
Size: 407 18 521 162 408 655 467 23
Cuts at: 2662 2997 3243 3549 3561 3647 4699 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 655 521 467 449 408 407 335
306 246 162 86 23 18 12
BtgI C'CryG_G
Cuts at: 107 1229 107
Size: 1122 4025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3097 3278 3441 3097
Size: 181 163 4803
Fragments arranged by size:
4803 181 163
BtsI GCAGTG_nn'
Cuts at: 1541 1606 1803 1992 2390 1541
Size: 65 197 189 398 4298
Fragments arranged by size:
4298 398 197 189 65
ClaI AT'CG_AT
Cuts at: 127 127
Size: 5147
DraI TTT'AAA
Cuts at: 2146 2146
Size: 5147
DrdI GACnn_nn'nnGTC
Cuts at: 690 2853 4197 690
Size: 2163 1344 1640
Fragments arranged by size:
2163 1640 1344
EaeI y'GGCC_r
Cuts at: 113 917 1016 1100 1271 1403 1782 2732 2906
Size: 804 99 84 171 132 379 950 174
Cuts at: 2906 3297 3324 4669 113
Size: 391 27 1345 591
Fragments arranged by size:
1345 950 804 591 391 379 174 171
132 99 84 27
EagI C'GGCC_G
Cuts at: 2732 2732
Size: 5147
EarI CTCTTCn'nnn_
Cuts at: 307 1190 3170 3380 307
Size: 883 1980 210 2074
Fragments arranged by size:
2074 1980 883 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 904 3355 4097 4243 4957 904
Size: 2451 742 146 714 1094
Fragments arranged by size:
2451 1094 742 714 146
EcoICRI GAG'CTC
Cuts at: 2159 2159
Size: 5147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 331 383 427 551 619 730 916 981
Size: 71 52 44 124 68 111 186 65
Cuts at: 981 1051 1243 1303 1318 1462 1531 1608 1681
Size: 70 192 60 15 144 69 77 73
Cuts at: 1681 1845 2972 3404 3757 4419 260
Size: 164 1127 432 353 662 988
Fragments arranged by size:
1127 988 662 432 353 192 186 164
144 124 111 77 73 71 70 69
68 65 60 52 44 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1444 1588 1444
Size: 144 5003
EcoO109I rG'GnC_Cy
Cuts at: 1449 1701 2286 1449
Size: 252 585 4310
Fragments arranged by size:
4310 585 252
EcoRI G'AATT_C
Cuts at: 1095 2151 1095
Size: 1056 4091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3166 3166
Size: 5147
FspI TGC'GCA
Cuts at: 289 1339 2928 289
Size: 1050 1589 2508
Fragments arranged by size:
2508 1589 1050
HaeII r_GCGC'y
Cuts at: 150 304 462 1139 1256 1641 1842 2581 2829
Size: 154 158 677 117 385 201 739 248
Cuts at: 2829 4059 4830 150
Size: 1230 771 467
Fragments arranged by size:
1230 771 739 677 467 385 248 201
158 154 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 425 457 1415 1447 1837 1869 1884 1916 2989
Size: 32 958 32 390 32 15 32 1073
Cuts at: 2989 3019 3021 3051 425
Size: 30 2 30 2521
Fragments arranged by size:
2521 1073 958 390 32 32 32 32
30 30 15 2
HincII GTy'rAC
Cuts at: 2186 2186
Size: 5147
KasI G'GCGC_C
Cuts at: 2577 2825 2577
Size: 248 4899
KpnI G_GTAC'C
Cuts at: 2167 2167
Size: 5147
MluI A'CGCG_T
Cuts at: 4580 4580
Size: 5147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1370 1970 3906 4090 1370
Size: 600 1936 184 2427
Fragments arranged by size:
2427 1936 600 184
MscI TGG'CCA
Cuts at: 1405 2908 1405
Size: 1503 3644
MslI CAynn'nnrTG
Cuts at: 982 2105 3136 3263 982
Size: 1123 1031 127 2866
Fragments arranged by size:
2866 1123 1031 127
MspA1I CmG'CkG
Cuts at: 165 306 936 1044 1131 1476 1498 1577 1793
Size: 141 630 108 87 345 22 79 216
Cuts at: 1793 2258 2572 2932 3716 3961 165
Size: 465 314 360 784 245 1351
Fragments arranged by size:
1351 784 630 465 360 345 314 245
216 141 108 87 79 22
NaeI GCC'GGC
Cuts at: 3328 3328
Size: 5147
NarI GG'CG_CC
Cuts at: 2578 2826 2578
Size: 248 4899
NcoI C'CATG_G
Cuts at: 107 1229 107
Size: 1122 4025
NgoMIV G'CCGG_C
Cuts at: 3326 3326
Size: 5147
NspI r_CATG'y
Cuts at: 2200 2700 4303 2200
Size: 500 1603 3044
Fragments arranged by size:
3044 1603 500
PasI CC'CwG_GG
Cuts at: 1904 1904
Size: 5147
PciI A'CATG_T
Cuts at: 4299 4299
Size: 5147
PmeI GTTT'AAAC
Cuts at: 2146 2146
Size: 5147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 5115
PpuMI rG'GwC_Cy
Cuts at: 1449 1449
Size: 5147
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 626 658 626
Size: 32 5115
PstI C_TGCA'G
Cuts at: 170 680 1319 1566 1709 1772 2194 2879 170
Size: 510 639 247 143 63 422 685 2438
Fragments arranged by size:
2438 685 639 510 422 247 143 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5147
PvuII CAG'CTG
Cuts at: 165 936 1044 1131 2572 2932 165
Size: 771 108 87 1441 360 2380
Fragments arranged by size:
2380 1441 771 360 108 87
RsrII CG'GwC_CG
Cuts at: 3342 3342
Size: 5147
SacI G_AGCT'C
Cuts at: 2161 2161
Size: 5147
SalI G'TCGA_C
Cuts at: 2184 2184
Size: 5147
SapI GCTCTTCn'nnn_
Cuts at: 3170 3380 3170
Size: 210 4937
SbfI CC_TGCA'GG
Cuts at: 2194 2194
Size: 5147
ScaI AGT'ACT
Cuts at: 4664 4664
Size: 5147
SfcI C'TryA_G
Cuts at: 33 166 676 1315 1562 1705 1768 2190 2875
Size: 133 510 639 247 143 63 422 685
Cuts at: 2875 3843 4034 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 639 510 422 247 191
143 133 63
SfoI GGC'GCC
Cuts at: 2579 2827 2579
Size: 248 4899
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5147
SmaI CCC'GGG
Cuts at: 2169 2169
Size: 5147
SmlI C'TyrA_G
Cuts at: 625 897 1120 1663 1896 2302 3217 3931 4193
Size: 272 223 543 233 406 915 714 262
Cuts at: 4193 625
Size: 1579
Fragments arranged by size:
1579 915 714 543 406 272 262 233
223
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5147
SphI G_CATG'C
Cuts at: 2200 2700 2200
Size: 500 4647
SspI AAT'ATT
Cuts at: 139 139
Size: 5147
StyI C'CwwG_G
Cuts at: 107 321 1022 1229 2281 2635 107
Size: 214 701 207 1052 354 2619
Fragments arranged by size:
2619 1052 701 354 214 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2730 2863 3322 3531 2730
Size: 133 459 209 4346
Fragments arranged by size:
4346 459 209 133
TatI w'GTAC_w
Cuts at: 4662 4662
Size: 5147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4664 4696 4664
Size: 32 5115
Tth111I GACn'n_nGTC
Cuts at: 2944 2944
Size: 5147
XbaI T'CTAG_A
Cuts at: 2178 2178
Size: 5147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 705 1923 2642 705
Size: 1218 719 3210
Fragments arranged by size:
3210 1218 719
XmaI C'CCGG_G
Cuts at: 2167 2167
Size: 5147
XmnI GAAnn'nnTTC
Cuts at: 316 316
Size: 5147
ZraI GAC'GTC
Cuts at: 402 402
Size: 5147
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AgeI AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BspEI
BsrGI BstEII BstZ17I Bsu36I CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI