Restriction map for
pF1KB9765
(Circular) (Six-base) MAPSORT of: pF1KB9765.seq Check: 1609 from: 1 to: 5822
pF1KB9765 5822 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 2486 2768 2860 2486
Size: 282 92 5448
Fragments arranged by size:
5448 282 92
Acc65I G'GTAC_C
Cuts at: 2838 2838
Size: 5822
AclI AA'CG_TT
Cuts at: 156 5662 156
Size: 5506 316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1154 3647 4079 4826 5094 1154
Size: 2493 432 747 268 1882
Fragments arranged by size:
2493 1882 747 432 268
AfeI AGC'GCT
Cuts at: 1693 1693
Size: 5822
AflIII A'CryG_T
Cuts at: 1532 2657 2720 3174 4278 5255 1532
Size: 1125 63 454 1104 977 2099
Fragments arranged by size:
2099 1125 1104 977 454 63
AgeI A'CCGG_T
Cuts at: 4210 4210
Size: 5822
AhdI GACnn_n'nnGTC
Cuts at: 1410 2256 1410
Size: 846 4976
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2437 2566 2437
Size: 129 5693
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1447 1479 1447
Size: 32 5790
AlwNI CAG_nnn'CTG
Cuts at: 489 2009 2799 3127 4694 489
Size: 1520 790 328 1567 1617
Fragments arranged by size:
1617 1567 1520 790 328
ApaLI G'TGCA_C
Cuts at: 410 434 844 2434 2644 4592 410
Size: 24 410 1590 210 1948 1640
Fragments arranged by size:
1948 1640 1590 410 210 24
ApoI r'AATT_y
Cuts at: 526 2826 5175 5431 526
Size: 2300 2349 256 917
Fragments arranged by size:
2349 2300 917 256
AseI AT'TA_AT
Cuts at: 20 1265 20
Size: 1245 4577
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5822
AvaI C'yCGr_G
Cuts at: 185 742 1022 2296 2842 185
Size: 557 280 1274 546 3165
Fragments arranged by size:
3165 1274 557 546 280
AvrII C'CTAG_G
Cuts at: 561 561
Size: 5822
BamHI G'GATC_C
Cuts at: 138 2847 138
Size: 2709 3113
BanI G'GyrC_C
Cuts at: 111 1862 2176 2212 2497 2838 3252 3500 3535
Size: 1751 314 36 285 341 414 248 35
Cuts at: 3535 111
Size: 2398
Fragments arranged by size:
2398 1751 414 341 314 285 248 36
35
BanII G_rGCy'C
Cuts at: 1511 1885 1916 2836 3866 1511
Size: 374 31 920 1030 3467
Fragments arranged by size:
3467 1030 920 374 31
BbeI G_GCGC'C
Cuts at: 115 1866 2180 2216 3256 3504 115
Size: 1751 314 36 1040 248 2433
Fragments arranged by size:
2433 1751 1040 314 248 36
BbsI GAAGACnn'nnnn_
Cuts at: 1142 1505 1142
Size: 363 5459
BbvCI CC'TCA_GC
Cuts at: 695 695
Size: 5822
BciVI GTATCCnnnnn_n'
Cuts at: 1232 3720 4481 5822 1232
Size: 2488 761 1341 1232
Fragments arranged by size:
2488 1341 1232 761
BclI T'GATC_A
Cuts at: 3342 3342
Size: 5822
BglI GCCn_nnn'nGGC
Cuts at: 1889 1920 2227 1889
Size: 31 307 5484
Fragments arranged by size:
5484 307 31
BglII A'GATC_T
Cuts at: 1970 2033 3337 5374 1970
Size: 63 1304 2037 2418
Fragments arranged by size:
2418 2037 1304 63
BlpI GC'TnA_GC
Cuts at: 638 711 2934 638
Size: 73 2223 3526
Fragments arranged by size:
3526 2223 73
Bme1580I G_kGCm'C
Cuts at: 414 438 691 848 2438 2500 2648 3447 3540
Size: 24 253 157 1590 62 148 799 93
Cuts at: 3540 4596 414
Size: 1056 1640
Fragments arranged by size:
1640 1590 1056 799 253 157 148 93
62 24
BmgBI CAC'GTC
Cuts at: 1140 1745 1140
Size: 605 5217
BmrI ACTGGGnnnn_n'
Cuts at: 425 761 2387 3034 3115 3450 5594 425
Size: 336 1626 647 81 335 2144 653
Fragments arranged by size:
2144 1626 653 647 336 335 81
BmtI G_CTAG'C
Cuts at: 2696 2696
Size: 5822
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1434 4189 1434
Size: 2755 3067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1560 1782 2022 2407 2821 1560
Size: 222 240 385 414 4561
Fragments arranged by size:
4561 414 385 240 222
Bpu10I CC'TnA_GC
Cuts at: 695 695
Size: 5822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1939 2998 3877 4369 4667 814
Size: 1125 1059 879 492 298 1969
Fragments arranged by size:
1969 1125 1059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 679 730 952 5526 679
Size: 51 222 4574 975
Fragments arranged by size:
4574 975 222 51
BsaAI yAC'GTr
Cuts at: 627 1698 2142 2643 3175 627
Size: 1071 444 501 532 3274
Fragments arranged by size:
3274 1071 532 501 444
BsaBI GATnn'nnATC
Cuts at: 6 1678 3336 5373 6
Size: 1672 1658 2037 455
Fragments arranged by size:
2037 1672 1658 455
BsaHI Gr'CG_yC
Cuts at: 112 1863 2177 2213 2275 2617 3253 3501 4203
Size: 1751 314 36 62 342 636 248 702
Cuts at: 4203 5699 112
Size: 1496 235
Fragments arranged by size:
1751 1496 702 636 342 314 248 235
62 36
BsaWI w'CCGG_w
Cuts at: 1514 3011 3532 4210 4484 4631 5042 1514
Size: 1497 521 678 274 147 411 2294
Fragments arranged by size:
2294 1497 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 611 641 1441 1471 1693 1723 611
Size: 30 800 30 222 30 4710
Fragments arranged by size:
4710 800 222 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 714 714
Size: 5822
BseYI C'CCAG_C
Cuts at: 124 645 931 2074 2350 3247 4005 4582 5078
Size: 521 286 1143 276 897 758 577 496
Cuts at: 5078 124
Size: 868
Fragments arranged by size:
1143 897 868 758 577 521 496 286
276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2122 2122
Size: 5822
BsiEI CG_ry'CG
Cuts at: 104 1515 2192 2474 2735 3364 3410 4618 104
Size: 1411 677 282 261 629 46 1208 1308
Fragments arranged by size:
1411 1308 1208 677 629 282 261 46
BsiHKAI G_wGCw'C
Cuts at: 414 438 848 1619 1665 1955 2087 2426 2438
Size: 24 410 771 46 290 132 339 12
Cuts at: 2438 2648 2836 3614 3804 4596 414
Size: 210 188 778 190 792 1640
Fragments arranged by size:
1640 792 778 771 410 339 290 210
190 188 132 46 24 12
BsmI GAATG_Cn'
Cuts at: 1281 1281
Size: 5822
Bsp1286I G_dGCh'C
Cuts at: 414 438 691 848 1511 1619 1665 1885 1916
Size: 24 253 157 663 108 46 220 31
Cuts at: 1916 1955 2087 2426 2438 2500 2648 2836 3447
Size: 39 132 339 12 62 148 188 611
Cuts at: 3447 3540 3614 3804 3866 4596 414
Size: 93 74 190 62 730 1640
Fragments arranged by size:
1640 730 663 611 339 253 220 190
188 157 148 132 108 93 74 62
62 46 39 31 24 12
BspEI T'CCGG_A
Cuts at: 5042 5042
Size: 5822
BspHI T'CATG_A
Cuts at: 4998 4998
Size: 5822
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1724 2555 2872 3086 3388 3769 1724
Size: 831 317 214 302 381 3777
Fragments arranged by size:
3777 831 381 317 302 214
BsrBI CCG'CTC
Cuts at: 50 2268 4114 4168 5652 5817 50
Size: 2218 1846 54 1484 165 55
Fragments arranged by size:
2218 1846 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3734 3734
Size: 5822
BsrFI r'CCGG_y
Cuts at: 887 1859 2648 2713 3820 4001 4210 887
Size: 972 789 65 1107 181 209 2499
Fragments arranged by size:
2499 1107 972 789 209 181 65
BsrGI T'GTAC_A
Cuts at: 533 2372 533
Size: 1839 3983
BssHII G'CGCG_C
Cuts at: 1787 1787
Size: 5822
BssSI C'ACGA_G
Cuts at: 949 4093 4451 949
Size: 3144 358 2320
Fragments arranged by size:
3144 2320 358
BstAPI GCAn_nnn'nTGC
Cuts at: 969 2403 3199 969
Size: 1434 796 3592
Fragments arranged by size:
3592 1434 796
BstBI TT'CG_AA
Cuts at: 4183 4183
Size: 5822
BstEII G'GTnAC_C
Cuts at: 2442 2523 2442
Size: 81 5741
BstYI r'GATC_y
Cuts at: 1 138 1080 1970 2033 2847 3314 3337 3672
Size: 137 942 890 63 814 467 23 335
Cuts at: 3672 3918 4930 5016 5028 5374 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 942 890 814 467 449 346 335
246 137 86 63 23 12
Bsu36I CC'TnA_GG
Cuts at: 1431 1431
Size: 5822
BtgI C'CryG_G
Cuts at: 107 355 915 1343 1918 2225 2281 2492 2621
Size: 248 560 428 575 307 56 211 129
Cuts at: 2621 2729 107
Size: 108 3200
Fragments arranged by size:
3200 575 560 428 307 248 211 129
108 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1335 1687 3772 3953 4116 1335
Size: 352 2085 181 163 3041
Fragments arranged by size:
3041 2085 352 181 163
BtsI GCAGTG_nn'
Cuts at: 600 689 2687 3065 600
Size: 89 1998 378 3357
Fragments arranged by size:
3357 1998 378 89
DraI TTT'AAA
Cuts at: 2821 2821
Size: 5822
DraIII CAC_nnn'GTG
Cuts at: 2442 2643 2442
Size: 201 5621
DrdI GACnn_nn'nnGTC
Cuts at: 3528 4386 3528
Size: 858 4964
EaeI y'GGCC_r
Cuts at: 239 1566 2144 2189 2219 2456 2471 2531 2732
Size: 1327 578 45 30 237 15 60 201
Cuts at: 2732 3407 3581 3972 3999 5344 239
Size: 675 174 391 27 1345 717
Fragments arranged by size:
1345 1327 717 675 578 391 237 201
174 60 45 30 27 15
EagI C'GGCC_G
Cuts at: 2189 2471 2732 3407 2189
Size: 282 261 675 4604
Fragments arranged by size:
4604 675 282 261
EarI CTCTTCn'nnn_
Cuts at: 260 1102 2749 3845 4055 260
Size: 842 1647 1096 210 2027
Fragments arranged by size:
2027 1647 1096 842 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1842 4030 4340 4486 5632 1842
Size: 2188 310 146 1146 2032
Fragments arranged by size:
2188 2032 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2834 2834
Size: 5822
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1154 1560 1782 2022 2407 2821 3647 4079 4826
Size: 406 222 240 385 414 826 432 747
Cuts at: 4826 5094 1154
Size: 268 1882
Fragments arranged by size:
1882 826 747 432 414 406 385 268
240 222
EcoNI CCTnn'n_nnAGG
Cuts at: 682 682
Size: 5822
EcoO109I rG'GnC_Cy
Cuts at: 348 376 2382 2450 2961 348
Size: 28 2006 68 511 3209
Fragments arranged by size:
3209 2006 511 68 28
EcoRI G'AATT_C
Cuts at: 2826 2826
Size: 5822
EcoRV GAT'ATC
Cuts at: 1098 1098
Size: 5822
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 144 3841 144
Size: 3697 2125
FspI TGC'GCA
Cuts at: 962 3603 962
Size: 2641 3181
HaeII r_GCGC'y
Cuts at: 115 1695 1757 1853 1866 2180 2216 2395 3256
Size: 1580 62 96 13 314 36 179 861
Cuts at: 3256 3504 4526 5505 115
Size: 248 1022 979 432
Fragments arranged by size:
1580 1022 979 861 432 314 248 179
96 62 36 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 1396 1428 1693 1725 2242 2274 3664
Size: 32 1090 32 265 32 517 32 1390
Cuts at: 3664 3694 3696 3726 274
Size: 30 2 30 2370
Fragments arranged by size:
2370 1390 1090 517 265 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 758 2571 2586 2861 758
Size: 1813 15 275 3719
Fragments arranged by size:
3719 1813 275 15
HpaI GTT'AAC
Cuts at: 2571 2586 2571
Size: 15 5807
KasI G'GCGC_C
Cuts at: 111 1862 2176 2212 3252 3500 111
Size: 1751 314 36 1040 248 2433
Fragments arranged by size:
2433 1751 1040 314 248 36
KpnI G_GTAC'C
Cuts at: 2842 2842
Size: 5822
MluI A'CGCG_T
Cuts at: 1532 5255 1532
Size: 3723 2099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 713 1427 1490 1691 1772 2273 2384 4493 4677
Size: 714 63 201 81 501 111 2109 184
Cuts at: 4677 713
Size: 1858
Fragments arranged by size:
2109 1858 714 501 201 184 111 81
63
MscI TGG'CCA
Cuts at: 241 2146 2533 3583 241
Size: 1905 387 1050 2480
Fragments arranged by size:
2480 1905 1050 387
MslI CAynn'nnrTG
Cuts at: 814 1300 1342 2626 3811 3938 814
Size: 486 42 1284 1185 127 2698
Fragments arranged by size:
2698 1284 1185 486 127 42
MspA1I CmG'CkG
Cuts at: 645 1920 2227 2256 2283 2430 2933 3247 3607
Size: 1275 307 29 27 147 503 314 360
Cuts at: 3607 4620 4865 645
Size: 1013 245 1602
Fragments arranged by size:
1602 1275 1013 503 360 314 307 245
147 29 27
NaeI GCC'GGC
Cuts at: 889 1861 2715 4003 889
Size: 972 854 1288 2708
Fragments arranged by size:
2708 1288 972 854
NarI GG'CG_CC
Cuts at: 112 1863 2177 2213 3253 3501 112
Size: 1751 314 36 1040 248 2433
Fragments arranged by size:
2433 1751 1040 314 248 36
NcoI C'CATG_G
Cuts at: 107 1343 2621 107
Size: 1236 1278 3308
Fragments arranged by size:
3308 1278 1236
NdeI CA'TA_TG
Cuts at: 1010 1010
Size: 5822
NgoMIV G'CCGG_C
Cuts at: 887 1859 2713 4001 887
Size: 972 854 1288 2708
Fragments arranged by size:
2708 1288 972 854
NheI G'CTAG_C
Cuts at: 2692 2692
Size: 5822
NotI GC'GGCC_GC
Cuts at: 2189 2189
Size: 5822
NspI r_CATG'y
Cuts at: 967 2661 2875 3375 4282 967
Size: 1694 214 500 907 2507
Fragments arranged by size:
2507 1694 907 500 214
PasI CC'CwG_GG
Cuts at: 664 664
Size: 5822
PciI A'CATG_T
Cuts at: 2657 4278 2657
Size: 1621 4201
PflMI CCAn_nnn'nTGG
Cuts at: 942 942
Size: 5822
PfoI T'CCnGG_A
Cuts at: 2038 2038
Size: 5822
PmeI GTTT'AAAC
Cuts at: 2821 2821
Size: 5822
PmlI CAC'GTG
Cuts at: 627 2142 2643 627
Size: 1515 501 3806
Fragments arranged by size:
3806 1515 501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4961 4993 4961
Size: 32 5790
PpuMI rG'GwC_Cy
Cuts at: 348 2382 2450 348
Size: 2034 68 3720
Fragments arranged by size:
3720 2034 68
PspXI vC'TCGA_Gb
Cuts at: 1022 1022
Size: 5822
PstI C_TGCA'G
Cuts at: 264 1727 2399 2869 3554 264
Size: 1463 672 470 685 2532
Fragments arranged by size:
2532 1463 685 672 470
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5822
PvuII CAG'CTG
Cuts at: 645 3247 3607 645
Size: 2602 360 2860
Fragments arranged by size:
2860 2602 360
RsrII CG'GwC_CG
Cuts at: 4017 4017
Size: 5822
SacI G_AGCT'C
Cuts at: 2836 2836
Size: 5822
SacII CC_GC'GG
Cuts at: 1921 2228 2284 1921
Size: 307 56 5459
Fragments arranged by size:
5459 307 56
SalI G'TCGA_C
Cuts at: 2859 2859
Size: 5822
SanDI GG'GwC_CC
Cuts at: 348 2450 348
Size: 2102 3720
SapI GCTCTTCn'nnn_
Cuts at: 3845 4055 3845
Size: 210 5612
SbfI CC_TGCA'GG
Cuts at: 2869 2869
Size: 5822
ScaI AGT'ACT
Cuts at: 1201 5339 1201
Size: 4138 1684
SfcI C'TryA_G
Cuts at: 33 260 1091 1723 2055 2395 2769 2865 3550
Size: 227 831 632 332 340 374 96 685
Cuts at: 3550 4543 4734 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 831 685 632 374 340 332
227 191 96
SfiI GGCCn_nnn'nGGCC
Cuts at: 2227 2227
Size: 5822
SfoI GGC'GCC
Cuts at: 113 1864 2178 2214 3254 3502 113
Size: 1751 314 36 1040 248 2433
Fragments arranged by size:
2433 1751 1040 314 248 36
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5822
SgrAI Cr'CCGG_yG
Cuts at: 1859 1859
Size: 5822
SmaI CCC'GGG
Cuts at: 2298 2844 2298
Size: 546 5276
SmlI C'TyrA_G
Cuts at: 793 1022 1954 2977 3892 4384 4646 793
Size: 229 932 1023 915 492 262 1969
Fragments arranged by size:
1969 1023 932 915 492 262 229
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5822
SphI G_CATG'C
Cuts at: 967 2875 3375 967
Size: 1908 500 3414
Fragments arranged by size:
3414 1908 500
StuI AGG'CCT
Cuts at: 566 1795 566
Size: 1229 4593
StyI C'CwwG_G
Cuts at: 107 561 1343 1637 1757 2621 2956 3310 107
Size: 454 782 294 120 864 335 354 2619
Fragments arranged by size:
2619 864 782 454 354 335 294 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1290 1356 2148 3405 3538 3997 4206 1290
Size: 66 792 1257 133 459 209 2906
Fragments arranged by size:
2906 1257 792 459 209 133 66
TatI w'GTAC_w
Cuts at: 533 1199 1328 2372 5337 533
Size: 666 129 1044 2965 1018
Fragments arranged by size:
2965 1044 1018 666 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 340 372 611 643 854 886 2077 2109 5339
Size: 32 239 32 211 32 1191 32 3230
Cuts at: 5339 5371 340
Size: 32 791
Fragments arranged by size:
3230 1191 791 239 211 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 607 2158 3619 607
Size: 1551 1461 2810
Fragments arranged by size:
2810 1551 1461
XbaI T'CTAG_A
Cuts at: 2853 2853
Size: 5822
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 939 2229 2619 3317 939
Size: 1290 390 698 3444
Fragments arranged by size:
3444 1290 698 390
XhoI C'TCGA_G
Cuts at: 1022 1022
Size: 5822
XmaI C'CCGG_G
Cuts at: 2296 2842 2296
Size: 546 5276
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI ApaI AscI BaeI BcgI
BfrBI BsiWI BsmBI BstXI BstZ17I ClaI CspCI FseI
FspAI HindIII MfeI NruI NsiI PacI PshAI PsiI
PspOMI PsrI SexAI SnaBI SrfI SspI SwaI XmnI
ZraI