Restriction map for
pF1KB9742
(Circular) (Six-base) MAPSORT of: pF1KB9742.seq Check: 3144 from: 1 to: 4676
pF1KB9742 4676 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1714 1714
Size: 4676
Acc65I G'GTAC_C
Cuts at: 1692 1692
Size: 4676
AclI AA'CG_TT
Cuts at: 4516 4516
Size: 4676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 2933 3680 3948 2501
Size: 432 747 268 3229
Fragments arranged by size:
3229 747 432 268
AfeI AGC'GCT
Cuts at: 736 736
Size: 4676
AflII C'TTAA_G
Cuts at: 846 846
Size: 4676
AflIII A'CryG_T
Cuts at: 864 1060 1170 2028 3132 4109 864
Size: 196 110 858 1104 977 1431
Fragments arranged by size:
1431 1104 977 858 196 110
AgeI A'CCGG_T
Cuts at: 3064 3064
Size: 4676
AleI CACnn'nnGTG
Cuts at: 439 439
Size: 4676
AlwNI CAG_nnn'CTG
Cuts at: 1981 3548 1981
Size: 1567 3109
ApaLI G'TGCA_C
Cuts at: 269 333 903 3446 269
Size: 64 570 2543 1499
Fragments arranged by size:
2543 1499 570 64
ApoI r'AATT_y
Cuts at: 1680 4029 4285 1680
Size: 2349 256 2071
Fragments arranged by size:
2349 2071 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4676
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
AvaI C'yCGr_G
Cuts at: 537 1696 537
Size: 1159 3517
BamHI G'GATC_C
Cuts at: 1701 1701
Size: 4676
BanI G'GyrC_C
Cuts at: 377 541 1692 2106 2354 2389 377
Size: 164 1151 414 248 35 2664
Fragments arranged by size:
2664 1151 414 248 164 35
BanII G_rGCy'C
Cuts at: 205 218 1690 2720 205
Size: 13 1472 1030 2161
Fragments arranged by size:
2161 1472 1030 13
BbeI G_GCGC'C
Cuts at: 381 2110 2358 381
Size: 1729 248 2699
Fragments arranged by size:
2699 1729 248
BciVI GTATCCnnnnn_n'
Cuts at: 2574 3335 4676 2574
Size: 761 1341 2574
Fragments arranged by size:
2574 1341 761
BclI T'GATC_A
Cuts at: 222 2196 222
Size: 1974 2702
BfrBI ATG'CAT
Cuts at: 1088 1088
Size: 4676
BglI GCCn_nnn'nGGC
Cuts at: 112 959 112
Size: 847 3829
BglII A'GATC_T
Cuts at: 2191 4228 2191
Size: 2037 2639
BlpI GC'TnA_GC
Cuts at: 1788 1788
Size: 4676
Bme1580I G_kGCm'C
Cuts at: 273 337 544 907 2301 2394 3450 273
Size: 64 207 363 1394 93 1056 1499
Fragments arranged by size:
1499 1394 1056 363 207 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 545 582 599 1345 1489 1519 1888 1969 2304
Size: 37 17 746 144 30 369 81 335
Cuts at: 2304 4448 545
Size: 2144 773
Fragments arranged by size:
2144 773 746 369 335 144 81 37
30 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3043 3043
Size: 4676
Bpu10I CC'TnA_GC
Cuts at: 199 199
Size: 4676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 816 1852 2731 3223 3521 816
Size: 1036 879 492 298 1971
Fragments arranged by size:
1971 1036 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 498 779 1093 1608 4380 498
Size: 281 314 515 2772 794
Fragments arranged by size:
2772 794 515 314 281
BsaAI yAC'GTr
Cuts at: 2029 2029
Size: 4676
BsaBI GATnn'nnATC
Cuts at: 6 2190 4227 6
Size: 2184 2037 455
Fragments arranged by size:
2184 2037 455
BsaHI Gr'CG_yC
Cuts at: 378 2107 2355 3057 4553 378
Size: 1729 248 702 1496 501
Fragments arranged by size:
1729 1496 702 501 248
BsaWI w'CCGG_w
Cuts at: 752 1865 2386 3064 3338 3485 3896 752
Size: 1113 521 678 274 147 411 1532
Fragments arranged by size:
1532 1113 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 673 703 673
Size: 30 4646
BseYI C'CCAG_C
Cuts at: 466 2101 2859 3436 3932 466
Size: 1635 758 577 496 1210
Fragments arranged by size:
1635 1210 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1019 1424 1019
Size: 405 4271
BsiEI CG_ry'CG
Cuts at: 104 2218 2264 3472 104
Size: 2114 46 1208 1308
Fragments arranged by size:
2114 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 205 273 337 907 1690 2468 2658 3450 205
Size: 68 64 570 783 778 190 792 1431
Fragments arranged by size:
1431 792 783 778 570 190 68 64
Bsp1286I G_dGCh'C
Cuts at: 205 218 273 337 544 907 1690 2301 2394
Size: 13 55 64 207 363 783 611 93
Cuts at: 2394 2468 2658 2720 3450 205
Size: 74 190 62 730 1431
Fragments arranged by size:
1431 783 730 611 363 207 190 93
74 64 62 55 13
BspEI T'CCGG_A
Cuts at: 3896 3896
Size: 4676
BspHI T'CATG_A
Cuts at: 777 3852 777
Size: 3075 1601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1726 1940 2242 2623 1726
Size: 214 302 381 3779
Fragments arranged by size:
3779 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2968 3022 4506 4671 50
Size: 2918 54 1484 165 55
Fragments arranged by size:
2918 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 232 500 1376 1616 2588 232
Size: 268 876 240 972 2320
Fragments arranged by size:
2320 972 876 268 240
BsrFI r'CCGG_y
Cuts at: 2674 2855 3064 2674
Size: 181 209 4286
Fragments arranged by size:
4286 209 181
BssSI C'ACGA_G
Cuts at: 2947 3305 2947
Size: 358 4318
BstAPI GCAn_nnn'nTGC
Cuts at: 341 2053 341
Size: 1712 2964
BstBI TT'CG_AA
Cuts at: 3037 3037
Size: 4676
BstXI CCAn_nnnn'nTGG
Cuts at: 441 441
Size: 4676
BstYI r'GATC_y
Cuts at: 1 1701 2168 2191 2526 2772 3784 3870 3882
Size: 1700 467 23 335 246 1012 86 12
Cuts at: 3882 4228 1
Size: 346 449
Fragments arranged by size:
1700 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 263 373 263
Size: 110 4566
BtgI C'CryG_G
Cuts at: 107 209 437 1271 107
Size: 102 228 834 3512
Fragments arranged by size:
3512 834 228 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 447 2626 2807 2970 447
Size: 2179 181 163 2153
Fragments arranged by size:
2179 2153 181 163
BtsI GCAGTG_nn'
Cuts at: 564 816 905 1517 1919 564
Size: 252 89 612 402 3321
Fragments arranged by size:
3321 612 402 252 89
DraI TTT'AAA
Cuts at: 1675 1675
Size: 4676
DraIII CAC_nnn'GTG
Cuts at: 948 948
Size: 4676
DrdI GACnn_nn'nnGTC
Cuts at: 2382 3240 2382
Size: 858 3818
EaeI y'GGCC_r
Cuts at: 418 1183 2261 2435 2826 2853 4198 418
Size: 765 1078 174 391 27 1345 896
Fragments arranged by size:
1345 1078 896 765 391 174 27
EagI C'GGCC_G
Cuts at: 2261 2261
Size: 4676
EarI CTCTTCn'nnn_
Cuts at: 1091 2699 2909 1091
Size: 1608 210 2858
Fragments arranged by size:
2858 1608 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2884 3194 3340 4486 2884
Size: 310 146 1146 3074
Fragments arranged by size:
3074 1146 310 146
EcoICRI GAG'CTC
Cuts at: 203 1688 203
Size: 1485 3191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 2933 3680 3948 2501
Size: 432 747 268 3229
Fragments arranged by size:
3229 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 136 136
Size: 4676
EcoO109I rG'GnC_Cy
Cuts at: 1815 1815
Size: 4676
EcoRI G'AATT_C
Cuts at: 1680 1680
Size: 4676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2695 2695
Size: 4676
FspI TGC'GCA
Cuts at: 2457 2457
Size: 4676
HaeII r_GCGC'y
Cuts at: 381 738 2110 2358 3380 4359 381
Size: 357 1372 248 1022 979 698
Fragments arranged by size:
1372 1022 979 698 357 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 671 703 2518 2548 2550 2580 671
Size: 32 1815 30 2 30 2767
Fragments arranged by size:
2767 1815 32 30 30 2
HincII GTy'rAC
Cuts at: 518 1410 1500 1715 518
Size: 892 90 215 3479
Fragments arranged by size:
3479 892 215 90
HindIII A'AGCT_T
Cuts at: 149 350 1646 149
Size: 201 1296 3179
Fragments arranged by size:
3179 1296 201
HpaI GTT'AAC
Cuts at: 1410 1410
Size: 4676
KasI G'GCGC_C
Cuts at: 377 2106 2354 377
Size: 1729 248 2699
Fragments arranged by size:
2699 1729 248
KpnI G_GTAC'C
Cuts at: 1696 1696
Size: 4676
MluI A'CGCG_T
Cuts at: 4109 4109
Size: 4676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3347 3531 3347
Size: 184 4492
MscI TGG'CCA
Cuts at: 1185 2437 1185
Size: 1252 3424
MslI CAynn'nnrTG
Cuts at: 330 439 574 632 698 1579 2665 2792 330
Size: 109 135 58 66 881 1086 127 2214
Fragments arranged by size:
2214 1086 881 135 127 109 66 58
MspA1I CmG'CkG
Cuts at: 1366 1787 2101 2461 3474 3719 1366
Size: 421 314 360 1013 245 2323
Fragments arranged by size:
2323 1013 421 360 314 245
NaeI GCC'GGC
Cuts at: 2857 2857
Size: 4676
NarI GG'CG_CC
Cuts at: 378 2107 2355 378
Size: 1729 248 2699
Fragments arranged by size:
2699 1729 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4676
NgoMIV G'CCGG_C
Cuts at: 2855 2855
Size: 4676
NsiI A_TGCA'T
Cuts at: 1090 1090
Size: 4676
NspI r_CATG'y
Cuts at: 868 1064 1088 1174 1632 1729 2229 3136 868
Size: 196 24 86 458 97 500 907 2408
Fragments arranged by size:
2408 907 500 458 196 97 86 24
PciI A'CATG_T
Cuts at: 864 1060 1170 3132 864
Size: 196 110 1962 2408
Fragments arranged by size:
2408 1962 196 110
PmeI GTTT'AAAC
Cuts at: 1675 1675
Size: 4676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 4644
PstI C_TGCA'G
Cuts at: 346 1723 2408 346
Size: 1377 685 2614
Fragments arranged by size:
2614 1377 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4676
PvuII CAG'CTG
Cuts at: 1366 2101 2461 1366
Size: 735 360 3581
Fragments arranged by size:
3581 735 360
RsrII CG'GwC_CG
Cuts at: 2871 2871
Size: 4676
SacI G_AGCT'C
Cuts at: 205 1690 205
Size: 1485 3191
SalI G'TCGA_C
Cuts at: 1713 1713
Size: 4676
SapI GCTCTTCn'nnn_
Cuts at: 2699 2909 2699
Size: 210 4466
SbfI CC_TGCA'GG
Cuts at: 1723 1723
Size: 4676
ScaI AGT'ACT
Cuts at: 4193 4193
Size: 4676
SfcI C'TryA_G
Cuts at: 33 342 1619 1719 2404 3397 3588 33
Size: 309 1277 100 685 993 191 1121
Fragments arranged by size:
1277 1121 993 685 309 191 100
SfoI GGC'GCC
Cuts at: 379 2108 2356 379
Size: 1729 248 2699
Fragments arranged by size:
2699 1729 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
SmaI CCC'GGG
Cuts at: 1698 1698
Size: 4676
SmlI C'TyrA_G
Cuts at: 831 846 1831 2746 3238 3500 831
Size: 15 985 915 492 262 2007
Fragments arranged by size:
2007 985 915 492 262 15
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4676
SphI G_CATG'C
Cuts at: 1632 1729 2229 1632
Size: 97 500 4079
Fragments arranged by size:
4079 500 97
StuI AGG'CCT
Cuts at: 652 837 652
Size: 185 4491
StyI C'CwwG_G
Cuts at: 107 1666 1810 2164 107
Size: 1559 144 354 2619
Fragments arranged by size:
2619 1559 354 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2259 2392 2851 3060 2259
Size: 133 459 209 3875
Fragments arranged by size:
3875 459 209 133
TatI w'GTAC_w
Cuts at: 4191 4191
Size: 4676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 429 461 673 705 4193 4225 429
Size: 32 212 32 3488 32 880
Fragments arranged by size:
3488 880 212 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 588 1341 2473 588
Size: 753 1132 2791
Fragments arranged by size:
2791 1132 753
XbaI T'CTAG_A
Cuts at: 1707 1707
Size: 4676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2171 2171
Size: 4676
XmaI C'CCGG_G
Cuts at: 1696 1696
Size: 4676
XmnI GAAnn'nnTTC
Cuts at: 652 826 652
Size: 174 4502
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BmgBI BmtI BpmI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI